Infrared Reflectance Spectroscopy Research Articles

Hydrolysis of methylphosphonic anhydride solid to methylphosphonic acid probed by Raman and infrared reflectance spectroscopies

Much is still unknown about the mechanisms and rates of environmental degradation of organophosphorous pesticides and agents.

The Influence of Ingredients, Corn Particle Size, and Sample Preparation on the Predictability of the Near Infrared Reflectance Spectroscopy

The Influence of Ingredients, Corn Particle Size, and Sample Preparation on the Predictability of the Near Infrared Reflectance Spectroscopy

Short-range structure, the role of bismuth and property-structure correlations in bismuth borate glasses.

Bismuth-containing borate glasses, xBi2O3-(1 - x)B2O3, were synthesized in the broad composition range 0.20 ≤ x ≤ 0.80 by melting in Pt crucibles and splat-quenching between two metal blocks. Infrared reflectance spectra, measured in the range 30-5000 cm-1, were transformed into absorption coefficient spectra and then deconvoluted into component bands to probe the glass structure as a function of composition. Integrated intensities of bands above 800 cm-1 were used in combination with mass and charge balance equations to quantify the short-range borate structure in terms of the molar fractions X4m, X4o, X3, X2, X1 and X0 for borate units BØ4-, BØ2O23-, BØ3, BØ2O-, BØO22- and BO33-, where Ø and O- denote bridging and non-bridging oxygen atoms. Borate tetrahedral units were found to be present in both the meta-borate, BØ4-, and ortho-borate, BØ2O23-, forms with BØ4- constituting the dominating tetrahedral species for 0.20 ≤ x ≤ 0.70. The BØ2O23- units prevail at higher Bi2O3 levels (x > 0.7), and coexist with their isomeric triangular borate species BO33- (BØ2O23- ⇌ BO33-). The present IR results for the total molar fraction of borate tetrahedral units, X4 = X4m + X4o, are in very good agreement with reported NMR results for the fraction of boron atoms in four-fold coordination, N4. Besides evaluating X4m and X4o, the present work reports also for the first time the fractions of all types of triangular borate species X3-n with n = 0, 1, 2 and 3. The IR region below 550 cm-1 was found to be dominated by the Bi-O vibrational activity in coexisting ionic (160-230 cm-1) and distorted BiO6 sites (330-365 cm-1 and 475-510 cm-1), a result reflecting the dual role of Bi2O3 as glass-modifier and glass-former oxide. The latter role dominates in glasses exceeding 60 mol% Bi2O3, and is consistent with the extended glass formation in the bismuth-borate system. The structural results were used to calculate the average number of bridging B-Ø bonds per boron center, the average Bi-O and B-O single bond energy, and the atomic packing density of the studied glasses. These properties vary approximately linearly with Bi2O3 content in the three regimes 0.2 ≤ x ≤ 0.4, 0.4 < x ≤ 0.6 and 0.6 < x ≤ 0.83, and contribute collectively to the composition dependence of glass transition temperature.

ИК-активные фононы ионного кристалла LiNiPO-=SUB=-4-=/SUB=-

The results of an experimental study of the phonon spectrum of a LiNiPO4 single crystal by IR reflection spectroscopy are presented. The spectroscopic parameters of IR phonons are obtained. The observed number of phonons is mainly in agreement with the performed group-theoretical analysis, and the reasons for small discrepancies are discussed. With decreasing temperature, a hardening of phonon frequencies is observed, associated with temperature changes in the parameters of the crystal lattice.

Visible Light Hydrogen Evolution over &amp;lt;i&amp;gt;α&amp;lt;/i&amp;gt;-MoO&amp;lt;/span&amp;gt;&amp;lt;sub&amp;gt;&amp;lt;span style=&amp;quot;font-family:Verdana;&amp;quot;&amp;gt;3&amp;lt;/span&amp;gt;&amp;lt;/sub&amp;gt;&amp;lt;span style=&amp;quot;font-family:Verdana;&amp;quot;&amp;gt; and &amp;lt;i&amp;gt;α&amp;lt;/i&amp;gt;-MoO&amp;lt;/span&amp;gt;&amp;lt;sub&amp;gt;&amp;lt;span style=&amp;quot;font-family:Verdana;&amp;quot;&amp;gt;3&amp;lt;/span&amp;gt;&amp;lt;/sub&amp;gt;&amp;lt;span style=&amp;quot;font-family:Verdana;&amp;quot;&amp;gt;/ZnO Hetero-Junction

MoO3 and 5% MoO3/ZnO were prepared by impregnation method using (NH4)6Mo7O24, 4H2O as precursor and ZnO as support. The prepared samples were characterized by X-ray diffraction (XRD), scanning electronic microscopy (SEM), infra red (FTIR) and UV-Vis diffuse reflectance (DRS) spectroscopies and photo-electrochemistry. The XRD pattern showed that the MoO3 powder treated at 700°C is a single-phase crystallizing in an orthorhombic structure with a direct optical transition (2.70 eV). The hetero-junction 5% MoO3/ZnO was photo-electrochemically characterized to assess its feasibility for H2 production under visible light. The capacitance potential (C-2 f(E)) characteristic of MoO3 plotted in Na2SO4, (0.1 M) electrolyte indicates n-type conduction with a flat band potential of -0.54 VSCE. The photocatalytic activity was performed for the photoreduction of water to hydrogen under visible light illumination. The best performance occurs at pH ~ 7 with an evolved volume of 5.9 mL.

Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy.

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures - plasmonic metasurfaces - as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, and lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane array detection system, we show that MEIRS also enables spectro-chemical imaging at the single-cell level. The described metasurface-based all-optical sensor opens the way to a scalable, high-throughput spectroscopic assay for live cells.

Light-Dependent Properties of Finishing and Leather Defects

The uniformity of the colour in leathers for the furnishing and automotive sectors is a particularly important feature, especially for white or light-coloured articles, intended to the high-end market; on these articles any colour alteration can be very visible and unpleasant, with possible technical and economic consequences for the producers. In a previous study, we analysed the possible causes behind the formation of some peculiar pink/salmon stains on different kinds of white/beige/light-coloured leather furnishing items, where we focalized our attention on TiO2 properties and its possible interaction with some organic-based antioxidants. In recent years, due to many other similar cases of defects that occurred to tanners and to upholstery traders, we decided to enhance the investigations concerning this topic. More in detail we analysed, defective leathers, powder pigments and chemicals, in order to identify the possible role of some substances in this issue, with particular reference to some antioxidants and aluminosilicates, where several diagnostic techniques have been utilised, as ATR-IR (Attenuated Total Reflectance IR Spectroscopy), SEM-EDX (Scanning Electron Microscopy-Energy Dispersive X-ray Analysis), XRF (X-ray Fluorescence spectrometry), GC-MS (Gas Chromatography-Mass Spectrometry), and DSC/TGA (Differential scanning calorimetry/Thermogravimetry) equipment.

ATR-Spin: an open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals.

Infrared spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of point-of-care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell culture media containing A549 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells μL-1 by using a single and cost-effective device and within a few minutes.

Estimation of relative feed value, relative forage quality and net energy lactation values of some roughage samples by using near infrared reflectance spectroscopy

The aim of this study is to determine the relative feed value (RFV), relative forage quality (RFQ) and net energy lactation (NEL) values of some roughage samples, based on the measurements taken from two different near infrared reflectance (NIR) devices. Corn silage (n = 18), alfalfa (n = 9), oat grass hay (n = 15), wheat straw (n = 10) were used as roughage samples and NIR measurements were taken from these samples in two ways. In the study, two different NIR (NIR1, NIR2) devices were used and nutrient values of roughage samples which were used were determined. The reference chemical analyzes of the roughage samples used in the study were made in the laboratory as dry matter. After the determination of the nutritional values, by adding these nutritional values (Dry matter, ether extract, crude protein, ash, neutral detergent fiber, acid detergent fiber) to the RFV, RFQ, NEL equations, RFV, RFQ and NEL values of each roughage sample were calculated. Meanwhile, the roughage samples used in the study were also analyzed in NIR devices and after the nutritional values were determined, these nutritional values were written in the RFV, RFQ, NEL equations thus RFV, RFQ, NEL values of each roughage samples were calculated separately for each device. Relationships between predictions obtained from NIR1 and NIR2 devices and reference chemical analysis values were determined by statistical tests. It was determined that the type of device and sample used had an important effect on the relationships between the results obtained from the reference analyzes and the predictions based on NIR measurements. Regression coefficients between RVF, RFQ and NEL values obtained from NIR1 and NIR2 devices and RVF, RFQ and NEL values obtained from reference (REF) analysis were determined to be 0.37 and 0.50. Among the roughage types, the highest similarity between NIR results and reference analysis results was found in alfalfa samples. The study results showed that indirect estimation by NIR relating to RVF, RFQ and NEL parameters is possible depending on the sample type and that there is a need for calibration improvement studies to determine these parameters directly with NIR.

Influence of Ni (II) oxime complex coupled with the combination of diverse sized ZnO nanoparticles on Photovoltaic Performance

The one side selective synthesis of quinoline carboxylic acid oxime complex was carried out successfully. The as-prepared quinoline carboxylic acid oxime complex was complexed with nickel (II) salts to form nickel (II) oxime complex. These complexes were further adsorbed onto ZnO films containing ZnO nanoparticles of various sizes. ZnO films containing a diverse proportion of ZnO nanoparticles were investigated to enhance the photovoltaic efficiency of the dye-sensitized solar cell. The as-synthesized complex was characterized by scanning electron microscopy (SEM), Ultra violet visible spectroscopy (UV-vis), Fourier Transform Infrared Spectroscopy (FT-IR) spectroscopy, 1Hydrogen Nuclear magnetic resonance spectroscopy (1HNMR), Liquid chromatography coupled with mass spectrometry (LC-MR), Brunauer–Emmett–Teller (BET), and Attenuated total reflection Infra-red spectroscopy (ATR-IR). The combination of large and small ZnO nanoparticles has significantly improves the photovoltaic efficiency. The optimum mixing ratio for the best performance (0.127%) of a dye-sensitized solar cell is achieved by mixing the small: large ZnO particles in a ratio 60:40. The increased efficiency is due to the harvesting of light caused by scattering effect from larger sized ZnO particles. The ZnO layer consisting of smaller particles which are very next to the ZnO bigger particles makes a good electronic contact between film electrode and the Indium-doped tin oxide glass substrate resulting in the increases in the dye molecules adsorption. The over-layered, large-sized ZnO particles enhance the light-harvesting by light scattering effect. Compared to the other mixtures of ZnO films, there is a decrease in the photovoltaic performance of the solar cell when ZnO particles (small and large in a ratio 1:1) were adsorbed onto the Ni (II) oxime complex, which are caused due to the decrease in the surface area and dye aggregation.

A Rapid Approach to Determine Soil Carbon Quality and Its Relationship to Soil Greenhouse Gas Emissions

ABSTRACT Soil organic matter composition controls many microbial processes in the soil matrix. How these processes interact to drive carbon cycling through greenhouse gas fluxes or carbon stabilization through biochemical transformations continues to evolve. From laboratory incubations, it is clear that low molecular weight compounds (LMWCs; e.g. dextrose, mannitol, and trehalose) can have a profound effect on microbially mediated fluxes. However, relationships between LMWCs and soil efflux have been largely unproven in field studies due to methodological constraints. In the current study we developed a novel use of Near Infrared Reflectance Spectroscopy (NIRS) to quantify LMWCs (explaining 38–51% of the variance). Paradoxically, we found dextrose concentrations to be negatively correlated with carbon dioxide fluxes in residential sites while mannitol was positively correlated with carbon dioxide fluxes in agricultural sites. Methane fluxes were strongly correlated with trehalose indicating a potential fungal interaction with bacterial methanogens. We found no clear link between LMWCs on nitrous oxide emissions suggesting inorganic nitrogen is a stronger limiting factor. The results from this study showed how a NIRS-based approach can improve mechanistic understanding of the drivers of soil greenhouse gas fluxes.

"Application of NIR Spectroscopy to Evaluate the Soil Fertility at the Field Scale"

The Near Infrared Reflectance Spectroscopy (VIS-NIRS) allows for quick analysis of soil abundance in basic macronutrients and development of fertilizer application maps. This article compares the results for pH and soil abundance in available forms of phosphorus, potassium and magnesium estimated using near-infrared spectroscopy in the 580-1850nm range and on the basis of standard laboratory methods. Soil samples were taken from a field of 20.6ha, from the surface of each polygon defined by a grid of squares with sides of 50x50m. The total soil sample (80) assigned to the square 50x50m taken from the arable layer to a depth of 0.2m. The constructed models must be then subjected to validation using an independent set of samples. In the presented experiment the samples were randomly divided into two equal sets, 40 samples each. A simple correlation analysis was conducted in order to estimate the correlations between the results obtained using standard methods and the spectrophotometry. The results were shown high relationship between values obtained by using NIRS and laboratory methods. Coefficients of determination computed for model calibration on the basis of linear regression, which for the soil pH, K content and subsequently for Mg and P was obtained, respectively: R2=0.90; R2=0.91; R2=0.83; R2=0.86. The results show the lower coherence of NIRS results with laboratory results for pH and K2O, followed by Mg and P2O5 for model verification. Determination coefficients in this case were as follows: R2=0.56; R2=0.54; R2=0.51 and R2=0.48. The fertility maps developed on the basis of laboratory analyses and by means of InfraXact™ device differed by 19% for phosphorus and 7,25% (relative root square error for area of fertility classes) for potassium.

NIRS to assess chemical composition of sheep and goat cheese

NIRS to assess chemical composition of sheep and goat cheese

PSVII-11 Fecal parameters as indicators of starch digestibility and feedlot performance of water buffaloes

Abstract This study evaluated fecal parameters indicative of starch digestibility of water buffaloes (Bubalus bubalis) of three genetic groups (GG: Jafarabadi, Mediterranean, and Murrah). Seventy-eight non-castrated males [n = 26 of each GG; 200 kg of initial body weight (BW); 210 days of age] were used. Animals were finished in feedlot, receiving a diet rich in starch [38.76% in dry matter (DM) basis]. Samples of feces, diet offered, and orts were collected for digestibility test during 3 consecutive days. Diet samples were incubated in situ in the rumen of cannulated buffaloes. The remaining material was submitted to analysis to quantify the indigestible neutral detergent fiber levels. Fecal starch (FS) determination was performed by near infrared reflectance spectroscopy. Final BW (BW, kg), metabolic BW (BW0.75, kg0.75), average daily gain (ADG, kg/day), DM intake (DMI, in kg/day, and %BW) were recorded. Data were analyzed using MIXED and CORR procedure in SAS, with significance considered if P ≤ 0.05. Starch digestibility and FS concentration are not influenced by GG (P ≥ 0.23), unlike fecal DM and fecal pH (P ≤ 0.04; Table 1). Although weak, negative correlations were detected between FS and BW (r = -0.247; P = 0.05) and BW0.75 (r = -0.261; P =0.03), as well as between fecal DM and BW (r = -0.245; P = 0.05), ADG (r = -0.341; P &amp;lt; 0.01), DMI, kg/day (r = -0.374; P &amp;lt; 0.01), and DMI, %BW (r = -0.335; P &amp;lt; 0.01) variables. The results demonstrate that there are differences among GG of water buffaloes regarding fecal parameters. Furthermore, the high FS content and the low fecal pH may indicate the low capacity of water buffaloes to digest starch. Thus, the research results provide evidence that water buffaloes are not able to use high-grain diets, and fecal parameters proved to be potential tools to evaluate the feedlot performance of these animals.

174 The Southeast hay contest: 10 year summary

Abstract The Southeast (SE) Hay Contest was started by a group of Extension agents from across the SE with the objective of increasing producer knowledge of the relative forage quality (RFQ) index. Since its inception, the program has grown to over 350 hay and baleage samples annually. Forage samples were categorized into warm-season perennial grass hay (WSPGH), alfalfa hay (AH), perennial peanut hay (PPH), cool-season perennial grass hay (CSPGH), mixed annual grass, or other hay (MAG), grass baleage (GB), and legume baleage (LB). Samples were analyzed for RFQ and crude protein (CP) using near infrared reflectance spectroscopy (NIRS) at the UGA Forage Testing Center (Athens, GA). The following summary includes data from 2009–2018 analyzed using Proc Glimmix of SAS 9.4 (SAS Inst., Cary, NC) with category and year as fixed effects and α = 0.05. Alfalfa hay had the greatest RFQ, followed by LB (P ≤ 0.004; 199 and 186, respectively). Perennial peanut and GB had median RFQ values (P ≤ 0.001; 169 and 150, respectively), and WSPGH had the least (P ≤ 0.008; 117). With the exception of 2017, the years 2013 to 2018 had the greatest RFQ (P &amp;lt; 0.001; 165). 2017 had a greater amount of rain than average, likely resulting in its lower average RFQ. Alfalfa hay had the greatest (P &amp;lt; 0.001) CP of all categories (23.9%; P &amp;lt; 0.001). Crude protein concentrations did not differ among WSPGH, CSPGJH, and MAG (P ≥ 0.192; 14.5%). While these forage samples were likely greater quality than samples not submitted, we conclude that outreach efforts, including the SE Hay Contest, have increased knowledge of the hay and baleage production process leading to increased hay quality from 2009 to 2018.

How to determine the complex refractive index from infrared reflectance spectroscopy?

A new theoretical methodology enabling the determination of the complex refractive index has been developed and explored; it only requires to use a classical set-up to control the incident angle, without the need for a polarizer. As a proof of concept, this new methodology has been successfully applied to investigate the optical properties of amorphous silica in the mid-infrared spectral range and validated by comparison with a standard routine based on polarization state of the light. This work thus makes it possible to determine the complex refractive index of an isotropic material by a reflectance measurement without needing to control the polarization of light. The refractive index can be measured with a relative uncertainty close to $$10^{-3}$$ over a wide range of wavelengths, as needed for a growing number of optical applications. Therefore, this new technique is cost effective and permits the accurate assessment of the refractive index in the whole infrared range.

Support effects in iridium-catalyzed aerobic oxidation of benzyl alcohol studied by modulation-excitation attenuated total reflection IR spectroscopy

The influence of the support (Al2O3, CeO2, TiO2) in the oxidation of benzyl alcohol (BA) to benzaldehyde on Ir-based catalysts was investigated by ATR-IR spectroscopy in tandem with modulation excitation spectroscopy (ATR-IR-MES) at working conditions of the catalysts. ATR-IR-MES unveiled the dissociative adsorption of BA and the formation of adsorbed transient alkoxy species (C6H5CH2O) to be key for high catalytic performance. These species were detected on Ir/TiO2 and Ir/Al2O3 but not on Ir/CeO2, which exhibited poor catalytic performance. On Ir/TiO2 pretreated with hydrogen at 450 °C about 84% of the Ir sites were blocked due to formation of a titania overlayer. However, this catalyst afforded a more than seven times higher activity than the corresponding one pretreated at 300 °C, where about 68% of the Ir sites were blocked. The high activity of the titania overlayer is attributed to the formation of Ti cations with oxygen vacancies caused by the hydrogen pretreatment.

Reflectance spectroscopy applied to clay mineralogy and alteration intensity of a thick basaltic weathering sequence in Hainan Island, South China

Visible/Near Infrared Reflectance spectroscopy (350–2500 nm) is an efficient and effective tool to identify and semi-quantify clay minerals, Fe3+ (oxyhydr)oxides, and their crystal-chemistry/structure. Even so, there have been few studies systematically describing how spectroscopy can be applied to mineralogical evolution during weathering. This study investigated reflectance spectra of a long core of a thick basaltic weathering sequence in Hainan Island and a series of transformation products from montmorillonite to kaolinite to present spectral features associated with weathering trends. In combination with X-ray diffraction and geochemical results, mineralogical transitions and weathering intensity of the core samples were described from a spectroscopic perspective. Several different protoliths were spectroscopically recognized in the Hainan weathering sequence, consistent with geological observation and geochemical analysis. Overprinted on these multiple protoliths was a discernable weathering sequence observable by infrared spectroscopic methods. Clay minerals changed upwards in section from TOT type (nontronite) to TO type (kaolinite), and finally to O type (gibbsite) hydroxide clay-size particles as the result of gradual hydrolysis of silicates and leaching of mobile elements with weathering. The relative abundance of hydroxyl to interlayer water, observable by the band depth ratio of spectral absorptions around 1400 nm and 1900 nm (BD1400/BD1900), increased progressively and correlated positively with the chemical index of alteration and Al2O3 concentration. The increasing BD1400/BD1900 index value with weathering was the result of gradual leaching of interlayer cations (e. g. Na+, K+), and their water complexes within interlayers, and gradual formation of M-OH layers by hydrolysis. Owing to leaching of Mg and release of Fe, the octahedral M-OH formed by hydrolysis was gradually dominated by Al-OH. Reflectance spectroscopy is a powerful tool to identify protolith changes in weathering sequences and for studying clay mineralogical transitions and weathering intensity. This spectroscopic approach could have great value for understanding weathering related to paleoclimate on Earth or other planets using field spectroscopy and remote sensing.

Thin Film Deposition by Atmospheric Pressure Dielectric Barrier Discharges Containing Eugenol: Discharge and Coating Characterizations.

Eugenol (4-Allyl-2-methoxyphenol) is the main constituent of clove oil. In addition to being widely used as a condiment, it has been recognized as a powerful bactericide. Owing to that, Eugenol has been used in several applications including odontology and as a conservative for food products. Aiming at the development of natural bactericide coatings, in this work, using an atmospheric pressure plasma in a dielectric barrier discharge (DBD) reactor Eugenol was deposited on stainless steel substrate, with argon as a carrier gas. The discharge power supply was a transformer at 14.4 kV peak-to-peak voltage and 60 Hz frequency. Operating with a gas flow rate at 4 L/min, the active power was around 1.2 W. The maximum plasma electron temperature of the plasma with monomers was about 1.5 eV, estimated by visible emission spectroscopy using a local thermodynamic equilibrium approach. The study also comprehended the analysis of the film structure, aging, and thermal stability using infrared reflectance spectroscopy, and its thicknesses and roughness by profilometry. The thickness of the films was in the range of 1000 to 2400 nm with a roughness of up to 800 nm with good adhesion on the substrate. The FTIR result shows a stable coating with a chemical structure similar to that of the monomer. Aging analysis showed that the film does not degrade, even after exposing the film for 120 days in ambient air and for 1.0 h under a high thermal UV-lamp.

Effectiveness of different approaches for in situ measurements of organic carbon using visible and near infrared spectrometry in the Poyang Lake basin area

AbstractResearch on soil carbon dynamics and content in the Poyang Lake basin area is important because of their effects on the soil carbon pool and soil carbon cycle. Visible and near infrared (VNIR) diffuse reflectance spectroscopy can provide analytical dense soil data reflecting multiple physical and chemical properties of soil under in situ conditions. The effective use of in situ VNIR in the Poyang Lake basin area would greatly improve the collection of high‐resolution and spatially explicit soil data and, thus, hasten the monitoring process. We collected two data sets S1 and S2 in 2016–2018. A dataset from S1 was used to develop the in situ correction matrix using laboratory and in situ spectra. Spectra from the Chinese Soil Spectroscopic Database (CSSD) and dataset of S1 in dry laboratory conditions were used for calibration (CSSD‐local calibration). The dataset from S2 was utilized as the validation dataset. Four in situ correction methods, external parameter orthogonalization (EPO), piecewise direct standardization, direct standardization, and generalized least squares weighting, were used to remove the in situ effect on the spectra. In addition, partial least squares regression and support vector machine (SVM), were used to test the effectiveness of the prediction. We also compared the prediction results by calibration of the CSSD, CSSD‐local and CSSD combined with four in situ samples from the validation datasets with extra‐weighting (CSSD‐EX). The results showed that EPO was the most effective method for removing the in situ effect. EPO and SVM with the calibration of CSSD‐EX yielded the best prediction result with the lowest root mean squared error at 1.71 g kg−1 and the highest Lin's concordance correlation coefficient at 0.88. The CSSD with EPO and SVM would allow the in situ estimation of soil organic carbon in the Poyang Lake basin area at a reasonable cost.