As an emerging field of e-commerce in China, fresh food e-commerce is still in the development period. In order to facilitate an in-depth study, this paper summarizes the operational mechanism and the characteristics of the fresh food e-commerce supply chain, and proposes suggestions about fresh food e-commerce operation and development. One of the challenges in the implementation of “agricultural and supermarket docking” mode is that fresh agricultural products circulation is not fluent. Public information service platform enables the enterprise information resources sharing within the scope of supply chain. It uses information technology tools to integrate the involved enterprise's information resources in supply chain with Internet advantages. In the logistics business, under the premise of sharing of information, retail supermarkets and third party cold chain logistics services company combine business situation and adjust the logistics business process. In the process of circulation of agricultural products, it works together in the process of transportation and distribution of agricultural products based on the public information service platform in both orders and waybill operation independently. Its systematic operation includes cold chain transportation and cold storage resource, reducing their operating cost, improving the benefit of enterprises and improving the service level. Through the application of Internet technology, it can solve the traditional information service platform of the quality of agricultural products and transportation problems.