Diverse land use patterns exhibit varying effects on water quality across different seasons and spatial scales. However, current studies on the correlation between land use and water quality in single small-scale basins no longer meet the needs of regional coordinated development. Simultaneous comparative analysis of multiple large-scale basins can promote water environmental protection in different basins, but there is currently limited relevant research. In this study, the data from 86 sampling points across seven major river basins in China were analyzed. Multivariate statistical analysis and the redundancy analysis (RDA) were employed to investigate the influence of land use patterns on water quality at different seasons and spatial scales. The results indicated notable differences in water quality across various seasons and locations. Except for higher pH and permanganate index (COD) concentrations in the wet season in the Songhua River Basin and higher COD concentrations in the Pearl River Basin, the concentrations of all parameters in other basins are higher in the dry season. PH and COD exhibited considerable spatial variations within basins, while dissolved oxygen (DO) and ammonia nitrogen (NH4+-N) showed smaller variations. RDA showed that land use had a more pronounced effect on water quality during the dry season in the Yangtze, Liao and Pearl River Basins, while the impact was greater in the wet season in the four basins of the Yellow, Huai, Hai and Songhua River Basins. At the spatial scale, the 2000 m buffer zone had the most significant impact within the Yangtze, Hai and Liao River Basins, while the 1000 m buffer zone had the greatest impact in the Huai and Pearl River Basins. For the Yellow and Songhua River Basins, the buffer zone was 500 m during the wet season and 1000 m and 2000 m during the dry season, respectively. The research findings can offer a scientific foundation for the development of basin-specific land management policies and water quality protection measures in different basins from a multi-scale perspective.