Experimental and theoretical studies have been completed in this paper to investigate the interface effect in the lubrication performance of a step slider bearing. Oleophobic fluorine-containing diamond-like carbon (F-DLC) coatings were prepared on the step slider to provide low affinity to lubrication films. With an optical slider bearing test rig, it is revealed that the F-DLC coating presents an increase in the film thickness/load-carrying capacity and a decrease in the coefficient of friction (COF), which is quite different from the common idea that both the load-carrying capacity and the COF are reduced at the same time. Contact angles measurement on the step slider shows that the affinity to the lubricant of the inlet step surface is lower than that of the outlet land, which can be attributed to the rougher surface of the step surface by the laser ablation. Theoretical analyses show that various interfacial slip velocities on the inlet step and the outlet land can generate different change in the load-carrying capacity and the COF. When the step surface presents lower interfacial slip velocity of the film than the land surface, increase in load-carrying capacity and decrease in the COF can be theoretically found to support the experiments finds.