Infectious Bovine Rhinotracheitis Antibodies Research Articles

102 Combination and individual vaccines for bovine viral diarrhea virus and infectious bovine rhinotracheitis effects on reproductive cyclicity and immune response

Abstract Vaccines are known to impact both the immune and reproductive systems. The objective of this research was to identify immunological and cyclic differences following vaccination for Bovine Viral Diarrhea Virus (BVDV) and/or Infectious Bovine Rhinotracheitis (IBR). Brahman cows (n = 15) were administered PGF2α to regress corpus lutea on d -3. Animals (d 0) were immunized (2mL, intramuscular injection) with one of three treatments: 1) Modified Live Vaccine (MLV) for BVDV and IBR (BVD+IBR; n = 5), 2) MLV for BVDV only (BVD; n = 5), or 3) sterile saline (CON; n = 5). Blood samples (30 mL/cow) were collected (d -3, 0, 2, 4, 6, 8, 10, and 14) and peripheral blood mononuclear cells (PBMC) were isolated. PBMC were incubated with propidium iodide and antibodies for specific surface cell markers (CD4, CD8, CD25, CD14, CD86, and CD335). An Amnis FlowSight flow cytometer determined cell type percentage. BVDV and IBR antibody concentrations were analyzed in serum samples (d -3, 0, and 14). Plasma samples (d 0, 2, 4, 6, 8, 10, and 14) were evaluated for interferon (IFN)-γ, interleukin (IL)-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17A, macrophage inflammatory protein (MIP)-1α, MIP-1β, IL-36RA, interferon-gamma-induced protein (IP)-10, monocyte chemoattractant protein (MCP)-1, tumor necrosis factor (TNF) α, and vascular endothelial growth factor (VEGF) A concentrations by a MagPix multiplex machine using a MILLIPLEX Bovine Cytokine/Chemokine Magnetic Bead Panel. Progesterone (d 0, 2, 4, 6, 8, 10, and 14) and estradiol (d 0) concentrations were determined by RIA. Differences in antibody titers, PBMC populations, and cytokine concentrations were analyzed as repeated measures using PROC MIXED (SASv9.4). Forty percent of BVD+IBR animals experienced abnormal estrus cycles, but no BVD or CON animals did. There was a treatment effect on BVD titer concentration with BVD animals having greater titer concentrations than CON (P = 0.02) but similar concentrations to BVD+IBR (P = 0.18). There was a treatment by time interaction (P < 0.0001) on IBR titers with BVD+IBR animals having greater titer concentrations on d 14 compared with BVD and CON. Treatment had no effect on leukocyte populations (P ≥ 0.1409), but all populations differed over time (P ≤ 0.0045). Antigen presenting cells (CD14+) and natural killer cells (CD335+) were affected by a treatment and time interaction (P ≤ 0.0479). BVD animals had increased CD14+ cells on d 2, 4, 8, and 14 compared with CON, but CON had a greater CD335+ cell population compared with BVD and BVD+IBR on d 10. There tended to be or was a significant treatment by day interaction for IL-1α (P = 0.08), IL-1b (P = 0.03), IL-10 (P = 0.06), IFN-γ (P = 0.05), IP-10 (P = 0.02), MIP-1α (P = 0.02), MIP-1 b (P = 0.01), TNFα (P = 0.01), and VEGFA (P = 0.03). IL-1a, IL-10, IL-1b, and MIP-1 b increased in BVD+IBR from d 0 to 4 or 6 then decreased but not in CON or BVD. Increased VEGFA was observed in CON compared with BVD and BVD+IBR animals. Overall, BVD+IBR resulted in abnormal cycles and changes in cytokine concentrations and leukocyte populations. USDA is an equal opportunity provider and employer.

Seroprevalence of Infectious Bovine Rhinotracheitis (IBR) in Indian Buffaloes

Background: Infectious bovine rhinotracheitis (IBR) is an infectious disease of bovines causing respiratory problem, abortions and reduced milk yield leading to huge economic losses. IBR is endemic in India. India ranks first in buffalo population. Reports on seroprevalence of IBR in cattle in India are available but not in buffaloes. The study was aimed at conducting a seroprevalence of IBR in Indian buffaloes. Methods: A total of 1000 buffalo serum samples collected randomly from top 10 milk producing states of India and were tested for IBR antibodies using Avidin-Biotin (AB) ELISA. Result: Cumulative seropositivity was found to be 43.30%. Punjab and Andhra Pradesh states showed highest (58%) and lowest (27%) seropositivity respectively and were part of north and south zone of the country. India has highest number of buffalo population. Presently, India do not vaccinate bovines against IBR and should take call on vaccination of dairy buffalo after having witnessed a high seroprevalence of IBR. This is the first report of such kind of number of buffalo samples tested for IBR antibodies.

Seroprevalence of Infectious bovine rhinotracheitis (IBR) in India: A 5-year study.

Infectious bovine rhinotracheitis (IBR) is a highly contagious disease of bovines causing respiratory symptoms, abortions, and reduced milk yield, leading to huge economic losses. Reports on seroprevalence in bovines in India are available and restricted to districts/states. In the present study, a nationwide seroprevalence of IBR in bovines was conducted to provide a national IBR seroprevalence to the Chief Veterinarian who in turn can design the control strategies. A total of 15,592 cattle and buffalo serum samples from 25 states and 3 Union Territories viz., Jammu and Kashmir, Puducherry, and Andaman and Nicobar Islands were tested for IBR antibodies using Avidin‑Biotin (AB) ELISA. Cumulative seropositivity was found to be 31.37%. Maharashtra and Rajasthan states, part of the west zone of the country, showed the highest and lowest seroprevalence, respectively. A total of 11,423 cattle and 4,169 buffalo serum samples were tested, which showed 33.91% and 24.39% seropositivity, respectively. India has the highest buffalo population. Presently, India no IBR vaccination programs are implemented in India. Considering the high seroprevalence, the authorities should plan control strategies for vaccinating dairy cows and buffaloes in India.

Impacts of a Post-Transport/Pre-Processing Rest Period on the Growth Performance and Serum Metabolites of Cattle Entering a Feedlot

Objective: The objective of this study was to evaluate the impact of a post-transport rest period on receiving calf growth performance and blood serum metabolites as indicators of immune function. Study Description: Eighty heifers were purchased from a sale barn and transported 6 hours to the Kansas State University Beef Cattle Research Center where they were processed at one of four times: immediately upon arrival or after a 6-, 24-, or 48-hour rest period. Cattle were then fed for 35 days with growth performance data collected weekly. Blood samples were also collected and analyzed for serum infectious bovine rhinotracheitis (IBR) titer and biochemical parameters. Results: Processing time did not impact (P > 0.05) heifer average daily gain. Overall, dry matter intake (DMI) decreased linearly (P = 0.027) as the rest time increased. The number of days for heifers to reach a targeted DMI of 2.5% body weight was linearly increased (P = 0.023) as time of rest increased. Serum IBR titer for heifers processed at either 0 or 6 hours upon arrival was higher (P < 0.01) on day 35 compared to day 0. This response was expected, as these cattle were vaccinated immediately or shortly after arrival. Interestingly, no difference in IBR titer was observed (P > 0.05) between day 0 and day 35 for heifers processed at either 24 or 48 hours upon arrival, indicating potential seroconversion of IBR antibodies before vaccination. The Bottom Line: These results indicate that rest time after arrival and prior to processing may not affect calf growth performance, but there is evidence that a 6-hour rest period could maximize DMI upon arrival to a feedlot. Additional research with greater replication and more industry-standard experimental conditions should be conducted to further evaluate these parameters.

SEROPREVALENCE OF INFECTIOUS BOVINE RHINOTRACHEITIS (IBR) IN NORTH EASTERN (NE) STATES OF INDIA

Infectious bovine rhinotracheitis (IBR) is an infectious disease caused by BoHV-1 and belongs to the Herpesviridae family. IBR is endemic in India including north eastern states of the country. Hence the study was undertaken to understand the seroprevalence of IBR in north eastern parts of the country. A total of 3125 cattle (Holstein Friesian crossbred) serum samples from 35 districts of five north eastern states (Assam, Manipur, Meghalaya, Mizoram, and Sikkim) of India were screened for infectious bovine rhinotracheitis (IBR) virus antibodies using Avidin biotin ELISA. A two-stage random sampling methodology was followed for the collection of samples. Results from the present study revealed that the overall seropositivity was reported around 29.50% while the highest and lowest seropositivity of 43.39% and 16.66% were reported in the states of Sikkim and Assam respectively, followed by Mizoram (42.16%), Manipur (29.86%) and Meghalaya (27.40%). Cattle of higher age groups showed the highest seropositivity compared to younger ones. A higher percent of IBR antibodies in cattle of NE states is a cause of concern and a detailed study on IBR prevalence comprising of a large number of the bovine population need to be undertaken.

Prevalencia del virus de la rinotraqueitis infecciosa bovina en el departamento del Caquetá, Amazonia Colombiana

La rinotraqueitis infecciosa bovina (IBR) es una enfermedad ampliamente difundida enel mundo con gran repercusión socio-económica en las ganaderías bovinas. El objetivodel presente estudio fue determinar la prevalencia de IBR en 100 hatos del departamentodel Caquetá, para lo cual, se muestrearon 960 bovinos mayores de 36 meses entre eneroy marzo de 2016 en predios seleccionados a partir de los criterios: a) tamaño (50-180hectáreas), b) con más de 10 vacas en ordeño, c) disponibilidad de los productores paracooperar y d) accesibilidad de las vías. Las muestras de suero sanguíneo se remitieronal Laboratorio de Diagnóstico Clínico Veterinario del ICA y se analizaron a través laprueba Elisa de bloqueo (BHV-1) gB. Los sueros con porcentaje de bloqueo superior al55% se consideraron positivos a anticuerpos para IBR. Se encontró alta seroprevalencia(73,13%), mayor además en machos que en hembras (p &lt; 0,05). A nivel municipal laseroprevalencia fue mayor en los municipios de El Doncello, Puerto Rico y San Vicentedel Caguán y se encontró diferencia significativa (p &lt; 0,05) entre los nueve municipiosanalizados. A nivel de hatos, la prevalencia fue del 99%. En conclusión, la prevalenciadel virus de la rinotraqueitis infecciosa bovina (IBR) en bovinos de doble propósito deldepartamento del Caquetá fue muy alta.

A comparative evaluation of avidin-biotin ELISA and micro SNT for detection of antibodies to infectious bovine rhinotracheitis in cattle population of Odisha, India

AbstractAim:MaterialsandMethods:Results:Conclusion:Keywords The present study was undertaken to serologically d etect Infectious Bovine Rhinotracheitis (IBR) in the cattlepopulation of Odisha, India using (micro SNT) and(ABELISA)andfindingouttheircomparativeefficacytoserveasasuitablediagnostictoolinfieldcondition.Thestudywascarriedoutusingserumsamples(n=180)collectedrandomlyfromcattlepopulationsof nine districts of Odisha. Similarly vaginal swabs (n=26) from cattle having history of repeat breeding, abortion, vulvo-vaginitis and nasal swabs (n=8) from calves with respiratory symptoms and nasal discharge were collected aseptically, toascertainthecirculationofvirusamongthecattlepopulation.Virus isolation by cell culture and subsequent confirmation by polymerase chain reaction confirmed four isolates.Screening of serum samples revealed 9.44% and 12.22% samples positive for IBR antibodies in micro SNT andAB ELISArespectively.ThesensitivityandspecificityofABELISA testwasfoundtobe88.23%and95.70%respectivelytakingmicroSNTasgoldstandardandthekappavaluebetweenthetwotestswas0.75.Screeningofserumsamplesrevealed9.44%and12.22%samplespositiveforIBRantibodiesinmicroSNTandAB ELISA respectively, thus highlighting the circulation of virus among the livestock population of Odisha and that ABELISA could be more efficiently applied for the sero-diagnosis of IBR virus infections at field conditions, with demand formorestudyonfaster,efficientandlargescalescreeningoftheinfectedanimals.: infectious bovine rhinotracheitis, serumneutralizationtest,Odisha.avidin-biotin ELISA, bovine herpes virus -1, cattle, isolation,micro-Serum neutralization test Avidin-Biotin Enzyme linked immunosorbentassay

EFFECTS OF SUPPLEMENTAL LYSINE ON PERFORMANCE, ANTIBODY TITER AND RECTAL TEMPERATURE IN RESPONSE TO A MODIFIED-LIVE VIRAL VACCINE IN NEONATAL CALVES

Infectious Bovine Rhinotracheitis (IBR), caused by bovine herpesvirus-1, contributes significantly to economic losses in the dairy and beef cattle industry. Lysin e has been shown reduce virulence of herpesviruses in felids and humans. Our objective was to evaluate the effects o f supplemental lysine on serum IBR antibody titer a nd rectal temperature in response to a modified-live Intranas al (IN) or Intramuscular (IM) respiratory-virus vac cination. Sixty-four neonatal Holstein bull calves (7±2 d of age; BW = 37±4.2 kg) were used in a completely randomized design. Calves were fed milk replacer supplemented with either 17 g/d L-lysine monohydrochloride (LYS; 28 calves) or an equivalent amount of casein (CAS; 28 calves) for 42 d. Calves were then vaccinated with either an IN IBR-Parainfluenza virus-3 (PI3) or an IM (IBR-PI3-bovine viral diarrhea type I and II, bovine respi ratory syncytial virus) modified-live vaccine on d 36. A c ontrol group (8 calves) received no supplement or v accination. All calves were housed in individual calf pens (1.2 ◊2.1 m). Daily feed intakes were monitored and BW measured weekly. Calves were bled on d 0, 35, 36, 37 and 42. Temperature data loggers were attached to rectal p robes and temperatures were recorded every 5 min from d 28 to d 42. No significant differences were determined f or average performance, rectal temperature, or IBR antibody titers with either IN or IM vaccinations betw een LYS and CAS treated calves (p>0.10). However, serum urea nitrogen and the ratio of serum lysine: Arginine increased (p<0.05) for LYS compared to CAS calves. These results suggest that supplementing lysine impacts nitro gen metabolism but does not alter the response to IBR v accination or animal performance in neonatal Holstein calves.

Epidemiological Studies on Infectious Bovine Rhinotracheitis (IBR) in Different Parts of India

Bovine herpesvirus-1 (BHV-1), the causative agent of Infectious Bovine Rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine semen and can cause various syndromes like infectious bovine rhinotracheitis (IBR), pustular vulvovaginitis (IPV), encephalomyelitis, mastitis and affects respiratory, ocular, reproductive, alimentary, integumentary and central nervous systems besides causing neonatal infections. The BHV-1 infection has already been reported by seroprevalence studies in India amongst exotic and crossbred cattle at some organized farms. The current work was designed to study the prevalence of IBR using serological and molecular techniques, respectively in serum and semen samples, collected from different parts of India. IBR antibodies were diagnosed from 1010 serum sample by serum neutralization test and noticed 32.25% seroprevalence with maximum prevalence in Uttar Pradesh and minimum in Himachal Pradesh. Four percentage of semen samples were found positive for BHV-1 virus.

Dynamics of infection and immunity in a dairy cattle population undergoing an eradication programme for Infectious Bovine Rhinotracheitis (IBR)

Several countries within the European Union (EU) have successfully eradicated Infectious Bovine Rhinotracheitis (IBR), while others (e.g. Germany) are making efforts to achieve IBR-free status. EU member states IBR eradication programmes must meet Community legislation requirements that ban breeding farms from purchasing positive animals, from using whole-virus IBR vaccines, and from inseminating cows with semen from positive bulls. A follow-up study from 2002 to 2005 was carried out in the province of Trento (Italy), where a compulsory programme for IBR eradication was started in 1998. IBR outbreaks (identified on the basis of seroconversion of sentinel animals) were concentrated in larger positive herds. A higher incidence was recorded between 2003 and 2004. An association between markedly high temperatures in the summer of 2003 and virus reactivation has been suggested but is yet to be confirmed. The practice of driving cattle to common alpine pastures for the summer season did not play a significant epidemiological role in IBR transmission. Premising that only seronegative animals are allowed to enter dairy farms, animal movement increases the infection risk to a moderate extent. The long-term persistence of IBR antibodies was more pronounced in animals positive for antibodies to the glycoprotein E (gE). Scattered seroconversions, occurring mostly in positive herds, require careful interpretation in order to avoid overestimating the incidence of the infection at herd level.

A serological survey on the prevalence of infectious bovine rhinotracheitis in Buckinghamshire, Berkshire and Oxfordshire

Of the 2368 cattle sera from herds in Buckinghamshire, Berkshire and Oxfordshire examined for infectious bovine rhinotracheitis antibody by an indirect haemagglutination test, 162 (6.8 per cent) proved positive. Positive titres were more prevalent in older cows and they tended to show higher titres. There was little evidence of infection in the bulls tested. The percentage of positive animals in Berkshire and Oxfordshire was 4.7 compared with 10.5 in Buckinghamshire. The infection was not known to be associated with any clinical disease.

Prevalence of bovine virus diarrhoea and infectious bovine rhinotracheitis antibodies in Nigerian sheep and goats

Neutralising antibodies to bovine virus diarrhoea virus were commoner in Nigerian sheep than goats while precipitating antibodies offered an alternative but less reliable indicator of previous infection. In contrast, neutralising antibodies to infectious bovine rhinotracheitis virus were more common in goats than sheep. These findings are discussed in relation to infectivity rates in cattle and general husbandry practices.

Infectious bovine rhinotracheitis virus infection in bulls, with special reference to preputial infection.

In an experiment with infectious bovine rhinotracheitis (IBR) virus in two bulls, observed over a period of 122 weeks, the pattern of virus release was studied. Recurrent, unprovoked release of virus was demonstrated after one year in a nasal washing from one of the bulls and in preputial washings of both on 13 and 4 occasions, respectively, and finally in weeks 113 and 110, although clinical disease was not observed. During periods of recurrent virus release, concentrations of virus in the prepuce were generally much lower than during the period of primary infection; usually, however, they were not of negligible titer. The frequency of such periods and the virus titers observed strongly suggest that an IBR antibody carrier should always be considered as a potential source of infection to other animals. When virus was demonstrated in semen an almost equal amount was found in the preputial washing (50 ml). In week 120, virus replication in the respiratory tract and prepuce was induced in both bulls by prednisolone injections. It is concluded that antibody carriers will rarely attain a state of absolute immunity.

Infectious Bovine Rhinotracheitis Virus Infection in Bulls, with Special Reference to Preputial Infection

In an experiment with infectious bovine rhinotracheitis (IBR) virus in two bulls, observed over a period of 122 weeks, the pattern of virus release was studied. Recurrent, unprovoked release of virus was demonstrated after one year in a nasal washing from one of the bulls and in preputial washings of both on 13 and 4 occasions, respectively, and finally in weeks 113 and 110, although clinical disease was not observed. During periods of recurrent virus release, concentrations of virus in the prepuce were generally much lower than during the period of primary infection; usually, however, they were not of negligible titer. The frequency of such periods and the virus titers observed strongly suggest that an IBR antibody carrier should always be considered as a potential source of infection to other animals. When virus was demonstrated in semen an almost equal amount was found in the preputial washing (50 ml). In week 120, virus replication in the respiratory tract and prepuce was induced in both bulls by prednisolone injections. It is concluded that antibody carriers will rarely attain a state of absolute immunity.