Abstract
AbstractAim:MaterialsandMethods:Results:Conclusion:Keywords The present study was undertaken to serologically d etect Infectious Bovine Rhinotracheitis (IBR) in the cattlepopulation of Odisha, India using (micro SNT) and(ABELISA)andfindingouttheircomparativeefficacytoserveasasuitablediagnostictoolinfieldcondition.Thestudywascarriedoutusingserumsamples(n=180)collectedrandomlyfromcattlepopulationsof nine districts of Odisha. Similarly vaginal swabs (n=26) from cattle having history of repeat breeding, abortion, vulvo-vaginitis and nasal swabs (n=8) from calves with respiratory symptoms and nasal discharge were collected aseptically, toascertainthecirculationofvirusamongthecattlepopulation.Virus isolation by cell culture and subsequent confirmation by polymerase chain reaction confirmed four isolates.Screening of serum samples revealed 9.44% and 12.22% samples positive for IBR antibodies in micro SNT andAB ELISArespectively.ThesensitivityandspecificityofABELISA testwasfoundtobe88.23%and95.70%respectivelytakingmicroSNTasgoldstandardandthekappavaluebetweenthetwotestswas0.75.Screeningofserumsamplesrevealed9.44%and12.22%samplespositiveforIBRantibodiesinmicroSNTandAB ELISA respectively, thus highlighting the circulation of virus among the livestock population of Odisha and that ABELISA could be more efficiently applied for the sero-diagnosis of IBR virus infections at field conditions, with demand formorestudyonfaster,efficientandlargescalescreeningoftheinfectedanimals.: infectious bovine rhinotracheitis, serumneutralizationtest,Odisha.avidin-biotin ELISA, bovine herpes virus -1, cattle, isolation,micro-Serum neutralization test Avidin-Biotin Enzyme linked immunosorbentassay
Highlights
Infectious bovine rhinotracheitis (IBR) is caused by Bovine Herpes Virus-1(BHV-1), a member of the genus Varicellovirus in the sub-family Alphaherpesvirinae, family Herpesviridae and order Herpesvirales
Isolation of BHV-1 virus from clinical samples: In the present study attempt was made to isolate the virus from clinical samples like vaginal swabs and nasal swabs from animals with history of repeat breeding, abortion, pustular vulvovaginitis and animals with nasal lesions
After 24 h of incubation few cells were found to be rounded whose number increased after 48 hours and after 72 h syncitia development showing “bunch of grapes” appearance of virus infected cells was observed which was in correlation with previous BHV-1 isolation studies [9]
Summary
Infectious bovine rhinotracheitis (IBR) is caused by Bovine Herpes Virus-1(BHV-1), a member of the genus Varicellovirus in the sub-family Alphaherpesvirinae, family Herpesviridae and order Herpesvirales. Virions are enveloped and variably sized (approximately120250 nm in diameter), containing an icosahedral nucleocapsid composed of 162 capsomers. Genome is linear double-stranded DNA, 125–290 kbp in size [1].The virion contains about 70 proteins, of which 11 are envelope glycoproteins. Glycoprotein gB, gC and gD are of major importance followed byadditional glycoproteins (gE, gI, gH, gL, gG, gK and gM), thymidine kinase. BHV-1 virus has been divided into subtypes like BHV-1.1, BHV-1.2, and BHV-1.3. BHV- 1.1 is mostly related to the respiratory syndrome, BHV- 1.2 to genital infections, Copyright: The authors.
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