A comparative evaluation of avidin-biotin ELISA and micro SNT for detection of antibodies to infectious bovine rhinotracheitis in cattle population of Odisha, India

Abstract

AbstractAim:MaterialsandMethods:Results:Conclusion:Keywords The present study was undertaken to serologically d etect Infectious Bovine Rhinotracheitis (IBR) in the cattlepopulation of Odisha, India using (micro SNT) and(ABELISA)andfindingouttheircomparativeefficacytoserveasasuitablediagnostictoolinfieldcondition.Thestudywascarriedoutusingserumsamples(n=180)collectedrandomlyfromcattlepopulationsof nine districts of Odisha. Similarly vaginal swabs (n=26) from cattle having history of repeat breeding, abortion, vulvo-vaginitis and nasal swabs (n=8) from calves with respiratory symptoms and nasal discharge were collected aseptically, toascertainthecirculationofvirusamongthecattlepopulation.Virus isolation by cell culture and subsequent confirmation by polymerase chain reaction confirmed four isolates.Screening of serum samples revealed 9.44% and 12.22% samples positive for IBR antibodies in micro SNT andAB ELISArespectively.ThesensitivityandspecificityofABELISA testwasfoundtobe88.23%and95.70%respectivelytakingmicroSNTasgoldstandardandthekappavaluebetweenthetwotestswas0.75.Screeningofserumsamplesrevealed9.44%and12.22%samplespositiveforIBRantibodiesinmicroSNTandAB ELISA respectively, thus highlighting the circulation of virus among the livestock population of Odisha and that ABELISA could be more efficiently applied for the sero-diagnosis of IBR virus infections at field conditions, with demand formorestudyonfaster,efficientandlargescalescreeningoftheinfectedanimals.: infectious bovine rhinotracheitis, serumneutralizationtest,Odisha.avidin-biotin ELISA, bovine herpes virus -1, cattle, isolation,micro-Serum neutralization test Avidin-Biotin Enzyme linked immunosorbentassay