Gastrointestinal Nematode Infections Research Articles

Repeatability of Health and Welfare Traits and Correlation with Performance Traits in Dairy Goats Reared under Low-Input Farming Systems.

The objectives of the study were to estimate the repeatability of health and welfare traits and investigate their association with performance in three breeds of dairy goats reared under low-input farming systems in Greece. A total of 1210 goats of Eghoria (n = 418), Skopelos (n = 429), and Damascus (n = 363) breeds were assessed. Udder health, parasitic resistance, welfare, milk yield and quality, and body condition score were recorded monthly for two milking periods. Udder health records included somatic cell count (SCC) and total viable count (TVC). Based on combinations of SCC and TVC and thresholds set at >106 cells/mL and >2 × 104 cfu/mL, respectively, additional udder health phenotypes were defined. Parasitism included myiasis, tick infestation, gastrointestinal nematode (GIN) and cestode faecal egg count (FEC), and lungworm faecal larval count (FLC). Infection with each of the endoparasites was defined based on FEC/FLC. Welfare assessment parameters included the presence of ear and horn injuries, ocular and nasal discharge, body and udder abscesses, injury and lesions on the skin of different regions, diarrhoea, hernias, overgrown hooves, arthritis, lameness, and udder asymmetry. Trait repeatability and animal correlations were estimated. Significant (p < 0.05) repeatability was reported for all udder health and most welfare traits in all breeds, GIN and cestode FEC, and GIN and lungworm infection in Eghoria, and myiasis in Skopelos. Correlations of health and most of welfare traits with performance were non-significant or favourable. Overall, results demonstrate potential to improve health and welfare of the studied breeds without compromising performance.

Monitoring environmental conditions on the speed of development and larval migration of gastrointestinal nematodes in Urochloa decumbens in northeastern Brazil.

The study aimed to evaluate the speed of development of gastrointestinal nematodes (GIN) eggs to infective larvae (L3) and its migration under effect of meteorological variables: temperature, rainfall, relative humidity, solar radiation to Urochloa (=Brachiaria) decumbens grass pasture during rainy season, from June to September 2019, and dry season, October to March 2020, in Recôncavo baiano region of Bahia state, Brazil. Monthly, fecal samples obtained from goats with recurrent GIN infection were deposited in six plots of one square meter. In +7, +14 and+21-days post-deposition, lower and upper strata grass (0-15 and>15cm, respectively) and remaining feces were collected and submitted to Baermann's technique modified to perform larval count and identification. Meteorological data were obtained from a local weather station database. The log-transformed larval count results were analyzed regarding the collection day effect, stratum effect, comparing the means by Tukey's test (p<0.05). Multivariate regression analysis and correlation of meteorological variables with larval counts was performed. In the rainy season, the largest proportion of recovered L3 was concentrated in +7-days post-deposition in the months of June and July, both in the remaining feces and herbage samples, while in August development took place more slowly, after +14-days post-deposition. During the dry season, L3 development only occurred after the first collection in January and February. Lower strata had higher proportion of recovery than in upper strata. Negative correlation was found for solar radiation. Greater rainfall in the rainy season compared to dry season was a favorable condition for high contamination of grass samples. However, reduced vertical migration to upper strata may have been influenced by low temperatures during the rainy season. Regarding the genera present in the fecal samples used in the experiment were found Haemonchus sp., Trichostrongylus sp. and Oesophagostomum sp. Thus, it is possible to conclude that in the region of the study, during the rainy season, there is a rapid larval development and the L3 are able to migrate more effectively to the grass. On the other hand, in the dry season, larval development tends to be slower, with less migration to the grass. Solar radiation can be useful for predicting months with the highest risk of infection. Therefore, prophylactic measures should be employed in goat herds during the rainy season whilst natural reduced pasture contamination in the dry season may favor animal maintenance for a longer time.

Early-onset immune response to Haemonchus contortus infection in resistant Santa Ines suckling lambs compared with susceptible Ile de France

Santa Ines, an indigenous Brazilian hair sheep, in comparison with European sheep breeds (e.g., Ile de France), show greater resistance against gastrointestinal nematode (GIN) infections, mainly to Haemonchus contortus. Here we bring novel findings that address some gaps regarding the resistance traits involved in the development of the immunity of young lambs in the first few weeks of life to H. contortus infection. This study aimed to compare parasitological, humoral, and local effector cell-mediated responses, also the histopathological differences in the abomasal mucosa of Santa Ines (SI) and Ile de France (IF) suckling lambs serially infected with H. contortus. Parasitological variables, local and circulating humoral immunity, and local cellular response were evaluated in naïve Santa Ines (n = 14) and Ile de France (n = 12) lambs, randomized into four groups: infected SI (n = 8), non-infected control SI (n = 6), infected IF (n = 8) and non-infected control IF (n = 4). Lambs from infected groups were first infected at 14 days old, and multiple infections were conducted every second day, until the age of 66 days old (52 days post first infection). In comparison with infected Ile de France, infected Santa Ines lambs had lower mean eggs per gram of faeces, lower total H. contortus worm burden, lower females’ length, greater abomasal lymph node weight, greater mucosal thickness in the fundus, and also higher counts of eosinophils in the fundus, and mast cells and globule leukocytes in both fundic and pyloric mucosa of the abomasum. Intrabreed differences were observed into the infected Santa Ines group, with three of the eight lambs classified as highly resistant for displaying H. contortus burden ranging only from 1 to 42 worms. Overall, Santa Ines suckling lambs showed great resistance against H. contortus infection in comparison with Ile de France lambs, being able to mount a robust innate immune response at an early age, and before weaning.

In vitro effect of three tropical plants on adult Haemonchus placei, an haematophagous nematode from cattle

BackgroundVernonia amygdalina (leaf), Garcinia kola (seed), and Leucaena leucocephala (seed) are three well-known tropical plants used in African ethnomedicine to reduce parasitic worm burdens and are potential sources of alternative solution for controlling parasitic helminths infection in grazing livestock. This study investigated extracts from these plants for anthelmintic activity against adult Haemonchus placei, an haematophagous nematode from cattle abomasa. Powdered plant materials were macerated in acetone and the crude acetone extracts evaluated for anthelmintic activity using H. placei adult worm motility assay. Afterwards, fresh sample of V. amygdalina was macerated successively in chloroform and acetone and the extracts evaluated for anthelmintic activity. The chloroform extract was subjected to phytochemical and FT-IR analyses and fractionated by vacuum liquid chromatography. Anthelmintic data were fitted to a nonlinear regression equation (Log [extract or fraction] vs. lethality; variable slope) to produce best-fit sigmoidal curves and LC50 values computed with associated uncertainty.ResultsOf the three tropical plants, only V. amygdalina was active against adult H. placei with best-fit LC50 of 6.51 mg/mL (95% CI: 5.32–7.75). Evaluation of the two extracts obtained by successive maceration showed that chloroform extract (LC50, 2.46 mg/mL, 95% CI: 1.87–3.28) was 11 times as potent as acetone extract (LC50, 27.01 mg/mL, 95% CI: 21.32–48.57) (α < 0.0001). Chromatographic fractionation of the chloroform extract yielded four fractions (FA-FD) with FB (LC50, 2.38 mg/mL, 95% CI: 1.76–3.28) 2.19 times as potent as FC (LC50, 5.21 mg/mL, 95% CI: 4.40–5.79) against H. placei, while FA and FD were inactive. Phytochemical evaluation of the chloroform extract revealed the presence of saponins, steroids, terpenoids, cardiac glycosides, and the absence of tannins, flavonoids, alkaloids, and anthraquinones. FT-IR structural analysis of chloroform extract indicated the presence of key functional groups which are chemical fragments/ structural motifs known to be present in the two major classes of bioactive compounds (sesquiterpene lactones and steroid glucosides) reportedly to be found in V. amygdalina.ConclusionsThe findings showed that chloroform extract of V. amygdalina leaf possessed relatively good anthelmintic activity against adult H. placei. This could be indicative of its potential usefulness as an anthelmintic phytomedicine to control gastrointestinal nematodes infection in cattle.Key highlightsExtracts of three different plant materials (one leaf, two seeds) were tested against adult Haemonchus placei in vitro;Chloroform extract of Vernonia amygdalina was 11 times as potent as acetone extract;Fractionation of the chloroform extract yielded a bioactive fraction responsible for about 90% of the total lethal effect of the chloroform extract.Bioprocessing of V. amygdalina leaf could produce phytomedicines for organic livestock farming.Graphical abstract

Differences in the protection elicited by a recombinant Teladorsagia circumcincta vaccine in weaned lambs of two Canarian sheep breeds

Gastrointestinal nematode (GIN) infections are a serious drawback on small ruminant production. Since anthelmintic resistance has extended, optimisation of alternative non-chemical control strategies has attracted interest. Recently, a prototype recombinant vaccine protected immunologically mature sheep from Texel-cross and Canaria Sheep breeds against Teladorsagia circumcincta. The level of protective immunity stimulated by the vaccine varied between individuals and with age. Previous studies suggest that Canaria Hair Breed (CHB) sheep is naturally resistant to GIN infection, with some evidence suggesting that this protection is present in young lambs. Here, we sought to enhance this resistance by immunising three-month-old CHB lambs with a T. circumcincta prototype recombinant vaccine. Following vaccination and a larval challenge period, levels of protection against T. circumcincta infection were compared in CHB lambs with Canaria Sheep (CS) lambs (a breed considered less resistant to GIN). Lambs from the resistant CHB breed appeared to respond more favourably to vaccination, shedding 63% fewer eggs over the sampling period than unvaccinated CHB lambs. No protection was evident in CS vaccinated lambs. At post-mortem, CHB vaccine recipients had a 68% reduction in mean total worm burden, and female worms were significantly shorter and contained fewer eggs in utero compared to unvaccinated CHB lambs. A higher anti-parasite IgG2 level was detected in immunised CHB lambs compared to unvaccinated control CHB animals, with data suggesting that IgA, globular leucocytes, CD45RA+, CD4+ and CD8+ T cells are implicated in this protective response. The development of effective immunity in vaccinated CHB lambs did not reduce lamb growth rate as immunised CHB lambs had a significantly higher average daily weight gain after challenge than their unvaccinated counterparts. Therefore, the protection of CHB lambs was enhanced by immunisation at weaning, suggesting a synergistic effect when combining vaccination with presumed genetic resistance.

Farmers' perception on the control of gastrointestinal parasites in organic and conventional sheep production in Sweden.

A questionnaire was sent to sheep owners in Sweden to get information about anthelmintic drug use. The survey also investigated how respondents experienced problems with gastrointestinal nematode infections (GIN) focusing on Haemonchus contortus. The response rate was 31% and included both conventional and organic farms. The use of anthelmintics was low (45%), among which a majority (76%) drenched ewes on a single occasion, mostly with ivermectin (59%) followed by albendazole (19%). Other drugs were used rarely, however, unawareness of GIN risk was high (19%), especially among respondents with few animals. Anthelmintic dose calculations were done after visual appraisal by 63% and 22% calibrated the equipment before drug delivery, which is worrying since underdosing is a risk factor for the development of anthelmintic resistance. Like with anthelmintics, the perceived risk for GIN increased with herd size both by conventional and organic farmers. Faecal examination for the presence of GIN was done by 65% of the respondents and, among their sheep, H. contortus was or had been diagnosed in 41% of the herds. Irrespective of new stock had been imported from other countries or not, common problems were reported by 5% and 7% of the organic and conventional producers, respectively. Land use and grazing management strategies differed more in relation to herd size than by production form, with a majority (47%) having their sheep grazed in several paddocks, or at least the lambs were moved when separated from the ewes at weaning (25%). In contrast set stocked grazing was mainly reported on smaller farms. Co-grazing with cattle and horses were also frequently reported irrespective of production form, but with cattle to a somewhat greater degree on larger organic farms. Wild cervids, especially roe deer, were frequently observed on sheep pastures (87%). The veterinary involvement was higher on organic (65%) than on conventional farms (53%), and only 5% considered advice unimportant. Still, some conventional and organic producers treated sheeps routinely without a prior diagnosis, against the national regulations. 46% of the respondents drenched new and replacement stock. In conclusion, although some differences were observed between conventional and organic producers, the divergences were mainly due to herd size categories. Furthermore, despite a high veterinary involvement, we identified factors which can contribute to anthelmintic use, such as poor quarantine procedures, and deworming routines that can contribute to anthelmintic resistance in H. contortus.

Understanding the role of wild ruminants in anthelmintic resistance in livestock.

Wild ruminants are susceptible to infection from generalist helminth species, which can also infect domestic ruminants. A better understanding is required of the conditions under which wild ruminants can act as a source of helminths (including anthelmintic-resistant genotypes) for domestic ruminants, and vice versa, with the added possibility that wildlife could act as refugia for drug-susceptible genotypes and hence buffer the spread and development of resistance. Helminth infections cause significant productivity losses in domestic ruminants and a growing resistance to all classes of anthelmintic drug escalates concerns around helminth infection in the livestock industry. Previous research demonstrates that drug-resistant strains of the pathogenic nematode Haemonchus contortus can be transmitted between wild and domestic ruminants, and that gastro-intestinal nematode infections are more intense in wild ruminants within areas of high livestock density. In this article, the factors likely to influence the role of wild ruminants in helminth infections and anthelmintic resistance in livestock are considered, including host population movement across heterogeneous landscapes, and the effects of climate and environment on parasite dynamics. Methods of predicting and validating suspected drivers of helminth transmission in this context are considered based on advances in predictive modelling and molecular tools.

Prevalence of major nematodes and human factors that affect infection in the zebra dove in a closed cage system.

Background and Aim:Roundworms cause infections in the avian population that lead to illness and poor production. The singing zebra dove is an economically important animal in the Indo-Malay region. The prevalence of these parasitic groups in zebra doves is unknown. This study estimated the prevalence and associated human risk factors of gastrointestinal nematode infections in zebra dove farming.Materials and Methods:A cross-sectional survey was conducted from January to April 2021. The study was conducted on 184 doves in three zebra dove farms. Fecal samples were collected from pooled zebra dove droppings. Major proportions and infection intensity of gastrointestinal nematodes were morphologically identified and morphometrically investigated. Associated human factors were assessed through the interview surveys among farmers.Results:Results showed that 36.96% of the zebra doves were infected. The primary nematodes were Ascaridia galli (34.78%), Heterakis gallinarum (6.52%), Trichostrongylus tenuis (2.17%), Syngamus spp. (4.35%), and Amidostomum spp. (2.17%). The primary human factors that contribute to parasitic infection were poor hygiene, food contamination with parasites, and inappropriate deworming.Conclusion:There was a high prevalence of gastrointestinal nematodes in the zebra dove in the close cage system. Human factors played key roles as risk factors, and improves farming management will help reduce parasitic infections. However, these nematodes may contribute to poor health status and poor productivity of zebra doves. Further extensive studies on clinical signs and pathological changes should be conducted.

Ethnobotanical and ethnoveterinary survey in Paraguay: Medicinal plants used for deworming

Gastrointestinal nematodes (GIN) infections, commonly called ―worms‖, affect both humans and animals. In veterinary medicine, the search for alternatives to control GIN is now considered a priority because of increasing resistances to commercial anthelmintics. The use of bioactive plants and related secondary metabolites is one of these alternatives. This study aimed to collect data on the use of medicinal plants (MPs) for humans and veterinary practices and at listing plant species with potential anthelmintic properties to select some plant species for further studies following three criteria (availability, originality and popularity). A cross sectional survey was conducted with a total of 384 participants, using semi-structured face-to-face interviews and a free list. To evaluate quantitative data of MPs, two ethnobotanical indices were calculated, the Salience index (SI) and the Use Value (UV). The importance and use of MPs in the population of study was high, 98% (372/383) of participants reported use MPs and 86% (321/384) mentioned knowledge of MPs used against GIN. The transmission of knowledge is not limited to the family environment. Sixteen plant species were listed belonging to 10 botanical families. The Asteraceae was the most popular botanical family. Six plants species were indicated for veterinary use. The four MPs selected for furthers studies were, namely: Kyllinga odorata Valh. (Cyperaceae), Cassia occidentalis L. (Fabaceae), Artemisia absinthium L. (Asteraceae), Verbena litoralis Kunth. (Verbenaceae). Evaluation of those MPs based on in vitro and in vivo assays are planned to validate the traditional local knowledge of these 4 plants.

Status of gastrointestinal nematode infections and associated epidemiological factors in sheep from Córdoba, Colombia

Gastrointestinal nematodes cause serious economic losses in sheep production systems. To determine the prevalence and risk factors associated with these parasites, a study was conducted on 595 sheep in Córdoba, Colombia. Prevalence and parasite burden were determined using the McMaster technique. Larvae were cultured from feces to identify the nematode genera. For the analysis of associated factors and parasite burden, data means, medians, and confidence intervals were compared. A georeferenced data analysis was performed and an epidemiological map was constructed. An overall prevalence of 88.2% was found, with the highest prevalence and parasite burden for Strongylida (83.2%) and Strongyloides (41%) nematodes. The mean parasite burden was 1255 eggs per gram. The gastrointestinal nematode genera identified were Trichostrongylus, Strongyloides, Haemonchus, Oesophagostomum, Bunostomum, and Cooperia. In general, a significant association was found between parasite burden and dewormed animals, anthelmintic used, date of last deworming, and breeds. The FAMACHA® method and body condition showed a significant association with the Strongylida parasite burden. Clusters with higher intensity of gastrointestinal nematode burden and high prevalence were observed in the regions of Bajo Sinú, Sinú Medio, and San Jorge.

Differential responses of abomasal transcriptome to Haemonchus contortus infection between Haemonchus-selected and Trichostrongylus-selected merino sheep.

Haemonchus contortus is the most prevalent and pathogenic gastrointestinal nematode infecting sheep and goats. The two CSIRO sheep resource flocks, the Haemonchus-selected flock (HSF) and Trichostrongylus-selected flock (TSF) were developed for research on host resistance or susceptibility to gastrointestinal nematode infection. A recent study focused on the gene expression differences between resistant and susceptible sheep within each flock, with lymphatic and gastrointestinal tissues. To identify features in the host transcriptome and understand the molecular differences underlying host resistance to H. contortus between flocks with different selective breeding and genetic backgrounds, we compared the abomasal transcriptomic responses of the resistant or susceptible animals between HSF and TSF flocks. A total of 11 and 903 differentially expressed genes were identified in the innate infection treatment in HSF and TSF flocks between resistant and susceptible sheep respectively, while 52 and 485 genes were identified to be differentially expressed in the acquired infection treatment, respectively. Among them, 294 genes had significantly different gene expression levels between HSF and TSF flock animals within the susceptible sheep by both the innate and acquired infections. Moreover, similar expression patterns of the 294 genes were observed, with 273 genes more highly expressed in HSF and 21 more highly expressed in the TSF within the abomasal transcriptome of the susceptible animals. Gene ontology enrichment of the differentially expressed genes identified in this study predicted the likely differing function between the two flock's susceptible lines in response to H. contortus infection. Nineteen pathways were significantly enriched in both the innate and adaptive immune responses in susceptible animals, which indicated that these pathways likely contribute to the host resistance development to H. contortus infection in susceptible sheep. Biological networks built for the set of genes differentially abundant in susceptible animals identified hub genes of PRKG1, PRKACB, PRKACA, and ITGB1 for the innate immune response, and CALM2, MYL1, COL1A1, ITGB1 and ITGB3 for the adaptive immune response, respectively. Our results offered a quantitative snapshot of host transcriptomic changes induced by H. contortus infection between flocks with different selective breeding and genetic backgrounds and provided novel insights into molecular mechanisms of host resistance.

Positivity Rate Investigation and Anthelmintic Resistance Analysis of Gastrointestinal Nematodes in Sheep and Cattle in Ordos, China.

Simple SummaryIn order to understand the positivity rates of gastrointestinal nematodes in cattle and sheep in Ordos, and the effects of different pasture types on the distribution of gastrointestinal nematodes, we conducted an epidemiological investigation and analysis in four banner districts of Ordos. The results showed that the positive rates of sheep and cattle were 38.84% and 4.48%, respectively. The anthelmintic resistance analysis revealed that the nematode population in the area was severely resistant to ivermectin and albendazole, and resistance to levamisole, nitroxynil and closantel was suspected.Gastrointestinal nematodes (GINs), such as Trichostrongylidae, are important pathogens in small ruminants, causing significant losses in these livestock species. Despite their veterinary importance, GINs have not been studied in certain regions of the world. Therefore, much of their epidemiology and economic impact on production remain unknown. In the present study, a systematic epidemiological survey based on the modified McMaster technique was conducted to investigate the type and infection of GINs in sheep and cattle. In 9622 fecal samples from 491 sampling sites in the four main banner districts of Ordos, the prevalence of GIN infection was found to be 38.84% and 4.48% in sheep and cattle, respectively. At the same time, the effects of four pasture types on the distribution of GINs were analyzed. This study also found severe resistance to ivermectin and albendazole in GINs and suspected anthelmintic resistance in nitroxynil, levamisole and closantel. We report the type and infection of GINs in Ordos, with the aim to help the prevention and control of GINs. Based on the results of the questionnaire survey and GIN resistance test, we found several reasons for the anthelmintic resistance of GINs, consequently providing new ideas for controlling the occurrence of anthelmintic resistance.

Assessing Goats' Fecal Avoidance Using Image Analysis-Based Monitoring

The recent advances in sensor technologies and data analysis could improve our capacity to acquire long-term and individual dataset on animal behavior. In livestock management, this is particularly interesting when behavioral data could be linked to production performances, physiological or genetical information, with the objective of improving animal health and welfare management. In this study, we proposed a framework, based on computer vision and deep learning, to automatically estimate animal location within pasture and discuss the relationship with the risk of gastrointestinal nematode (GIN) infection. We illustrated our framework for the monitoring of goats allowed to graze an experimental plot, where feces containing GIN infective larvae were previously dropped in delimited areas. Four animals were monitored, during two grazing weeks on the same pasture (week 1 from April 12 to 19, 2021 and week 2, from June 28 to July 5, 2021). Using the monitoring framework, different components of animal behavior were analyzed, and the relationship with the risk of GIN infection was explored. First, in average, 87.95% of the goats were detected, the detected individuals were identified with an average sensitivity of 94.9%, and an average precision of 94.8%. Second, the monitoring of the ability of the animal to avoid infected feces on pasture showed an important temporal and individual variability. Interestingly, the avoidance behavior of 3 animals increased during the second grazing week (Wilcoxon rank sum, p-value &amp;lt; 0.05), and the level of increase was correlated with the level of infection during week 1 (Pearson's correlation coefficient = 0.9). The relationship between the time spent on GIN-infested areas and the level of infection was also studied, but no clear relationship was found. In conclusion, due to the low number of studied animals, biological results should be interpreted with caution; nevertheless, the framework provided here is a new relevant tool to explore the relationship between ruminant behavior and GIN parasitism in experimental studies.

Variants Within Genes EDIL3 and ADGRB3 are Associated With Divergent Fecal Egg Counts in Katahdin Sheep at Weaning.

Gastrointestinal nematodes (GIN) pose a severe threat to sheep production worldwide. Anthelmintic drug resistance coupled with growing concern regarding potential environmental effects of drug use have demonstrated the necessity of implementing other methods of GIN control. The aim of this study was to test for genetic variants associated with resistance or susceptibility to GIN in Katahdin sheep to improve the current understanding of the genetic mechanisms responsible for host response to GIN. Linear regression and case-control genome-wide association studies were conducted with high-density genotype data and cube-root transformed weaning fecal egg counts (tFEC) of 583 Katahdin sheep. The case-control GWAS identified two significant SNPs (P-values 1.49e-08 to 1.01e-08) within introns of the gene adhesion G protein-coupled receptor B3 (ADGRB3) associated with lower fecal egg counts. With linear regression, four significant SNPs (P-values 7.82e-08 to 3.34e-08) were identified within the first intron of the gene EGF-like repeats and discoidin domains 3 (EDIL3). These identified SNPs were in very high linkage disequilibrium (r 2 of 0.996–1), and animals with alternate homozygous genotypes had significantly higher median weaning tFEC phenotypes compared to all other genotypes. Significant SNPs were queried through public databases to identify putative transcription factor binding site (TFBS) and potential lncRNA differences between reference and alternate alleles. Changes in TFBS were predicted at two SNPs, and one significant SNP was found to be within a predicted lncRNA sequence with greater than 90% similarity to a known lncRNA in the bovine genome. The gene EDIL3 has been described in other species for its roles in the inhibition and resolution of inflammation. Potential changes of EDIL3 expression mediated through lncRNA expression and/or transcription factor binding may impact the overall immune response and reduce the ability of Katahdin sheep to control GIN infection. This study lays the foundation for further research of EDIL3 and ADGRB3 towards understanding genetic mechanisms of susceptibility to GIN, and suggests these SNPs may contribute to genetic strategies for improving parasite resistance traits in sheep.

Variation in response of Mubende, Boer and Boer x Mubende goat breeds to gastrointestinal nematode infection under field conditions in Uganda

Variation in response of Mubende, Boer and Boer x Mubende goat breeds to gastrointestinal nematode infection under field conditions in Uganda

Determining the efficacy of Red Cell® in combination with anthelmintics against gastrointestinal nematode parasitism in sheep and goats

Small ruminants are highly susceptible to infection with gastrointestinal nematodes (GIN), particularly Haemonchus contortus, a blood-feeder that can cause severe anemia and death losses in untreated animals. Anecdotal evidence has suggested that short-term treatment of heavily infected animals with Red Cell®, a vitamin and mineral supplement, in combination with anthelmintic drugs may reduce morbidity and mortality. The objective of this study was to determine the effect of Red Cell® on GIN infection in small ruminants. Four experiments were conducted and comprised of a two-week trial using mixed-sex naturally parasitized hair sheep lambs or intact male Spanish goat kids housed on pasture. The treatments consisted of 30 mL Red Cell® with or without albendazole (Exp. 1, lambs), levamisole (Exp. 2, lambs and Exp. 3, goats), or moxidectin (Exp. 4, goats), the anthelmintic alone, or water only (control). Fecal and blood samples were collected from individual animals on Day 0, 7, and 14. Fecal egg counts and blood packed cell volume analyses were conducted. Overall, though anthelmintic treatments did influence FEC and PCV, there was no effect of Red Cell® treatment.

Genetic Parameter Analysis of Indicator Traits of Gastrointestinal Nematode Infection in Sheep Breeds

This study was undertaken with an aim to analyse the indicator traits of gastrointestinal nematode infection viz., FAMACHA score, FEC and PCV and to estimate the correlation among these traits. This study was carried out in a total of 100 sheep, 50 of each Kilakarsal and Vembur sheep breeds. The data were analysed using a mixed model least-squares analysis for fitting constants and the relationship among traits were estimated. The overall least-squares mean for FAMACHA scores were 2.88±1.144 and 2.9±0.151; FEC were 792±121.448 and 767.0±100.576; PCV were 26.38±0.199 and 26.14±0.189, in Kilakarsal and Vembur sheep, respectively. The Pearson Correlations study revealed that there is highly significant (P&lt;0.01) positive correlation between FAMACHA scores and FEC in Kilakarsal (0.835**) and Vembur (0.863**) sheep, But PCV is highly significantly negatively correlated with FAMACHA scores (Kilakarsal: -0.843**and Vembur: -0.883**) and FEC (Kilakarsal: - 0.874** and Vembur: -0.924**). According to Spearman’s rho correlations analysis, FAMACHA scores (Kilakarsal: -0.802** and Vembur: -0.893**) and FEC (Kilakarsal: -0.811** and Vembur: -0.920**) had negative associations with PCV which are highly significant (p&lt;0.01) and the FAMACHA scores exhibited a highly significant positive association with FEC with values of 0.957** and 0.954** in Kilakarsal and Vembur sheep, respectively. It is concluded that the FAMACHA score, FEC, PCV are the best indicator traits for gastrointestinal nematode infection, especially Haemonchus contortus is major disease causing pathogen. The correlation analysis of indicator traits will help in selection of genetically resistant animals against gastrointestinal infection.

A Review of the Impact of Climate Change on the Epidemiology of Gastrointestinal Nematode Infections in Small Ruminants and Wildlife in Tropical Conditions.

Climate change is causing detrimental changes in living organisms, including pathogens. This review aimed to determine how climate change has impacted livestock system management, and consequently, what factors influenced the gastrointestinal nematodes epidemiology in small ruminants under tropical conditions. The latter is orientated to find out the possible solutions responding to climate change adverse effects. Climate factors that affect the patterns of transmission of gastrointestinal parasites of domesticated ruminants are reviewed. Climate change has modified the behavior of several animal species, including parasites. For this reason, new control methods are required for controlling parasitic infections in livestock animals. After a pertinent literature analysis, conclusions and perspectives of control are given.

Investigating parasite dynamics of migratory ungulates for sustaining healthy populations: Application to critically-endangered saiga antelopes Saiga tatarica

Contact between wild and domestic ungulates is increasing across rangelands, enabling disease co-transmission. Disease management is difficult given uncertainties in complex system behavior, limited empirical data, and logistical obstacles to interventions. We studied gastrointestinal nematode (GIN) transmission in a rangeland shared by both livestock and the critically-endangered migratory saiga antelope, Saiga tatarica, in order to model infection dynamics under current and plausible future scenarios of increasing livestock numbers, climate change and anti-helminth treatments. Our model was parameterised for trichostrongylid GIN – a cause of mortality and morbidity in ungulates globally - using data on observed faecal nematode egg output and host numbers and distribution. Results showed that seasonal saiga migration leads to asymmetry in parasite transmission, with the majority of GIN acquired by saigas in their autumn and winter range through prior pasture contamination from livestock. Consequently, reducing parasite burdens in livestock early in the season in these areas could disproportionately reduce cross-transmission to saigas. Early-season GIN suppression in livestock in the saiga's spring and summer range was predicted to have weaker effect on parasite transmission to saigas but reduces infections during the calving period, potentially increasing population health and resilience at this critical time. Optimally timed treatments could offset the effects of increasing livestock numbers on GIN infection pressure, while climate warming had only marginal impacts on GIN transmission under all scenarios. Our findings could support better understanding and mitigation of factors affecting saiga health and rural livelihoods. Our approach is transferable to other systems, particularly those with migratory hosts.

Endoparasitism in commercial quails around Umuahia, Abia State, Nigeria

The enteric parasites of quails in Umuahia were investigated to determine their prevalence. Three hundred faecal samples, collected on voiding, were screened, with two hundred and five of them from deep litter and ninety-five from cages. Faecal analysis was conducted using the flotation and Mcmaster techniques. The overall prevalence of helminth infection was 167(55.6%) while that for coccidian oocysts was 89(29.6%). The prevalence of the nematode parasites detected in this study includes Ascaridia spp. 63(21.0%), Heterakis spp. -41(13.6%), Capillaria spp. -33(11.0%), Syngamus spp. 20(6.6%) and Strongyloides spp. 10(3.3%). The age-wise prevalence of gastrointestinal nematodes in young and adult quails was 41(42.2%) and 126(62.0%) respectively. Also, 33(34.0%) and 56(27.5%) young and adult quails were infected with coccidian oocysts, respectively. Enteric nematode parasites found in the cage managed and deep litter managed quails were 31(32.6%) and 136(66.3%) respectively. Also, 63(30.7%) and 26(27.3%) deep litter and cage managed quails respectively were infected with coccidian oocytes. It was concluded that gastrointestinal nematode and coccidian infections of quails are common in quails in Umuahia area regardless of the management system. This necessitates appropriate sustainable control measures to enhance a profitable poultry enterprise.