Induction Factor Research Articles

Genotoxic hazard and oxidative stress induced by wastewater irrigated soil with special reference to pesticides and heavy metal pollution

Due to enhancement of industrial growth and urbanization, soil contamination is increasing prominently. Therefore, it is important to examine possible adverse effects of industrial waste. Soil samples were might to be polluted with several heavy-metals and pesticides. Gas chromatographic results showed occurrence of high-level of organochlorine and organophosphate pesticides in studied soil samples. Genotoxicity of soil extracts was assessed using environmental-risk assessment models. Soil samples were extracted in hexane and dichloromethane solvents and were evaluated for genotoxic potential by prokaryotic (Ames test, plasmid nicking assay and E. coli K-12 DNA repair defective mutants) and eukaryotic (Allium cepa root chromosomal aberration and Vigna radiata seed-germination test) bioassays. Strain TA98 was found the most susceptible among soil extracts. The mutagenicity of hexane soil extract from wastewater irrigation was found to be higher than that of DCM samples in terms of mutagenic index, mutagenic potential, and induction factor for Ames strains. The damage in DNA repair defective mutants of hexane extracts were found higher compared to DCM extracts at dose of 20 μl/ml of culture. Survival in polA, lexA and recA mutants were 39%, 47% and 55% while treated with hexane extract. Allium cepa test, mitotic index was decreased in dose-dependent way and various kinds of chromosomal aberrations were found. Vigna radiata seeds germination and other parameters were also affected when treated with wastewater irrigated (WWI) soil. Oxidative stress in V. radiata roots were also showed under CLS microscope. Genotoxicity of WWI soil extract was also confirmed by plasmid nicking test. Our study provides possible explanation for the assessment of potential health and environmental hazards of the industrial region.

The effect of matrix stiffness on the chondrogenic differentiation of mesenchymal stem cells.

Articular cartilage is one of the most important weight-bearing components in human body, thus the chondrogenesis of stem cells is reactive to many intracellular and extracellular mechanical signals. As a unique physical cue, matrix stiffness plays an integral role in commitment of stem cell fate. However, when examining the downstream effects of matrix stiffness, most studies used different soluble factors to assist physical inducing process, which may mask the chondrogenic effects of matrix stiffness. Here we fabricated polyacrylamide (PAAm) hydrogels with gradient stiffness to unravel the role of matrix stiffness in chondrogenic process of mesenchymal stem cells (MSCs), with or without TGF-β3 as induction factor. The results showed that with micromass culture mimicking relatively high cell density in vivo, the chondrogenic differentiation of MSCs can be promoted by soft substrates (about 0.5kPa) independently with assembled cytoskeleton. Further analysis indicated that addition of TGF-β3 generally increased expression level of cartilage-related markers and masked the stiffness-derived expression pattern of hypertrophic markers. These results demonstrate how mechanical cues experienced in developmental context regulate commitment of stem cell fate and have significant impact on the design of tissue regeneration materials.

Recent progress in the manipulation of biochemical and biophysical cues for engineering functional tissues.

Tissue engineering (TE) is currently considered a cutting-edge discipline that offers the potential for developing treatments for health conditions that negatively affect the quality of life. This interdisciplinary field typically involves the combination of cells, scaffolds, and appropriate induction factors for the regeneration and repair of damaged tissue. Cell fate decisions, such as survival, proliferation, or differentiation, critically depend on various biochemical and biophysical factors provided by the extracellular environment during developmental, physiological, and pathological processes. Therefore, understanding the mechanisms of action of these factors is critical to accurately mimic the complex architecture of the extracellular environment of living tissues and improve the efficiency of TE approaches. In this review, we recapitulate the effects that biochemical and biophysical induction factors have on various aspects of cell fate. While the role of biochemical factors, such as growth factors, small molecules, extracellular matrix (ECM) components, and cytokines, has been extensively studied in the context of TE applications, it is only recently that we have begun to understand the effects of biophysical signals such as surface topography, mechanical, and electrical signals. These biophysical cues could provide a more robust set of stimuli to manipulate cell signaling pathways during the formation of the engineered tissue. Furthermore, the simultaneous application of different types of signals appears to elicit synergistic responses that are likely to improve functional outcomes, which could help translate results into successful clinical therapies in the future.

Experimental investigation of the effect of blade solidity on micro-scale and low tip-speed ratio wind turbines

It is well-established that micro-scale wind turbines require high blade solidity in order to overtake friction torque of all mechanical parts and starts operating. Therefore, multi-bladed micro-scale rotors with a low design tip-speed ratio λ are advocated. However, no consensual blade solidity is admitted by the scientific community at low Reynolds number and low tip-speed ratio because the reliability of the airfoil data, used in the blade element momentum theory, is questionable. The vast majority of the open literature has focused on the number of blades rather than varying blade chord length to increase the solidity. This experimental study carried out in a wind tunnel serves two purposes: to examine blade solidity effect on the power Cp and torque coefficients Cτ vs. tip-speed ratio curves at a fixed number of blades and to investigate its effects on the velocity distributions using stereoscopic particle image velocimetry (SPIV) for three tip-speed ratio λ=0.5, λ=1 and λ=1.4. Six 200mm diameter runners with 8 blades and various blade solidity from σ=1.5 to σ=0.5 were designed at λ=1 without using airfoil data. The results emphasise that the maximum power coefficient increases with blade solidity up to a maximum value Cp,max=0.29 reached for σ=1.25. High-solidity rotors have a very low cut-in wind speed V0=3.8 m s−1 and their torque coefficient Cτ decreases drastically and linearly while increasing the tip-speed ratio λ. These specificities could be of particular interest for energy harvesting of low speed air flow in order to power low-energy appliances. However, for low-solidity rotors, the Cτ vs. λ curves present a similar trend than the lift coefficient vs. angle of attack polar plots of isolated airfoil which is characterised by a significant drop in Cτ illustrating stall effect. An increase in blade solidity postpones and attenuates the stall effects due to greater mutual blade interactions. The SPIV recordings reveal that for high-solidity rotors the magnitude and radial profiles of axial and tangential induction factors and the flow deflection were close to the design settings. Moreover, the analysis exhibits that an increase in the blade solidity and tip-speed ratio leads to higher axial and tangential induction factors. The investigation of the wake highlights that the aerodynamic torque generated by a wind turbine is not produced in a same way as changing the blade solidity or the tip-speed ratio. To conclude, the best compromise between the maximum power coefficient, the cut-in wind speed, the mass of filament and the stability of the wake is achieved for the rotor with a blade solidity of σ=1.25.

Study of three wake control strategies for power maximization of offshore wind farms with different layouts

The wake regulation by cooperative yaw control, axis induction control, or their combination can significantly improve the total power output of wind farms. However, a comprehensive study of the three control strategies is still lacking, which hinders their engineering applications. To this end, the optimized yaw angles, induction factors, and total power promotion are systematically compared for the three control strategies on both a regularly arranged wind farm and an irregularly arranged realistic offshore wind farm. The results indicate that all the three strategies work similarly to achieve a net power gain by enhancing the power output of the downstream turbines while slightly downgrading the upstream turbines. For the regularly arranged wind farm, very notable power promotions are obtained in the aligned direction, and all the three control strategies show similar performance, but the promotions almost disappear with a small misalignment. In contrast, power promotion is less sensitive to the wind direction for the irregularly arranged wind farm. The promotion of the yaw control is much more predominate than that of the induction control, and the combined control only slightly outperforms the single yaw control in the realistic wind farm. In the prevailing wind direction, the total power of the realistic wind farm can be increased by 2.1% by the combined control strategy under the wind speed of 6 m/s.

Increasing power coefficient of small wind turbine over a wide tip speed range by determining proper design tip speed ratio and number of blades

This paper proposes a design strategy based on an understanding of basic rotor aerodynamics principles to achieve aerodynamically efficient small wind turbine rotor by determining proper design tip speed ratio (TSR) and number of blades. In the design and performance analyses of rotors, the blade element momentum (BEM) method was employed in conjunction with the Viterna-Corrigan post-stall model and Buhl’s wake state model. BEM simulations use the Re number dependent airfoil aerodynamic coefficients derived through Xfoil panel code. Reduced design tip speed ratio increased rotor solidity and hence blade Reynolds number. The width of the optimum TSR range, on the other hand, was found to decrease when the design TSR was less than a certain threshold. At high tip speeds, rotors with optimum design TSR were able to maintain an almost constant and near-ideal axial induction factor, resulting widest optimum TSR range for high aerodynamic efficiency. Increasing number of blades ( B) increased CP,max, but further increase in B lowered rotor efficiency due to reduced blade Re number. Based on CFD results, 5-blade SD7032 rotor with a design TSR of 4 achieved 12.3% higher CP,max compared to 0.9 m diameter 3-blade experimental reference rotor. Maximum achievable power coefficient of infinitely many-bladed ideal rotor with design TSR of 4 is 0.559, while CP,max of 5-blade SD7032 rotor is 0.492.

PENGARUH KECEPATAN PUTAR, KEDALAMAN PEMOTONGAN DAN LAJU PEMAKANAN TERHADAP GETARAN ARAH RADIAL PADA MESIN BUBUT CNC

Vibration in machine tools can be caused by many factors, one of which is the induction factor caused by cutting parameters. In this experiment, measurements of radial vibration were carried out due to spindle rotation speed, depth of cutting, and feed rate. The experiment was carried out on a Gildemeister NEF CNC lathe with S45C material using an ISO 6 Insert R16 tip ZCC-CT. Each factor has three levels and the experimental design uses a full factorial. Measurements were made using an accelerometer and the results were analyzed using ANOVA on Minitab 19. From the measurement results, it was found that the main factor causing vibration in the radial direction is the spindle rotation speed. The smallest vibration is obtained from a combination of spindle speed parameters of 795.77 rpm, depth of cut of 0.25 mm, and feed rate of 0.3 mm/rot. Keywords : vibration, radial direction, ANOVA

Resveratrol Synergistically Promotes BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells.

Background Mesenchymal stem cells (MSCs) differentiate into osteocytes, adipocytes, and chondrocytes. Resveratrol and bone morphogenetic protein 9 (BMP9) are known osteogenic induction factors of MSCs, but the effect of both resveratrol and BMP9 on osteogenesis is unknown. Herein, we explored whether resveratrol cooperates with BMP9 to improve osteogenic induction. Methods The osteogenic induction of resveratrol and BMP9 on C3H10T1/2 cells was evaluated by detecting the staining and activity of the early osteogenic marker alkaline phosphatase (ALP). In addition, the late osteogenic effect was measured by the mRNA and protein levels of osteogenic markers, such as osteopontin (OPN) and osteocalcin (OCN). To assess the bone formation function of resveratrol plus BMP9 in vivo, we transplanted BMP9-infected C3H10T1/2 cells into nude mice followed by intragastric injection of resveratrol. Western blot (WB) analysis was utilized to elucidate the mechanism of resveratrol plus BMP9. Results Resveratrol not only enhanced osteogenic induction alone but also improved BMP9-induced ALP at 3, 5, and 7 d postinduction. Both the early osteogenic markers (ALP, Runx2, and SP7) and the late osteogenic markers (OPN and OCN) were significantly increased when resveratrol was combined with BMP9. The fetal limb explant culture further verified these results. The in vivo bone formation experiment, which involved transplanting BMP9-overexpressing C3H10T1/2 cells into nude mice, also confirmed that resveratrol synergistically enhanced the BMP9-induced bone formation function. Resveratrol phosphorylated adenosine monophosphate- (AMP-) activated protein kinase (AMPK) and stimulated autophagy, but these effects were abolished by inhibiting AMPK and Beclin1 using an inhibitor or siRNA. Conclusions Resveratrol combined with BMP9 significantly improves the osteogenic induction of C3H10T1/2 cells by activating AMPK and autophagy.

Binary colloidal crystals (BCCs) modulate the retina-related gene expression of hBMSCs – A preliminary study

Surface topography-induced lineage commitment of human bone marrow stem cells (hBMSCs) has been reported. However, this effect on hBMSC differentiation toward retinal pigment epithelium (RPE)-like cells has not been explored. Herein, a family of cell culture substrates called binary colloidal crystals (BCCs) was used to stimulate hBMSCs into RPE-like cells without induction factors. Two BCCs, named SiPS (silica (Si)/polystyrene (PS)) and SiPSC (Si/carboxylated PS), having similar surface topographies but different surface chemistry was used for cell culture. The result showed that cell proliferation was no difference between the two BCCs and tissue culture polystyrene (TCPS) control. However, the cell attachment, spreading area, and aspect ratio between surfaces were significantly changed. For example, cells displayed more elongated on SiPS (aspect ratio ~7.0) than those on SiPSC and TCPS (~2.0). The size of focal adhesions on SiPSC (~1.6 µm2) was smaller than that on the TCPS (~2.5 µm2). qPCR results showed that hBMSCs expressed higher RPE progenitor genes (i.e., MITF and PAX6) on day 15, and mature RPE genes (i.e., CRALBP and RPE65) on day 30 on SiPS than TCPS. On the other hand, the expression of optical vesicle or neuroretina genes (i.e., MITF and VSX2) was upregulated on day 15 on SiPSC compared to the TCPS. This study reveals that hBMSCs could be modulated into different cell subtypes depending on the BCC combinations. This study shows the potential of BCCs in controlling stem cell differentiation.

Reversion of neuronal differentiation induced in human adipose-derived stem cells

Adipose-derived mesenchymal stem cells are a great alternative to other types of stem cells obtained from other tissues, since they are found in large numbers and are easy to obtain by liposuction and do not have ethical connotations. These cells can differentiate into neuronal lineage using induction media, although the efficacy of these media is determined by their composition. In previous studies, we have demonstrated the differentiation of human adipose-derived mesenchymal stem cells to neuronal lineage by using three induction media, Neu1, Neu2 and Neu3, each one showing a series of neuronal markers in the treated cells. In the present study, a further step is taken, since once a neuronal differentiation is obtained with these three induction media, they are removed from the medium and both morphology and neuronal markers and the ability to maintain neurosphere formation are analyzed. The results obtained show that when these induction media are withdrawn in the three cases, both the neuronal morphological characteristics and the neuronal markers are lost at different times depending on the medium used. In the case of neurospheres, the ability to maintain this shape is also lost when they are not in contact with neuronal induction factors. Therefore, although the neuronal differentiation mechanism is very promising in this type of cells, it is necessary to carry out more studies to elucidate an induction medium that allows cells differentiated into neurons to maintain neuronal characteristics over time without the need to continue applying neuronal differentiation factors.

Study on infection behavior and characteristics of poplar wood dyed by Lasiodiplodia theobromae.

The technology of dyeing wood by microorganisms is a kind of pollution-free and sustainable wood dyeing technology. To achieve fast and rich dyeing of Lasiodiplodia theobromae on the surface of poplar wood, tyrosinase and tricyclazole were used as induction factors in this experiment. The results showed that L. theobromae had a better induction effect in the cross-section of poplar wood and induced with tricyclazole. The surface color of poplar ranged from light yellow dyeing to gray and brown, the chromatic aberration of the cross-section of wood was above 44.5 NBS, and the infected area was over 50%, while the dyed parts of radial and tangential sections of wood were only on the surface of the wood after 30 days of infection. The induced infection of L. theobromae on poplar wood had little effect on the chemical components of poplar and had good colorfastness to washing and light. Therefore, microbial dyeing of wood showed a beneficial application prospect in the field of wood dyeing.

Picking Path Planning Method of Dual Rollers Type Safflower Picking Robot Based on Improved Ant Colony Algorithm

Aiming at the problem of automatic path planning for the whole safflower bulbs during the operation of safflower picking robots, an improved ant colony algorithm (ACA) was proposed to plan the three-dimensional path of the safflower picking points. The shortest time and distance were taken as the overall goal of path planning to comprehensively improve the working efficiency of safflower picking robots. First, in order to shorten time, the angle induction factor was introduced to reduce the angle rotation of the end-effector. Second, in order to shorten the length of the picking path, the picking track was optimized. Finally, the design of the secondary path optimization reduced the number of picking points, which not only shortened the length of the picking path, but also shortened the picking time. The simulation results show that the path planned by the improved ACA was reduced by three picking points, shortening the total length by 74.32%, and reducing the picking time by 0.957 s. The simulation results verify the feasibility of the improved ACA for safflower picking path planning, which provides theoretical reference and technical support for the picking path planning of dual roller safflower picking robots.

Stepwise Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells in Collagen Sponges under Different Microenvironments.

Biomimetic microenvironments are important for controlling stem cell functions. In this study, different microenvironmental conditions were investigated for the stepwise control of proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The hMSCs were first cultured in collagen porous sponges and then embedded with or without collagen hydrogels for continual culture under different culture conditions. The different influences of collagen sponges, collagen hydrogels, and induction factors were investigated. The collagen sponges were beneficial for cell proliferation. The collagen sponges also promoted chondrogenic differentiation during culture in chondrogenic medium, which was superior to the effect of collagen sponges embedded with hydrogels without loading of induction factors. However, collagen sponges embedded with collagen hydrogels and loaded with induction factors had the same level of promotive effect on chondrogenic differentiation as collagen sponges during in vitro culture in chondrogenic medium and showed the highest promotive effect during in vivo subcutaneous implantation. The combination of collagen sponges with collagen hydrogels and induction factors could provide a platform for cell proliferation at an early stage and subsequent chondrogenic differentiation at a late stage. The results provide useful information for the chondrogenic differentiation of stem cells and cartilage tissue engineering.

Higher Concentration of Ascorbic Acid as a Sole Induction Factor for Osteogenesis on MC3T3-E1 Cell Model

This research aimed to investigate the potential of ascorbic acid (Asc) to act independently as an osteogenic induction factor in a murine pre-osteoblast (MC3T3-E1) model. MC3T3-E1 cells were seeded in culture wells and hydroxyapatite scaffold for two-dimensional and three-dimensional analyses respectively. Cell morphology, viability, osteoblast differentiation, and mineralisation potentials of MC3T3-E1 cells were compared between induction of standard (50 µg/mL) and doubled (100 µg/mL) Asc concentrations in growth media. Cells with fibroblast-like morphology became confluent earlier on day 6 in the standard group compared to the doubled group on day 9. Cell viability and differentiation potential were significantly increased in the doubled group (p < 0.01). Mineralisation occurred in the doubled group after 15 days of seeding but no mineralisation was seen in the standard group. Findings were similar in 3D analysis whereby mineralized nodules were seen only in the doubled group. The relative expression of collagen 1(α) and osteocalcin genes were increased in the doubled group than the standard group. Doubling Asc concentration in a growth medium to 100 µg/mL can induce viability, differentiation, and mineralisation of MC3T3-E1 cells. Thus, higher concentration of ascorbic acid can potentially be used as the sole induction factor in osteogenic medium.

Effects of omega-3 fatty acid supplementation during chronic maternal hypoxiaon behavioral disorders in male rat offspring: the role of Trk family and oxidative stress.

Maternal hypoxia due to a lack of blood flow and insufficient oxygen supply in the brain leads to behavioral disorders in adult offspring. Fish oil includes docosahexaenoic acid (DHA), a significant component of membrane phospholipids of nerve cells, which improved cognition, and memory. Trk family receptors are activated by hypoxic induction factor (HIF), and are involved in the neurotrophin's protective effects at the cellular level. Here we studied the biochemical, and molecular mechanisms of the protective effect of fish oil during the chronic maternal hypoxia model on behavioral responses in male rat offspring. Pregnant female rats were randomly divided into 4 experimental groups: 1) ctr; Control rats were pregnant 2) Hyp; Pregnant female rats received hypoxia from 6 to 15th day of pregnancy, with 10% oxygen intensity, and 90% nitrogen; 3) FO; Pregnant female rats received fish oil (F8020 1ml / day, for ten consecutive days Orally), and 4) FO / Hyp; Pregnant female rats received hypoxia plus fish oil in the same manner. Behavioral parameters were evaluated in 28-day-old male offspring. HIF-1α, TrkB, and P75 gene expression were measured in the offspring's brain. Maternal hypoxia impaired memory performance, and locomotor activity in offspring. Besides, Trk family gene expression, and oxidative stress indicators showed a significant increase in the offspring's brain exposed to maternal hypoxia compared to the control group. Overall, fish oil improved behavioral parameters by inhibiting oxidative stress, and the expression of Trk family receptors.

Improved Prediction of Aerodynamic Loss Propagation as Entropy Rise in Wind Turbines Using Multifidelity Analysis

Several physics-based enhancements are embedded in a low-fidelity general unducted rotor design analysis tool developed, py_BEM, including the local Reynolds number effect, rotational corrections to airfoil polar, stall delay model, high induction factor correction, polar at large angle of attack, exergetic efficiency calculation and momentum-based loss. A wind turbine rotor is analyzed in high fidelity designed from py_BEM using a 3D blade generator. It is a design derived from the NREL Phase VI rotor. Three design variations are analyzed using steady 3D CFD solutions to demonstrate the effect of geometry on aerodynamics. S809 and NACA 2420 airfoil properties are used for calculating the aerodynamic loading. Momentum, vorticity and energy transport are explained in depth and connected to entropy production as a measure of performance loss. KE dissipation downstream of the rotor is shown to be a significant contributor of entropy rise. Wake analysis demonstrates mixing with the free stream flow, which begins after 3 diameters downstream of the rotor and extends to about 25 diameters until the decay is very small. Vorticity dynamics is investigated using a boundary vorticity flux technique to demonstrate the relationship between streamwise vorticity and lift generated in boundary layers. Drag components are accounted as well. It is demonstrated using rothalpy that shaft power is not only torque multiplied by rotational velocity but a viscous power loss term must also be included. A multifidelity analysis of wind turbine aerodynamics is demonstrated by capturing flow physics at several levels.

Whole-cell bacterial biosensor for volatile detection from Pectobacterium-infected potatoes enables early identification of potato tuber soft rot disease

Half of the harvested food is lost due to rots caused by microorganisms. Plants emit various volatile organic compounds (VOCs) into their surrounding environment, and the VOC profiles of healthy crops are altered upon infection. In this study, a whole-cell bacterial biosensor was used for the early identification of potato tuber soft rot disease caused by the pectinolytic bacteria Pectobacterium in potato tubers. The detection is based on monitoring the luminescent responses of the bacteria panel to changes in the VOC profile following inoculation. First, gas chromatography-mass spectrometry (GC-MS) was used to specify the differences between the VOC patterns of the inoculated and non-inoculated potato tubers during early infection. Five VOCs were identified, 1-octanol, phenylethyl alcohol, 2-ethyl hexanol, nonanal, and 1-octen-3-ol. Then, the infection was detected by the bioreporter bacterial panel, firstly measured in a 96-well plate in solution, and then also tested in potato plugs and validated in whole tubers. Examination of the bacterial panel responses showed an extensive cytotoxic effect over the testing period, as seen by the elevated induction factor (IF) values in the bacterial strain TV1061 after exposure to both potato plugs and whole tubers. Moreover, quorum sensing influences were also observed by the elevated IF values in the bacterial strain K802NR. The developed whole-cell biosensor system based on bacterial detection will allow more efficient crop management during postharvest, storage, and transport of crops, to reduce food losses.

Immune reactions as induction factors and correction of the pathology

On clinical models of infectious, somatic, malignant, and other diseases, the ability of immune responses to induce pathological processes due to competition, “pathogenicity”, hypersensitivity, and insufficiency has been shown. We are talking about the competitiveness of antibacterial and antiviral immunity, when the deployment of cellular and humoral immune responses weaken both defense mechanisms, competition of allergy and immune deficiency, which aggravates and modifies it, and antiviral immunity often induces a number of pathological conditions of the body. This is due to the inability of antiviral antibodies to completely inactivate the antireceptor structures of virions, interact with them without changing their function, and even stimulate a viral infection, induce autoimmune reactions. The protective system of interferons, which often cause side reactions, is also faulty. In the body, immunopathological reactions develop, manifested by immediate and delayed hypersensitivity, which have alternative properties – protective and damaging and autoimmune reactions, mainly with a negative effect. The mechanisms of the processes are discussed in detail in the review. The “inferiority” of immune reactions, respectively, cellular, humoral, phagocytic links, incomplete consistency of anticancer immunity, virus-induced immunodeficiencies are comprehensively covered. Such “alternativeness” of the considered immune reactions, at the same time, turns out to be a necessary condition for eliminating the emerging disorders of the organism’s reactivity. These include specificity and non-specificity, stimulation and suppression, variability and integration of immune responses. At the same time, immunoregulatory reactions are individual and interrelated, develop regionally and systemically and provide degradation of exogenous and endogenous objects and immune activation. All these aspects are discussed in detail in the review.

Maximization of the Power Production of an Offshore Wind Farm

Operating wind turbines together as a wind farm can be more advantageous and economical. As a result, onshore and offshore wind farms are being built at a rapid pace around the world. Wake effects, which have a negative impact on overall wind farm electricity generation, are one of the key concerns in wind farms. This work concentrates on the maximization of power output from wind farms by ameliorating the wake effect. This work introduces a dynamic wind farm controller that adjusts turbines’ yaw angles or axial induction factors following the flow field conditions to maximize the overall power output of the wind farm. This research examines a real-life offshore wind farm in South Korea and the wind farm controller is evaluated in Wind Farm Simulator (WFSim), a control-oriented dynamic wind farm model environment built by Delft University of Technology. The main contribution of this work includes investigating the impact of wind farm control methods on the power production of a wind farm model that simulates a real-life wind farm.

Daily Consumption of Lemon and Ginger Herbal Infusion Caused Tumor Regression and Activation of the Immune System in a Mouse Model of Breast Cancer.

The Mediterranean diet includes the consumption of various fruits and vegetables. Lemon and ginger are highly popular in Mediterranean cuisine. The current study aims to evaluate both anticancer and immunomodulatory activities of lemon and ginger combination. The antiproliferative activities of the combination were tested against different cancer cell lines using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The degree of apoptosis induction and vascular endothelial growth factor expression were detected using ELISA. Balb/C mice were inoculated with the EMT6/P breast cancer cells and received combination water extract orally for 14 days. The effect of the water extract on splenocytes proliferation was measured using the mitogen proliferation assay. Macrophage function was evaluated using the nitro blue tetrazolium assay and pinocytosis was assessed using the neutral red method. Gas chromatography coupled to the tandem mass spectrometry was used to determine the composition of the combination. The lemon and ginger combination showed significant apoptosis induction and angiogenesis suppression effects. Fifty percent of the mice taking this combination did not develop tumors with a percentage of tumor reduction of 32.8%. This combination showed a potent effect in stimulating pinocytosis. Alpha-pinene and α-terpineol were detected in high percentages in the combination water extract. The lemon and ginger combination represents promising options to develop anticancer infusions for augmenting conventional anticancer therapies. Further testing is required to understand the exact molecular mechanisms of this combination.