Mounting evidence show possible association between air pollution and reproductive potential of human. Our study assessed the association between air pollution and markers of ovarian reserve in women who visited a fertility center. 2,276 women who visited a single fertility center were included for study and their ovarian reserve were assessed. Average concentration of pollutant where enrolled women lived were measured to see the correlation between air pollution and ovarian reserve. This study included 2,276 women aged between 20 and 50 who visited a single fertility center from Jan. 2016 to Sep. 2018. Ovarian reserve was assessed by measuring serum FSH and AMH on menstrual day 3. Hourly concentrations of fine particulate matter (PM10), NO2, CO, SO2, and O3 measured at 269 air quality monitoring sites across country was used. Based on daily mean concentrations of each air pollutant, average concentration was computed for within 1, 3, 6 and 12 months. Ovarian reserve indicators were observed to expected AMH ratio, FSH > 20IU/mL and AMH < 0.5ng/mL. We assessed the association between concentration of six air pollutants and ovarian reserve indicators adjusting for individual socio-demographic, lifestyle, and spatiotemporal factors. Mean age of women was 36.6 ± 4.2. Majority (n=1431, 62.9%) was working at the time of ovarian reserve test and 51 (2.2%) had past smoking history. Mean FSH and AMH were 9.2 ± 6.4 IU/mL and 3.3 ± 3.1 ng/mL, respectively. Prevalence of high FSH was 3.4% (n=69) and decreased AMH was 10.3% (n=235). AMH ratio was 0.8 ± 0.7 on average. In linear regression models, an IQR-increase of 1 month-average of PM10 concentration (adjusted ß coefficient=-0.06, 95%confidenceinterval:-0.11, 0.00) and NO2 concentration (-0.07, 95%CI:-0.12 -0.01) was associated with lower AMH ratio. Except O3, there were generally negative association between air pollutants concentration and AMH ratio which did not reach statistical significance. In the models for binary outcomes, OR of high FSH was 2.00 (95% CI: 1.08 3.72) per IQR-increase of 12 month-average concentration of SO2. On the other hand, OR of high FSH was 0.33 (95% CI: 0.13 0.82) with IQR-increase of O3 concentration within 1 month. Higher exposure to PM10 and SO2 was associated with low ovarian reserve in infertile patients, highlighting the importance of the air pollution to infertility.