Abstract Background Serum copper, zinc and selenium, are commonly ordered for assessment of nutrient status or investigation of deficiency or overload. Review of reference intervals for these analytes is challenging as most laboratories do not have the resources to do a de novo reference interval study and many literature references are not recent. The National Health and Nutrition Examination Survey (NHANES) survey presents a unique opportunity to access a comprehensive database that includes biochemical parameters and health information in order to review reference intervals regularly, especially for more esoteric tests such as trace elements. In this study, copper, zinc and selenium reference intervals were calculated using NHANES data. Methods NHANES data for individuals 18 years of age and older was obtained from three releases between 2012-2016. Copper, selenium and zinc 95% confidence intervals were evaluated by age, sex and body mass index (BMI) partitions using quantile regression. All calculations were performed in R using the quantreg package. Results The calculated reference intervals for serum copper, zinc and selenium are shown in Table 1. Serum copper had the largest changes observed between sexes, with females having a much wider reference interval on the high end. Oral contraceptive use is known to increase serum copper levels which likely explains this trend. BMI over 30 also increased the serum copper reference interval, more clearly in women than in men. Zinc reference intervals were more aligned between women and men, with slightly higher intervals observed in men. BMI did not have much effect in either sex. For selenium, men had slightly higher reference intervals than women. Conclusions NHANES data provides a comprehensive data set for reference interval evaluation, especially for more esoteric analytes such as trace elements.