Content Index Research Articles

Feature Extraction of Flow Sediment Content of Hydropower Unit Based on Voiceprint Signal

The hydropower turbine parts running in the sand-bearing flow will experience surface wear, leading to a decline in the hydropower unit’s stability, mechanical performance, and efficiency. A voiceprint signal-based method is proposed for extracting the flow sediment content feature of the hydropower unit. Firstly, the operating voiceprint information of the hydropower unit is obtained, and the signal is decomposed by the Ensemble Empirical Mode Decomposition (EEMD) algorithm, and a series of intrinsic mode functions (IMFs) are obtained. Combined with correlation analysis, more sensitive IMF components are extracted and input into a convolutional neural network (CNN) for training, and the multi-dimensional output of the fully connected layer of CNN is used as the feature vector. The k-means clustering algorithm is used to calculate the eigenvector clustering center of the hydropower unit with a clean flow state and a high sediment content state, and the characteristic index of the hydropower unit sediment content is constructed based on the Euclidean distance method. We define this characteristic index as SI, and the change in the SI value can reflect the degree of sediment content in the flow of the unit. A higher SI value indicates a lower sediment content, while a lower SI value suggests a higher sediment content. Combined with the sediment voiceprint data of the test bench, when the water flow changed from clear water to high sediment flow (1.492 × 105 mg/L), the SI value decreased from 1 to 0.06, and when the water flow with high sediment content returned to clear water, the SI value returned to 1. The experiment proves the effectiveness of the method. The extracted feature index can be used to detect the flow sediment content of the hydropower unit and give early warning in time, so as to improve the maintenance level of the hydropower unit.

Correlations between a Friabilin Content Indicator and Selected Physicochemical and Mechanical Properties of Wheat Grain for Processing Suitability Assessment

A new approach to determining the friabilin content of wheat grain was proposed. Electropherograms were taken, and the intensity of the friabilin bands was compared in the analyzed wheat cultivars and the cv. Chinese Spring. The friabilin content indicator was calculated in the grain of 17 common wheat cultivars, which differed mostly in their crude protein content and hardness index (HI). The basic properties of the kernels were measured in each wheat cultivar, and the correlations between the measured parameters and the friabilin content indicator were determined. In the analyzed wheat cultivars, the friabilin content indicator ranged from around 0.21 to around 0.77. This indicator was significantly correlated with the kernel length, thickness, mass, vitreousness, HI, and rupture force. The strongest correlation was observed between the friabilin content indicator and kernel length. An increase in the mean kernel length from around 5.4 mm to around 8.0 mm decreased the friabilin content indicator by approximately 51%. After the mean kernel length had been calculated in a given wheat cultivar, a certain value of the friabilin content indicator could be ascribed to this cultivar, and the energy consumption during grain grinding or milling could be partly predicted. In the group of analyzed wheat cultivars, the process of grain grinding would be the most energy-intensive in the cvs. Ceres, SMH200, and SMH214 and the least energy-intensive in the cvs. Chinese Spring, Julius, and Askalon.

DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status

The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in the group of intact immature animals both in fresh FSTT and after exposure to cryomedium or cryopreservation, while the indexes of DNA fragmentation and ROS content had the lowest values. It was found that an increase in the DNA fragmentation rate occurred in parallel with a decrease in the values of antioxidant activity and membrane integrity. The spermatogenenic epithelium after cryopreservation differed between the groups in a relative number of cells with pathologically changed nuclei and the frequency of exfoliation of epithelial cells into the tubule cavity namely, there was a tendency to an increase in the damaging effects in the series, “Immature → Sexually mature → Autoimmune arthritis”. The obtained data can be taken into account in the development of low-temperature preservation protocols using cryotechnologies, which will ensure the maintenance of the morphological and functional characteristics of FSTT depending on sexual maturity and immunological status.

Extraction of Phycocyanin and Chlorophyll from Spirulina by “Green Methods”

Phycocyanin is a pigment–protein complex from the group of phycobiliproteins obtained from Spirulina (Arthrospira platensis), with possibilities for various applications in food and pharmaceutical technologies. It is a natural colorant for food and cosmetic products. This study aimed to investigate the effect of ultrasonic and microwave extraction conditions on antioxidant activity (AOA), chlorophyll content, and the content and purity index of phycocyanin in aqueous and alcoholic extracts of Spirulina (Arthrospira platensis). For this purpose, ultrasonic extraction with water or ethanol was performed at 20 °C, 30 °C, and 40 °C for 1, 2, and 3 h at an ultrasonic frequency of 36 kHz, 40 kHz, and 45 kHz. Microwave water extraction was performed for 60 s, 120 s, and 180 s. For each of the obtained samples, three parallel measurements of antioxidant activity were made by DPPH and FRAP methods, and chlorophyll content and phycocyanin yield and purity index were determined spectrophotometrically. Ultrasonic extraction resulted in a higher yield and purity index of phycocyanin compared to microwave extraction. The highest yield of 14.88 mg g−1 with a purity index of 1.60 was achieved at a temperature of 40 °C for one hour and an ultrasonic wave frequency of 40 kHz. A relatively low yield of 4.21 mg g−1, but with a purity index of 2.67, was obtained at a temperature of 30 °C, a time of two hours, and an ultrasonic frequency of 40 kHz. Chlorophyll b content at 20 °C, for two hours and ultrasonic frequency 40 kHz was 1.400 mg g−1. The study proposes ultrasonic extraction as a green method to obtain phycocyanin of varying purity index that may be used for food, cosmetic, or biomedical purposes.

Synchronous Retrieval of Wheat Cab and LAI from UAV Remote Sensing: Application of the Optimized Estimation Inversion Framework

Chlorophyll a and b content (Cab) and leaf area index (LAI) are two key parameters of crops, and their quantitative inversions are important for growth monitoring and the field management of wheat. However, due to the close correlation between the spectral signals of these two parameters and the effects of soil and atmospheric conditions, as well as modeling errors, synchronous retrieval of LAI and Cab from remote sensing data is still a challenging task. In a previous study, we introduced the optimal estimation theory and established the inversion framework by coupling the PROSAIL (PROSPECT + SAIL) model with the unified linearized vector radiative transfer model (UNL-VRTM). The framework fully utilizes the simulated radiance spectra for synchronous retrieval of Cab and LAI at the UAV observation scale and has good convergence and self-consistency. In this study, based on this inversion framework, synchronized retrieval of Cab and LAI was carried out by real wheat UAV observation data and validated with the ground-measured data. By comparing with the empirical statistical model constructed by the PROSAIL model and coupled model, least squares support vector machine (LSSVM), and random forest (RF), the proposed method has the highest accuracy of Cab and LAI estimated from UAV multispectral data (for Cab, R2 = 0.835, RMSE = 14.357; for LAI, R2 = 0.892, RMSE = 0.564). Our proposed method enables the fast and efficient estimation of Cab and LAI in multispectral data without prior measurements and training.

Enhancing stress resilience in rice (Oryza sativa L.) through profiling early-stage morpho-physiological and molecular responses to multiple abiotic stress tolerance.

Under changing climatic conditions, crop plants are more adversely affected by a combination of various abiotic stresses than by a single abiotic stress. Therefore, it is essential to identify potential donors to multiple abiotic stresses for developing climate-resilient crop varieties. Hence, the present study was undertaken with 41 germplasm accessions comprising native landraces of Tamil Nadu, Prerelease lines and cultivars were screened independently for drought, salinity, and submergence at the seedling stage during Kharif and Rabi 2022-2023. Stress was imposed separately for these three abiotic stresses on 21-day-old seedlings and was maintained for 10 days. The studied genotypes showed a significant reduction in plant biomass (PB), Relative Growth Index (RGI), relative water content (RWC), leaf photosynthesis, chlorophyll fluorescence, and Chlorophyll Concentration Index (CCI) under drought followed by salinity and submergence. Stress-tolerant indices for drought, salinity, and submergence revealed significant variation for plant biomass. Furthermore, a set of 30 SSR markers linked to drought, salinity, and submergence QTLs has been used to characterize 41 rice germplasm accessions. Our analysis suggests a significantly high polymorphism, with 28 polymorphic markers having a 93.40% in 76 loci. The mean values of polymorphic information content (PIC), heterozygosity index (HI), marker index (MI), and resolving power (RP) were 0.369, 0.433, 1.140, and 2.877, respectively. Jaccard clustering grouped all the genotypes into two major and six subclusters. According to STRUCTURE analysis, all genotypes were grouped into two major clusters, which are concurrent with a very broad genetic base (K = 2). Statistically significant marker-trait associations for biomass were observed for five polymorphic markers, viz., RM211, RM212 (drought), RM10694 (salinity), RM219, and RM21 (submergence). Similarly, significant markers for relative shoot length were observed for RM551 (drought), RM10694 (salinity), and ART5 (submergence). Notably, the genotypes Mattaikar, Varigarudan samba, Arupatham samba, and APD19002 were identified as potential donors for multiple abiotic stress tolerance. Thus, identifying the genetic potential of germplasm could be useful for enhancing stress resilience in rice.

Phenolic content and lipid quality of nuts of baru fruits submitted to pest management and stored at different temperatures

Baru is a fruit produced only once a year which requires adequate planning of seasonal maintenance to maintain the quality of its nut for consumption. This study aimed to evaluate the effect of hydrothermal treatment on pest control and temperature and storage time on baru fruits on the phenolic content and the lipidic quality of the nuts. We stored the fruits at ambient temperature (29°C) and climatized at 18°C. We evaluated them at 0, 60, 120, and 180 days for insect damage, water content, total phenols, tannins, antioxidant activity, lipid profile, acidity, peroxide and iodine indices, and oxidative stability by Rancimat. The integrated management was efficient in pest control, and nuts extracted from fruits preserved the bioactive compounds independently of storage temperature and presented chemical characteristics acceptable for consumption. The fatty acids found in higher proportions in baru nuts were oleic (50%) and linoleic acids (27%) and remained preserved over time. Thus, it promotes the supply of oilseeds to the market with consistency and quality between crops, avoiding unnecessary losses during storage.

Relationship between GIX, SIDX, and ROTI ionospheric indices and GNSS precise positioning results under geomagnetic storms

Ionospheric indices give information about ionospheric perturbations, which may cause absorption, diffraction, refraction, and scattering of radio signals, including those from global navigation satellite systems (GNSS). Therefore, there may be a relationship between index values and GNSS positioning results. A thorough understanding of ionospheric indices and their relationship to positioning results can help monitor and forecast the reliability and accuracy of GNSS positioning and support the precision and safety of life applications. In this study, we present the relationship between three indices: Gradient Ionosphere indeX (GIX), Sudden Ionospheric Disturbance indeX (SIDX), and Rate of Total electron content Index (ROTI) in relation to precise positioning results. We used two approaches: precise point positioning (PPP) and relative positioning for long baselines. We focus on GNSS stations located in Europe for two selected geomagnetic storms: March 17, 2015, and May 22, 2015. Our results show that in the case of PPP, positioning degradation occurred mainly at high latitudes and was mostly caused by rapid small-scale changes in ionospheric electron content represented by SIDX and ROTI. We also showed a significant correlation between cycle slips of GNSS signals and ROTI (0.88). The most significant degradations for relative positioning for low and medium latitudes were associated with large spatial gradients reflected by the GIX.

Comparative Evaluation of Nano Urea Versus Conventional Urea for Nitrogen Management in Rainfed Sunflower (Helianthus annuus L.) Cultivation in Acid Sandy Loam Soils of Assam.

A field experiment was conducted during rabi, 2021-22 at the experimental plot of the Department of Agronomy, Biswanath College of Agriculture (BNCA), Assam Agricultural University (AAU), Biswanath Chariali, Assam having geographic coordinates 26°43'30'' N and 93°08'08'' E to assess nitrogen management in Sunflower (Helianthus annuus L.) with conventional and nano urea under rainfed farming situations in the North Bank Plain Zone of Assam. The experiment confined to 10 treatments in Randomized Block Design (RBD) viz., T1: Control (No Nitrogen), T2: Soil application of 50% N, T3: Soil application of 100% N (RD), T4: 3 foliar applications of 0.6% nano urea, T5: Soil application of 50% N + 2 foliar application of 1% conventional urea, T6: Soil application of 50% N + 2 foliar application of 2% conventional urea, T7: Soil application of 50% N + 2 foliar application of 3% conventional urea, T8: Soil application of 50% N + 2 foliar application of 0.2% nano urea, T9: Soil application of 50% N + 2 foliar application of 0.4% nano urea, T10: Soil application of 50% N + 2 foliar application of 0.6% nano urea. Results revealed that there was no significant effect on the seedling emergence percentage due to different nitrogen management treatments. The highest plant height (84 cm) and maximum number of leaves (33) were found in T10. T9 showed the maximum values of Relative Leaf Water Content (RLWC) (71%), leaf area index (LAI) (3.2 and 4.2 at 60 and 90 DAS), chlorophyll content index (30 DAS) and head diameter (16.70 cm). For most of the growth and phenological parameters, T9 and T10 were at par with each other. T1 (no nitrogen) was the earliest to complete its life cycle which is reflected in 50% heading (65 days) and days to maturity (97 days). Dry matter accumulation varied at different time intervals, initially, it was highest in T3 but later T9 and T10 showed the maximum values. In terms of seed yield, test weight and chaffy grain percentage (%), T9 showed the best results: (1505 kg/ha), (54 g/1000 seeds) and (20%) respectively, followed by T10. Whereas the highest stover yield was recorded in T10. The highest Benefit to Cost ratio (B:C ratio of 1.60) was obtained in the T9.

Analysis of secondary metabolites through callus cell suspension culture of black cumin (Nigella sativa L.) with PGR combination on MS media

This study aims to resolve the effect of using ZPT picloram and BAP and a combination of MS media on cell suspension culture and secondary metabolite compounds contained in black cumin (Nigella sativa L.) callus. The enquiry was performed at the Tissue Culture Laboratory, Faculty of Agriculture and Phytochemical Laboratory, Faculty of Pharmacy, Universitas Sumatera Utara, from February 2023 to July 2023. This study used a Totally Randomized Design (RAL) method amid 2 treatment elements and each treatment had 3 replications. The first treatment factor was ZPT concentration in MS medium (Z): Z1 (Picloram 0 ppm + BAP 1 ppm), Z2 (Picloram 1 ppm + BAP 1 ppm), Z3 (Picloram 1 ppm + BAP 2 ppm), Z4 (Picloram 2 ppm + BAP 2 ppm). The second factor is the composition of the MS (M) media: M1 (½ MS) and M2 (MS). Parameters observed were callus morphology, growth index, SCV (Settled Cell Volume), PCV (Peacked Cell Volume) and analysis of secondary metabolite content. The outcomes exposed that the arrangement of MS media and the PGR concentration of picloram and BAP had no expressive impact on all parameters. Z4 and M2 concentrations provided the most optimal growth.

Salt stress influences the proliferation of Fusarium solani and enhances the severity of wilt disease in potato

Soil salinity has emerged as a critical abiotic stress in potato production, whereas wilt disease, caused by Fusarium solani, is the significant biotic stress. An experiment was performed to decipher the occurrence of wilt incidence by F. solani FJ1 under the influence of salinity in both in vitroand pot culture conditions. High salt concentration negatively influenced root and shoot development in the variety “Kufri Jyoti” but positively affected the mycelial growth and sporulation behaviours of F. solani FJ1. There was abundant whitish mycelial growth with enhanced biomass and high sporulation (microconidia production) in F. solani FJ1 cultured on salt-supplemented media. Moreover, under high salinity conditions (EC 2–8 dS m−1), severe wilting and rotting of vascular bundles were observed in plants artificially inoculated with F. solani FJ1. The mortality rate of potato plants was significantly higher under individual and combined stresses as compared to control. The wilt index of individual and combined stressed plants was also substantially higher compared to the control. Additionally, compared to the control, there was a significant decrease in total chlorophyll content and membrane stability index of the leaves under combined stress. However, the total phenols were increased under stress conditions. The total sugar content of potato plants decreased in infected plants, but increased when exposed to salt stress or a combination of salt stress and pathogen infection. F. solani infection also increased the activity of peroxidase (POX) and decreased the activity of phenylalanine ammonia-lyase (PAL) and catalase (CAT). These results suggest that Fusarium wilt and dry rot will be a more severe disease for potato cultivation in saline soils.

An Improved Stochastic Model for the Geodetic GNSS Receivers Under Ionospheric Scintillation at Low Latitudes

AbstractThe receiver tracking error stochastic (RTES) model can improve GNSS precise point positioning (PPP) performance under ionospheric scintillation. However, it relies on scintillation products derived from ionospheric scintillation monitoring receivers (ISMRs), which means the RTES model cannot be used for abundant geodetic GNSS receivers. In this study, we propose an improved RTES, referred to as Impr_RTES model, to mitigate scintillation effects on geodetic GNSS receivers at low latitudes, where severe scintillation frequently occurs. In the Impr_RTES model, the tracking error variances at the output of code delay locked loop are calculated by using the index S4c, and these of phase locked loop are modeled by using the rate of total electron content index (ROTI) and S4c. Both S4c and ROTI can be derived from geodetic GNSS receivers. The performance of the Impr_RTES model is validated by using the data sets from ISMR and geodetic receivers, respectively. Using one month of GPS data collected at HNLW station installed with ISMR in Hainan of China from 1 to 28 February in 2023, statistical results indicate that the PPP solution based on Impr_RTES model can improve the positioning accuracy by approximately 22.6%, 23.8%, and 30.2% in the east, north, and up directions, respectively, over the elevation angle stochastic (EAS) model. Meanwhile, the positioning performance of Impr_RTES PPP is comparable to that of RTES PPP. For the GPS data from geodetic receivers, experimental results suggest that compared with EAS, the Impr_RTES model can obviously mitigate scintillation effects on PPP.

Development of Instant Beverage Powder Based on Sacha Inchi

Sacha inchi (Plukenetia volubilis L.) seed oil gains an industrial’s attention although other parts of the seed also have economical and nutritional potentials. This study was aimed to produce instant beverage powder based on sacha inchi by formula optimization and investigation the effect of drumdrying conditions on its physicochemical and sensorial properties. Results from a mixture design showed that the most suitable formula composed of 64% sacha inchi seed extraction, 20% sweet corn and 16% instant oat, in addition a penalty analysis revealed that the most preferable sugar adding level was 8% (w/w). In term of drum-drying condition, rotation speed and maltodextrin concentration strongly infl uenced moisture content, ΔE* and water solubility index (WSI) while they had no effect on rehydration ratio. The physicochemical and sensorial properties of the instant beverage powder obtained from this study were similar to those of commercial product except for WSI. This study demonstrates that, besides seed oil, sacha inchi seed can be valorized and utilized as a novel food ingredient for an instant beverage powder production.

NaCl Kaynaklı Tuz Stresinin Lotus corniculatus L. cv. 'Leo'nun Çimlenmesi ve İlk Fide Büyümesi Üzerindeki Etkisi

Lotus corniculatus L. is one of the agronomically and economically important perennial legume forage species with moderately salt-tolerant. It is well known that even the cultivars of the same species in plants have different responses in salinity. However, studies on the salt response of L. corniculatus and its cultivars, which are more advantageous than other forage crops such as white clover (Trifolium repens) and alfalfa (Medicago sativa L.), in the use of marginal agricultural lands affected by abiotic stress factors such as salinity, are limited. Under salt stress, the most crucial phases of the plant life cycle that are directly related to the survival of the plant are seed germination, growth, and vigour. Therefore, this study was carried out to determine the germination and growth responses of L. corniculatus cultivar 'Leo', which is known to have higher tannin content than other cultivars, under NaCl-derived salt stress in vitro. For this purpose, L. corniculatus seeds were cultured in MS (Murashige and Skoog/Gamborg) medium containing 0, 40, and 80 mM NaCl for 14 days. Seed germination percentage, mean germination time, germination rate index, shoot-root length, root to shoot length ratio, shoot-root fresh dry weight, shoot-root dry matter, the ratio of root to shoot dry matter, shoot-root water content and seedling vigour index parameters were measured. According to the results of the research, the germination percentage did not change in the applied NaCl treatments, but the germination rate decreased. However, shoot length decreased and root length increased. Although there was no statistically significant change in shoot and root fresh-dry weight, both decreased in 80 mM NaCl treatment. The shoot and root dry matter increased and the water content decreased. Also, the seedling viability index decreased. In 40 mM NaCl treatment, on the other hand, there was an increase in shoot fresh-dry weight, dry matter ratio and seedling viability index with the positive effect of low dose. Within the scope of this study, comprehensive information was presented for L. corniculatus (cultivar 'Leo'), an important forage plant, in terms of germination and seedling growth under salt stress.

Rapid and low-cost screening for single and combined effects of drought and heat stress on the morpho-physiological traits of African eggplant (Solanum aethiopicum) germplasm.

Drought and heat are two stresses that often occur together and may pose significant risks to crops in future climates. However, the combined effects of these two stressors have received less attention than single-stressor investigations. This study used a rapid and straightforward phenotyping method to quantify the variation in 128 African eggplant genotype responses to drought, heat, and the combined effects of heat and drought at the seedling stage. The study found that the morphophysiological traits varied significantly among the 128 eggplants, highlighting variation in response to abiotic stresses. Broad-sense heritability was high (> 0.60) for chlorophyll content, plant biomass and performance index, electrolyte leakage, and total leaf area. Positive and significant relationships existed between biomass and photosynthetic parameters, but a negative association existed between electrolyte leakage and morpho-physiological traits. The plants underwent more significant stress when drought and heat stress were imposed concurrently than under single stresses, with the impact of drought on the plants being more detrimental than heat. There were antagonistic effects on the morphophysiology of the eggplants when heat and drought stress were applied together. Resilient genotypes such as RV100503, RV100501, JAMBA, LOC3, RV100164, RV100169, LOC 3, RV100483, GH5155, RV100430, GH1087, GH1087*, RV100388, RV100387, RV100391 maintained high relative water content, low electrolyte leakage, high Fv/Fm ratio and performance index, and increased biomass production under abiotic stress conditions. The antagonistic interactions between heat and drought observed here may be retained or enhanced during several stress combinations typical of plants' environments and must be factored into efforts to develop climate change-resilient crops. This paper demonstrates improvised climate chambers for high throughput, reliable, rapid, and cost-effective screening for heat and drought and combined stress tolerance in plants.

The effect of ballast moisture content and fouling index on railway track settlement

A primary function of railway ballast is to maintain vertical track geometry, however over time it becomes contaminated with fines which reduce its ability to free-drain. Then, in the presence of moisture, the individual particles have greater scope for rearrangement thus leading to increased track settlement. This behaviour has received limited studied from a differential settlement viewpoint despite track geometry being the dominant metric used for scheduling railway maintenance. Therefore this paper presents a novel numerical modelling approach capable of simulating the effect of ballast fouling and moisture content on the evolution of track geometry. First the model is presented, which uses a 2.5D finite element approach with perfectly matched layers to simulate static and dynamic stress fields from trains. Non-linear track-soil effects are considered and loading is applied using a multi-body vehicle with train-track coupling. Differential track settlement is calculated in an iterative manner, being updated after every train passage. It is used to perform four analyses, the first of which is to investigate the effect of ballast fouling considering dry conditions. Next the effect of moisture of fines is studied. Then, the effect of earthwork stiffness and train speed is analysed. It is shown that elevated levels of ballast fouling lead to faster deterioration in track geometry, particularly in the presence of moisture. When the ballast is heavily fouled and the moisture of fines is high, rapid deterioration occurs. In contrast, when the ballast is kept dry, the increase in degradation rate for fouled ballast is limited. Higher train speeds and lower earthwork stiffness’s make this effect more pronounced.

The amelioration of salt stress-induced damage in fenugreek through the application of cold plasma and melatonin

Nowadays, it is increasingly crucial to combine innovative approaches with established methods to enhance plant tolerance and maximize the production of beneficial compounds. With this aim in view, a study was carried out to investigate how different melatonin concentrations (0, 30, and 60 ppm), cold plasma treatment (at 3000 and 4000 V), and varying exposure durations (0, 1, 2, and 4 min) affect the physiological and biochemical attributes of fenugreek plants, as well as the levels of diosgenin under salinity stress. This study revealed that the application of 3000 V cold plasma for 2 min with 60 ppm melatonin by establishing cellular redox homeostasis in salinity-treated fenugreek plants, effectively prevented the destruction of pigments and reduced the electrolyte leakage index of malondialdehyde content. The utilization of these two elicitors has the potential to trigger multiple pathways, including the enzymatic and non-enzymatic antioxidants biosynthesis, and abscisic acid-dependent pathways. This activation results in an enhanced production of abscisic acid, auxin, and endogenous melatonin, along with the regulation of signal transduction pathways. Surprisingly, applying these two treatments increased the expression of SQS, CAS, SSR, BGL, SEP, SMT, and diosgenin content by 13, 22.5, 21.6, 19, 15.4, 12, and 6 times respectively. The findings highlight the intricate interplay between these treatments and the positive impact of their combined application, opening up avenues for further research and practical applications in improving plant tolerance to environmental stresses.

Isolation and Characterization of Starch from Different Potato Cultivars Grown in Croatia

Starch is a polysaccharide that is widely used in food and other industries; therefore, due to its great potential, it is attempted to be maximally isolated from various foods rich in starch. Commonly, potatoes are used for starch production due to the relatively high starch content in tubers, and the process itself is complex and includes several steps. The aim of this study was to isolate and characterize starch from eight potato varieties. First, the basic chemical composition of the potato samples was determined, and then the isolation was carried out under laboratory conditions. The isolated starch was air dried, then ground and sieved through a 400 µm sieve. The basic chemical composition, amylose content, starch color, swelling capacity and solubility index, clarity of starch pastes, texture of starch gels and thermo-physical properties (gelatinization and retrogradation) were determined in the obtained starch samples. The results showed that the SL 13–25 potato variety had the lowest starch content, while the Stilleto variety had the highest starch content. The content of protein, fat, ash and crude fiber was relatively low in all of the isolated starches, indicating their high purity. Also, the difference in the color of the isolated starches was difficult to see. The highest amylose content had starch from Saprodi, while the lowest was observed in starch from SL 13–25. Starch from the cv. Dartiest had the highest, while starch from the cv. Sereno had the lowest gel strength. The starch of the cv. Dartiest also had the highest clarity value. The retrogradation transition temperatures and enthalpies were lower compared to the gelatinization temperatures and enthalpies. With the increasing temperature, both the swelling capacity and the solubility index of all the samples increased. According to the obtained differences, this study confirmed the significant influence and role of different cultivars on starch characteristics.

Characteristics of Hozat apricot seed oil as a new source of oilseed

There has been an increase in apricot consumption due to its high bioactive and fiber content. However, the seed, inside part of the fruit, is considered as a food waste. In this study, potential usage of apricot seeds (bitter and sweet), that were cultivated in Hozat region- Tunceli, as an alternative oil source was evaluated. No comprehensive research has been undertaken to assess the seeds as an oil source before. In assessing its suitability for consumption, proximate composition of seed and physicochemical properties of the seeds’ oil had been determined. The proximate composition included the content of protein, ash, moisture, oil and the acidity as pH, in addition; iodine value, refractive index, nutritional quality, total phenolic content, antioxidant content, fatty acid composition and rheological properties of the oil were determined. Also, the effect of variety on them was examined.Overall, oil content was found to be the major component of the seed, thus the seed is good enough to be used as an oil source. Five different fatty acids were identified in the oil, that produced from the seed. These were palmitic, heptadecanoic, oleic, linoleic and linolenic acid. The oil had a refractive index between 1.4617 and 1.4628, iodine value between 100.47 and 102.11 gms, total phenolic content between 0.83 and 1.29 mg GAE g−1 oil, antioxidant activity between 11.21 and 11.60 mg TE g−1 oil for DPPH assay and 7.92–8.20 mg TE g−1 oil for ABTS assay, and apparent viscosity of 0.04 Pa.s. Moreover, viscous flow tests showed that the oil has a Newtonian flow behavior, and the nutritional indexes, that include unsaturated, atherogenicity, thrombogenicity and hypocholesterolemic/ hypercholesterolemic ratio, indicated that the oil is good enough for consumption as an oil source. The variety was found to have a significant effect on total phenolic content, antioxidant activity and refractive index of the oil, that was produced from apricot seed.

Estimation of Geotechnical Parameters for Coal Exploration from Quasi-3D Electrical Resistivity Measurements

Geotechnical parameters are crucial for mine planning and operation at different stages of development. However, estimating these parameters requires a large number of boreholes and subsequent detailed analysis of the samples, making it a cumbersome exercise. Moreover, even after conducting these studies, it is not possible to cover the entire operational area. To address this issue, this study presents an indirect method of estimating geotechnical parameters through mathematical relations using resistivity data. The present study incorporated 2D and 3D subsurface imaging techniques for exploring coal reserves and analyzing geotechnical parameters that define subsurface soil properties. Electrical resistivity tomography (ERT) was utilized for data acquisition, employing a Dipole–dipole array with a multielectrode ABEM Terrameter LS instrument. Six parallel profiles were conducted, each 400 m in length, with an inter-electrode spacing of 10 m and a spacing of 50 m between profiles. These profiles were combined into a 3D dataset referred to as quasi-3D ERT. The inversion process for both 2D and 3D data was performed using the Res2dinv and Res3dinv programs, respectively. This study overcame the challenges of 2D resistivity sections by evaluating horizontal depth slices in the x-z plane from layers 1 to 10, reaching a depth of 81.2 m. The geotechnical parameters, including cohesion, friction angle, moisture content, and plastic index, were derived from the resistivity data. The ERT method proved to be cost-effective and efficient in determining soil properties over a large area compared with traditional laboratory analysis of borehole samples. Additionally, the variation of geotechnical parameters with resistivity values exhibited unique characteristics. The results from both the 2D and quasi-3D ERT were well correlated with the borehole data. Such studies are valuable for resource exploration and mine planning purposes.