Analysis of secondary metabolites through callus cell suspension culture of black cumin (Nigella sativa L.) with PGR combination on MS media

Abstract

This study aims to resolve the effect of using ZPT picloram and BAP and a combination of MS media on cell suspension culture and secondary metabolite compounds contained in black cumin (Nigella sativa L.) callus. The enquiry was performed at the Tissue Culture Laboratory, Faculty of Agriculture and Phytochemical Laboratory, Faculty of Pharmacy, Universitas Sumatera Utara, from February 2023 to July 2023. This study used a Totally Randomized Design (RAL) method amid 2 treatment elements and each treatment had 3 replications. The first treatment factor was ZPT concentration in MS medium (Z): Z1 (Picloram 0 ppm + BAP 1 ppm), Z2 (Picloram 1 ppm + BAP 1 ppm), Z3 (Picloram 1 ppm + BAP 2 ppm), Z4 (Picloram 2 ppm + BAP 2 ppm). The second factor is the composition of the MS (M) media: M1 (½ MS) and M2 (MS). Parameters observed were callus morphology, growth index, SCV (Settled Cell Volume), PCV (Peacked Cell Volume) and analysis of secondary metabolite content. The outcomes exposed that the arrangement of MS media and the PGR concentration of picloram and BAP had no expressive impact on all parameters. Z4 and M2 concentrations provided the most optimal growth.