The impact of conservation tillage practices on carbon sequestration has been of great interest in recent years. Changes in the soil organic carbon (SOC) as influenced by tillage, is more noticeable under long-term rather than short-term tillage practices. This experiment analyzed the organic carbon status of soils sampled at depth increments from 0 to 60 cm after 25 years of five tillage treatments in a silt loam soil. Zero tillage (ZT) treatment was compared to conventional tillage practices of mouldboard and chisel plow operations conducted either during the fall or spring season in a randomized complete block design with four replications. The SOC was calculated on depth and equivalent soil mass bases. Contrast analysis showed a significantly (5%) higher soil bulk density for zero versus fall and zero versus chisel tillage operations at 5–10 cm soil depth. The SOC concentration was dependent on the depth of tillage operation and followed the trend of higher SOC for zero, chisel, and mouldboard tillage at 0–5, 5–10, and 20–40 cm depth, respectively. There were more significant differences in the SOC storage when expressed on depth compared to an equivalent soil mass basis. SOC storage was significantly higher for ZT at the 0–5 cm soil depth compared to conventional tillage practices. Contrast analysis on an equivalent mass basis showed that SOC storage was significantly higher for spring tillage compared to fall tillage at 0–60 cm depth. In conclusion, ZT practices increased SOC concentration and storage compared to conventional tillage operations only for the surface layer but not for the entire soil profile.
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