The main target of this work is to realize the function of pre-oxidizing NO from diesel engine’s exhaust by using self-designed double-dielectric Non-thermal Plasma (NTP) reactor. The majorized discharge frequency and discharge peak to peak voltage (Vp-p) range for NTP reactor were obtained through air discharge test. The diesel engine test bench was established to observe the effect of NTP on the volume fraction of NO. The results showed that there were more active substances and fewer by-products in NTP reactor when discharge frequency was 9 kHz and Vp-p was between 9 kV and 23 kV; Exhaust flows had insignificant effect on the performance of NTP pre-oxidizing NO; The ability of NTP to pre-oxidize NO gradually weakened with the increase of engine load, and when the engine load were 0 % and 25 %, the ratio of NO/NO2 could reach 1. In such working conditions, SCR system could improve the conversion rate of the NOx at low-temperature zone through quick reaction combined with NTP.