Event Abstract Back to Event Effects and mechanisms of action of glufosinate, an organophosphate pesticide, on dopaminergic neurotransmition. Brenda Viviane-Ferreira-Nunes1*, I. M. Oliveira1, R. C. Cervantes1, M. Alfonso1, Rafael Duran1 and Lilian R. Faro1 1 Universidad de Vigo, Depto. Biologia Funcional y Ciencias de la Salud, Spain Objective:The objective of this work was to determine the effect of different concentrations of glufosinateammonium (GNA) and the mechanisms of action of this organophosphate, on the dopamine (DA) release in striatum of awake and free moving rats, using the technique of brain microdialysis.Methods:The GNA administration, as well as the collection of samples was made by brain microdialysis, neurochemistry technique that allows the access to the extracellular liquid of cerebral areas in awake and free moving animals. The experiments had been carried out in female Sprague-Dawleyrats (250-300g, n=5/group), kept in standardized room’s conditions. The determination and quantification of DA was made by liquid chromatography (HPLC) with electrochemical detection. The statistics analysis was made by ANOVA/Student-Newman-Keulstest. Significant differences: *P<0.05, **P<0.01, and ***P<0.001.Results:The intraestriataladministration of 1, 10 and 20mM of GNA, for 1 h, produced significant increases in extracellular DA levels (459.5±157.1%, 1.022 ±368.6%, and 3106.5 ±523%, respect to the basal, respectively). These increases may be due to exocytoticrelease and/or a change in the activity of DA transporter (DAT). To verify this hypothesis, we evaluate the effect of the infusion of GNA (10 mM) in a free Ca++medium or together with nomifensine(NOM), a DAT blocker. The infusion of Ringer without Ca++decreased the levels of DA to 41 ±18%, respect to the basal. When the GNA was infused together with the Ringer without Ca++, the levels of DA increased to 1000 ±175.6%, respect to basal levels, an increase statically similarto those observed when GNA was infused with the normal Ringer. The participation of the DAT in GNA-induced DA release was evaluated through the co-infusion of GNA (10mM) and NOM (20μM). Intrastriatalinfusion of NOM produced an increase of 1632 ±240% in DA levels and the NOM+GNA infusion increased DA levels to 1540 ±634.14% respect to basal levels.Conclusions:These finds suggest that intrastriatalinfusion of GNA increases the DA release in a concentration-dependent manner. This release occurs through a mechanism not dependent of calcium and dependent of the DAT.Financial Support. MAEC-AECID, Xuntade Galicia, Universidad de Vigo. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Neuronal Communication Citation: Viviane-Ferreira-Nunes B, Oliveira IM, Cervantes RC, Alfonso M, Duran R and Faro LR (2009). Effects and mechanisms of action of glufosinate, an organophosphate pesticide, on dopaminergic neurotransmition.. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.155 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Aug 2009; Published Online: 12 Aug 2009. * Correspondence: Brenda Viviane-Ferreira-Nunes, Universidad de Vigo, Depto. Biologia Funcional y Ciencias de la Salud, Vigo, Spain, brendaneuro@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Brenda Viviane-Ferreira-Nunes I. M Oliveira R. C Cervantes M. Alfonso Rafael Duran Lilian R Faro Google Brenda Viviane-Ferreira-Nunes I. M Oliveira R. C Cervantes M. Alfonso Rafael Duran Lilian R Faro Google Scholar Brenda Viviane-Ferreira-Nunes I. M Oliveira R. C Cervantes M. Alfonso Rafael Duran Lilian R Faro PubMed Brenda Viviane-Ferreira-Nunes I. M Oliveira R. C Cervantes M. Alfonso Rafael Duran Lilian R Faro Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.