Monitoring row centerlines during early growth stages is essential for effective production management. However, detection becomes more challenging due to weed interference and crop row intersection in images. This study proposed an enhanced Region of Interest (ROI)-based approach for detecting early-stage maize rows. It integrated a modified green vegetation index with a dual-threshold algorithm for background segmentation. The median filtering algorithm was also selected to effectively remove most noise points. Next, an improved ROI-based feature point extraction method was used to eliminate residual noises and extract feature points. Finally, the least square method was employed to fit the row centerlines. The detection accuracy of the proposed method was evaluated using the unmanned aerial vehicle (UAV) image data set containing both regular and intersecting crop rows. The average detection accuracy of the proposed approach was between 0.456° and 0.789° (the angle between the fitted centerline and the expert line), depending on whether crop rows were regular/intersecting. Compared to the Hough Transform (HT) algorithm, the results demonstrated that the proposed method achieved higher accuracy and robustness in detecting regular and intersecting crop rows. The proposed method in this study is helpful for refined agricultural management such as fertilization and irrigation. Additionally, it can detect the missing-seedling regions and replenish seedings in time to increase crop yields.