Micro-supercapacitors (MSCs) with outstanding flexibility and electrochemical performance are critical for portable and miniaturized electronics. This study utilizes the potential utilization of Boron-modified MoS2/MXene heterostructure for fabricating highly efficient and durable microsupercapacitors. The Boron incorporation and hybrid formation significantly enhance the electrochemical performance of 1 T MoS2 and MXene (Ti3C2Tx) by activating their basal plane. The boron modified MoS2/Ti3C2Tx(B-1 T MoS2/Ti3C2Tx_) demonstrates excellent energy storage performance with a capacitance of around 420 Fg−1 in two-electrode system. Laser scribed graphene (LSG), chosen for its superior wettability, serves as the current collector facilitating the development of these MSC based on B-1 T MoS2/Ti3C2Tx. The resulting MSC demonstrates a high areal capacitance of nearly 72.31 mF/cm2 and excellent stability over 10,000 cycles. Density functional theory (DFT) simulations support these findings, indicating that hybridization with Ti3C2Tx and B substitution enhances the conductivity of the 1 T-MoS2 system. The quantum capacitance of B-substituted hybrid system surpasses that of the pristine material, validating the experimental observations of enhanced charge storage performance.