Incorporation Of Silver Nanoparticles Research Articles

Fusion-Assisted Hydrothermal Synthesis of Technogenic-Waste-Derived Zeolites and Nanocomposites: Synthesis, Characterization, and Mercury (II) Adsorption.

This study presents the synthesis of zeolites derived from coal fly ash (CFA) using the fusion-assisted alkaline hydrothermal method. The zeolites were synthesized by combining CFA and NaOH at a molar ratio of 1:1.2 under fusion temperatures of 500, 600, and 700 °C. Subsequently, the obtained zeolites were subjected to further modifications through the incorporation of magnetic (Fe3O4) and silver (Ag0) nanoparticles (NPs). The Fe3O4 NPs were introduced through co-precipitation of Fe(NO3)2 and FeCl3 at a molar ratio of 1:1, followed by thermal curing at 120 °C. On the other hand, the Ag0 NPs were incorporated via ion exchange of Na+ with Ag+ and subsequent reduction using NaBH4. The synthesized porous materials exhibited the formation of zeolites, specifically analcime and sodalite, as confirmed by X-ray diffraction (XRD) analysis. Additionally, the presence of Fe3O4 and Ag0 NPs was also confirmed by XRD analysis. The elemental composition analysis of the synthesized nanocomposites further validated the successful formation of Fe3O4 and Ag0 NPs. Nitrogen porosimetric analysis revealed the formation of a microporous structure, with the BET surface area of the zeolites and nanocomposites ranging from 48.6 to 128.7 m2/g and pore sizes ranging from 0.6 to 4.8 nm. The porosimetric characteristics of the zeolites exhibited noticeable changes after the modification process, which can be attributed to the impregnation of Fe3O4 and Ag0 NPs. The findings of this research demonstrate the effectiveness of the fusion-assisted method in producing synthetic zeolites and nanocomposites derived from CFA. The resulting composites were evaluated for their potential application in the removal of mercury ions from aqueous solutions. Among the samples tested, the composite containing Ag0 NPs exhibited the highest adsorption capacity, reaching 107.4 mg of Hg2+ per gram of composite. The composites modified with Fe3O4 NPs and Ag/Fe3O4 nanocomposites displayed adsorption capacities of 68.4 mg/g and 71.4 mg/g, respectively.

Effect of exposure conditions on chemical properties of materials for surgical endodontic procedures.

This study investigated the role of aging and changes in environmental conditions on selected properties of a prototype radiopacified calcium silicate-based cement (TZ-base) with or without incorporation of silver nanoparticles or bioactive glass, and two commercial materials, Biodentine and intermediate restorative material. Materials were immersed in ultrapure water or fetal bovine serum for 28 days and were characterized with scanning electron microscopy and energy dispersive x-ray analysis. Immersion media were either replaced weekly or not replenished at all and were assessed for alkalinity and calcium release after 1, 7, 14, 21, and 28 days; antibacterial effect against 2-day monospecies biofilms; and cytotoxicity by the 3-(4,5 dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide assay after 1, 7, or 28 days. Alkalinity, calcium release, antibacterial activity, and cell cytotoxicity increased over time when the medium was not changed but decreased with medium replenishment. Immersion in fetal bovine serum resulted in lower alkalinity, less bactericidal properties, and lower cytotoxicity of prototype cements and Biodentine than did water immersion. Biodentine and 20% bioactive glass-containing cement had overall lower alkalinity, calcium release, and antibacterial activity than TZ-base, and Biodentine was less cytotoxic than TZ-base. In conclusion, exposure conditions and cement modifications significantly affected materials' leaching properties. Exposure conditions warrant consideration when evaluating cements' clinical properties.

Spectroscopic analysis of plant trace element incorporated silver nanoparticles synthesis from Datura metel l.

Here in we report, silver nanoparticles (Ag NPs) prepared from leaf extract of Datura metel L. via green synthesis method. Datura metel L. is an herbal medicinal plant of solanaceae family. The as - prepared Ag NPs characterized employing UV-Vis spectrometer, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDAX) techniques. The appearance of Surface Plasmon Resonance (SPR) peak at 415 nm suggested that creation of Ag NPs in UV-Vis spectrum. XRD pattern showed face centered cubic crystal structure of Ag NPs with organometallic complex phase. Peaks in FTIR spectrum reveal the existence of biomolecules. SEM image illustrate their clastic rock morphology and EDAX profile authenticate the company of Ag and plant trace element. The Cyclic voltammetry (CV), Choronopotentiometry, and Electrochemical impedance spectroscopy (EIS) analysis were performed as prepared Ag electrode. The specific capacitance of 267.59 F/g at 0.5 A/g and cyclic retention of 83.7% after charge-discharge 5000 cycles are obtained. Hence this material could be utilized as Supercapacitor energy storage devices.

Fabrication of antimicrobial colorimetric pad for meat packaging based on polyvinyl alcohol aerogel with the incorporation of anthocyanins and silver nanoparticles

AbstractA novel colorimetric pad with porous structure, good antimicrobial activity, and pH‐sensitivity has been developed based on polyvinyl alcohol (PVA), paper fibre, silver nanoparticles (AgNPs) and purple sweet potato anthocyanins (PSPA), aiming for the preservation and freshness indication of fresh meat. The effects of AgNPs content on the structure, physical properties and colour‐changing behaviour in response to pH of the pad were investigated. The results showed that AgNPs physically interacted with polymer matrix and improved the uniformity of the internal pore structure of the pad. The antibacterial capability, DPPH radical scavenging activity and surface hydrophobicity of the pad increased significantly with increasing AgNPs content. However, the pH sensitivity of the pad was maintained with the addition of AgNPs, and the colour change of pad A‐9 with pH was the most distinguishable when AgNPs content was the highest. When pad A‐6 was used in the packaging of minced pork, compared to pad A‐0 without the addition of AgNPs, it showed more significant effects in inhibiting microbial growth, reducing the production of volatile nitrogen compounds, improving the sensory quality of meat (p < 0.05), thereby extending the shelf life from 72 h to 120 h at 4°C. Moreover, A‐6 displayed a visible colour change in response to the spoilage of minced pork in real time. Therefore, the developed pad with dual functions had great potential to be applied as a novel active and intelligent packaging for fresh meat, which would contribute towards reducing food waste and ensuring food safety.

One-pot synthesis of CIS nano-particles and their EMI shielding studies

EMI Shielding behaviour of copper indium sulphide (CIS) nanoparticles synthesized by single-step method is presented. 1-Octadecene and oleic acid both play important role in the synthesis, however, presence of oleylamine leads to formation of phase pure CIS as confirmed by XRD. It is observed that incorporation of silver nanoparticles in 1:1 amount with respect to CIS, dramatically enhanced the shielding efficiency from about −3 dB to about −13.5 dB (average) of the CIS/PVA (0.5:1 w/w) composite in X-band. This is possibly the first study of CIS for EMI application.

An antibacterial and bioactive sponge incorporating Codium sp.-mediated biosynthesized silver nanoparticles for the management of high exudate wounds

The antibacterial sponges with interconnected porous structure, high biocompatibility and antibacterial activity are important for wound healing applications. In this work, Codium sp. — polysaccharide based sponges incorporating silver nanoparticles biosynthesized by macroalgal biomass were produced. The biosynthesized silver nanoparticles were successfully produced through chemical reduction mediated by residual macroalgal biomass. As the main findings, the nanoparticles presented a hydrodynamic diameter of 46.10 ± 0.22 nm and a zeta potential value of −18.20 ± 1.02 mV, which remained stable for 14 days. In turn, the porous sponges based on Codium sp. polysaccharide incorporating the silver nanoparticles were obtained by freeze-drying. The produced sponges presented a controlled swelling (≈2000 % stable during at least 35 h of incubation) and biodegradation profile (sponges lost about 80 % of its weight after 5 days). Further, the sponges demonstrated to be biocompatible in contact with fibroblast cells and able to avoid microorganism growth. Overall, the results suggested the promising properties of the sponges incorporating biosynthesized silver nanoparticles to act as antibacterial and bioactive wound dressing able to absorb the excess of wound exudate, promoting cell proliferation and impairing the occurrence of a skin infection. Such evidence reinforces the properties of macroalgal-derived products to be applied in the biomedical field and the use of macroalgal biomass for the biosynthesis of metallic nanoparticles.

Part B: Improvement of the Optical Properties of Cellulose Nanocrystals Reinforced Thermoplastic Starch Bio-Composite Films by Ex Situ Incorporation of Green Silver Nanoparticles from Chaetomorpha linum.

The study was used in the context of realigning novel low-cost materials for their better and improved optical properties. Emphasis was placed on the bio-nanocomposite approach for producing cellulose/starch/silver nanoparticle films. These polymeric films were produced using the solution casting technique followed by the thermal evaporation process. The structural model of the bio-composite films (CS:CL-CNC7:3-50%) was developed from our previous study. Subsequently, in order to improve the optical properties of bio-composite films, bio-nanocomposites were prepared by incorporating silver nanoparticles (AgNPs) ex situ at various concentrations (5-50% w/w). Characterization was conducted using UV-Visible (UV-Vis), Fourier Transform Infrared (FTIR), Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) to understand the structure-property relationships. The FTIR analysis indicated a reduction in the number of waves associated with the OH functional groups by adding AgNPs due to the formation of new hydrogen bonds between the bio-composite matrix and the CL-WE-AgNPs. Based on mathematical equations, the optical bandgap energy, the energy of Urbach, the edge of absorption (Ed), and the carbon clusters (N) were estimated for CS:CL-CNC and CS:CL-CNC-AgNPs (5-50%) nanocomposite films. Furthermore, the optical bandgap values were shifted to the lower photon energy from 3.12 to 2.58 eV by increasing the AgNPs content, which indicates the semi-conductor effect on the composite system. The decrease in Urbach's energy is the result of a decrease in the disorder of the biopolymer matrix and/or attributed to an increase in crystalline size. In addition, the cluster carbon number increased from 121.56 to 177.75, respectively, from bio-composite to bio-nanocomposite with 50% AgNPs. This is due to the presence of a strong H-binding interaction between the bio-composite matrix and the AgNPs molecules. The results revealed that the incorporation of 20% AgNPs into the CS:CL-CNC7:3-50% bio-composite film could be the best candidate composition for all optical properties. It can be used for potential applications in the area of food packaging as well as successfully on opto-electronic devices.

Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles

Preparation of various metal nanoparticles using plant extracts has been well studied in recent years. In this study, we found that nanoparticles synthesized using the extracts of the inflorescence of Cocos nucifera exhibited differential inhibitory activity against Escherichia coli depending on the nature of the bacterial culture source. Incorporation of silver nanoparticles (Ag-NPs) into the nutrient broth culture of E. coli resulted in poor inhibitory activity. However, when the silver nanoparticles are added to nutrient agar plates used for culture of E. coli, effective inhibition was observed. Additionally, E. coli in broth culture resisted the inhibitory effects of Ag-NPs by forming aggregates of bacterial cells. The aggregates then generated a protective zone around the colonies to prevent the entry of Ag-NPs, and the bacterial cells multiplied without inhibition by the Ag-NPs. These differential effects of Ag-NPs on E. coli culture grown in nutrient broth and on nutrient agar plates indicated that E. coli in broth culture formed aggregates of cells to develop a biofilm for protection against toxins probably via the quorum sensing mechanism.

The In-Vitro Effect of Silver and Zinc Oxide Nanoparticles on Fluoride Release and Microhardness of a Resin-Modified Glass Ionomer Cement

Reinforcement of nanoparticles into existing restorative biomaterials in dentistry is an area of interest. The aim of the current investigation was to incorporate silver nanoparticles (SNP) and zinc oxide nanoparticles (ZnONP) into a commercially available resin-modified glass ionomer cement (RMGIC). Their effects on the fluoride (F-) release from RMGIC were also investigated over a period of 14-days. Nanoparticles were incorporated at a loading concentration of 5 wt%, either individually or in a combination of both. Scanning electron microscopy (SEM), Fourier Transform infrared spectroscopy (FTIR), Nanocomputerized tomography (NanoCT), and the Vickers microhardness tester were used to examine the specimens. The fluoride release was analyzed by high performance liquid chromatography (HPLC). Data were analysed using ANOVA and Tukey’s test. RMGIC containing 5% ZnONP and 5% SNP + 5% ZnONP showed significant alterations in the surface ultrastructure with pores being evident in the surface. Fluoride release in parts per million (ppm) was highest in the 5% SNP and 5% ZnO-NP incorporated RMGIC compared to the control group and 5% SNP-incorporated RMGIC. RMGIC, as well as change in color observed in the 5% SNP incorporated RMGIC.

Multi-drug resistant ESKAPE pathogens and the uses of plants as their antimicrobial agents.

Infections by ESKAPE (Enterococcus sp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) pathogens cause major concern due to their multi-drug resistance(MDR). The ESKAPE pathogens are frequently linked to greater mortality, diseases, and economic burden in healthcare worldwide. Therefore, the use of plants as a natural source of antimicrobial agents provide a solution as they are easily available and safe to use. These natural drugs can also be enhanced by incorporating silver nanoparticles and combining them with existing antibiotics. By focussing the attention on the ESKAPE organisms, the MDR issue can be addressed much better.

Physicochemical, Morphological, and Cytotoxic Properties of Brazilian Jackfruit (Artocarpus heterophyllus) Starch Scaffold Loaded with Silver Nanoparticles.

Due to the physical, thermal, and biological properties of silver nanoparticles (AgNPs), as well as the biocompatibility and environmental safety of the naturally occurring polymeric component, polysaccharide-based composites containing AgNPs are a promising choice for the development of biomaterials. Starch is a low-cost, non-toxic, biocompatible, and tissue-healing natural polymer. The application of starch in various forms and its combination with metallic nanoparticles have contributed to the advancement of biomaterials. Few investigations into jackfruit starch with silver nanoparticle biocomposites exist. This research intends to explore the physicochemical, morphological, and cytotoxic properties of a Brazilian jackfruit starch-based scaffold loaded with AgNPs. The AgNPs were synthesized by chemical reduction and the scaffold was produced by gelatinization. X-ray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM-EDS), and Fourier-transform infrared spectroscopy (FTIR) were used to study the scaffold. The findings supported the development of stable, monodispersed, and triangular AgNPs. XRD and EDS analyses demonstrated the incorporation of silver nanoparticles. AgNPs could alter the scaffold's crystallinity, roughness, and thermal stability without affecting its chemistry or physics. Triangular anisotropic AgNPs exhibited no toxicity against L929 cells at concentrations ranging from 6.25 × 10-5 to 1 × 10-3 mol·L-1, implying that the scaffolds might have had no adverse effects on the cells. The scaffolds prepared with jackfruit starch showed greater crystallinity and thermal stability, and absence of toxicity after the incorporation of triangular AgNPs. These findings indicate that jackfruit is a promising starch source for developing biomaterials.

Evaluation of strategies to incorporate silver nanoparticles into electrospun microfibers for the preparation of wound dressings and their antimicrobial activity

ABSTRACT Currently, wound dressing technology is of significant interest in order to avoid undesired effects when burns or skin wounds are treated, especially their risk for microbial contamination and subsequent infection. Silver nanoparticles (AgNPs) have claimed importance as antimicrobial agents. For these reasons, a comparative study of different methods to synthesize electrospun polymeric fibers loaded with AgNPs (Ag+/PCLPVP) such as direct blending, ultraviolet irradiation, thermal treatment, and silver mirror reaction methods were done. The morphology and size of the fibers were investigated using the SEM, where the loading of the AgNPs in the fibers was measured with EDS analysis. FTIR, Raman spectroscopy, XRD, and TGA analysis were applied to measure the physicochemical properties of the fibers. DLS study allowed us to measure the size and surface charge of the obtained materials. The antimicrobial efficiency was carried out against Staphylococcus aureus and Escherichia coli bacteria for 24 h. Cytotoxicity was evaluated with the MTT assay using HFF1 cells. The results showed that the method with the best antimicrobial effect in both bacteria is the UV irradiation conducted before the electrospinning process. The UV radiation method also promotes good distribution efficiency, the physicochemical properties obtained were desirable for wound dressings, and the particle size within nanoparticle interval (~17 nm). Therefore, the most promising method for the preparation of Ag+/PCLPVPbased wound dressings was UV irradiation.

Ag nanoparticles incorporated interlayer enables ultrahigh critical current density for Li6PS5Cl-based all-solid-state lithium batteries

Agroydite Li6PS5Cl is one of the most promising solid electrolytes because of its high ionic conductivity and good processability. However, there are still many challenges with Li6PS5Cl and lithium anodes that prevent their industrialization, such as the growth of lithium dendrite and interfacial reactions. Interface modification of anodes has proven to be an effective method for solving these problems. Herein, we introduce Ag nanoparticles into the interface between Li6PS5Cl and metallic lithium and systematically investigate their interfacial properties. By incorporating silver nanoparticles, lithium dendrites can be depleted by forming Li–Ag alloys. Therefore, a denser and more stable interface between Li6PS5Cl and lithium metals is obtained. Benefiting from the above advantages, the Li/Li symmetrical battery with incorporated Ag nanoparticles exhibited an ultrahigh critical current density of up to 10 mA cm−2 and great cycling stability (more than 600 h). An all-solid-state battery with Ni-rich cathode and lithium metal anode demonstrates a high-capacity retention rate of 84% after 200 cycles at a current density of 0.5C. This study provides new insights into the construction of stable anode interfaces for the practical application of all-solid-state lithium batteries.

Nile Tilapia Skin Impregnated with Antibacterial Silver/Titanium Dioxide Compounds

The development of alternative (and free-of-antibiotics) antibacterial and antibiofilm agents is an important strategy to circumvent the resistance of bacteria to antibiotics. Herein, we explore the production of mixed oxides by incorporating silver nanoparticles in titanium dioxide as a silver concentration-dependent antibacterial agent that is further incorporated in Tilapia fish skin (a promising prototype of xenograft), integrating the antibacterial activity of mixed oxide into the intrinsic properties of Tilapia skin. The antibiofilm activity of samples prepared with high concentrations of silver (10 wt% of precursor AgNO3) has been considered a good antibiofilm response. The influence of silver content is also observed with respect to the minimum bactericidal concentration, which is reduced to 3.13 mg/mL with a characteristic kill time in the order of 30 min that is associated with antibiofilm activity in biofilm-forming strains of Staphylococcus aureus. These results indicate that modified Tilapia fish skin acquires antibacterial behavior and can be explored for xenografts with prospective applications in the light-dependent actuation of TiO2-based compounds.

Incorporation of silver nanoparticles into polyamide powder for application in 3D printing

Incorporation of silver nanoparticles into polyamide powder for application in 3D printing

Investigation of the hydration process and biological activity of a novel nanosilver incorporated dicalcium silicate based retrograde filling material.

Although several materials have been used for retrograde filling following apical surgeries, there is no consensus on a single best material. Tricalcium silicate-based types of cement have been developed as root-end filling materials mainly due to tricalcium silicate's hydraulic properties. However, its unfavorable setting characteristics and minimal antimicrobial properties have necessitated the introduction of new additives into the existing commercially available materials. To design an affordable product based on a dicalcium silicate with a shorter set time, minimal cytotoxic complications, and enhanced antibacterial activity, we developed a new endodontic cement from pure raw materials, intending to satisfy the prerequisites of ideal retrograde material. The composition of the experimental calcium silicate-based cement included the addition of calcium chloride and silver nanoparticles in varying concentrations. Structural characterization was carried out using energy dispersive analysis by X-rays using scanning electron microscope (EDAX SEM) and hydration characteristics were performed using an X-ray diffractometer (XRD). The experimental material was further evaluated for biocompatibility using MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide)assay and antibacterial activity was evaluated using an agar diffusion test against Enterococcus faecalis. The structural characterization and hydration characteristics revealed that the experimental cement was dicalcium silicate based with favorable biocompatibility and enhanced antibacterial activity. Tricalcium silicate based mineral trioxide aggregate (MTA) also had favourable biocompatibility, however, its antibacterial activity was significantly decreased when compared to the novel cement. All hydraulic cements that are available in the dental market are predominantly tricalcium silicate-based materials. There has been no evidence in the literature to date wherein it has been explored whether a dicalcium silicate-based hydraulic cement can solely be used in root-end cavities. The findings of the study revealed a dicalcium silicate based retrograde filling material with favourable biocompatibility exhibited immediately as well as in the set samples. Incorporation of silver nanoparticles boosted the antibacterial activity when compared to that of ProRoot MTA. This material could potentially reinstate the usual hype created with tricalcium silicate types of cement since dicalcium silicate cements also exhibit similar properties.

Antimicrobial Polymeric Surfaces Using Embedded Silver Nanoparticles.

Pathogens (disease-causing microorganisms) can survive up to a few days on surfaces and can propagate through surfaces in high percentages, and thus, these surfaces turn into a primary source of pathogen transmission. To prevent and mitigate pathogen transmission, antimicrobial surfaces seem to be a promising option that can be prepared by using resilient, mass-produced polymers with partly embedded antimicrobial nanoparticles (NPs) with controlled size. In the present study, a 6 nm thick Ag nanolayer was sputter deposited on polycarbonate (PC) substrate and then thermally annealed, in a first step at 120 °C (temperature below Tg) for two hours, for promoting NP diffusion and growth, and in a second step at 180 °C (temperature above Tg) for 22 h, for promoting thermal embedding of the NPs into the polymer surface. The variation in the height of NPs on the polymer surface with thermal annealing confirms the embedding of NPs. It was shown that the incorporation of silver nanoparticles (Ag NPs) had a great impact on the antibacterial capacity, as the Ag NP-embedded polymer surface presented an inhibition effect on the growth of Gram-positive and Gram-negative bacteria. The tested surface-engineering process of incorporating antimicrobial Ag NPs in a polymer surface is both cost-effective and highly scalable.

Effect of silver nanoparticles incorporation on impact strength of Heat-cure denture base resins

Background:Poly (Methyl methacrylic acid) based materials are widely used for the fabrication of removable complete and partial denture prostheses. these materials are prone to fractures when subjected to impact forces. While recent studies have explored the antimicrobial potential of denture base materials incorporating silver nanoparticles, their impact on the mechanical properties crucial for the prosthesis's durability has not been adequately established.Aim:To evaluate the impact strength of the heat-cure denture base materials incorporated with various concentrations of silver nanoparticles. Materials and Methods:Silver nanoparticles were incorporated at various concentrations (0.5, 1.0, 2.0 and 5.0 wt%) into three different heat-cure denture base materials. A total of 150 rectangular-shaped specimens (50 x 6 x 4), which comprises 50 samples from each of the three heat-cure acrylic resins were made using the compression molding technique. Ten specimens (n=10) were allocated for each concentration such as control, 0.5wt%, 1.0wt%, 2.0wt% and 5.0wt% concentrations of silver nanoparticles. A “V” notch was made exactly at the centre of each specimen to prevent the ductile fracture. The impact strength was evaluated using the Izod impact tester. The data were subjected to One-way ANOVA and Post hoctests for statistical analyses.Results: A decrease in the impact strength was observed in the modified groups compared to the control groups. One-way ANOVA showed significant differences (p=0.000) among the different concentrations of all three denture base materials.Conclusion:Silver nanoparticles incorporation reduced the impact strength of the denture base materials.

Synergistic Effect of Incorporation of BG 45S5 and Silver Nanoparticles on β-TCP Scaffolds: Structural Characterization and Evaluation of Antimicrobial Activity and Biocompatibility

Synergistic Effect of Incorporation of BG 45S5 and Silver Nanoparticles on β-TCP Scaffolds: Structural Characterization and Evaluation of Antimicrobial Activity and Biocompatibility

Effect of Silver Nanoparticles of Herbal Origin on the Compressive and Push-out Bond Strengths of Mineral Trioxide Aggregate.

The purpose of this in vitro study was to investigate the effect of incorporating silver nanoparticles (AgNPs) of herbal origin into mineral trioxide aggregate (MTA) on the push-out bond strength (PBS) and compressive strength (CS) in simulated furcal area perforations. In this in vitro study, simulated furcal area perforations (1.3 mm in diameter and 2 mm in depth) were created in 40 extracted human lower molar teeth, which were divided into two groups (n=20): MTA alone and MTA combined with AgNPs (2% wt). Using a universal testing machine, PBS was evaluated by performing push-out tests, while CS was assessed using cylindrical specimens. The normal distribution of data was checked using the Kolmogorov-Smirnov test, and statistical analysis was performed using two-way ANOVA. The CS results showed no significant difference between the MTA group at 4 and 21 days (P=0.297), but a significant difference was observed in the nanosilver/MTA group (P=0.013). However, there was no significant difference in the push-out bond strength among the study groups (P>0.05). The incorporation of herbal origin silver nanoparticles did not significantly affect the PBS or CS of MTA.