A new method for estimating the accuracy of a representative ecological sample (species minimal area and abundance minimal area determined by similarity area curves) is presented. Formulas are derived to calculate the adequate similarity level (IS, respectively IK) in relation to the desired accuracy of the sample: IS=2 w/1+w for qualitative similarity and ≧K=2 x/(1+x)(1+y) for quantitative similarity, where w=desired qualitative accuracy of the sample, x=desired quantitative accuracy of the sample, y=abundance proportion of rare species. Based on samples from a low diversity environment in the supralittoral fringe of rocky shores (“black zone”), the new approach was tested and led to the following results: (1) Similarity area curves are an objective method to calculate the minimal area and to test the accuracy of the sample size chosen. (2) A large presample is necessary to calculate the minimal area. (3) The size of minimal area is subject to seasonal and regional variations. (4) Incidental species lead to an overestimation of the species minimal area. (5) Highly dominant species lead to an underestimation of the abundance minimal area.
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