Improved Blood Compatibility Research Articles

Electrospun polyethylene terephthalate (PET) nanofibrous conduit for biomedical application

Nanostructured biomaterials have great potential in the field of biomedical engineering. Efforts for treatment of cardiovascular diseases focused on introducing vascular substitutes that are nonthrombogenic and have long‐term patency, but still there is not any perfect replacement for clinical use. In this study, nanostructure tubes of a commonly known biocompatible polymer, polyethylene terephthalate (PET), were prepared via electrospinning process using small diameter mandrel as a collector with two different speeds. The nanofibers (NFs) morphologies' physical and mechanical properties were investigated according to scanning electron microscope (SEM), water contact angle (WCA), porosity measurement, differential scanning calorimetry (DSC), and tensile test. Finer NFs, more percentage of crystallinity, and superior mechanical properties were observed for samples prepared by higher speed mandrel. Since both samples stimulated platelet adhesion and activation, further surface modification with sodium nitrate as nitric oxide (NO) donor was done using two different approaches: dip‐coating and electrospraying. The modified NFs were evaluated via SEM, WCA, tensile test, platelets, and cell adhesion. The results showed more hydrophilicity, reduction in platelet adhesion, and improved blood compatibility for eNO‐HS (electrosprayed NO for higher collector speed) compared with other samples implying the promising potential of this fabrication and modification technique for improving PET‐based cardiovascular substitutes.

Ultralow fouling of fibrinogen and human platelets on ulvan multilayer-coated solid surfaces

Enhancement of blood compatibility with the implant surface is critical for biomedical devices. Coating solid surfaces with an anticoagulant is a potentially promising strategy to improve blood compatibility with the implant surface. This study aimed to develop an approach to enhance blood compatibility with titanium/titanium dioxide (Ti/TiO2) surfaces using a green seaweed-derived polysaccharide ulvan. The approach comprises surface coating with tannic acid (TA) and grafting of ulvan onto the surface. Zirconium(IV)-based coordination linkage between ulvan and TA facilitated robust deposition of ulvan multilayers onto Ti/TiO2 surfaces. Adhesion assays revealed that more than 95% of fibrinogen adsorption and platelet adhesion was suppressed through an ulvan multilayer coating. The present results show that ulvan multilayers are robust against chemical and physical treatments and resistant to the adsorption of adherent proteins and human platelets.

Persulfated flavonoids accelerated re-endothelialization and improved blood compatibility for vascular medical implants

Drug-eluting stents (DESs) have been used for the treatment of cardiovascular diseases including stenosis. However, in-stent restenosis, thrombosis, and delayed re-endothelialization represent challenges for their clinical applications. Here, we demonstrate a novel work to overcome these limitations through surface modification technology. The cobalt-chromium (Co-Cr) surface was modified with antioxidants such as gallic acid (GA) and rutin (Ru) and the corresponding persulfates derivatives (i.e., GAS, and RuS) through a simple conjugation procedure. Various analyses tools such as ATR-FTIR, XPS, water contact angle, SEM, and AFM characterized the functionalized surface. The surface characterization confirmed that the antioxidant and the additional persulfates were successfully bonded to the Co-Cr surface. The results of in vitro endothelial cells proved that the persulfates derivatives showed the highest tendency to get rapid re-endothelialization especially RuS. In addition, it showed inhibition to smooth muscle cells (SMCs) as compared to control Co-Cr substrate. The persulfates modified substrates reduced the amount of adsorbed fibrinogen and albumin with higher stability to fetal bovine serum. Moreover, platelet study also demonstrated that Ru and RuS presented lower platelet adhesion with round shape morphology, whereas the control Co-Cr adhere and activate many platelets with pseudopodium morphology. Moreover, these modification processes did not cause any inflammatory responses. In conclusion, it is believed that the persulfates flavonoids have a great potential in the field of drug-eluting stents and blood contacting medical implants to improve blood compatibility, suppress SMCs, and get rapid re-endothelialization.

An Albumin Biopassive Polyallylamine Film with Improved Blood Compatibility for Metal Devices.

Nowadays, a variety of materials are employed to make numerous medical devices, including metals, polymers, ceramics, and others. Blood-contact devices are one of the major classes of these medical devices, and they have been widely applied in clinical settings. Blood-contact devices usually need to have good mechanical properties to maintain clinical performance. Metal materials are one desirable candidate to fabricate blood-contact devices due to their excellent mechanical properties and machinability, although the blood compatibility of existing blood-contact devices is better than other medical devices, such as artificial joints and artificial crystals. However, blood coagulation still occurs when these devices are used in clinical settings. Therefore, it is necessary to develop a new generation of blood-contact devices with fewer complications, and the key factor is to develop novel biomaterials with good blood compatibility. In this work, one albumin biopassive polyallylamine film was successfully established onto the 316L stainless steel (SS) surface. The polyallylamine film was prepared by plasma polymerization in the vacuum chamber, and then polyallylamine film was annealed at 150 °C for 1 h. The chemical compositions of the plasma polymerized polyallylamine film (PPAa) and the annealed polyallylamine film (HT-PPAa) were characterized by Fourier transform infrared spectrum (FTIR). Then, the wettability, surface topography, and thickness of the PPAa and HT-PPAa were also evaluated. HT-PPAa showed increased stability when compared with PPAa film. The major amino groups remained on the surface of HT-PPAa after annealing, indicating that this could be a good platform for numerous molecules’ immobilization. Subsequently, the bovine serum albumin (BSA) was immobilized onto the HT-PPAa surface. The successful introduction of the BSA was confirmed by the FTIR and XPS detections. The blood compatibility of these modified films was evaluated by platelets adhesion and activation assays. The number of the platelets that adhered on BSA-modified HT-PPAa film was significantly decreased, and the activation degree of the adhered platelets was also decreased. These data revealed that the blood compatibility of the polyallylamine film was improved after BSA immobilized. This work provides a facile and effective approach to develop novel surface treatment for new-generation blood-contact devices with improved hemocompatibility.

Fabrication and characterization of polyurethane patch loaded with palmarosa and cobalt nitrate for cardiac tissue engineering

Cardiac patches are attractive option in overcoming the morbidities associated with cardiac disorders. Nanofibrous scaffolds were fabricated using polyurethane (PU) added with palmarosa (PR) and cobalt nitrate (CoNO3) using an electrospinning technique. Several characterizations were employed namely field emission scanning electron microscopy, wettability measurement, attenuated total reflectance infrared spectroscopy, thermal analysis, surface roughness measurements, and tensile testing. Further, biological response of the electrospun nanofibers were tested through coagulation study and MTS assay. As-spun composite mats showed smaller fibers than pure PU as depicted in morphology analysis. The interaction of PU with PR and CoNO3 was confirmed in infrared spectrum and thermal analysis. The incorporation of the PR decreased the wettability and while CoNO3 addition resulted in the hydrophilic nature as depicted in the contact angle measurements. Mechanical properties testing showed that elongation at break for the pristine PU was increased with the addition of PR and CoNO3. The surface measurements depicted that the incorporation of PR resulted in the improvement of the surface roughness while the addition of CoNO3 reduced the surface roughness of the pristine PU. The electrospun nanocomposites showed delayed blood clotting time compared to the pristine PU as shown in coagulation study. Both composites supported the better proliferation of fibroblast cells than pure PU. Therefore, novel composites with smaller fiber diameter, hydrophilicity, better mechanical properties, improved blood compatibility parameters, and good cell viability rates would be a promising candidate for cardiac tissue engineering.

Improved blood compatibility and cyto-compatibility of Zn-1Mg via plasma electrolytic oxidation

Compared to magnesium (Mg) or iron (Fe) based biodegradable alloys, Zinc (Zn) alloys exhibit moderate degradation rates, thus regarded as promising implant materials for orthopedic and cardiovascular applications. However, Zn2+ released from degradation process may result certain degrees of cytotoxicity. Thus it is necessary to modify the surface of biodegradable Zn alloys for better biocompatibility. In this paper, a porous ceramic coating was successfully prepared on a Zn-1Mg alloy using plasma electrolytic oxidation (PEO). The effect of the PEO coating on the corrosion resistance and in vitro cyto-compatibility of the Zn-1Mg alloy were evaluated. It was noted that the thickness of the PEO coatings could be adjusted from 6.7 ± 0.5 µm to 28.6 ± 2 µm by increasing the oxidation time from 30 s to 120 s. Longer oxidation time led to a more rough and hydrophobic surface, which was consisted of ZnO and Zn2SiO4. The stable and protective oxide layer enhanced the corrosion resistance of the Zn-1Mg alloy. The PEO coated Zn-1Mg exhibited better blood compatibility and cyto-compatibility. Decreased hemolytic ratio and lower platelet adhesion was observed on the PEO coated Zn-1Mg sample. The enhanced cyto-biocompatibility is mainly attributed to small amount of Si2+ and Mg2+ released during the early degradation of the PEO coating.

Superhydrophobic blood-compatible surfaces: state of the art

Various strategies have been developed to improve blood compatibility of biomaterials, mostly through surface modification techniques. Superhydrophobic surfaces are among the promising approaches controlling the interaction of proteins and cells with external surfaces in medical applications. This review summarizes the recent studies on hemocompatibility of superhydrophobic surfaces. The review begins with principle theories on wettability of surfaces, and then evaluates the interaction of blood proteins and platelets with superhydrophobic surfaces and discusses the conflicting results. Finally, future challenges in utilizing superhydrophobic surfaces for blood contacting applications are also addressed.

Cyclodextrin Reduces Intravenous Toxicity of a Model Compound

Solubilization of new chemical entities for toxicity assessment must use excipients that do not negatively impact drug pharmacokinetics and toxicology. In this study, we investigated the tolerability of a model freebase compound, GDC-0152, solubilized by pH adjustment with succinic acid and complexation with hydroxypropyl-β-cyclodextrin (HP-β-CD) to enable intravenous use. Solubility, critical micelle concentration, and association constant with HP-β-CD were determined. Blood compatibility and potential for hemolysis were assessed in vitro. Local tolerability was assessed after intravenous and subcutaneous injections in rats. A pharmacokinetic study was conducted in rats after intravenous bolus administration.GDC-0152 exhibited pH-dependent solubility that was influenced by self-association. The presence of succinic acid increased solubility in a concentration-dependent manner. HP-β-CD alone also increased solubility, but the extent of solubility enhancement was significantly lower than succinic acid alone. Inclusion of HP-β-CD in the solution of GDC-0152 improved blood compatibility, reduced hemolytic potential by ∼20-fold in vitro, and increased the maximum tolerated dose to 80 mg/kg.

Chitosan-heparin nanoparticle coating on anodized NiTi for improvement of blood compatibility and biocompatibility.

The aim of this study was to simultaneously improve blood compatibility and corrosion resistance of nitinol via two-step process; anodizing and consequently coating with chitosans-heparin nanoparticles. Moreover, the role of these surface modification processes on the heparin release kinetic and blood compatibility was investigated. Finally, the interaction between human umbilical vein endothelial cells (HUVECs) and surface modified samples was investigated. Electrochemical characterization revealed that while Ni ions released from the anodized sample (9 ppb), chitosan-heparin nanoparticle coatings prohibited from Ni ion release form NiTi substrate. Moreover, the controlled release of heparin was found from chitosan-heparin nanoparticle coating deposited on the nanotubes, leading to significant improvement of blood compatibility. Finally, HUVECs were attached and proliferated on the chitosan-heparin nanoparticle coated samples confirming the cell compatibility of samples. In summary, results proved that two-step anodizing process and heparin release could promote both endothelial cell compatibility and blood compatibility to nitinol surface which might be appropriate for coronary stent application.

Electrospun Bilayer Composite Vascular Graft with an Inner Layer Modified by Polyethylene Glycol and Haparin to Regenerate the Blood Vessel.

In this study, we prepared a composite vascular graft with two layers. The inner layer, which was comprised of degradable Poly(lactic-co-glycolic acid) (PLGA)/Collagen (PC) nanofibers modified by mesoporous silica nanoparticles (MSN), was grafted with polyethylene glycol (PEG) and heparin to promote cell proliferation and to improve blood compatibility. The outer layer was comprised of polyurethane (PU) nanofibers in order to provide mechanical support. The growth and proliferation of human umbilical vein endothelial cells (HUVECs) in the inner layer was significant, and blood compatibility testing showed that the inner layer had good blood compatibility. The MSN-PEG-Heparin on the fiber surface was observed in vitro during the degradation of the inner layer. After 60 days, the weight of fiber membrane decreased by 92.4%. The inner layer did not cause an inflammatory reaction during the degradation process in vivo and there was uniform cellular growth on the PC/MSN-PEG-Heparin fiber membrane. Composite grafts implanted into the rabbit carotid artery were evaluated for 8 weeks by H&E and immunohistochemical staining, demonstrating that a monolayer of endothelium (CD31-labeled) and smooth muscle (αSMA-labeled) regenerated on the composite graft. Our results demonstrate that the composite graft, with a functional inner layer, has potential to be used for small-caliber blood vessels with long-term patency.

Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization.

Two anticoagulant proteins, nattokinase and lumbrokinase, were successfully immobilized onto the dopamine-coated polysulfone (PSf) membrane surface by covalent bonding. X-ray photoelectron spectroscopy (XPS) and Zeta potential measurement confirmed the immobilization of these anticoagulant proteins. The immobilization yield of nattokinase and lumbrokinase reached to 35.2 and 33.4 μg/cm2, respectively. The water contact angle measurement revealed that the membrane surface hydrophilicity was improved after immobilizing the anticoagulant proteins. Meanwhile, the immobilized proteins retained their biological activity. Then blood compatible tests demonstrated that the modified membranes had lower static protein adsorption, platelet/erythrocyte adhesion, hemolysis, as well as longer blood clotting time, compared to virgin PSf membrane. In addition, the nattokinase-immobilized membrane showed a higher blood compatibility than BSA and lumbrokinase immobilized memrbanes, due to its' high bioactivity. Our research demonstrates that the dopamine-assisted immobilization of nattokinase and lumbrokinase can endow the membranes with improved blood compatibility as well as high biological activity, which may be expected to apply to blood-contacting materials.

Enriched mechanical, thermal, and blood compatibility of single stage electrospun polyurethane nickel oxide nanocomposite for cardiac tissue engineering

Electrospinning is a versatile technique for producing nanocomposite in biomedical applications. In this study, polyurethane (PU) cardiac patch loaded with nickel oxide (NiO) was fabricated using electrospinning technique. The morphology study revealed that the PU/NiO nanocomposites exhibited reduced fiber diameter (758 ± 157.10 nm) and pore diameter (868 ± 73.26 nm) compared to the pristine PU (fiber diameter 890 ± 116.91 nm and pore diameter 1064 ± 74.31 nm). The contact angle study indicated the decreased wettability behavior of the electrospun nanocomposite (106° ± 0.58°) than the pure PU (100° ± 0.58°). The incorporation of NiO into PU improved the mechanical strength (15.25 MPa) and surface roughness (448 nm) of the pristine PU (tensile strength 7.12 MPa and surface roughness 313 nm). The developed PU/NiO nanocomposites showed delayed blood clotting time and low hemolytic percentage as revealed in the coagulation studies insinuating the improved anticoagulant nature compared to the pristine PU. Moreover, 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium, inner salt (MTS) assay demonstrated the nontoxic behavior of fibroblast cells in the developed nanocomposite (135 ± 1%) than the pristine PU (133.33 ± 8.96%). The newly developed nanocomposite patch rendered better physicochemical, improved blood compatibility, and nontoxic to the fibroblast cells. Hence, the electrospun PU/NiO nanocomposite might serve as a plausible scaffold for the cardiac tissue engineering. POLYM. COMPOS., 40:2381–2390, 2019. © 2018 Society of Plastics Engineers

Etoposide encased folic acid adorned mesoporous silica nanoparticles as potent nanovehicles for enhanced prostate cancer therapy: synthesis, characterization, cellular uptake and biodistribution

The present research was motivated by the dire need to design a targeted and safe Nano-vehicle for delivery of Etoposide (ETE), which would be tolerant of normal cells and exclusively toxic to prostate cancer cells. The folic acid functionalized mesoporous silica nanoparticles (MSNs) constructed by using a facile method acting as a unique selective platform for ETE delivery for effective prostate cancer treatment. FA@MSNs possessed good payload and encouraging in vitro release was obtained for ETE caged inside FA-MSNs compared with ETE-MSNs alone. Further, FA@MSNs exhibited an improved blood compatibility compared with pristine silica. The cellular analysis on PC-3 and LNCaP cell lines unveiled an excellent performance of cytotoxicity. Apoptosis assay confirmed a programmed cell death ruling out necrosis. Most importantly enhanced cellular uptake was obtained for FITC#FA@MSNs. In addition, pharmacokinetic and biodistribution studies in healthy mice indicated a favourable longer circulation time and reduced plasma elimination rate for ETE/FA@MSNs than free ETE. Further, histological and cell cytotoxicity results proved that nanocarriers themselves were safe without any noticeable toxicity. The results showed that FA@MSNs were ideal candidates for safe and effective delivery of ETE and hold a substantial potential as drug delivery vehicles for enhanced prostate cancer therapy.

Heparinized PCL/keratin mats for vascular tissue engineering scaffold with potential of catalytic nitric oxide generation

Heparins are capable of improving blood compatibility, enhancing HUVEC viability, while inhibiting HUASMC proliferation. Combination of biodegradable poly(ε-caprolactone) (PCL) with keratin and heparins would provide an anticoagulant and endothelialization supporting environment for vascular tissue engineering. Herein, PCL and keratin were first coelectrospun and then covalently conjugated with heparins. The resulting mats were surface-characterized by ATR-FTIR, SEM, WCA, and XPS. Cell viability data showed that the heparinized PCL/keratin mats could motivate the adhesion and growth of HUVEC, while inhibit HUASMC proliferation. In addition, these mats could prolong blood clotting time and reduce platelet adhesion as well as no erythrolysis. Interestingly, these mats could catalyze the NO donor in blood to release NO, which could enhance endothelial cell growth, while decrease smooth muscle cell proliferation and platelet adhesion. In summary, the heparinized mats would be a good candidate as a scaffold for vascular tissue engineering. This study is novel in that we prepared a type of heparinized tissue scaffold that could catalyze the NO donor to release NO to regulate endothelialization without angiogenesis and thrombus formation.

Molecular dynamics study of the molecular mobilities and side-chain terminal affinities of 2-methoxyethyl acrylate and 2-hydroxyethyl methacrylate

Irrespective of the degree of polymerization, the molecular mobilities of the 2-hydroxyethyl methacrylate (HEMA) moieties are smaller than those of the 2-methoxyethyl acrylate (MEA) moieties. Preventing the polar functional groups of the foulants and materials from forming a hydrogen-bonding network is important to enhance the mobilities of the molecular chains of non-ionic polymeric materials. We speculate that enhancing the mobilities of the molecular chains is key to improving blood compatibility.

Heparin as a molecular spacer immobilized on microspheres to improve blood compatibility in hemoperfusion

Heparin, a highly sulfated linear polysaccharide, with anticoagulation function and blood compatibility is widely used as a biomaterials in medical application, but the most importance of heparin is its structure function as the macromolecular space arm. In this study, heparin as a spacer was covalently immobilized on the chloromethylated polystyrene microspheres (Ps) and then connected with l-phenylalanine forming the Ps-Hep-Phe structure, which was developed for endotoxin adsorption in hemoperfusion. The grafting density of heparin reach the maximum when the initial concentration of heparin solution was 5 mg/mL. The adsorbents with the heparin as a spacer showed the prolonged clotting times, low protein adsorption, and reduced the hemolysis rate, indicating that heparin-modified adsorbents have great blood compatibility. The adsorption capacity of Ps-Hep-Phe for endotoxin was 25.15 EU/g in dynamic adsorption, higher than that of Ps. Therefore, this study imply that heparin would be promising for modification of adsorbents in hemoperfusion.

Bio-Corrosion Behavior of Ceramic Coatings Containing Hydroxyapatite on Mg-Zn-Ca Magnesium Alloy

Ceramic coatings containing hydroxyapatite (HA) were fabricated on a biodegradable Mg66Zn29Ca5 magnesium alloy through micro-arc oxidation by adding HA particles into the electrolytes. The phase composition and surface morphology of the coatings were characterized by X-ray diffraction and scanning electron microscopy analyses, respectively. Electrochemical experiments and immersion tests were performed in Hank’s solution at 37 °C to measure the corrosion resistance of the coatings. Blood compatibility was evaluated by in vitro blood platelet adhesion tests and static water contact angle measurement. The results show that the typical ceramic coatings with a porous structure were prepared on the magnesium alloy surface with the main phases of MgO and MgSiO3 and a small amount of Mg3(PO4)2 and HA. The optimal surface morphology appeared at HA concentration of 0.4 g/L. The electrochemical experiments and immersion tests reveal a significant improvement in the corrosion resistance of the ceramic coatings containing HA compared with the coatings without HA or bare Mg66Zn29Ca5 magnesium alloy. The static water contact angle of the HA-containing ceramic coatings is 18.7°, which is lower than that of the coatings without HA (40.1°). The in vitro blood platelet adhesion tests indicate that the HA-containing ceramic coatings possess improved blood compatibility compared with the coatings without HA. Utilizing HA-containing ceramic coatings may be an effective way to improve the surface biocompatibility and corrosion resistance of magnesium alloys.

In vitro biocompatibility analysis of functionalized poly(vinyl chloride)/layered double hydroxide nanocomposites.

The aim of this study was to examine the cytotoxicity and biocompatibility of functionalized poly(vinyl chloride) (PVC)/layered double hydroxide (LDH) nanocomposites. The biocompatibility of the LDH-based nanocomposites of thiosulphate PVC (TS-PVC), thiourea PVC (TU-PVC) and sulphite PVC (S-PVC) was assessed via haemolysis and thrombogenicity tests followed by the analysis of cellular adhesion and proliferation. The MTT assay was performed on cells in direct contact with the polymeric nanocomposites to evaluate the side effects of the biomaterials. The cellular morphology of mouse mesenchymal stem cells was also analyzed after incubation with direct contact with the functionalized polymer nanocomposites for different time periods. Although the results of the haemolysis test displayed a positive influence of LDH on the functionalized PVC compared to the neat PVC, the thrombogenic property was observed to be notably decreased, which indicated improved blood compatibility. The resulting LDH samples were also studied for their performance via fluorescence imaging of cells after incubation with the materials. The LDH-based polymers exhibited an excellent level of cytocompatibility, which validates their use as biomaterials. PVC-TU/LDH-2 and PVC-S-2 were found to be notably less cytotoxic for the tested cell type. Also, the cells were found to adhere better to the entire PVC-S/LDH nanocomposite surface. The cytotoxicity test also revealed that the PVC-TU/LDH and PVC-S/LDH nanocomposites exhibited similar responses. The fluorescence-based image analysis showed that cells were spread much more on the polymer surface containing a higher LDH weight percentage. Overall, this study provides a benchmark for the biocompatibility properties of PVC/LDH nanocomposites, which may be useful for numerous applications in the biomedical and related areas.

Improve blood compatibility of bioresorbable magnesium stents coated with functionalized anti-CD34 antibody and heparin-collagen multiplayers.

Instentrestenosis and late stent thrombosis continue to be the greatest limitations of drug-eluting stents. In this study, an anti-CD34 antibody functionalized multilayer of heparin/collagen type I is developed by layer-by-layer assemble to enhance biocompatibility and recruit endothelial progenitor cells. The SEM results demonstrate that (Hep/Col)5-coated samples have a rougher surface compared to (Hep/Col)2-coated samples and untreated samples. The EDX and FTIR results both confirmed the presence of heparin and collagen onto the modified samples. The immunofluorescence staining outcomes demonstrated that anti-CD34 antibody has successfully immobilized onto modified Mg discs.

Mesoporous Silica Nanoparticles-Encapsulated Agarose and Heparin as Anticoagulant and Resisting Bacterial Adhesion Coating for Biomedical Silicone.

Silicone catheter has been widely used in peritoneal dialysis. The research missions of improving blood compatibility and the ability of resisting bacterial adhesion of silicone catheter have been implemented for the biomedical requirements. However, most of modification methods of surface modification were only able to develop the blood-contacting biomaterials with good hemocompatibility. It is difficult for the biomaterials to resist bacterial adhesion. Here, agarose was selected to resist bacterial adhesion, and heparin was chosen to improve hemocompatibility of materials. Both of them were loaded into mesoporous silica nanoparticles (MSNs), which were successfully modified on the silicone film surface via electrostatic interaction. Structures of the mesoporous coatings were characterized in detail by dynamic light scattering, transmission electron microscopy, Brunauer-Emmett-Teller surface area, thermogravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscope, and water contact angle. Platelet adhesion and aggregation, whole blood contact test, hemolysis and related morphology test of red blood cells, in vitro clotting time tests, and bacterial adhesion assay were performed to evaluate the anticoagulant effect and the ability of resisting bacterial adhesion of the modified silicone films. Results indicated that silicone films modified by MSNs had a good anticoagulant effect and could resist bacterial adhesion. The modified silicone films have potential as blood-contacting biomaterials that were attributed to their biomedical properties.