Impact Of Microplastics Research Articles

Polyethylene microplastics alter soil microbial community assembly and ecosystem multifunctionality

Although pervasive microplastics (MPs) pollution in terrestrial ecosystems invites increasing global concern, impact of MPs on soil microbial community assembly and ecosystem multifunctionality received relatively little attention. Here, we manipulated a mesocosm experiment to investigate how polyethylene MPs (PE MPs; 0, 1%, and 5%, w/w) influence ecosystem functions including plant production, soil quality, microbial community diversity and assembly, enzyme activities in carbon (C), nitrogen (N) and phosphorus (P) cycling, and multifunctionality in the maize–soil continuum. Results showed that PE MPs exerted negligible effect on plant biomass (dry weight). The treatment of 5% PE MPs caused declines in the availability of soil water, C and P, whereas enhanced soil pH and C storage. The activity of C-cycling enzymes (α/β-1, 4-glucosidase and β-D-cellobiohydrolase) was promoted by 1% PE MPs, while that of β-1, 4-glucosidase was inhibited by 5% PE MPs. The 5% PE MPs reduced the activity of N-cycling enzymes (protease and urease), whereas increased that of the P-cycling enzyme (alkaline phosphatase). The 5% PE MPs shifted soil microbial community composition, and increased the number of specialist species, microbial community stability and networks resistance. Moreover, PE MPs altered microbial community assembly, with 5% treatment decreasing dispersal limitation proportion (from 13.66% to 9.96%). Overall, ecosystem multifunctionality was improved by 1% concentration, while reduced by 5% concentration of PE MPs. The activity of α/β-1, 4-glucosidase, urease and protease, and ammonium-N content were the most important predictors of ecosystem multifunctionality. These results underscore that PE MPs can alter soil microbial community assembly and ecosystem multifunctionality, and thus development and implementation of practicable solutions to control soil MPs pollution become increasingly imperative in sustainable agricultural production.

Soil Biochemical Indicators to Monitor the Impact of Microplastics on Soil Functionality in Terrestrial Ecosystems.

The present paper introduces soil as a complex system, so a multidisciplinary approach is needed to study not only the composition, abundance, and transport of microplastics (MPs) in terrestrial ecosystems but also soil properties and processes involved in their degradation and/or interaction with soil polyphasic matrix. Despite many researchers focusing their studies on the impact of MPs on the terrestrial ecosystem over the past years, little has been done about the use of biochemical indicators to study their effect on soil functionality.

Effects of microplastics on the environmental behaviors of the herbicide atrazine in soil: Dissipation, adsorption, and bioconcentration

As an emerging contaminant in soil, the impact of microplastics (MPs) on the environmental behavior of other organic pollutants remains uncertain, potentially threatening the sustainability of agricultural production. In this study, the impact of two kinds of MPs on the environmental behaviors of herbicide atrazine in soil-plant system was investigated. The results showed that MPs significantly reduced the half-life 17.69 ∼ 21.86 days of atrazine in the soil, compared to the control group. Meanwhile, the introduction of MPs substantially increased atrazine adsorption. Additionally, MPs substantially enriched the diversity and functionality of soil microbiome, and the soil metabolic activity was stimulated. Regarding the crop growth, the accumulation of atrazine in maize were significantly decreased by approximately 48.4–78.5 % after exposure to MPs. In conclusion, this study reveals the impact of MPs on atrazine's environmental behaviors in soil and highlights their less effect on maize growth, providing valuable insights for managing MPs contamination in sustainable agriculture.

Distinct dynamics in mountain watersheds: Exploring mercury and microplastic pollution—Unraveling the influence of atmospheric deposition, human activities, and hydrology

The intensification of human activities all around the globe has led to the spread of micropollutants in high-mountain freshwater environments. We therefore aimed to assess the geospatial distribution and determine the potential sources of (total-) mercury (THg) and microplastics (MPs) in mountain freshwater ecosystems. To do so, we analyzed THg and MP concentrations in brown trout, biofilm, and sediments from lotic and lentic ecosystems in the Pyrenees – all subjected to different types of human pressure. Additionally, we assessed the potential impacts of these pollutants on fish, and explored the bioindication capacity of brown trout (Salmo trutta fario) and biofilm regarding THg and MP pollution. For the first time, we measured concentrations of MPs trapped in the matrix of freshwater biofilm. Our results suggest that THg in the Pyrenees might be explained by both legacy (regional) and distant sources, in combination with environmental characteristics such as the presence of peatlands or streamwater physicochemistry, while MPs in fish are linked to recent local pollution sources such as single-use plastics. In contrast, MPs in biofilm matrix and sediments indicate a combination of distant (i.e., atmospheric deposition) and recent local pollution sources. Moreover, hydrodynamics and plastic density likely control MP distribution in rivers. Based on Fulton's condition factor, we also found that higher THg concentrations caused a negative impact on fish health (K < 1), while no impact of MPs could be seen. Therefore, we suggest that brown trout and biofilm can serve as bioindicators of atmospheric deposition of THg in high-altitude lakes and that biofilm is a reliable bioindicator to assess MP pollution in remote environments. Brown trout may also act as a bioindicator of MP pollution, but only efficiently in more polluted areas.

Effects of polyethylene terephthalate microplastics on cell growth, intracellular products and oxidative stress of Scenedesmus sp.

Polyethylene terephthalate (PET) has been widely utilized in the synthesis of textile materials and packaging of foods and beverages. In recent years, it has been commonly detected in the form of microplastics (MPs) in wastewater. However, the effects of PET MPs on microalgal intracellular products and their interrelationships have been poorly investigated. In this study, the microalgae Scenedesmus sp. Strain H-1 was exposed to PET MPs to explore their effects on the growth, intracellular products (such as lipids, carbohydrates, and proteins), and antioxidative defense systems of Scenedesmus sp. The results demonstrated that PET MPs significantly reduced Scenedesmus sp. cell growth, with a maximum inhibition rate of 38.25% in the 500 mg L−1 treatment group. PET MPs had negative effects on glucose and nitrate utilization rates and reduced intracellular carbohydrates, intracellular proteins, and photosynthetic pigments. Surprisingly, PET MPs reduced acetyl-CoA carboxylase activity but induced lipid accumulation in microalgae. In addition, PET MPs significantly decreased the essential linoleic acid concentration and increased the palmitic acid content, resulting in reduced biodiesel quality. PET MPs induced the production of reactive oxygen species and malondialdehyde as well as the activities of superoxide dismutase and catalase. The results of the PCA indicated that the response mechanism of Scenedesmus sp. to PET MPs exposure was synergistic. This study provides fundamental data on the impact of MPs on the intracellular products of microalgae.

The Impact of Microplastics on Global Food Production: A Brief Overview of This Complex Sector

Environmental pollution management combined with food safety represents two of the main challenges of the last decades. Soil and water contamination has historically threatened food safety. As ubiquitous pollutants, microplastics (MPs) have attracted increasing attention over the last few years. These particles can affect the balance of terrestrial, aquatic, and aerial ecosystems. Their negative impacts are intensified when they adsorb and carry toxic chemicals. They can circulate through organisms and accumulate in human beings via food and water. Physiological dysfunctions in all species continue to be reported, both in terrestrial and aquatic ecosystems. This article considers how this might be affecting the global production of food. It reports the adverse effects induced by MPs in soils, their properties and organisms growing within and upon them, including livestock and the pollinating agents necessary for plant growth. A separate section discusses the effects of MPs on aquaculture, mentioning effects on wild species, as well as farmed fish. The growing concern of the food production sector with MPs mimics that of the world with global warming; the danger is real and requires urgent attention.

A characterization and an exposure risk assessment of microplastics in settled house floor dust in Istanbul, Turkey.

The presence of microplastics in the indoor environment presents growing environmental and human health risks because of their physicochemical and toxic characteristics. Therefore, we aimed to isolate, identify, and characterize plastic debris in settled house floor dusts. This study is a rare study which assess the risks of plastic debris in settled house dust through multiple approaches including the estimated daily intake, pollution loading index, and polymer hazard index. The results indicated that polyethylene and polypropylene were the predominate polymer type of plastic debris in settled house dust with various shapes and colors. The risk assessment results also indicated the serious impact of microplastics in terms of extremely dangerous contamination as well as the fact that they present a polymer hazard. Results indicated that humans have a higher risk of exposure to microplastics via ingestion rather than inhalation. In addition, infants had a higher risk of potential intake compared to other age groups.

Machine learning prediction and interpretation of the impact of microplastics on soil properties

The annual microplastic (MP) release into soils is 4–23 times higher than that into oceans, significantly impacting soil quality. However, the mechanisms underlying how MPs impact soil properties remain largely unknown. Soil-MP interactions are complex because of soil heterogeneity and varying MP properties. This lack of understanding was exacerbated by the diverse experimental conditions and soil types used in this study. Predicting changes in soil properties in the presence of MPs is challenging, laborious, and time-consuming. To address these issues, machine learning was applied to fit datasets from peer-reviewed publications to predict and interpret how MPs influence soil properties, including pH, dissolved organic carbon (DOC), total P, NO3−-N, NH4+-N, and acid phosphatase enzyme activity (acid P). Among the developed models, the gradient boost regression (GBR) model showed the highest R2 (0.86–0.99) compared to the decision tree and random forest models. The GBR model interpretation showed that MP properties contributed more than 50% to altering the acid P and NO3−-N concentrations in soils, whereas they had a negligible impact on total P and 10–20% impact on soil pH, DOC, and NH4+-N. Specifically, the size of MPs was the dominant factor influencing acid P (89.3%), pH (71.6%), and DOC (44.5%) in soils. NO3−-N was mainly affected by the MP type (52.0%). The NH4+-N was mainly affected by the MP dose (46.8%). The quantitative insights into the impact of MPs on soil properties of this study could aid in understanding the roles of MPs in soil systems.

Trophic transfer and their impact of microplastics on estuarine food chain model

Microplastic contamination in marine ecosystems, and its negative effects through trophic transfer among marine organisms, remains a growing concern. Our study investigates the trophic transfer and individual impacts of polystyrene microplastics (MPs) in an estuarine food chain model, comprising Artemia salina as primary organism, Litopenaeus vanamei as secondary organism, and Oreochromis niloticus as tertiary organism. A. salina were exposed to 1 µm polystyrene microplastics (106 particles/ml), further it was fed to L.vannamei, which, in turn, were fed to O.niloticus. MPs transfer was studied over 24 and 48 h. Fluorescence microscopy confirmed MPs presence in the gut and fecal matter of all the test organisms. Histopathology revealed MPs in the gut epithelium, but did not translocate to other tissues of the test species. MPs exposed A.salina had a bioconcentration factor of 0.0029 ± 0.0008 (24 h) and 0.0000941 ± 0.0000721 (48 h). Whereas, the bioaccumulation factor values for L. vanamei were 0.00012143 ± 0.000009 (24 h) and 0.0025899 ± 0.0024101 (48 h), and for O.niloticus were 0.154992 ± 0.007695 (24 h) and 0.00972577 ± 0.00589923 (48 h). Despite low MPs transfer among trophic levels, the induced stress was evident through biochemical responses in all the test species. This implies the potential risk of MPs ultimately reaching humans via the food chain.

Uncovering the intricate relationship between plant nutrients and microplastics in agroecosystems

Recent scientific and media focus has increased on the impact of microplastics (MPs) on terrestrial and soil ecosystems. However, the interactions between MPs with macronutrients and micronutrients and their potential consequences for the agroecosystem are not well understood. Wheat (Triticum aestivum) is a staple food grown globally and has special importance for nations economies. Different elements can cause dangerous outcomes for wheat quality and production yield. In this study, batch adsorption experiments were done using 1 g of polyethylene tetra phthalate MP particles (PET-MPs) in varying concentrations of thirteen elements. The adsorption data were fitted by two common adsorption models (Langmuir and Freundlich). The effect of pH on the speciation of elements in aqueous solutions was investigated. The non-invasive characterization methods indicate the importance of O- and H-containing groups as the main component of selected MPs in controlling the adsorption of the elements ions. In the current study, adsorption and potential transport of the adsorbed macronutrients (K and Na) and micronutrients (Ni, Co, Cu, Al, Ba, Se, Fe, As, B, V and Ag) which include some beneficial (Na, Se, V), and non-essential or toxic elements (Al, As, Ag, Ba) onto MPs to the simulated roots of wheat were evaluated. The maximum sorption capacities of K+> Ni+2> Na+ > Co2+> Cu2+>Al+3 >Ba+2 >Se4+>Fe2+ >As5+ >B3+ >V5+> Ag + on PET-MPs at pH 5.8 and 25 ± 1 °C were 290.6 > 0.52> 0.51 > 0.20> 0.10 > 0.051> 0.024 > 0.003> 0.003 > 0.0015> 5.05 × 10−4> 1.7 × 10−4>3.7 × 10−6 mg g−1, respectively. The results highlight the importance of PET-MPs in controlling element adsorption in the rhizosphere. Our observations provide a good start for understanding the adsorption of multiple elements from the soil rhizosphere zone by PET-MPs.

Effect of chronic and humped input of polystyrene microplastics on rice growth

The impact of microplastics (MPs) pollution on farmland is influenced by the type of rainfall and the hydrography of the site. However, it is not clear how the arrival of MPs to farmland through different input modes will affect plant growth. Here, we simulated the effects of polystyrene (PS) MPs chronic input (50 mg/L), humped input (0, 0, 0, 100, 250, 100, 0, 0, 0 mg/L) and without PS MPs input (control) on rice growth through hydroponic experiment. The results showed that the input mode of MPs had a significant effect on the growth of rice. Compared with the control, the chronic input reduced the fresh weight of rice roots, stems and leaves by 61 %, 60 % and 64 %, respectively. Humped input only had a significant effect on the fresh weight of rice roots and stems, which were reduced by 24 % and 17 %, respectively. Both PS MPs input modes significantly reduced the chlorophyll content of plants. However, the chronic input of PS MPs also stimulated the antioxidant system of rice, improved peroxidase (POD) activity, and changed the nutrient content in plant tissues. Overall, chronic input of PS MPs had a greater impact on rice growth. Therefore, it is crucial to consider the input modes of MPs when assessing their effects on plant growth. This study is the first to investigate the effects of different input modes of MPs on plant growth, providing new insights into the pathways through which MPs affect plants.

Microcosm study of the effects of polyester microfibers on the indigenous marine amphipod (Cyphocaris challengeri) in the Strait of Georgia (BC, Canada)

Microplastics (MP) remain contaminants of great concern in the ocean because of their abundance, prevalence, and threat to marine organisms. Still, there is a great need for studies on the impact of MP on marine zooplankton. Here, we investigated the effects of polyethylene terephthalate (PET) microfibers (Mf) on the survival, Mf ingestion and retention, predation, and fecal pellets (FP) of the marine amphipod (Cyphocaris challengeri) at environmentally relevant concentrations (0, 10, 100, 1000, 10,000 and 50,000 Mf·L−1) and varied exposure time (24, 48 and 72 h). Our study demonstrated that exposure of C. challengeri to PET Mf did not affect their survival. The average number of ingested Mf and the Mf ingestion rate increased significantly with Mf concentrations. Nonetheless, the Mf ingestion rates by C. challengeri decreased significantly between 24 and 72 h in the two highest Mf treatments (10,000 and 50,000 Mf·L−1), suggesting careful rejection of the Mf or reduced feeding activity. Indeed, PET Mf significantly reduced the copepod feeding rate of the amphipods at Mf concentrations ≥1000 Mf·L−1 after 24 and 48 h of exposure duration. Over time, prey intake reduction in amphipods due to Mf ingestion could affect their reproductive outcome, growth, development, and cellular and ecosystem function. The encapsulation of PET Mf into the FP of C. challengeri significantly increased the FP density and sinking velocities, ultimately doubling the transfer rate of the FP from the surface waters to the sediments in SoG. Conversely, ingesting PET microfibers and their incorporation in FP will potentially enhance the role of C. challengeri in the biological C pump and sequestration in SoG. Our study showed that changes in Mf concentration had a more significant effect on C. challengeri Mf ingestion and ingestion rate, prey consumption, FP density and sinking velocity than the exposure time.

Palladium-doped and undoped polystyrene nanoplastics in a chronic toxicity test for higher plants: Impact on soil, plants and ammonium oxidizing bacteria

There is a lack of knowledge about the fate and impact of microplastics (MPs) and nanoplastics (NPs), as well as their potential uptake and impact on plants and microorganisms. The predicted environmental concentrations (PEC) of frequent polymers in soils are low, and therefore, difficult to detect with the available techniques, which explains the knowledge gaps. Therefore, model particles (polystyrene particles (PS-P), 343 nm) and palladium (Pd) nanoparticle-doped polystyrene particles (PS-Pd-PS-P, 442 nm) were synthesized, characterized, and subsequently applied to agricultural soils (Cambisol, Podzol, PS target contents: 25 mg kg−1, 75 mg kg−1, 225 mg kg−1). A combination of different techniques, such as inductively coupled plasma-mass spectrometry (ICP-MS), pyrolysis-gas chromatography–mass spectrometry (Pyr-GC–MS), dynamic light scattering (DLS), and scanning electron microscopy (SEM), were used to characterize the particles in the dispersions, soils and plants. The spiked soils were applied to a chronical plant toxicity test with oat (Avena sativa). The applied particle contents could be recovered from both soils by ICP-MS (Pd, 89% - 99%), and Pyr-GC–MS (PS, 73% - 120%). Moreover, non-aggregated particles in soils and on oat roots were visualized through SEM. The ratio obtained for the Pd contents in oat roots to that in the Cambisol (2.2–2.7) and the Podzol (2.3–2.6) implied that particles accumulated on the root surface or in the roots. No Pd was detected in the oat shoots, which indicated that no translocation occurred from the roots to the shoots. Despite particle accumulation at or in the roots, no clear effects on plant growth were observed. Furthermore, the soil microorganisms (Podzol) and the soil water repellency (Cambisol, Podzol) showed no clear monotone concentration-response relationship after exposure to PS-P and PS-Pd-PS-P. The findings are complex and illustrate the urgent need for further sophisticated experimental studies to elucidate the impacts of NPs on physicochemical soil function, plants, and soil organisms. The model PS-P doped with Pd nanoparticles significantly enhanced the development and validation of methods for investigating MPs and NPs in environmental matrices, highlighting their considerable potential for further studies.

Effect and mechanism of microplastics exposure against microalgae: Photosynthesis and oxidative stress

The occurrence of microplastics (MPs) within aquatic ecosystems attracts a major environmental concern. It was demonstrated MPs could cause various ecotoxicological effects on microalgae. However, existing data on the effects of MPs on microalgae showed great variability among studies. Here, we performed a meta-analysis of the latest studies on the effects of MPs on photosynthesis and oxidative stress in microalgae. A total of 835 biological endpoints were investigated from 55 studies extracted, and 37 % of them were significantly affected by MPs. In this study, the impact of MPs against microalgae was concentration-dependent and size-dependent, and microalgae were more susceptible to MPs stress in freshwater than marine. Additionally, we summarized the biological functions of microalgae that are primarily affected by MPs. Under MPs exposure, the content of chlorophyll a (Chl-a) was reduced and electron transfer in the photosynthetic system was hindered, causing electron accumulation and oxidative stress damage, which may also affect biological processes such as energy production, carbon fixation, lipid metabolism, and nucleic acid metabolism. Finally, our findings provide important insights into the effects of MPs stress on photosynthesis and oxidative stress in microalga and enhance the current understanding of the potential risk of MPs pollution on aquatic organisms.

The Impact of Micro- and Nanoplastics on Aquatic Organisms: Mechanisms of Oxidative Stress and Implications for Human Health—A Review

In recent years, interest in the impact of environmental pollutants on the ecosystem has increased significantly, with particular attention being paid to the relationship between climate change and the aquatic world. This is because increasing pollution is causing fundamental changes to the welfare of animals and the marine ecosystem. A primary focus is on the impact of microplastics (MPs) and nanoplastics (NPs), as evidenced by our bibliometric network analysis (BNA). However, while research is focused on the accumulation of these pollutants in aquatic organisms, their effects on redox homeostasis are still seldom discussed despite the role played by reactive oxygen species and mitochondrial well-being in maintaining an optimal state of health. However, some scientific evidence suggests that the accumulation of MPs and NPs in organisms at the base of the trophic chain can cause a transfer of these substances towards more complex organisms, reaching humans through the consumption of aquatic fauna as food. Therefore, in this review, we have tried to discuss the effects of these substances on oxidative stress in aquatic organisms, even if studies in this regard are still scarce.

Unveiling the impact of short-term polyethylene microplastics exposure on metabolomics and gut microbiota in earthworms (Eudrilus euganiae)

Microplastics (MPs) pose a significant environmental concern, particularly for terrestrial fauna. In this study, earthworms were used as a model organism to investigate the ecotoxicological effects of short-term exposure to virgin MPs on changes in metabolome and gut microbiota. High-throughput untargeted metabolomics showed significant internal reactions in the earthworms' metabolic processes due to MPs exposure, even when no visible stress signs, such as changes in growth or mortality rates, were present. Earthworms exposed to different concentrations of polyethylene (PE) MP exhibited significant disruption in 39 and 199 molecular features related to energy and lipid metabolism, anti-inflammatory, cell signaling, and membrane integrity. The activities of enzymes and transport proteins in earthworms were dysregulated when exposed to PE. Changes in the gut microbiota's community structure and complexity were observed in response to PE MPs exposure. Despite the relative stability in alpha-diversity and relative abundance, shifts in beta-diversity and network analysis in the PE-exposed group were indicative of an adaptive response to MPs. Earthworms exhibited resilience or adaptation in response to MPs exposure, potentially maintaining their functionality. This study provides preliminary insights into the impact of MPs on soil invertebrates like earthworms and highlights the need for further exploration of long-term effects and underlying molecular mechanisms.

Effects of Microplastics on Soil Carbon Mineralization: The Crucial Role of Oxygen Dynamics and Electron Transfer.

Although our understanding of the effects of microplastics on the dynamics of soil organic matter (SOM) has considerably advanced in recent years, the fundamental mechanisms remain unclear. In this study, we examine the effects of polyethylene and poly(lactic acid) microplastics on SOM processes via mineralization incubation. Accordingly, we evaluated the changes in carbon dioxide (CO2) and methane (CH4) production. An O2 planar optical sensor was used to detect the temporal behavior of dissolved O2 during incubation to determine the microscale oxygen heterogeneity caused by microplastics. Additionally, the changes in soil dissolved organic matter (DOM) were evaluated using a combination of spectroscopic approaches and ultrahigh-resolution mass spectrometry. Microplastics increased cumulative CO2 emissions by 160-613%, whereas CH4 emissions dropped by 45-503%, which may be attributed to the oxygenated porous habitats surrounding microplastics. Conventional and biodegradable microplastics changed the quantities of soil dissolved organic carbon. In the microplastic treatments, DOM with more polar groups was detected, suggesting a higher level of electron transport. In addition, there was a positive correlation between the carbon concentration, electron-donating ability, and CO2 emission. These findings suggest that microplastics may facilitate the mineralization of SOM by modifying O2 microenvironments, DOM concentration, and DOM electron transport capability. Accordingly, this study provides new insights into the impact of microplastics on soil carbon dynamics.

Microplastics in waste management systems: A review of analytical methods, challenges and prospects

Numerous studies have reported the presence of microplastics (MPs) in waste collection and disposal systems. However, current scientific studies on measuring MP occurrence in a waste management context are not comparable due to a lack of standardized methodologies. Consequently, the impact of MPs on ecosystems and human health remains largely unclear. To address the inconsistencies, present in published studies, this review thoroughly examines sample preparation techniques for transfer stations, landfill leachate, recycling, compost, and incineration ash samples. Furthermore, various analytical approaches such as flotation, filtration, and organic matter digestion, as well as morphological categorization, identification, and quantification, are subsequently rigorously assessed. The benefits and limitations of each methodology are evaluated to facilitate the development of accurate and effective methods for detecting and characterizing nanoplastics. Recent research suggests that plastic recycling and composting facilities are the primary environmental sources of microplastic pollution among different waste treatment methods. The most prevalent microplastic types discovered in waste management were polyethylene (PE) and polypropylene (PP), with fragment and fiber being the most frequently reported morphologies. The review highlights a number of tactics that could be integrated into the methodology development for detecting microplastics in waste management systems (WMS), ultimately leading to better consistency and reliability of data across different studies. In essence, this will advance our comprehension of potential risks associated with microplastics.

Shifting enzyme activity and microbial composition in sediment coregulate the structure of an aquatic plant community under polyethylene microplastic exposure

It has been shown that microplastics (MPs) interfere with critical biological processes (including development, growth and fitness); however, there is no information about the impact of MPs on plant productivity and community structure in freshwater ecosystems. Here, we investigated the effects of two sizes (MIC: 20–300 μm, MAC: 2–3 mm) and three concentrations (0.03 %, 0.3 %, and 0.6 %) of low-density polyethylene MPs on submerged plant communities. The results showed that plant responses to MPs were species specific, which can affect plant community structure. For canopy-forming species (Hydrilla verticillata), total biomass increased by 4 %–46 % and relative abundance increased by 23 %–34 % under MP exposure, while rosette-forming species (Vallisneria natans) decreased by 44 %–67 % in total biomass and relative abundance decreased by 54 %–71 %. Myriophyllum spicatum growth was largely unaffected by MPs. Community diversity was negatively correlated with MAC treatments, and the community root to shoot ratio decreased by 40 %, while community productivity increased by 41 % at a 0.6 % MAC concentration. Although MPs did not change the microbial community composition, alpha diversity was reduced at the 0.6 % concentration. It is worth noting that 0.6 % is a higher concentration than most field sediment investigations. During the experiment, the activity of functional enzymes related to carbon and nitrogen increased under most MP treatments. Structural equation modelling showed that MIC changed the community structure mainly by driving sediment enzyme activity, while MAC changed the community structure mainly by driving plant growth. The results implied that MPs may affect sediment enzymatic activities, microbial alpha diversity and aquatic plant growth, potentially altering the diversity and stability of aquatic ecosystems.

Impact of soil structure and texture on occurrence of microplastics in agricultural soils of karst areas

The impact of microplastics (MPs) on soil ecosystems has attracted widespread attention; however, the effects of soil structure and texture on the occurrence of MPs are not fully understood. In this study, we investigated the effects of soil structure and texture on the abundance of MPs and their potential mechanisms in agricultural soils of karst areas in Guizhou, China. The results showed the average abundance of MPs was 2948 items/kg. The soil texture in the study area can be categorized into seven types such as powdered-light clay, the range of total soil porosity was 39.05–69.22 % and the range of soil bulk density was 0.66–1.51 g/cm3. Soils with a powdered-light clay, low soil porosity, and low soil bulk density showed higher MPs pollution. The percentage of pellet MPs in agricultural soils with a powdered-light clay was 84 %, which was higher than that of the other soil textures. The direct effects of soil texture, soil porosity, and soil bulk density on MPs abundance were much lower than the indirect effects, with soil texture having the highest effect on MPs abundance. We speculated that karst geology may affect the accumulation and distribution of MPs in soil by affecting soil texture and structure, which, in turn, affects the fragmentation and migration of MPs. These findings will help to better understand the mechanisms of soil MPs pollution and provide a scientific basis for the development of relevant control strategies.