Impact Of Regulation Research Articles

Role of Ethylene in the Regulation of Plant Developmental Processes

Ethylene, a gaseous phytohormone, is emerging as a central player in the intricate web of plant developmental processes from germination to senescence under optimal and stressed conditions. The presence of ethylene has been noted in different plant parts, including the stems, leaves, flowers, roots, seeds, and fruits. This review aims to provide a comprehensive overview of the regulatory impact of ethylene on pivotal plant developmental processes, such as cell division and elongation, senescence, abscission, fruit and flower development, root hair formation, chloroplast maturation, and photosynthesis. The review also encompasses ethylene biosynthesis and signaling: a snapshot of the regulatory mechanisms governing ethylene production. Understanding of the impact of ethylene’s regulatory functions on plant developmental processes has significant implications for agriculture, biotechnology, and our fundamental comprehension of plant biology. This review underscores the potential of ethylene to revolutionize plant development and crop management.

Construction of MoB@LDH heterojunction and its derivates through phase and interface engineering for advanced supercapacitor applications

Layered double hydroxide (LDH) has been attracted widespread attention in supercapacitor due to their unique layered structure and associated advantages. However, the inherent limitations of low electrical conductivity and reaction kinetics rate of LDH restrict its widespread application. Various modification techniques, such as heterojunction formation, phosphorization and introduction of phosphorus vacancies, are employed to modify LDH with the goal of improving the electrochemical performance. Preparation of composite materials using MoB MBene as conductive template and phosphorization are the effective ways for enhancing the electrical conductivity of electrode materials. MoB MBene is prepared using a modified method that combines NaOH etching and a high-temperature hydrothermal process. The presence of phosphorus vacancy is beneficial for enhancing the kinetics rate during electrode reactions. Through the synergistic effect of various modification methods, MP2 demonstrates an optimal electrochemical performance with a superior specific capacitance of 1731.19F/g (238.28 mAh g−1) at 1 A/g. It also demonstrates an impressive rate capacity of 81.28 % at 10 A/g and maintains a satisfactory capacitance retention of 88.14 % after 5000 cycles. In addition, a fabricated MP2//AC ASC device achieves an impressive energy density of 39.91 Wh kg−1 at the power density of 948.25 W kg−1 and demonstrates satisfactory cycling stability of 78.76 % after 5000 cycles. This work presents a comprehensive framework for analyzing the impact of material structure, components, and crystal phases on energy storage performance. It also examines the regulatory impact of different modification methods on energy storage mechanisms.

The Application of the Capital Asset Pricing Model (CAPM) in China's Financial Market: An Analysis of Regulatory Impact and International Comparison

Indentation of Abstract should be left 0.53"/1.35cm. As the world's second largest economy, China is also one of the world's biggest emerging economies; The Capital Asset Pricing Model (CAPM) is one of the most classic pricing models, and China's asset pricing model has gained a lot of experience from it. This research investigates the application of the CAPM in China's financial market, focusing on the influence of financial market regulation and conducting a comparative analysis with international markets. CAPM is a fundamental asset pricing model used to estimate asset expected returns and risks based on their covariance with the overall market portfolio. The study aims to analyze the practical implementation of CAPM in China's financial market, considering the impact of financial market regulation on the model's effectiveness. Key variables such as market data, risk-free rates, and stock returns are utilized, with specific adjustments made to accommodate China's unique regulatory environment. The research's significance lies in providing valuable insights into asset pricing and risk assessment in China's financial market, as well as implications for investors and policymakers. By comparing the results with international markets, this study contributes to a broader understanding of the CAPM model's applicability and effectiveness in diverse regulatory settings.

Transcription factors and miRNA act as contrary regulators of gene expression in the testis and epididymis of Bos indicus animals

Spermatogenesis is highly conserved among mammalians, but its gene expression and regulatory profile are not entirely known. As transcription factors (TFs) and miRNAs are crucial for gene expression regulation, identifying genes negatively regulated by miRNAs and positively regulated by TFs in the testis and epididymis can provide a deeper understanding of gene expression and regulatory patterns. To do this, we used expression data coming from RNA-Seq and miRNA-Seq experiments made with biopsies from testicular parenchyma, head of the epididymis, and tail of the epididymis of four Brahman bulls. We identified miRNA differentially expressed (DE) by comparing the three distinct tissues. A co-expression analysis combined with a regulatory impact factor approach identified miRNAs and TFs with regulatory impact over gene expression regulation in the Bos indicus tissues studied. We identified 116 DE miRNAs, 206 miRNAs and 237 TFs with a significant regulatory impact on mRNA patterns in the tissues’ comparisons. bta-miR-196b was the only DE miRNA for all tissue comparisons and it may be a regulator of spermatogenesis through its links with adipogenesis and insulin biosynthesis. DE genes and TFs involved in contrary regulations between the epididymis head and testis parenchyma were associated with spermatogenesis, sexual reproduction, and sperm motility. Our results provide possible mechanisms, governed by the contrary effect of miRNA and TF, leading to the differential expression between the studied tissues. We have demonstrated that our predictions of miRNAs and TFs co-regulations over target DE genes can retrieve known regulatory mechanisms and predict new ones that merit further validation.

Bovine parainfluenza type 3 virus induces incomplete autophagy to promote viral replication by activated beclin1 in vitro

Bovine Parainfluenza virus Type 3 (BPIV3) is one of the most important pathogens in cattle, capable of causing severe respiratory symptoms. Numerous studies have shown that autophagy plays a diverse role in the infection process of various pathogens. The influence of autophagy machinery on BPIV3 infection has not yet been confirmed. In the present study, we initially demonstrated that the expression of LC3 was significantly increased and exhibited a notable increase in double or single-membrane vesicles under a transmission electron microscope during BPIV3 infection. These observations unequivocally establish the induction of steady-state autophagy in vitro consequent to BPIV3 infection. Furthermore, quantification of autophagic flux substantiates the induction of an incomplete autophagic process during BPIV3 infection. Additionally, through targeted interventions, we demonstrate the regulatory impact of pharmacological agents influencing autophagy and RNA interference targeting an autophagy-associated protein on viral replication. Intriguingly, our data revealed that BPIV3 infection enhanced the phosphorylation of rapamycin kinase (mTOR). This result demonstrated that mTOR does not operate as a counteractive regulator of BPIV3-induced autophagy. Instead, we discern an augmentation in the expression of Beclin1, a key autophagy initiator, which complexes with Vps34, constituting a Class III phosphatidylinositol 3-kinase. This phenomenon serves as a hallmark in the inaugural phase of autophagy initiation during BPIV3 infection. Collectively, these discernments underscore that BPIV3 infection actively stimulates autophagy, thereby enhancing viral replication through the activation of Beclin1, independently of the mTOR signaling pathway. This nuanced comprehension significantly contributes to unraveling the intricate molecular mechanisms governing BPIV3-induced autophagy.

What we must be: AI and the future of porn studies

ABSTRACT This article explores the potential impacts of artificial intelligence (AI) on the future of pornography and pornography studies. It links the content policies created by technology companies such as OpenAI, which prohibit sexually explicit material, to the social media, technology, and entertainment industries before them, and examines the cultural and regulatory impact on pornography, sexuality, and human relationships. Drawing on the work of Joseph Weizenbaum, the author argues that the increasing disconnection of humanity from creative expression, along with growing disinterest in the consequences of that disconnection, will have profound consequences for the future of pornography studies.

Consumer Trust in Digital Banking: A Qualitative Study of Legal and Regulatory Impacts

The shift towards digital banking has significantly transformed the financial landscape, necessitating a deeper understanding of the factors influencing consumer trust. This study aims to explore how legal and regulatory frameworks, coupled with perceived security measures, impact consumer trust in digital banking services. A qualitative research design was employed, using semi-structured interviews to gather data from 30 participants who use digital banking services. Participants were selected through purposive sampling to cover a broad demographic range. Data were collected to achieve theoretical saturation and analyzed using NVivo software to identify emerging themes and patterns. The analysis revealed three main themes: Regulatory Impact, Perceived Security, and Consumer Trust Dynamics. Regulatory Impact included categories such as Compliance Standards, Consumer Protection Policies, Transparency, and Enforcement and Penalties. Perceived Security was detailed through Technological Safeguards, Risk Management, User Experience, and Data Management. Consumer Trust Dynamics comprised Initial Trust Formation, Trust Maintenance, Impact of Negative Experiences, Role of Peer Influence, and Long-term Relationship Building. The study concluded that a robust legal and regulatory framework, effective security measures, and dynamic trust-building practices are pivotal in fostering consumer trust in digital banking. Transparent regulations, advanced security technologies, and proactive customer relationship management are essential for banks to maintain and enhance trust among users.

Childhood adversity and depression of older adults: the moderating effect of social participation.

Examine the effect of childhood adversity on depression in older adults and the regulatory impact that social participation has on depression. Based on 6,704 standard-compliant research subjects, single factor analysis, multiple linear regression model, and tendency score matching were used to analyze the impact of childhood adversity on depression in older adults and the regulatory effect of social participation. The depression rate is higher among women, young age, low education, unmarried, in agricultural households, older adults with low annual income, pre-retirement work type in agriculture, non-drinking, and those with two or more chronic diseases (p < 0.05). Children who experienced adversity as children are more likely to suffer from depression as adults (β = 0.513, 0.590, 0.954, 0.983, 1.221, 0.953, 0.718; p < 0.05). Through the tendency score, the result is matched with the endogenous test. As well, older adults are more likely to suffer psychological damage from a greater number of childhood adversities in their early years (β = 1.440, 2.646, 4.122; p < 0.001). It has been shown that social participation will reduce the negative impact of low-income family economic circumstances on depression among older adults of all ages (β = -0.459,-0.567; p < 0.01), aggravate depression resulting from "neighborhood void of mutual assistance" and "no more fun to play" for older adults of all ages (β = 1.024, 0.894; p < 0.01), and exacerbate depression resulting from "loneliness because there are no friends" for the oldest old (β = 0.476, 0.779; p < 0.05). Older adults who experience childhood adversity are more likely to suffer from depression. Social participation plays a regulatory role in the relationship between childhood adversity and depression in older adults. For older adults' mental health to improve, family and social adversity should be prevented, and moderate participation in society should be encouraged.

MiRNAs and NFKB1 and TRAF6 target genes: The initial functional study in CD14+ monocytes in rheumatoid arthritis patients.

We predicted miRNAs with regulatory impact on NFKB1 and TRAF6 gene expression and selected the miR-194-5p, miR-124-3p, miR-9-5p, and miR-340-5p and their target genes for expression analyses on CD14+ monocytes from rheumatoid arthritis (RA) patients and healthy controls. Additionally, we evaluated the influence of genes and miRNA expression on RA patients' cytokine levels. No difference was observed in genes or miRNAs expression when compared to healthy controls and RA patients or clinical parameters. However, we found a significant difference between miR-194-5p and miR-9-5p levels (FC=-2.31; p=0.031; FC=-3.05;p=0.031, respectively) and non-prednisone users as compared to prednisone using patients. We conducted correlation analyses to identify the strength of the relationship between expression data and cytokine plasma levels. We observed a moderate positive correlation between miR-124-3p expression and IL-6 plasma levels (r=0.46; p=0.033). In addition, overexpression of miRNAs was concomitant to TRAF6 and NFKB1 genes as indicated by correlation analyses: TRAF6 and miR-194-5p (r=0.60;p<0.001) and miR-9-5p (r=0.63;p<0.001) and NFKB1 and miR-194-5p (r=0.72;p<0.001), miR-9-5p (r=0.72;p<0.001) and miR-340-5p (r=0.61;p<0.001). NFKB1 and TRAF6 genes and miRNAs monocyte expression do not appear to be related to RA but showed a significant difference in different groups of RA therapy. In addition, increased levels of miRNAs can be linked to concomitant overexpression of TRAF6 and NFKB1 in monocytes and act as its regulators.

Serum melatonin levels in type 2 diabetic patients with depressive symptoms compared to non-depressed individuals.

Melatonin, mainly regulating the body's circadian rhythm, may have protective effects against type 2 diabetes mellitus (DM2)-induced depression due to its antioxidant and regulatory impact in the pathogenesis of both DM2 and depression. This study aimed to find the association of serum melatonin levels with depression in DM2 patients. A total of 50 DM2 patients were recruited in this retrospective cross-sectional study and divided into 25 patients with depression (DM2-DP) and 25 without depression symptoms (DM2-NDP). Depression was diagnosed using the Hospital Anxiety and Depression Scale (HADS) assessment. Fasting blood samples were collected and examined for the level of serum melatonin and other biomarkers. All statistical analysis was performed by SPSS software Version 22, and a p-value less than 0.05 was considered statistically significant for all tests. The depression score was significantly lower in DM2-NDP than DM2-DP (p< 0.001). The mean weight was significantly lower in the DM2-DP group (P= 0.021). Total cholesterol, triglyceride, and anxiety scores were higher, and the melatonin level was lower in DM2-DP. The correlation of melatonin levels was positive with age, DBP, HbA1C, FBS, and TG. In contrast, it was negative with male gender, BMI, diabetes duration, SBP, TC, family history of DM, depression score, and anxiety score. However, no significant differences were seen. Lower melatonin may be associated with depression and anxiety in patients with DM2. The serum melatonin level might be a strong predictor of depression in DM2 patients.

Regulatory impact on Quality of Electricity Distribution Services: The case of Latin America and the Caribbean

Regulatory impact on Quality of Electricity Distribution Services: The case of Latin America and the Caribbean

EX-POST ANALYSIS AS AN INTEGRAL PART OF THE LEGISLATIVE DECISION-MAKING PROCESS

The paper presents the theoretical arguments and practical benefits of developing a system of ex-post evaluation of regulatory impact by studying the actual effects of the decisions of legislative decision-making bodies. The individual issues under consideration involve: the analysis of general assumptions of the decision-making approach in legal studies and its application to the problems of a law-making policy; presentation of the stages of the legislative decision-making process, with a particular emphasis placed on the effects of such decisions; and discussion of the conditions of a contemporary law-making policy, from both the theoretical and practical points of view, based on a distinction of several dimensions of legislative decision-making (instrumental, axiological, power game, social conflict, discursive, social normative system, economic, and temporal). For each dimension, ex-post evaluation criteria are proposed.

Reconciling Regulatory Impact Analyses and Agencies' Statutory Mandates for Environmental Regulations under Loper Bright

Reconciling Regulatory Impact Analyses and Agencies' Statutory Mandates for Environmental Regulations under Loper Bright

GARNL3 identified as a crucial target for overcoming temozolomide resistance in EGFRvIII-positive glioblastoma.

Amplification of the epidermal growth factor receptor (EGFR) and its active mutant type III (EGFRvIII), frequently occurr in glioblastoma (GBM), contributing to chemotherapy and radiation resistance in GBM. Elucidating the underlying molecular mechanism of temozolomide (TMZ) resistance in EGFRvIII GBM could offer valuable insights for cancer treatment. To elucidate the molecular mechanisms underlying EGFRvIII-mediated resistance to TMZ in GBM, we conducted a comprehensive analysis using Gene Expression Omnibus and The cancer genome atlas (TCGA) databases. Initially, we identified common significantly differentially expressed genes (DEGs) and prioritized those correlating significantly with patient prognosis as potential downstream targets of EGFRvIII and candidates for drug resistance. Additionally, we analyzed transcription factor expression changes and their correlation with candidate genes to elucidate transcriptional regulatory mechanisms. Using estimate method and databases such as Tumor IMmune Estimation Resource (TIMER) and CellMarker, we assessed immune cell infiltration in TMZ-resistant GBM and its relationship with candidate gene expression. In this study, we examined the expression differences of candidate genes in GBM cell lines following EGFRvIII intervention and in TMZ-resistant GBM cell lines. This preliminary investigation aimed to verify the regulatory impact of EGFRvIII on candidate targets and its potential involvement in TMZ resistance in GBM. Notably, GTPase Activating Rap/RanGAP Domain Like 3 (GARNL3) emerged as a key DEG associated with TMZ resistance and poor prognosis, with reduced expression correlating with altered immune cell profiles. Transcription factor analysis suggested Epiregulin (EREG) as a putative upstream regulator of GARNL3, linking it to EGFRvIII-mediated TMZ resistance. In vitro experiments confirmed EGFRvIII-mediated downregulation of GARNL3 and decreased TMZ sensitivity in GBM cell lines, further supported by reduced GARNL3 levels in TMZ-resistant GBM cells. GARNL3 downregulation in EGFRvIII-positive and TMZ-resistant GBM implicates its role in TMZ resistance, suggesting modulation of EREG/GARNL3 signaling as a potential therapeutic strategy.

ОЦЕНКА РЕГУЛИРУЮЩЕГО ВОЗДЕЙСТВИЯ В СИСТЕМЕ ГОСУДАРСТВЕННОГО УПРАВЛЕНИЯ РОССИЙСКОЙ ФЕДЕРАЦИИ: ТЕКУЩАЯ СИТУАЦИЯ И ПУТИ РАЗВИТИЯ

The article presents an analysis of the use of the regulatory impact assessment (RIA) procedure in the constituent entities of the Russian Federation. This procedure became mandatory for use in Russian regions in 2014 in order to improve the quality of regulations governing business and investment activities before they are adopted. The author identified ways to increase the efficiency of the regulatory impact assessment procedure, the main characteristics of options for using the procedure in different regions, the existing difficulties in its implementation, and presented possible ways to increase the efficiency of using ODS at the regional level.

Wykorzystanie Oceny Skutków Regulacji przez uczestników procesu konsultacji

The aim of the article is an attempt to present the legal and administrative potential of the Regulatory Impact Assessment (RIA) from the point of view of social influencing during the consultation of draft legal solutions. It is a tool intended to support decision-makers in the decision-making process regarding the implementation of public policies, which allows them to base these decisions on the so-called evidence (evidence-based approach). RIA is conducted by officials and constitutes a separate part of the justification for a draft normative act in the Polish government legislative process. However, taking into account the scope of information required, as well as the stages of the legislative process, it may constitute an important tool for stakeholders through which – based on evidence – it is possible to influence the law being adopted. The article presents the results of an analysis of the extent to which the social side uses this opportunity.

BEYOND THE COURTROOM: THE EVOLUTION OF RIGHTS-BASED CLIMATE LITIGATION FROM URGENDA TO HELD AND ITS POLICY IMPACT

This paper examines the growing trend of employing international and human rights law in domestic climate change lawsuits as a strategic tool for legal action. Using a qualitative and comparative analysis of seminal case law, such as Urgenda Foundation v the Netherlands and R (Friends of the Earth and others) v Heathrow Airport Ltd and Held v State, this paper aims to explain the juridical trajectories and methodologies employed in rights-based climate litigation. The primary objective is to critically evaluate the potential regulatory impact of this emergent jurisprudential paradigm on both domestic legislation and international climate change treaties. The study posits that when traditional enforcement mechanisms enshrined in international environmental law treaties prove ineffective, domestic litigation grounded in human rights claims serve as a catalyst for transformative jurisprudence. This, in turn, can exert substantial pressure on state and non-state actors, compelling them to adopt more strict regulatory measures to mitigate the effects of climate change. The aim of this paper is twofold: first, to elucidate the effectiveness and development of rights-based jurisprudence in climate litigation, and second, to assess its potential for influencing the creation of stronger regulatory mechanisms at both state and international levels. The paper argues that when international treaties fail to take adequate climate change action, domestic lawsuits based on human rights claims start to serve as a lever of change, pressuring both state and non-state actors into adopting more ambitious measures. The study underscores the importance of this rights-based approach not merely as a legal strategy but as a multifaceted tool for effectuating systemic regulatory advancements and fostering climate justice.

Regulatory impact assessment for law reform: A comparison of the parliament role

Regulatory impact assessment (RIA) is a pivotal tool for shaping and enhancing the quality and effectiveness of laws (Davidson et al., 2021). This research focuses on comparing RIA mechanisms in the Organisation for Economic Co-operation and Development (OECD) member countries and Thailand. It involves analyzing RIA criteria, methodologies, impact assessment report preparation, and quality assurance for legislation. Employing qualitative research methods, the study employs document analysis, in-depth interviews with key informants from legislative, administrative, and legal sectors, individuals impacted by laws, and experts in various fields. Content analysis was employed. The study emphasizes the crucial roles of the government and Parliament in RIA development. Delegating RIA responsibilities to independent organizations aims to reduce legislation volume and improve its quality, contributing to a “good parliament” and an “effective government” following democratic principles and the Thai Constitution of 2017. The research underscores Parliament’s crucial role in standardizing law impact assessment. This promises better legislation quality, transparency, and accountability, aligning with global standards and encouraging public participation for improved governance and societal well-being.

Regulation of Ankyrin-G on Nav1.5 Channel in Hypoxic HL-1 Cardiac Muscle Cells

Regulation of Ankyrin-G on Nav1.5 Channel in Hypoxic HL-1 Cardiac Muscle Cells

Navigating Policy with the Logic of Regulatory Impact Assessment

Navigating Policy with the Logic of Regulatory Impact Assessment