Animal Welfare Impact Research Articles

A Review of the Effects of Pain and Analgesia on Immune System Function and Inflammation: Relevance for Preclinical Studies.

One of the most significant challenges facing investigators, laboratory animal veterinarians, and IACUCs, is how to balance appropriate analgesic use, animal welfare, and analgesic impact on experimental results. This is particularly true for in vivo studies on immune system function and inflammatory disease. Often times the effects of analgesic drugs on a particular immune function or model are incomplete or don't exist. Further complicating the picture is evidence of the very tight integration and bidirectional functionality between the immune system and branches of the nervous system involved in nociception and pain. These relationships have advanced the concept of understanding pain as a protective neuroimmune function and recognizing pathologic pain as a neuroimmune disease. This review strives to summarize extant literature on the effects of pain and analgesia on immune system function and inflammation in the context of preclinical in vivo studies. The authors hope this work will help to guide selection of analgesics for preclinical studies of inflammatory disease and immune system function.

Fish welfare: an NGO’s point of view

Fish are the most exploited, forgotten and misunderstood animals on the planet. They also are extraordinary creatures: complex, intelligent, sensitive, curious, and some of them have amazing abilities. For instance; some use tools, have a good memory, or collaborate to hunt. And most importantly, they are sentient, and they feel pain. Aquaculture has become the main supplier of fish worldwide, accounting for just over half of the fish eaten by humans due to static global wild-capture stocks, which have been overfished for decades. Also, global consumption of fish has doubled since the early 1970s and will continue to grow with population growth in the developing world. However, the aquaculture industry has developed without proper consideration of the needs of the fish species farmed, and the welfare consequences for those animals. Moreover, the rapid growth of aquaculture has raises major sustainability concerns due to its continued reliance on wild-caught fish. Annually, 0.5-1.0 trillion fish are caught to be reduced to ingredients to feed farmed animals, mainly fish. When considering the negative environmental consequences of using wild-caught fish as feed, we must not overlook the huge animal welfare impact that represents for the huge number of animals involved. Compassion in World Farming is working to raise awareness about fish sentience and the welfare problems that aquaculture industry represents for fish welfare. The way that fish are treated is important and we must do it better.

Brexit and Animal Welfare Impact Assessment: Analysis of the Opportunities Brexit Presents for Animal Protection in the UK, EU, and Internationally.

Simple SummaryThe British people voted in 2016 to leave the European Union (EU). The UK has a unique history as a leader in animal protection policy. It has a relatively large economy and significant political power on a global basis. Brexit presents both threats and opportunities to animal protection in the United Kingdom (UK), EU, and internationally. This paper assesses the opportunities Brexit presents for animal protection in terms of five criteria. These are first, the political situation; second, regulatory changes; third, economic and trade factors; fourth, institutional considerations; and fifth, EU and international impacts. Brexit provides the opportunity to reform UK farming to promote high animal welfare outside of the EU Common Agricultural Policy (CAP). Brexit means the UK can ban live animal exports and the import and sale of fur products and foie gras outside of the EU. Leaving the EU permits the UK to have stricter requirements for the Pet Travel Scheme (PETS) to control puppy smuggling. Brexit provides an opportunity for the UK Government to reform policy-making for sentient animals. New sentience legislation could establish a fully independent UK Animal Welfare Advisory body and mandate Government to use animal welfare impact assessments on all policy that affects sentient species. Despite such opportunities, the UK Government appears uncommitted to major reforms. The drafting of the Agriculture Bill does not suggest a progressive animal welfare agenda. For live exports, the Government will consult on how to improve welfare, rather than outright prohibition. Similarly, rather than ban the import and sale of fur, the Government will use its influence to improve the welfare of fur-farmed animals outside the UK. Brexit provides some opportunities for animal protection. Pre-Brexit, the Government has not demonstrated the political will and commitment to realise these opportunities.The British people voted in a 2016 referendum to leave the European Union (EU). Brexit presents threats and opportunities to animal protection in the United Kingdom (UK), the EU, and internationally. This paper discusses opportunities for animal protection in terms of five criteria. These are first, political context; second, regulatory changes; third, economic and trade factors; fourth, institutional- and capacity-related factors; and fifth, EU and international considerations. Brexit permits reform of UK agricultural policy outside of the Common Agricultural Policy (CAP) to reward high welfare as a public good. The Agriculture Bill, however, does not suggest a radical reform agenda for animal welfare. Brexit permits a ban on live exports, but the UK Government is consulting on improving welfare, not prohibition. Brexit provides an opportunity to ban the import and sale of fur, but the UK Government has signalled it will work to improve welfare in fur farming. Brexit permits the UK to prohibit the import and sale of foie gras, but the Government has stated a ban may be challenged at the World Trade Organisation (WTO). Brexit allows more stringent Pet Travel Scheme (PETS) requirements to reduce puppy smuggling. Lucy’s Law and stricter enforcement will also mitigate the problem. New sentience legislation provides the opportunity for a fully independent and properly constituted UK Animal Welfare Advisory body conducting animal welfare impact assessments and ethical appraisal. The Government has proposed sentience legislation but there is a major risk it will not be in place before the UK leaves the EU. The Government has expanded the remit of the Farm Animal Welfare Committee, which is not fully independent and is dominated by veterinary members and agricultural interests. Brexit provides some opportunities for animal protection with radical reform of agricultural policy, prohibition of live exports, and banning the import and sale of fur and foie gras. Pre-Brexit, the Government has not demonstrated the political will and commitment to realise these opportunities.

Animal experimentation: implementation and application of the 3Rs.

Despite the development of powerful molecular biological techniques and technologies, studies involving research animals remain a key component of discovery biology, and in the discovery and development of new medicines. In 1959, The Principles of Humane Experimental Technique, the 3Rs (Replacement, Reduction and Refinement) were developed to provide a framework to ensure animal research was undertaken as humanely as possible. Sixty years since their inception, the extent to which the 3Rs have been adopted and implemented by the global scientific and medical research communities has unfortunately been slow and patchy. However, this situation is changing rapidly as awareness increases, not only of the 3Rs themselves, but of the impact of animal welfare on the reproducibility, reliability and translatability of data from animal studies.

Influence of Pain and Analgesia on Orthopedic and Wound-healing Models in Rats and Mice.

The surgical stress response and resulting physiologic changes can lead to postoperative complications and negatively impact animal welfare. Although appropriate pain management is crucial to reduce the pain and stress response to surgery, analgesic choice can significantly affect bone and wound healing. This review aims to summarize data from rat and mouse studies and to provide recommendations for integrating analgesia into orthopedic and wound healing models in these species. Data from other species, such as humans, rabbits and other rodents, is included, where available. From these data, we conclude that for orthopedic surgical models, opioids, local anesthetics and dissociative agents have minimal impact on fracture healing; cyclooxygenase 2 (COX2) selective nonsteroidal antiinflammatory drugs (NSAID) may be used in the shortterm; and steroids should be avoided. For wound healing models, short-term systemic or topical opioids have negligible impact on wound healing; NSAID or local anesthetics may be used short-term; and systemic steroids should be avoided. Alternative analgesics such as tramadol, gabapentin, ketamine, and acetaminophen warrant consideration and further evaluation for both orthopedic and wound healing models. In all cases, researchers and veterinarians should work together to determine the appropriate analgesic plan to minimize pain, as well as to minimize unwanted effects on the orthopedic and wound healing models themselves.

Hashtag hijacking and crowdsourcing transparency: social media affordances and the governance of farm animal protection

The post-war Western world has seen a gradual shift from government to governance, a process that also concerned the issues related to agro-food sustainability, such as food quality, environmental impact, social justice, and farm animal welfare. Scholars believe that social media are a new site that reconfigures relations between various actors involved in the governance of these problems. However, empirical research on this matter remains scarce. This paper fills this gap by examining the case of Februdairy, a Twitter hashtag campaign to promote the British dairy industry, hijacked by animal protection activists. For this case, I employ the relational perspective on technology affordances—as operationalised by Faraj and Azad (in: Leonard et al. (eds), Materiality and organizing. Social interaction in a technological world, Oxford University Press, Oxford, 2012)—to highlight two distinct strategic modes of embracement of social media functionalities by the opposing groups: hashtag hijacking and crowdsourcing transparency. The analysis reveals also that a pre-existing social structure of the agro-food system conditions reconfiguration of social relations by technology in a way that actually strengthens the tendency to govern the issue of farm animal protection with market mechanisms.

Emerging threat ofEimeriaoperational taxonomic units (OTUs) on poultry production

Coccidiosis is an antagonistic poultry disease which negatively impacts animal welfare and productivity. The disease is caused by an obligate, intracellular protozoon known as Eimeria. Several Eimeria species known to infect chickens have been well documented. However, recent studies have elucidated the emergence of three novel genetic variants or operational taxonomic units (OTUs). The discovery of OTUx, OTUy and OTUz complicates the identification and diagnosis of coccidiosis. OTUs are clusters of unknown or uncultivated organisms that are grouped according to a similarity in DNA sequence to a set of specific gene markers. OTUs have been reported in the Earth's Southern Hemisphere, including Australia, Venezuela, India, Zambia, Uganda, Tanzania, China and Ghana. Elucidating their impact on the poultry industry is fundamental in preventing anticoccidial resistance and to access the potential of OTUs as vaccine candidates to provide cross-protection against similar Eimeria species. The identification of OTUs further decreases the risk of false negative coccidial diagnosis. Therefore, this article reviews the importance and risk imposed by OTUs, coupled with their prevalence and geographical distribution in chickens globally.

Multiple resistance to macrocyclic lactones in the sheep scab mite Psoroptes ovis

The astigmatid mite Psoroptes ovis (Acari: Proroptidae) causes the highly contagious and debilitating ovine disease, sheep scab. This ectoparasitic infection has a high economic and animal welfare impact on British sheep farming. Following recent work demonstrating resistance of Psoroptes mites to moxidectin, a widely used macrocyclic lactone (ML) treatment for scab, the current study compared the toxicity of three of the commonly administered macrocylic lactone therapeutic treatments (moxidectin, ivermectin and doramectin) to P. ovis from outbreak populations that had appeared unresponsive to treatment. These outbreak populations were from Wales and south west England. The data presented demonstrate that there is resistance to all three available ML compounds in populations of Psoroptes mites. However, considerable variation in response suggested that resistance alone was not responsible for the reported lack of efficacy in all of the submitted cases; lack of response in others may be associated with inappropriate treatment application or management. These data highlight the importance of the appropriate use of these compounds to manage national scab incidence at levels that are consistent with acceptable animal welfare standards, while attempting to reduce the development and spread of resistance.

THE IMPACT OF FOOD ANIMAL WELFARE ON HUMAN WELLBEING: SCIENTIFIC AND ISLAMIC PERSPECTIVES

Animal warfare includes consideration for all aspects of animal welfare and meeting their physical and mental needs. It generally concerns at keeping, handling, providing nutrition and disease prevention of animals especially in three important stages which are production, transportation, and slaughtering process for food animals. From the holistic medicine point of view, human wellbeing covers all aspects of physical, social, psychological and spiritual. About 80 percent of the human population of the World relies on animals for their food in which the food animals serve as the main source for most of the protein, fats, iron, and some vitamins. World Health Organization (WHO) reported that chronic diseases such as obesity, diabetes, cardiovascular and dental diseases, cancer and osteoporosis are related to human diet and nutrition. The welfare of food animals should be considered not only for the sake of animals but also for human wellbeing because the cruelty to animals is linked with animal stress which in turn have negative effects on human wellbeing. This paper uses the literature search and analytical method. It aims to explore the impact of food animal welfare to human wellbeing with the research scope on the Islamic perspectives as well as the scientific findings in the related area. Islam provides fundamental ethical guidelines in dealing with animals and highlights the importance of halalan toyyiban to achieve meat quality for human wellbeing. The scientific findings supported that mishandling of animals while keeping or slaughtering lead them to face physical and psychological stress which in turn affect the quality of the meat and chances of getting foodborne diseases. Both scientific and Islamic perspectives pointed out the requirement of animal welfare for human well-being. Thus, the welfare of food-producing animals such as ruminants and poultry should not be neglected since they have positive and negative consequences on human wellbeing. The food animal welfare should be implemented and integrated the existing and established guidelines of the meat industry to achieve the optimal value of human wellbeing.

The Impact of Animal Welfare and Environmental Information on the Choice of Organic Fish: An Empirical Investigation of German Trout Consumers

This article examines the effect of information on consumer preferences for farmed fish in the context of EU organic aquaculture production principles. A choice experiment was used to examine German consumers’ preferences for farmed rainbow trout. Respondents were split into separate groups, each receiving different levels of information about organic aquaculture production. The results show that most consumers have positive preferences for organic labeled fish, translating into an average additional willingness to pay €1.2/kg relative to nonorganic farmed trout. Informing consumers specifically about animal welfare consequences associated with the organic label significantly increases the likelihood of choosing the labeled product and increases the marginal willingness to pay €2.4/kg for organic trout. No effect was found when providing additional information about environmental consequences. Hence, focusing on animal welfare when promoting organic aquaculture production is likely to resonate with consumers, thereby potentially increasing market shares and producer revenues.

Prospects for predictive modeling of transition cow diseases.

Transition cow diseases can negatively impact animal welfare and reduce dairy herd profitability. Transition cow disease incidence has remained relatively stable over time despite monitoring and management efforts aimed to reduce the risk of developing diseases. Dairy cattle disease risk is monitored by assessing multiple factors, including certain biomarker test results, health records, feed intake, body condition score, and milk production. However, these factors, which are used to make herd management decisions, are often reviewed separately without considering the correlation between them. In addition, the biomarkers that are currently used for monitoring may not be representative of the complex physiological changes that occur during the transition period. Predictive modeling, which uses data to predict future or current outcomes, is a method that can be used to combine the most predictive variables and their interactions efficiently. The use of an effective predictive model with relevant predictors for transition cow diseases will result in better targeted interventions, and therefore lower disease incidence. This review will discuss predictive modeling methods and candidate variables in the context of transition cow diseases. The next step is to investigate novel biomarkers and statistical methods that are best suited for the prediction of transition cow diseases.

Infectious Disease Outbreak Associated With Supplementary Feeding of Semi-domesticated Reindeer.

Supplementary winter feeding of semi-domesticated reindeer (Rangifer tarandus tarandus) has become more common in Sweden and Norway due to reindeer pasture fragmentation and climatic conditions. With increased corralling and feeding, often associated with animal stress, increased animal-to-animal contact, and poor hygienic conditions, an altered range of health challenges and diseases may emerge. An outbreak of three different infectious diseases appeared simultaneously in a reindeer herd in Norrbotten County, Sweden. The animals were corralled and fed silage. Several animals in poor body condition stopped eating, with drool and discoloration of the hair coat around the mouth. There were large, black, necrotic lesions on the tongue and gingiva, with holes perforating the chin, indicative of oral necrobacillosis and Fusobacterium spp. infection. Simultaneously, animals were seen with proliferative lesions in the oral mucosa and on the lips, characteristic of contagious ecthyma and Orf virus infection. Furthermore, three animals had keratoconjunctivitis suggesting exposure to cervid herpesvirus 2 (CvHV2) and possibly secondary bacterial infections. DNA specific for Fusobacterium necrophorum and ORFV was detected in relevant tissue samples. Antibodies against CvHV2 were detected in 10 of 13 diseased and in four of 11 apparently healthy reindeer. Nine animals were found dead or were euthanized during the outbreak. Health risk factors associated with feeding and corralling may severely impact animal welfare and the herder's economy, and may represent an underestimated cost when replacing natural grazing with feeding.

Brexit and Animal Welfare Impact Assessment: Analysis of the Threats Brexit Poses to Animal Protection in the UK, EU and Internationally.

Simple SummaryThe British people voted in a 2016 referendum to leave the European Union and the United Kingdom (UK) is set to leave the EU in 2019. Brexit is a major political change and it presents both threats and opportunities for animal protection. This paper assesses the threats that Brexit poses to animal protection in terms of five criteria. These are first, the political situation; second, regulatory changes; third, economic and trade factors; fourth, institutional considerations; and fifth, EU and international impacts. The EU has the most progressive animal welfare laws in the world. The UK Conservative Government, which is delivering Brexit, has a mixed record on animal protection. Brexit is forecast to have a negative impact on the UK economy, which is likely to negatively affect animal welfare. A major threat of Brexit is the import of meat and dairy products to the UK raised in lower welfare standards from nations such as the United States (US). The development of Brexit policy suggests there is a significant risk that this threat will materialise. Furthermore, Brexit will result in a reduced political lobby within the EU for progressive animal protection reform. Despite the UK being a progressive animal protection nation, she will have less power to exert this influence to improve animal welfare outside of the EU. Brexit poses substantial risks to weaken animal protection in the UK, EU and internationally. Further research is needed to assess the opportunities presented by Brexit to judge whether Brexit will be overall positive or negative for animal protection.The British people voted in a 2016 referendum to leave the European Union (EU). Brexit presents both threats and opportunities to animal protection in the United Kingdom (UK), EU and internationally. This paper discusses threats to animal protection in terms of five criteria. These are first, political context; second, regulatory changes; third, economic and trade factors; fourth, institutional and capacity-related factors; and fifth, EU and international considerations. The EU has the most progressive animal welfare laws in the world. The Conservative Government delivering Brexit has a mixed record on animal protection. Major time and resource constraints inherent in Brexit risk negatively impacting animal protection. Brexit is projected to have a negative economic impact, which is generally associated with lower animal welfare standards. The development of Brexit policy suggests there to be a substantial risk that the major threat of importing lower welfare products to the UK will materialise. Brexit will reduce the political influence of the progressive animal protection lobby in the EU. Post-Brexit, the politically and economically weakened EU and UK risks a detrimental impact on animal protection on an international scale. Brexit poses substantial threats to animal protection, with a high risk that many threats will materialise. Further research is needed to assess the opportunities presented by Brexit to judge whether Brexit will be overall positive or negative for animal protection.

Attitudes toward Animals and Their Welfare among Italian Veterinary Students

As members of the public and the veterinary profession are increasingly concerned about animal welfare, there has been an increased scholarly interest in the attitudes of veterinarians and students toward animals, as these may impact human behavior, which ultimately impacts animal welfare. Here we investigated Italian veterinary students’ demographic data and perceptions about nonhuman animal welfare issues that might be predictive of their attitudes. A survey eliciting information about demographics, knowledge, experience, and perceptions regarding different categories of animals, and including the Animal Attitude Scale (AAS), was administered to undergraduate veterinary medicine students in three Italian universities. Data were analyzed using nonparametric tests, and a value of p < 0.05 was considered statistically significant. In total, 876 students completed the questionnaire, with females (75.1%) making up a majority of students in all years of the course. Although veterinary students showed pro-animal welfare attitudes (mean score = 64.20 ± 0.24 out of 100), the findings suggested that year of study, gender, and geographical location had a significant impact (p < 0.05). In this study, we found a set of factors that, either individually or combined, help predict a student’s attitude toward animal welfare issues, which will be useful in improving the curriculum strategy in veterinary education in Italy.

The Effect of Virtual Fencing Stimuli on Stress Responses and Behavior in Sheep.

Simple SummaryVirtual fencing is a new technology that uses audio signals and electrical stimuli to spatially control animals without the need for fixed fencing. It involves avoidance learning whereby the animals learn to respond to an audio cue (conditioning stimulus) to avoid receiving an aversive electrical stimulus (unconditioned stimulus). The audio cue is used to warn the animal that it is approaching the boundary and should be benign and not perceived as aversive to the animal. While a positive punishment stimulus is necessary for learning, it should not be so aversive to the animal that it impinges on its welfare. This study aimed to determine how the stimuli used in virtual fencing are perceived by the animal when they are first encountered. The audio and electrical stimuli were compared to other commonly encountered stimuli in normal sheep production systems, including a barking dog and a restraint procedure. The physiological and behavioral responses of sheep indicated that sheep were no more adversely impacted by virtual fencing stimuli than they were by other commonly encountered stimuli. The least to most aversive treatments were: Control < Beep < Barking Dog < Electrical stimulus < Restraint.To understand the animal welfare impact of virtual fencing stimuli (audio cue ‘beep’ and electrical stimulus) on naïve sheep, it is necessary to assess stress responses during the animal’s first encounters with these stimuli. Eighty Merino ewes were exposed to one of the following treatments (n = 16 animals per treatment): Control (no stimuli), beep, dog bark, manual restraint, and electrical stimulus. Collars were used to apply the audio and electrical stimuli. The restraint treatment showed an elevated cortisol response compared with the control (p < 0.05), but there were no differences between the other treatments and the control. There were no differences between treatments in vaginal temperature (p > 0.05). For behaviors, the sheep receiving the bark and beep treatments were more vigilant compared to the control (p < 0.05), there were more aversive responses observed in the electrical stimulus treatment compared to the control. Together, the responses showed that the beep stimuli were largely benign, the bark stimuli was minimally aversive, the electrical stimuli was acutely aversive, and the restraint was moderately aversive. These data suggest that, for sheep, their first exposure to the virtual fencing stimuli should be perceived as less aversive than a commonly used restraint procedure.

A viral video and pet lemurs on Twitter.

Content shared on social media platforms can impact public perceptions of wildlife. These perceptions, which are in part shaped by context (e.g. non-naturalistic setting, presence of a human), can influence people’s desires to interact with or acquire wild animals as pets. However, few studies have examined whether this holds true for wild animals featured in viral videos. This study reports on opportunistic data collected on Twitter before, during, and after a video that featured a habituated ring-tailed lemur (Lemur catta), called “Sefo”, in southern Madagascar went ‘viral’ (i.e. circulated rapidly on the internet). Our dataset of 13,953 tweets (from an 18.5-week time period in early 2016) referencing lemurs was collected using targeted keywords on the Twitonomy Service. We identified 613 individual tweets about people wanting a lemur as a pet. In addition, 744 tweets that were captured in our dataset linked to the Sefo viral video. We found that as the number of tweets about the viral video increased, so did the number of tweets where an individual wanted to have a lemur as a pet. Most tweets (91%) did not make reference to a specific species of lemur, but when they did, they often (82%) referenced ring-tailed lemurs (L. catta), ruffed lemurs (Varecia spp.), and mouse lemurs (Microcebus spp.). This study serves as a case study to consider how viral content can impact how wild animals are perceived. We close by noting that social media sites like Twitter, which are increasingly providing their users with news and information, should carefully consider how information about wild animals is shared on their platforms, as it may impact animal welfare.

The challenge of incorporating animal welfare in a social life cycle assessment model of European chicken production

PurposeThere is increasing public concern over standards of farm animal welfare, yet the majority of sustainability studies of livestock have thus far focused only on environmental performance and profitability. Where social analysis has been carried out, there has yet to be a consistent methodology developed that incorporates animal welfare into social life cycle assessment (S-LCA). A framework was developed to assess animal welfare, using conventional broiler chicken meat production in Europe as a case in point.MethodsData were collected on stocking density, mortality, and carcass condemnation rate from conventional chicken meat production systems in Europe. The quantitative risk of each welfare indicator was characterised in accordance with the Social Hotspots Database methodology based on best to worst farm performances, i.e. quartiles of the data collected for each indicator. The overall animal welfare impact was assessed using a weighted sum methodology, which accounted for the level of risk animals were exposed to for each indicator and the animal lifespan. From this, a Social Hotspot Index (SHI) could be calculated for the animal welfare impact associated with the functional unit, which was 1 kg of chicken meat production. The animal welfare impact of four European countries was then compared.Results and discussionThe countries assessed displayed a range of values for overall animal welfare impact; the country with the best animal welfare had a SHI for animal welfare impact of 0.14, whilst the worst had a SHI for animal welfare impact of 0.72. Farms that kept more birds per building had an increased overall animal welfare impact. Animal welfare, determined by negative welfare indicators, was worse in more recently established farm buildings due to increased flock size.ConclusionsA methodology that incorporates animal welfare indicators into S-LCA was developed that is both scalable and related to welfare assessment frameworks. Although only some specific negative welfare indicators were considered here, the methodology could easily accommodate additional negative indicators and even positive welfare indicators as advancements are made in the understanding of animal welfare. Hence, this study provides a springboard for further development of S-LCA, animal welfare assessment and, ultimately, improved animal welfare in livestock systems.

Brexit and Animal Protection: Legal and Political Context and a Framework to Assess Impacts on Animal Welfare.

Simple SummaryThe British public voted to leave the European Union (EU) in a 2016 referendum and the United Kingdom (UK) is set to leave in March 2019. The UK has been part of the EU or the European Communities (EC) before it for around 50 years. Britain has had a major impact on EU animal protection laws and the UK as a member state has been substantially influenced by EU law. Brexit represents a major political change that will affect animal protection in the UK, the EU and internationally. Given the far greater numbers of animals used in agriculture, the impact of the UK’s departure from the EU on farm animals will determine whether Brexit is overall good or bad for animal protection. A major threat that Brexit poses is importing lower welfare meat and dairy products to the UK. A major opportunity post-Brexit is reform of agricultural policy to reward high animal welfare outside of the EU Common Agricultural Policy (CAP). A soft Brexit, where the UK remains aligned to the EU in policy and trade, reduces the risks Brexit poses to animal protection. A hard Brexit means major threats to animal welfare are more likely to materialise. Further research is required to investigate whether the various threats and opportunities are likely to materialise and whether Brexit will be, all things considered, good or bad for animal protection.The British people voted to leave the European Union (EU) in a 2016 referendum. The United Kingdom (UK) has been a member of the EU since the Maastricht Treaty was signed in 1993 and before that a member of the European Communities (EC) since 1973. EU animal health and welfare regulations and directives have had a major impact on UK animal protection policy. Similarly, the UK has had a substantial impact on EU animal protection. Brexit represents a substantial political upheaval for animal protection policy, with the potential to impact animal welfare in the UK, EU and internationally. Brexit’s impact on farmed animals will determine the overall impact of Brexit on animals. A major threat to animal welfare is from importing lower welfare products. A major opportunity is reform of UK agricultural policy to reward high welfare outside the Common Agricultural Policy (CAP). A soft Brexit, in which the UK remains in the single market and/or customs union, mitigates the threat of importing lower welfare products. A harder Brexit means threats to animal welfare are more likely to materialise. Whether threats and opportunities do materialise will depend on political considerations including decisions of key political actors. The Conservative Government delivering Brexit has a problematic relationship with animal protection. Furthermore, Brexit represents a shift to the political right, which is not associated with progressive animal protection. There is significant political support in the Conservative Party for a hard Brexit. Further research is required to investigate whether the various threats and opportunities are likely to materialise.

Standard Rodent Housing Conditions Produce Significant and Sustained Levels of Particulate Matter

While the mass of airborne particulate matter have established health guidelines for humans, similar recommendations are not available for laboratory rodent cages. Several studies have assessed the airborne contaminants produced by laboratory animals at the macroenvironment room level to reduce occupational health hazards for those working in animal facilities. These studies have led to established guidelines for protocols such as frequency of cage changes, animal room ventilation rates, and frequency of animal room air changes. However, microenvironmental cage measurements can differ greatly from the macroenvironmental room measurements. Cage-level particulate matter studies are needed to ensure that the laboratory rodent microenvironment is not having a detrimental effect on animal welfare and validity and relevance of scientific data. The objective of the current study was to assess PM levels produced by common rodent bedding types in bar lid and filter top covered rat cages. PM levels were assessed following artificial manipulation of the bedding (designed to simulate typical rodent activity) and during an hour of activity by two adult rats following a standard cage change using pine, aspen, paper, and corn cob bedding. Simulated activity and rat induced disruption of all types of bedding produced significant and substantial increases in PM (1000-6000 µg/m3 PM2.5), with elevated levels (&amp;gt;50ug/m3) sustained for longer durations in cages with filter tops (5-6 minutes) compared to cages with only bar lids (0-2 minutes). These results indicate that standard laboratory housing conditions produce differentially substantial levels of PM that are likely to impact animal welfare and/or experimental data in animal studies of PM exposure.

Animal- and management-based welfare indicators for a conventional broiler strain in 2 barn types (Louisiana barn and closed barn).

The aim of this observational study was to describe health- and management-related welfare indicators for a conventional broiler strain housed in 2 barn types (Louisiana barn and closed barn) on the same farm and to assess the impact of age and housing conditions on these indicators. Broilers were examined 4 times in each of 7 fattening periods. Their weight gain, gait score, and further animal-based indicators did not differ between the barn types. On average 46% showed no sign, 51% a minor but visible, and 3% a profound lameness at the end of the fattening period (fattening day 39). Soiling of the plumage, skin scratches, foot pad dermatitis, and hock burns worsened with increasing age. Soiling of the plumage was correlated negatively with litter depth (Pearson, r = -0.549, P = 0.042) and positively with litter quality (Pearson, r = 0.641, P = 0.013). Skin scratches occurred in 89% of the broilers (32% deep with penetration of dermis) on fattening day 39 and were correlated positively with cumulative mortality (Pearson, r = 0.615, P = 0.019), indicating a severe animal welfare impact. Foot pad dermatitis did not correlate with health- or management-related indicators, whereas hock burn correlated positively with the broilers' weight (Pearson, r = 0.853, P < 0.001) and with ammonia concentrations (Pearson, r = 0.577, P = 0.031). Management-related indicators (antibiotic treatments, dead on arrival) and cumulative mortality did not differ between the barn types. At the end of the fattening period, the litter quality was worse and concentrations of ammonia and peaks of high carbon dioxide concentrations were higher in the Louisiana than in the closed barn. The light intensity was on average 20 times higher in the Louisiana than in the closed barn without any negative impact. Summarizing, the barn type did not seem to influence the investigated welfare indicators, but Louisiana barns might need a more precise management to maintain the required ranges of noxious gases and litter quality.