Ability Of Imaging Research Articles

Dynamic nano-imaging via a microsphere compound lens integrated microfluidic device with a 10× objective lens.

Optical microscopic imaging techniques are essential in biology and chemistry fields to observe and extract dynamic information of micro/nano-scale samples in microfluidic devices. However, the current microfluidic optical imaging schemes encounter dilemmas in simultaneously possessing high spatial and temporal resolutions. Recently, microsphere nanoscope has emerged as a competitive nano-imaging tool due to its merits like high spatial resolution, real-time imaging abilities, and cost-effectiveness, which make it a potential solution to address the aforementioned challenges. Here, a microsphere compound lens (MCL) integrated microfluidic imaging device is proposed for real-time super-resolution imaging. The MCL consists of two vertically stacked microspheres, which can resolve nano-objects with size beyond the optical diffraction limit and generate an image of the object with a magnification up to 10×. Exploiting the extraordinary nano-imaging and magnification ability of the MCL, optically transparent 100 nm polystyrene particles in flowing fluid can be discerned in real time by the microfluidic device under a 10× objective lens. Contrary to this, the single microsphere and the conventional optical microscope are incompetent in this case regardless of the magnification of objective lenses used, which demonstrates the superiority of the MCL imaging scheme. Besides, applications of the microfluidic device in nanoparticle tracing and live-cell monitoring are also experimentally demonstrated. The MCL integrated microfluidic imaging device can thus be a competent technique for diverse biology and chemistry applications.

Multiparametric Magnetic Resonance Imaging Correlates of Isocitrate Dehydrogenase Mutation in WHO high-Grade Astrocytomas.

Isocitrate dehydrogenase (IDH) mutation and O-6 methyl guanine methyl transferase (MGMT) methylation are surrogate biomarkers of improved survival in gliomas. This study aims at studying the ability of semantic magnetic resonance imaging (MRI) features to predict the IDH mutation status confirmed by the gold standard molecular tests. The MRI of 148 patients were reviewed for various imaging parameters based on the Visually AcceSAble Rembrandt Images (VASARI) study. Their IDH status was determined using immunohistochemistry (IHC). Fisher's exact or chi-square tests for univariate and logistic regression for multivariate analysis were used. Parameters such as mild and patchy enhancement, minimal edema, necrosis < 25%, presence of cysts, and less rCBV (relative cerebral blood volume) correlated with IDH mutation. The median age of IDH-mutant and IDH-wild patients were 34 years (IQR: 29-43) and 52 years (IQR: 45-59), respectively. Mild to moderate enhancement was observed in 15/19 IDH-mutant patients (79%), while 99/129 IDH-wildtype (77%) had severe enhancement (p-value <0.001). The volume of edema with respect to tumor volume distinguished IDH-mutants from wild phenotypes (peritumoral edema volume < tumor volume was associated with higher IDH-mutant phenotypes; p-value < 0.025). IDH-mutant patients had a median rCBV value of 1.8 (IQR: 1.4-2.0), while for IDH-wild phenotypes, it was 2.6 (IQR: 1.9-3.5) {p-value = 0.001}. On multivariate analysis, a cut-off of 25% necrosis was able to differentiate IDH-mutant from IDH-wildtype (p-value < 0.001), and a cut-off rCBV of 2.0 could differentiate IDH-mutant from IDH-wild phenotypes (p-value < 0.007). Semantic imaging features could reliably predict the IDH mutation status in high-grade gliomas. Presurgical prediction of IDH mutation status could help the treating oncologist to tailor the adjuvant therapy or use novel IDH inhibitors.

Dopamine and DNA functionalized manganese whitlockite nanocrystals for magnetic resonance imaging and chemo-photothermal therapy of tumors

Multifunctional inorganic nanomaterials have opened new avenues for cancer diagnosis and therapy. However, the difficulty to functionalize them has prevented their wider application, owing to the lack of active groups on their surfaces. Here we report a novel method to functionalize manganese whitlockite (Mn-WH) with polydopamine (PDA) and hairpin DNA (hpDNA) to improve the water stability and anti-cancer effects of Mn-WH nanoparticles (Mn-WH NPs). Compared to WH NPs, the Mn-WH@PDA-hpDNA NPs exhibit better water dispersibility, high drug loading capacity, excellent photothermal performance, stable MRI imaging ability, and outstanding chemo-photothermal synergistic therapeutic potential against tumors. After intratumoral injection in nude mice, the Mn-WH@PDA-hpDNA-DOX NPs promote complete tumor ablation upon exposure to 808 laser-irradiation. The nanoparticles showed no major side effects or toxicity. Thus, these results indicate that the Mn-WH@PDA-hpDNA-DOX NPs have excellent potential as anti-cancer agents, along with excellent magnetic resonance imaging (MRI) capabilities and the reported functionalization approach provides a novel and effective strategy for the surface functionalization of inorganic nanomaterials.

A lipid activatable fluorescence probe for atherosclerosis imaging

Lipid has been considered as a promising target for atherosclerosis diagnosis. However, there is still no available lipid imaging technology in clinic. Herein, we have prepared a fluorescence probe TPN for lipid-specific imaging in atherosclerosis. TPN exhibited extremely weak emission in water, while its emission was significantly enhanced in lipid environment at 666 nm. Meanwhile, TPN has showed low cytotoxicity and great intracellular lipid-specific fluorescence imaging ability with high signal-to-noise ratio. Importantly, TPN could specifically stain the lipid in atherosclerotic plaque, which would be a potential candidate for the diagnosis of atherosclerosis.

An Oligopeptide-Protected Ultrasmall Gold Nanocluster with Peroxidase-Mimicking and Cellular-Imaging Capacities.

Recent decades have witnessed the rapid progress of nanozymes and their high promising applications in catalysis and bioclinics. However, the comprehensive synthetic procedures and harsh synthetic conditions represent significant challenges for nanozymes. In this study, monodisperse, ultrasmall gold clusters with peroxidase-like activity were prepared via a simple and robust one-pot method. The reaction of clusters with H2O2 and 3,3',5,5'-tetramethylbenzidine (TMB) followed the Michaelis-Menton kinetics. In addition, in vitro experiments showed that the prepared clusters had good biocompatibility and cell imaging ability, indicating their future potential as multi-functional materials.

Accuracy of combined physical examination, transvaginal ultrasonography, and magnetic resonance imaging to diagnose deep endometriosis

To assess the ability of Physical Examination (PE), Transvaginal Ultrasonography (TVUS), and Magnetic Resonance Imaging (MRI) alone and combined to diagnose Deep Infiltrating Endometriosis (DIE). We retrospectively queried our pelvic MRI database to identify women who underwent PE, TVUS, and pelvic MRI for DIE up to 12 months prior to surgery between January 1, 2016 and August 31, 2020. The presence of uterosacral ligaments (USL), vaginal, rectosigmoid (RS), parametrial or sacrorectogenital septum (lateral) DIE shown by PE, TVUS and MRI were correlated with surgical and histological findings. We included 178 patients. The sensitivity, specificity, positive and negative predictive values, and accuracy of each technique separately and combined were assessed for each location. When the three techniques were combined, two models were tested: A) all three techniques positive and concordant, and B) at least two techniques positive and concordant. The prevalence of USL, vaginal, RS, and lateral DIE were 94.4%, 20.2%, 34.3% and 32.6%, respectively. MRI was more sensitive than PE, TVUS or any combination to detect DIE. MRI and model B were the most accurate for detecting USL and RS locations with an accuracy of 90.4% and 82.6%, a sensitivity of 91.1% and 50%, and a specificity of 77.8% and 90.9%, respectively. Model B was the most accurate for the vaginal location with an accuracy of 82.6%, a sensitivity of 50%, and a specificity of 90.9%. Finally, MRI was more accurate than any combination for identifying a lateral location with an accuracy of 75.1%, a sensitivity of 36%, and a specificity of 93.8%. A combination of PE, TVUS and MRI more accurate than each technique separately to diagnose DIE due to the equally high sensitivity of each, and the high specificity of PE and TVUS.

Red light-emitting carbon dots for reduced phototoxicity and photothermal/photodynamic-enhanced synergistic tumor therapy

Photodynamic therapy (PDT) has received extensive attention due to its non-invasive, photo-stimuli-responsive advantages. However, the poor dispersibility of most photosensitizers in water and non-negligible phototoxicity limit their further clinical application. To surmount this dilemma, red-emitting carbon dots (RCDs) with good photothermal conversion ability were synthesized, and photosensitizers Chlorin-e6 (Ce6) were linked onto RCDs to construct the nano-platform RCDs-Ce6 through amide reaction. In this system, RCDs can increase the dispersion of Ce6 to enhance PDT and absorb partitions of daylight to reduce phototoxicity. At the same time, RCDs-Ce6 can achieve simultaneous excitation of photothermal therapy (PTT) and PDT under a single laser, which can overcome the drawbacks of monotherapy while simplifying the treatment procedure. Besides, the excellent fluorescence imaging capability of RCDs-Ce6 could achieve imaging-guided tumor treatment. Spontaneously, both the cytological and 3D multicellular spheroids (3D MCS) levels demonstrated that RCDs-Ce6 had a stronger synergic therapeutic effect with a combination index (CI) of 0.278 and lower phototoxicity. Meanwhile, in vivo experiments showed that RCDs-Ce6 (PTT and PDT) had a more substantial tumor inhibition effect and fluorescence imaging ability with more tumor distribution. This study widened the way for further applications of photodynamic-enhanced therapy with reduced phototoxicity in photosensitizer delivery based on RCDs.

Multispectral UAV-Based Monitoring of Leek Dry-Biomass and Nitrogen Uptake across Multiple Sites and Growing Seasons

Leek farmers tend to apply too much nitrogen fertilizer as its cost is relatively low compared to the gross value of leek. Recently, several studies have shown that proximal sensing technologies could accurately monitor the crop nitrogen content and biomass. However, their implementation is impeded by practical limitations and the limited area they can cover. UAV-based monitoring might alleviate these issues. Studies on UAV-based vegetable crop monitoring are still limited. Because of the economic importance and environmental impact of leeks in Flanders, this study aimed to investigate the ability of UAV-based multispectral imaging to accurately monitor leek nitrogen uptake and dry biomass across multiple fields and seasons. Different modelling approaches were tested using twelve spectral VIs and the interquartile range of each of these VIs within the experimental plots as predictors. In a leave-one-flight out cross-validation (LOF-CV), leek dry biomass (DBM) was most accurately predicted using a lasso regression model (RMSEct = 6.60 g plant−1, R2= 0.90). Leek N-uptake was predicted most accurately by a simple linear regression model based on the red wide dynamic range (RWDRVI) (RMSEct = 0.22 gN plant−1, R2 = 0.85). The results showed that randomized Kfold-CV is an undesirable approach. It resulted in more consistent and lower RMSE values during model training and selection, but worse performance on new data. This would be due to information leakage of flight-specific conditions in the validation data split. However, the model predictions were less accurate for data acquired in a different growing season (DBM: RMSEP = 8.50 g plant−1, R2 = 0.77; N-uptake: RMSEP = 0.27 gN plant−1, R2 = 0.68). Recalibration might solve this issue, but additional research is required to cope with this effect during image acquisition and processing. Further improvement of the model robustness could be obtained through the inclusion of phenological parameters such as crop height.

Arrangement of Indocyanine Green in a 1.5-Nanometer Channel to Achieve High-Efficiency Imaging of the Intestinal Lymphatic System.

The complications of inflammatory bowel diseases (IBDs) seriously endanger people’s health, such as bleeding, polyp hyperplasia, and even cancer. Although the precise pathophysiology of IBD is unknown, alterations in the intestinal lymphatic network, such as lymphangiogenesis and lymphatic vessel dysfunction, are well-established features. Therefore, the development of a reliable technology is urgently required, with a stereoscopic, deep, and high-resolution technology for IBD lymphatic targeting imaging in clinical practice. However, indocyanine green, the only clinically approved imaging agent by the Food and Drug Administration, can easily cause self-aggregation or be interfered with by microenvironments, causing fluorescence quenching, which seriously affects the imaging and detective capabilities. Herein, indocyanine green molecules are arranged in a 1.5-nanometer one-dimensional channel (TpPa-1@ICG). Based on this specified structure, the fluorescence enhancement effect is observed in the TpPa-1@ICG resultant, and the fluorescence intensity is enhanced by 27%. In addition, the ICG-incorporated porous solid reveals outstanding solvent (dichloromethane, tetrahydrofuran, etc.) and thermal (>300 °C) stability. After modifying the target molecules, TpPa-1@ICG showed excellent imaging ability for intestinal lymphatic vessels, providing a new imaging tool for IBDs research.

Flexible Method for Generating Arbitrary Vector Beams Based on Modified Off-Axis Interference-Type Hologram Encoding

A novel experimental setup for the generation of arbitrary vector beams is proposed. The system major includes two reflective liquid crystal spatial light modulators (RLC-SLM) and a polarizing beam splitting prism. Moreover, this method is not limited by the wavelength of light wave and the pixel size of SLM. Theoretical analysis shows that when Gaussian beam or a plane beam is illuminated on a computer-generated hologram (CGH) specially designed in this work, the complex amplitudes of the vector field’s two orthogonal polarization components may be changed by modifying the encoding parameters, resulting in a vector beam with arbitrary complex amplitude and polarization in the output field. The experimental results also show that the two independent coding channels of the device have good polarization-selective imaging ability, which greatly improves the flexibility of generating arbitrary vector beams.

Amide Proton Transfer-Weighted Imaging Detects Hippocampal Proteostasis Disturbance Induced by Sleep Deprivation at 7.0 T MRI.

Sleep deprivation leads to hippocampal injury. Proteostasis disturbance is an important mechanism linking sleep deprivation and hippocampal injury. However, identifying noninvasive imaging biomarkers for hippocampal proteostasis disturbance remains challenging. Amide proton transfer-weighted (APTw) imaging is a chemical exchange saturation transfer technique based on the amide protons in proteins and peptides. We aimed to explore the ability of APTw imaging in detecting sleep deprivation-induced hippocampal proteostasis disturbance and its biological significance, as well as its biological basis. In vitro, the feasibility of APTw imaging in detecting changes of the protein state was evaluated, demonstrating that APTw imaging can detect alterations in the protein concentration, conformation, and aggregation state. In vivo, the hippocampal APTw signal declined with increased sleep deprivation time and was significantly lower in sleep-deprived rats than that in normal rats. This signal was positively correlated with the number of surviving neurons counted in Nissl staining and negatively correlated with the expression of glucose-regulated protein 78 evaluated in immunohistochemistry. Differentially expressed proteins in proteostasis network pathways were identified in the hippocampi of normal rats and sleep-deprived rats via mass spectrometry proteomics analysis, providing the biological basis for the change of the hippocampal APTw signal in sleep-deprived rats. These findings demonstrate that APTw imaging can detect hippocampal proteostasis disturbance induced by sleep deprivation and reflect the extent of neuronal injury and endoplasmic reticulum stress.

Fluorescence-optical imaging as a promising easy-to-use imaging biomarker to increase early psoriatic arthritis detection in patients with psoriasis: a cross-sectional cohort study with follow-up

ObjectivesTo evaluate the ability of fluorescence-optical imaging (FOI) to detect preclinical musculoskeletal inflammatory signs in patients with skin psoriasis at risk of developing psoriatic arthritis (PsA).MethodsThis investigator-initiated prospective exploratory study...

Inclusion Body Myositis

This article highlights the clinical and diagnostic features of inclusion body myositis (IBM) and provides recent insights into the pathomechanisms and therapeutic strategies of the disease. IBM is an often-misdiagnosed myopathy subtype. Due to the insidious onset and slow progression of muscle weakness, it can often be dismissed as a sign of aging as it commonly presents in older adults. While challenging to recognize upon initial clinical evaluation, the recent recognition of specialized stains highlighting features seen on muscle pathology, the use of diagnostic tools such as the anti-cytosolic 5'-nucleotidase 1A antibody biomarker, and the ability of muscle imaging to detect patterns of preferential muscle involvement seen in IBM has allowed for earlier diagnosis of the disease than was previously possible. While the pathogenesis of IBM has historically been poorly understood, several ongoing studies point toward mechanisms of autophagy and highly differentiated cytotoxic T cells that are postulated to be pathogenic in IBM. Overall advancements in our understanding of IBM have resulted in improvements in the management of the disease and are the foundation of several strategies for current and upcoming novel therapeutic drug trials in IBM.

The Leaves Are Stirring (1948)

Abstract This paper presents the first English-language translation of “Les feuilles bougent” (“The Leaves Are Stirring”), writer-director Nicole Vedrès's most important essay on film aesthetics. Published in August 1948 in the illustrious revue, Les Temps Modernes, Vedrès's essay reflects on her then-recent experience of directing the celebrated archival documentary, Paris 1900 (1947). Using her experience of searching for and reediting footage of turn-of-the-century Paris as a springboard, Vedrès tackles a set of aesthetic problems that were being posed with renewed vigor among French film writers in the period after the war. These include questions of cinema's relationship to the real, the ability of images to capture historical change, and the relationship between cinematic montage and the act of writing.

Novel, Green, and Scalable Aqueous Synthesis of Yellow–Green Emitting Cs3Cu2Cl5 Scintillator and its Application in High‐Resolution TFT Panel for X‐Ray Imaging Detector

AbstractCopper‐based metal halide scintillators have received increasing attention due to their high light yield and nontoxicity. However, the synthetic methods are still not feasible for actual commercialization for the difficulty in scaling up and the absence of green chemical footprint. Herein, we report a new, simple, aqueous synthetic method for the mass production of highly purified and crystallized Cs3Cu2Cl5 powder by a room‐temperature reaction between a saturated aqueous solution of CsCl and a saturated hydrochloric acid solution of CuCl. The product exhibits a photoluminence peak centering at 527 nm (FWHM = 106.7 nm) and a quantum yield (PLQY) of 97%. A prototype X‐ray imaging detector is fabricated by coupling a large area scintillation screen prepared by blade coating of Cs3Cu2Cl5 powder and a commercial thin‐film transistor (TFT) panel (16 cm × 13 cm, 125 µm pixel size) with readout circuit. The device exhibits a high spatial resolution of 4 lp mm−1, exceeding the theoretical limit of the TFT panel, and an excellent dynamic imaging ability. In short, this work demonstrates an efficient novel synthetic method of Cs3Cu2Cl5 and its application as X‐ray imaging device, providing an applicable solution for its future commercialization.

Delineating the fluvial sedimentary reservoirs of Cretaceous sedimentation using seismic attributes and seismic‐petrophysical simulations, Southwest Pakistan

Channels filled with coarse‐grained sands are one of the hot issues in stratigraphic explorations. However, the sub‐surface uncertainty restricts the prediction of porous channel sandstones using the conventional seismic attributes, which is a concern for the demarcation of stratigraphic plays. The amplitude‐based frequency attributes and seismic‐petrophysical simulation tools were applied to characterize the fluvial channel system from the Indus Basin, Pakistan. The conventional amplitude of the Basal Sand Formation shows limited imaging ability to detect the dimensions of channel‐levee and point bars. The continuous wavelet transforms (CWT) tool distinguishes the whole channel‐levee reservoir system at tuning frequencies of 21, 25, 29, and 34 Hz. The amplitudes extracted along the pseudo‐porosity wells depict the low‐density and low‐velocity profiles within the gas‐bearing zones. The zone between 20% and 30% porosity displays low‐density and low‐velocity compartments within the point bars. Hence, it implicates the zone of deposition for hydrocarbon‐bearing fluvial facies. Based on the porosity constraint, the gas‐bearing fluvial reservoirs have high (>25%) porosity, which implicates the development of aggradational parasequences. The oil‐dominated sandstone facies experience relatively low‐porosity of <12% and negligibly high density and high velocity, which implicates the development of transgressive parasequences. The reservoir system shows an eastward shale‐out zone, where the high‐density and high‐velocity shales mask the channel‐levee systems. Consequently, these stratigraphic configurations are developing into a cost‐effective stratigraphic trap, and hence, this exploration scheme serves as an analogue for global fluvial depositional systems.

Multi-fluorine labeled indanone derivatives as potential MRI imaging probes for β-Amyloid plaques.

In order to realize the early diagnosis of Alzheimer's disease (AD), we designed and synthesized a series of multi-fluorine labeled indanone derivatives based on indanone which could target β-amyloid (Aβ). Through the in vitro staining experiment and affinity experiment, we selected 7d out, and then evaluated it through other in vivo and in vitro experiments. The staining of AD human brain adjacent sections revealed that compound 7d could bind to Aβ plaques with high affinity. In the in vitro binding assay, 7d showed a balanced affinity with Aβ1-40 (Kd = 367 ± 13) and Aβ1-42 (Kd = 384 ± 56). Also, 7d exhibited a low toxicity (LD50 > 50 mg/kg) and an excellent ability to pass through the blood-brain barrier (Log p = 3.87). The biodistribution experiment in mice showed that 7d reached the highest brain uptake after 1 h of tail vein injection and cleared after 24 h. A low concentration of 7d (1.875 mg/ml) showed a strong imaging ability (19F-weighted mode), and the imaging capability increased with the increasing of concentration. All the results showed that 7d could provide a feasible solution for the early diagnosis of AD under non-radioactive condition.

Monitoring residual fouling after cleaning of multi-fiber membrane modules fiber-by-fiber using non-invasive MRI monitoring

In this study non-invasive low field magnetic resonance imaging (MRI) technology was used to monitor fouling induced changes in fiber-by-fiber hydrodynamics inside a multi-fiber hollow fiber membrane module containing 401 fibers. Using structural and velocity images the fouling evolution of these membrane modules were shown to exhibit distinct trends in fiber-by-fiber volumetric flow, with increasing fouling causing a decrease in the number of flow active fibers. This study shows that the fouling rate is not evenly distributed over the parallel fibers, which results in a broadening of the fiber to fiber flowrate distribution. During cleaning, this distribution is initially broadened further, as relatively clean fibers are cleaned more rapidly compared to clogged fibers. By tracking the volumetric flow rate of individual fibers inside the modules during the fouling-cleaning cycle it was possible to observe a fouling memory-like effect with residual fouling occurring preferentially at the outer edge of the fiber bundle during repeated fouling-cleaning cycle. These results demonstrate the ability of MRI velocity imaging to quantitatively monitor these effects which are important when testing the effectiveness of cleaning protocols due to the long term effect that residual fouling and memory-like effect may have on the operation of membrane modules.

Core−shell structured hollow copper sulfide@metal−organic framework for magnetic resonance imaging guided photothermal therapy in second near-infrared biological window

Multifunctional core-shell hybrids formed by integration of metal-organic framework (MOF) and functional materials have attracted extensive attention as promising theranostic nanoplatforms due to their combined novel properties and enhanced therapeutic efficacy. Recently, the second near-infrared (NIR-II, 1000–1700 nm) laser-induced photothermal therapy (PTT) as compared to the NIR-I(700–950 nm) laser-induced PTT has displayed improved therapeutic effects owing to its merits that include deeper tissue penetration and increased maximum permissible exposure. Herein, a novel core-shell hollow copper sulfide@metal−organic framework (HCuS@MIL-100) has been successfully fabricated by a layer-by-layer technique for the first time and their collective theranostic effects are investigated in vitro and in vivo. In this platform, the inner HCuS was applied as the NIR-II photothermal agent with excellent NIR-II absorption feature, leading to impressive photothermal effects under irradiation by 1064 nm light. With MIL-100 as the shell, HCuS@MIL-100 not only displayed optimal biocompatibility but also presented superior T2 magnetic resonance imaging (MRI) ability. In the current study multifunctional hollow core-shell HCuS@MIL-100 are fabricated for the MRI-guided PTT. This study also offers a facile and effective strategy for the development of novel theranostic platforms with high efficiency through the integration of MOFs and functional materials.

A Facile Structural Isomerization‐Induced 3D Spatial D‐A Interlocked Network for Achieving NIR‐II Phototheranostic Agents

AbstractThe performances of second near‐infrared (NIR‐II) organic phototheranostic agents (OPTAs) depend on both molecular structure and molecular packing when used as nanoparticles (NPs). Herein, we proposed a facile structural isomerization‐induced 3D spatial donor (D)‐acceptor (A) interlocked network for achieving NIR‐II OPTAs. Two isomers, 4MNVDPP and 6MNVDPP were synthesized and formulated into NPs. 6MNVDPP, which has a larger electrostatic potential difference, exhibits a compact 3D spatial D‐A interlocked network in the crystal form, while 4MNVDPP forms 2D D‐D type J‐aggregates. Thus, 6MNVDPP NPs show red‐shifted NIR absorption and larger molar extinction coefficient than 4MNVDPP NPs. Thanks to the typical NIR‐II emission, superior photothermal‐stability, high photothermal conversion efficiency (89 %) and reactive oxygen species production capacity, 6MNVDPP NPs exhibit outstanding NIR‐II tiny capillary vasculature/tumor imaging ability and synergistic photothermal/photodynamic anti‐cancer effect in vivo.