Ilha Das Research Articles

A simple PCR-RFLP method for the discrimination of native and introduced oyster species (Crassostrea brasiliana, C. rhizophorae and C. gigas; Bivalvia: Ostreidae) cultured in Southern Brazil

Aquaculture ResearchVolume 37, Issue 15 p. 1598-1600 A simple PCR-RFLP method for the discrimination of native and introduced oyster species (Crassostrea brasiliana, C. rhizophorae and C. gigas; Bivalvia: Ostreidae) cultured in Southern Brazil Marcio R Pie, Marcio R Pie Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorRaphael O Ribeiro, Raphael O Ribeiro Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorWalter A Boeger, Walter A Boeger Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorAntonio Ostrensky, Antonio Ostrensky Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorRenan M Falleiros, Renan M Falleiros Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorLeandro Angelo, Leandro Angelo Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this author Marcio R Pie, Marcio R Pie Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorRaphael O Ribeiro, Raphael O Ribeiro Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorWalter A Boeger, Walter A Boeger Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorAntonio Ostrensky, Antonio Ostrensky Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorRenan M Falleiros, Renan M Falleiros Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this authorLeandro Angelo, Leandro Angelo Departamento de Zootecnia, Universidade Federal do Paraná, Curitiba, PR, Brazil Grupo Integrado de Aqüicultura e Estudos Ambientais, Universidade Federal do Paraná, Curitiba, PR, BrazilSearch for more papers by this author First published: 28 September 2006 https://doi.org/10.1111/j.1365-2109.2006.01591.xCitations: 23 Correspondence: M R Pie, Departamento de Zoologia, Caixa Postal 19073, Universidade Federal do Paraná Curitiba, PR 81531-990, Brazil. E-mail: pie@ufpr.br Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume37, Issue15November 2006Pages 1598-1600 RelatedInformation

Ascídias introduzidas na Baía de Paranaguá, Paraná, sul do Brasil

Exotic (introduced) species are a growing problem in ports worldwide and comprise the most important impacts in marine ecosystems. Periodic monitoring to detect introduced species is extremely important for effective population control. Here we sampled ascidian species near the port of Paranagua for a taxonomic study of this fauna to attempt to detect introduced species. Larval stages in ascidians are short-lived, and dispersal is restricted to small distances, and so ascidians are very good bioindicators for exotic introductions due to ship transport. Four locations were sampled within Paranagua Bay (Ilha das Cobras, Pier Tenenge, Ilha do Mel and Ilha da Galheta) and one location outside of the bay (Parque dos Meros). Information for the nearby fauna and for geographic distributions of the species involved was obtained from the literature. Eighteen species were found: Perophora multiclathrata (Sluiter, 1904), Ascidia curvata (Traustedt,1882), A. sydneiensis Stimpson, 1855, Clavelina oblonga Herdman, 1880, Cystodytes dellechiajei (Della Valle, 1877), Eudistoma carolinense van Name, 1945, Distaplia bermudensis van Name, 1902, Didemnum granulatum Tokioka, 1954, Diplosoma listerianum (Milne-Edwards, 1841), Lissoclinum fragile (van Name, 1902), Botryllus planus (van Name, 1902), B. tuberatus Ritter & Forsyth 1917, Botrylloides nigrum Herdman, 1886, Symplegma rubra Monniot, 1972, Styela canopus (Savigny, 1816), S. plicata (Lesueur, 1823), Microcosmus exasperatus Heller, 1878 and Molgula phytophila Monniot, 1970. The known geographic distributions based on the literature and collections suggest that three species are native, one is a inter-regional introduction, two are introduced from the Pacific and the remaining 12 are cryptogenic.

Introduced ascidians in Paranaguá Bay, Paraná, southern Brazil

Exotic (introduced) species are a growing problem in ports worldwide and comprise the most important impacts in marine ecosystems. Periodic monitoring to detect introduced species is extremely important for effective population control. Here we sampled ascidian species near the port of Paranaguá for a taxonomic study of this fauna to attempt to detect introduced species. Larval stages in ascidians are short-lived, and dispersal is restricted to small distances, and so ascidians are very good bioindicators for exotic introductions due to ship transport. Four locations were sampled within Paranaguá Bay (Ilha das Cobras, Pier Tenenge, Ilha do Mel and Ilha da Galheta) and one location outside of the bay (Parque dos Meros). Information for the nearby fauna and for geographic distributions of the species involved was obtained from the literature. Eighteen species were found: Perophora multiclathrata (Sluiter, 1904), Ascidia curvata (Traustedt,1882), A. sydneiensis Stimpson, 1855, Clavelina oblonga Herdman, 1880, Cystodytes dellechiajei (Della Valle, 1877), Eudistoma carolinense van Name, 1945, Distaplia bermudensis van Name, 1902, Didemnum granulatum Tokioka, 1954, Diplosoma listerianum (Milne-Edwards, 1841), Lissoclinum fragile (van Name, 1902), Botryllus planus (van Name, 1902), B. tuberatus Ritter & Forsyth 1917, Botrylloides nigrum Herdman, 1886, Symplegma rubra Monniot, 1972, Styela canopus (Savigny, 1816), S. plicata (Lesueur, 1823), Microcosmus exasperatus Heller, 1878 and Molgula phytophila Monniot, 1970. The known geographic distributions based on the literature and collections suggest that three species are native, one is a inter-regional introduction, two are introduced from the Pacific and the remaining 12 are cryptogenic.

Assessing the potential toxicity of marine sediments found in petroleum industry areas: A new approach based on responses of postlarval shrimp

In this study we tested the toxicity of bulk sediment from the northeastern area of Todos os Santos Bay, Bahia, Brazil, to evaluate environmental impact induced by 50 years of exposure to the local petroleum industry (Petrobras). Sediment samples were collected during one year, at three-month intervals, from four sites in areas of oil extraction (Ilha das Fontes, station 4), transportation (Ilhas de Madre de Deus and Pati, stations 2 and 3) and refinement (RELAM, station 1). Two reference stations (5 and 6) were located outside the petroleum influence area, to the south of the bay. Static bioassays were conducted for 96 h, using 7–8 day old Litopenaeus vannamei postlarvae (PL). The assays were conducted in 2.5 L plastic jars containing 200 g of surface (1 cm deep) bulk sediment covered by 2 L of dilution water (filtered seawater, 28 ppt salinity, 27 + 2ºC and DO under saturation). Fifteen exposed PL in each jar were fed daily on 60 recently hatched Artemia salina nauplii. Physico-chemical parameters were monitored. Mortality and dry weight gain were taken as end-points. The PL mortality data obtained for sediment from the Petrobras stations in comparison to the data from the reference stations were not significantly different (P > 0.05); however, the dry weight gain showed significant differences among stations. A maximum value was reached at station 5 (reference area) and a minimum at station 1 (RELAM refinery). Stations 2 and 3 in petroleum transportation areas did not show significant differences (P > 0.05). To evaluate the sensitivity of this bulk-sediment test in detecting contaminant effects generated by the petroleum industry, the toxicity data were considered in terms of the hydrocarbon levels analyzed in sediments from the same Petrobras areas and in one of the control areas, located outside the bay. The results support the assumption that the bulksediment bioassay on penaeid PL is a suitable methodology not only to distinguish between impacted and relatively unperturbed environments, but also to separate the different degrees of impact among areas subjected to petroleum industry activities in the coastal environment.

Epidemiological aspects of filariosis in dogs on the coast of Paraná state, Brazil: with emphasis on Dirofilaria immitis

The present study determined the prevalence and geographical distribution of Dirofilaria immitis and other filariae, from dogs in littoral areas of Paraná state, in Brazil. This survey spanned eight months, between 1998 and 1999, and was also designed to compare the efficacy of different tests for diagnosis of heartworm infection in that area. Blood samples were collected from 256 native-owned dogs distributed along the Paraná coastal area. Five diagnostic procedures were used: direct smear examination, the Knott’s modified test, filtration assay, and two heartworm antigen detection kits. A follow-up imaging exam was performed to support the heartworm diagnosis. The imaging diagnosis included radiographic and ultrasonographic exams of six dogs that had positive results for the heartworm antigen detection kits, but showed different microfilarial burdens. The presence and severity of radiographic and ultrasonographic signs were compared with the results obtained in microfilariae detection and antigen tests. Diagnostic parasitology results indicated that 31.25% of the dogs were microfilaremic. Three different microfilariae were recovered: D. immitis, Dipetalonema reconditum, and the third (mf3) was not identified. D. reconditum was the species with the highest prevalence: 22.6%. In general, D. immitis prevalence was 5.47% (28.57% occult infections), but it varied along the coast and the range was from 0 to 20%. No correlation could be established between the overall scores for microfilarial counts (small or large numbers) and the severity of radiographic results or the likelihood of detecting filariae in the pulmonary artery using echocardiography. The finding of a different type of microfilaria (mf) suggested the existence of a third species in Paraná state, whose prevalence was 4.68%. These results show that to obtain a reliable diagnosis of heartworm infection, antigen detection kits are indicated. Knott’s test or filtration should be performed to confirm microfilaremia and not for diagnosis of heartworm infection. Imaging tests support parasitology exams and add more about severity of infection. The northern areas, specially Guaraqueçaba and Ilha das Peças, presented the highest number of heartworm-infected dogs.

The grammitidaceae, pteridophyta, of macaronesia

AbstractGrammitis marginella (Sw.) Sw. is reported from the Azores, Portugal, and thus for the first time from Europe. The Azorean plants are described as subsp. azorica subsp. nova. A key for the three Macaronesian species of the family Grammitidaceae (C.PRESL) CHING is presented. Studies on the ecology of G. marginella and the related Ceradenia junger‐mannioides (KLOTZSCH) L.E.BISHOP on Ilha das Flores, Azores are reported.

A associação entre Crepidula aculeata (Gastropoda, Calyptraeidae) a alga calcárea Amphiroa beauvoisii, na Baía de Santos, São Paulo, Brasil

Association Crepidula aculeata - Amphiroa beauvoisii. Population study of Crepidula aculeata Gmelin, 1791) (Gastropoda Calyptraeidae) and analysis of protocooperative association between the mollusc and the alga were carried out in the phytal of calcareous alga Amphiroa beauvoisii Lamouroux, 1816 at Ilha das Palmas, Santos Bay (24o00'S - 46o19'W). Samples were collected at three sites of different degree of water movement in the intertidal zone and sublittoral fringe during 1975 and 1976. From 93,916 organisms belonging to 172 species, 5,331 were Crepidula, the most abundant among sessile species. Also, Crepidula constitutes a substratum to attachment of Amphiroa, accounting for 30% of total phytal area. There was a positive correlation between the growth of shell and epizoic alga. Higher level of the phytal belt was more suitable for attachment and/or growth of the epizoic alga, without influence from water movement or season of the year. This association depends on the external morphology of the shell, filter-feeding habit, high adhesive power and lacking of mechanisms avoiding attachment of epizoics of Crepidula. Exuberant growth of the epizoic tuft over Crepidula female shell would constitute camouflage and protection of offsprings against predators.

Guinea Bissau – Khmer Republic – Middle East – Latin America

On 31 July an ICRC delegate and a doctor-delegate visited, on the Ilha das Galinhas, 33 members of the PAIGC (African Party for the Independance of Guinea and Cape Verde) to whom the Portuguese Government had granted prisoner-of-war status. The following day, the delegates went to see a sick prisoner being treated at the hospital in Bissau.