IgM Seroprevalence Research Articles

Seroprevalence and Risk Factors of Toxoplasmosis in Togo

Objectives: Toxoplasmosis is caused by Toxoplasma gondii. 50 to 70% of African are contaminated and 60% to 80% in Togo. This parasitic infection involves disorders in immunocompromised persons and pregnant women. The purpose of this study is to evaluate the seroprevalence of toxoplasmosis and the risk factors of contamination in the Togolese population. Methods: Prospective studies from January 2012 to December 2015 concerned 7076 patients. A survey on the risk factors for Toxoplasma gondii contamination has done. The seroprevalence was monitored by examining serological antibodies IgG and IgM using ELISA method. Sampling of 200 excreta of domestic cats were analyzed by coproscopy examinations Results: The average age was 32.61±6.80 years old. The seroprevalence of IgM was 2.85%; and for IgG 59.7%. IgG were high in men (67.0% vs. 58.6%, p<0.0001); IgM were high in women (2.81% vs. 1.85%, p<0.01). In this study, we observed 57.49% immunized subjects. Regarding potential risk factors for contamination, the survey revealed that 80.5% of subjects consume raw garden produce, 65.4% non-potable and 8.3% undercooked meat. In cats, 20.5% of them excreted oocysts of Toxoplasma gondii. Conclusion: The prevalence of the toxoplasmosis was high in the Togolese population because the risks factors were variable and the level of contamination were high too. The country’s health authorities, especially for the pregnant women, should install sensitization and prevention programs on the risks of the contamination of toxoplasmosis. The high proportion of the young subjects affected constitutes a socio-economical danger for the country.

Seroprevalence of HCMV among Pregnant Women and Its relation to CD4 and CRP

The HCMV is a widespread viral pathogen characterized by strict host specificity and is limited to humans. It has been described as an important etiological agent of intrauterine infection in during the pregnancy, which may lead to some serious results such as miscarriage, cerebellar malformation stillbirth, and fetus developmental retardation. The study carried out in Kirkuk governorate from the December 2017 to May 2018 for study the relation of CD4 percentage and CRP with HCMV seropositive pregnant women. The number of pregnant women under study was two hundred women attending to some private medical laboratories in Kirkuk. The pregnant women were examined for the seroprevalence of HCMV IgM and IgG antibodies by using VIDAS technique. The results were (81 %), (9%) and (6%) for HCMV-IgG, HCMV-IgM and for both IgG & IgM at the same time respectively. The highest rates (41.66%) of decreased CD4 percentage were within seropositive pregnant women for both IgG & IgM at same time, while the highest rates (16.66%)of CRP positive were found within HCMV-IgM seropositive group.

Seroprevalence of cytomegalovirus antibodies positivity among voluntary blood donors in the Delta State University Teaching Hospital (DELSUTH), Oghara, and its environs in Delta State - Nigeria

Background: Cytomegalovirus (CMV) is widely distributed globally and it is found in different geographical and socioeconomic groups. It can be transmitted from person to person via blood transfusion or tissue/organ transplantation and if the receipt is immune-compromised, then a severe infection and mortality can occur.
 Study Objective: The purpose of this study is to determine the seroprevalence and risk factors for Human Cytomegalovirus (HCMV) among blood donors in DELSUTH and its environs.
 Materials and Methods: This is a randomized cross-sectional study of voluntary blood donors for the purpose of replenishing stock in the blood bank system of the Delta State University Teaching Hospital, Oghara-Delta State, Nigeria and also from outreach blood donation centres around DELSUTH during blood donation campaigns. Five millilitres of venous blood was taken from each voluntary blood donor and assayed for CMV IgG and IgM using enzyme linked immunosorbent assay.
 Results: A total of 185 voluntary donors were recruited for this study comprising of 162 males and 23 females. The age of the donors ranges from 23 to 62years. Human CMV IgG seroprevalence rate for males is 93% and for the females 91%. Therefore the overall CMV IgG seroprevalence rate is 92%. The CMV IgM seroprevalence rate is zero percent. There is no significant difference between the seroprevalence rates in respect of their gender, age, occupation, level of education and previous history of blood transfusion. (p>0.5)
 Conclusion: The rate of seroprevalence of CMV is high among the studied population and this call for screening of voluntary-donor's blood before transfusion especially to those that are immunocompromised.

Seroprevalence of hepatitis E among restaurant food handlers in Ibadan, Nigeria

Background: Hepatitis E virus (HEV) is one of the causative agent of acute viral hepatitis in humans. HEV is an important public health disease in many parts of the world because it is transmitted faeco-orally. Majority of the documented studies on hepatitis E virus in Nigeria have focused on pregnant women and animal handlers with limited data among food handlers. Thus the current study aimed at investigating the prevalence of HEV infection among food handlers operating within the premises of a tertiary care facility.Methods: One hundred and seventy seven (177) food handlers were screened using commercial Enzyme-Linked Immuno-Sorbent Assay (ELISA) to detect IgM antibodies to Hepatitis E. A semi-structured questionnaire was used to assess risk factors for HEV infection.Results: HEV IgM antibodies were detected in 16 (9.0%) of the participants. Age-specific HEV IgM seroprevalence appeared to decrease with age, however there were no significant differences in HEV IgM seropositivity regarding age (P=0.251), gender (P = 0.231), marital status (P=0.735) and religion (P = 0.906). Significant risk factors for HEV IgM seropositivity included source of water for drinking (P=0.03) and the use of soap for hand washing (P=0.02).Conclusion: Our findings suggest that HEV remains a public health problem, as the virus circulates at low but considerable levels especially among food handlers; thus posing a threat to potential contacts. Proper hand washing practices as well as provision of portable water are important factors for the control of Hepatitis E.

PUBLIC HEALTH IMPLICATIONS OF TOXOPLASMOSIS IN ANIMALS AND WOMEN IN SELECTED LOCALITIES OF MENOUFIA GOVERNORATE, EGYPT

Toxoplasma gondii is an important protozoan with serious obstetric impact in animals and women. The work aimed to relate public health significance of toxoplasmosis among some animals and women in Menoufia Governorate. A total 50 blood samples were collected from 50 adult sheep (30 from Kafr Tanbidi and 20 from Shebein El-Kom) and a total of 31 cattle milk samples were collected from different localities in Shebein El-Kom. Sheep sera were examined by ELISA IgG and recorded seropositivity of 60% (30/50) with 66.67% (20/30) were positive in Kafr Tanbidi and 50% (10/20) in Shebein El-Kom. PCR on sheep blood recorded 22% (11/50), of them 20% (6/30) were positive in Kafr Tanbidi and 25% (5/20) in Shebein El-Kom while PCR on cattle milk revealed 19.35% (6/31) positive for Toxoplasma DNA. On the other hand, Toxoplasma seroprevalence among 250 women was 54.4% (136/250) by ELISA IgG and 8.4% (21/250) by ELISA IgM. There was a significant association between IgG seroprevalence and age, increased gravidity, abortion, climate, cat contact, consumption of raw cow milk, undercooked meat and contaminated water from wells and between IgM seroprevalence and age, abortion and climatic conditions prevailed during sampling.

Seroprevalence of Anti-Toxoplasma IgG and IgM among Pregnant Women of Shush County, Southwest of Iran

Background: Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis. T. gondii can cause serious pathological effects in the fetus of infected women. Objectives: The aim of this study was to investigate the seroprevalence of anti-Toxoplasma IgG and IgM among pregnant women of Shush county, southwest of Iran. Methods: This descriptive cross-sectional study was conducted in 276 pregnant women. IgG and IgM antibodies of T. gondii were measured by the Enzyme-linked immunosorbent assay (ELISA) test. SPSS version 21 statistical software was used for data analysis and Chi-square test for significant differences. Results: Of 276 women, 85 (30.8%) and 2 (0.7%) were positive for IgG and IgM, respectively. Of 146 urban women, 43 (29.5%) were positive for IgG and of 130 rural women, 42 (32.3%) were positive for IgG. There was no significant difference between rural and urban women for IgG antibody (P > 0.05). Also, 2 (1.4%) out of 146 urban women were positive for IgM. All of the 130 rural women were negative for IgM. No significant difference was found between rural and urban women for IgM antibody (P > 0.05). Conclusions: The findings indicated there is a relatively high prevalence of T. gondii in the study pregnant women. Therefore, evaluation of T. gondii before marriage and pregnancy is important for women and the health authorities of Shush County must pay more attention to control and prevention of the transmission of the parasite to individuals.

Seroprevalence of acute human parvovirus B19 viraemia among anaemic children in ibadan city, Nigeria

Objective: To determine the seroprevalence of B19V IgM as a measure of acute infection and associated risk factors among 0.05). Conclusions: The seroprevalence of 14.7% among anaemic children confirm that these infections are endemic in Nigeria. This level of infectivity suggests that there is a high risk of transmission to healthy children as well as children with underlying haemolytic or acquired anaemia in Nigeria.

EPIDEMIOLOGICAL STUDY ON THE ROLE OF QUAILS IN TRANSMITTING OF TOXOPLASMA GONDII TO MAN

This study aimed to detect Toxoplasma gondii in quail samples collected from different houses in Sohag Governorate by histopathological examination and determine the seroprevalence of IgM and IgG in aborted women using ELISA test. The results revealed that 10% of quail samples were positive for Toxoplasma gondii, 48.9 % of aborted women were seropositive for IgM (5.4%), IgG (38%) and both IgM and IgG (5.4%). Higher infection rate was reported in age group ranging from18 to 20 years, in women with first time of abortion and in women live in rural areas. This study highlights on the role of quail as a source of Toxoplasma infection for human.

Serologic and viral genome prevalence of HSV, EBV, and HCMV among healthy adults in Wuhan, China.

The worldwide infection rate of herpesvirus is high, but the detailed prevalence in China, especially the central area, remains unclear. In the present study, the prevalence of herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a representative city in Central China. Viral-specific IgG and IgM titers were examined in the serum by chemiluminescent immunoassay, and the existence of viral genomic DNA in blood cells was determined by nested PCR. The overall IgG seroprevalences were 81.5%, 95.4%, and 93.7% for HSV, EBV, and HCMV, while the corresponding IgM seroprevalences were only 6.3%, 2.3%, and 0. The viral genomic DNA of HSV, EBV, and HCMV was identified in the blood samples of 5.9%, 14.2%, and 22.8% of the tested donors, respectively. Significantly, less HSV IgM-positive samples were found in the population over 20 years old than below 20 group; female displayed higher chances for HSV IgG and genome positivity; and occupations such as waiters and medical staffs were shown to be with higher risk for HCMV genome positivity. This study provided useful reference data for the HSV, EBV, and HCMV prevalence in central China, and suggested the potential importance of detecting viral genome to complement serum test data.

Seroprevalence of IgG, IgM and IgA against Toxoplasma gondii in pregnant women in first trimester in northern Khorasan Province, Iran

Seroprevalence of IgG, IgM and IgA against Toxoplasma gondii in pregnant women in first trimester in northern Khorasan Province, Iran

Rubella virus infection and associated factors among pregnant women attending the antenatal care clinics of public hospitals in Hawassa City, Southern Ethiopia: a cross-sectional study

ObjectiveTo assess the seroprevalence of recent/acute and past exposure to rubella virus infection and associated risk factors among pregnant women.DesignA hospital-based cross-sectional study.SettingThe study was conducted in two public hospitals...

Seroprevalence of Human Herpesvirus Type 2 in a Reference Center for Pregnant Women in Rio de Janeiro, Brazil

Introduction: Pregnant women stand as an relevant group for research about Human Herpesvirus (HHV-2) infection owing to the risk of mother-to-child transmission. Methods: Women attending in a prenatal care center were tested for HHV-2 IgM and IgG by ELISA. Quantitative PCR test was the chosen method to ascertain viremia. Results: The seroprevalence of IgG and IgM anti-HHV-2 was 20.6% and 2.2% respectively. HHV-2 viremia was found in one pregnant woman with HHV-2 IgM, leading to the assumption of primary infection. Conclusion: The significantly high prevalence of HHV-2 found and the ascertainment of primary infection in a pregnant woman underline the need for constant HHV-2 follow-up and diagnosis in order to avoid sexual transmission.

Seroprevalence of anti-Chlamydia trachomatis IgM in neonatal respiratory tract infections in Hungary.

Purpose. To determine the seroprevalence of specific IgM indicative of respiratory tract infection (RTI) due to Chlamydia trachomatis (CT) among symptomatic infants.Methodology. A descriptive study was conducted on young infants up to 5 months old at the Bacterial Sexually Transmitted Infections Reference Laboratory, National Centre for Epidemiology, Budapest, covering the period 2008-2016. Serum samples from infants suffering from RTIs were screened with a micro-immunofluorescence test (Focus, Cypress, USA) for the presence of anti-Chlamydia trachomatis-specific IgM. A parallel Bordetella pertussis screening was performed by an indirect immunofluorescence test (Euroimmun, Lübeck, Germany) that detected specific IgM.Results.The CT-specific serum IgM was highly reactive in 50 (19.1 %) of the 262 neonates with RTIs, while all proved negative for Bordetella pertussis-specific IgM.Conclusion. Vertically transmitted C. trachomatis must be regarded as a common pathogen among symptomatic neonates with RTIs in Hungary. Routine screening and treatment of pregnant women could be one option to help prevent these conditions. Focused laboratory testing based on raised clinical awareness should enable early diagnosis and appropriate therapy for symptomatic infants.

Cytomegalovirus Infection among Pregnant Women in Beijing: Seroepidemiological Survey and Intrauterine Transmissions.

Primary cytomegalovirus (CMV) infection during pregnancy can cause congenital defects. Available data for CMV infection during pregnancy in north China are inadequate. The aim of this study was to evaluate the epidemiology of maternal CMV infection and explore the incidence of congenital infection. In this prospective study, serum CMV IgG and IgM antibodies were measured in 2,887 pregnant women using ELISA, and the IgG avidity test was performed on all IgM-positive subjects. The seroprevalence of anti-CMV IgG was 94.70%, and of anti-CMV IgM was 1.28%. CMV IgG prevalence increased significantly with age (p < 0.01). Women living in downtown areas showed higher IgG prevalence than those residing in urban areas (p = 0.023). CMV-IgM seroprevalence was highest in autumn (p = 0.021). There was no difference in IgM seroprevalence by age, socioeconomic status, geographical area, or gravida. The rate of primary CMV infection was 0.45% (13/2,887) at the first trimester. The seroconversion rate during pregnancy was 0.76% (22/2,887). One woman underwent seroconversion during pregnancy and gave birth to an infant with asymptomatic CMV infection. Congenital CMV infection was diagnosed in five of the 14 infants from 14 mothers with active infection, for a vertical transmission rate of 35.71% (5/14). Three infants were asymptomatic, whereas two infants presented symptomatic infection with hearing deficits. Although CMV IgG prevalence is relatively high in north China, significant attention to primary CMV infection during pregnancy is still needed.

Seroprevalence and Potential Root Cause of Toxoplasmosis in Primary Children : A Study Conducted at King Abdulaziz Hospital

Background: Toxoplasmosis is a zoonosis with a high prevalence throughout the world. Toxoplasma gondii infection is frequently asymptomatic. Primary school children are particularly vulnerable to toxoplasmosis due to their habits of playing in water, soil, eating various raw foods, or contact with pets, including dogs, cats, and birds and hence they are an ideal target group to investigate T. gondii prevalence. Data collected from this age group can thus be used to assess whether T. gondii threatens the health of school-aged children, and also as a reference for evaluating the need for community interventions. The aim of this study was to determine the prevalence rate of anti-toxoplasmosis IgG and IgM in Primary School children and to detect agents that increase prevalence of the disease. Materials and methods: 328 primary children (219 females and 109 males) were screened for T. gondii antibodies with ELISA along with a questionnaire conducted to all children and parents/guardians to obtain data for relevant eating and social habits. Among the risk factors tested, including contact with cats and soil, consumption of raw meat and vegetables, and drinking unboiled milk. Results: the seroprevalence of anti-Toxoplasma IgG & IgM was found to be 12 % (40 out of 328) and 2.4 % (8 out of 328), respectively while combined anti Toxoplasma IgG & IgM antibodies were found in 1.5 % of the children (5 out of 328). Infection acquisition rate was directly proportional to age and it was of interest that the most significant risk factor was not the petting of cats but the ingestion of raw meat. The seroprevalence of T. gondii IgG & IgM by ELISA among primary children in this region of Saudi Arabia is considerable with few identifiable significant risk factors reported. Conclusion: effective measures should be taken to prevent and control T. gondii infection in primary school children, the results of this study showed that in order to increase awareness of the disease in the community especially for girls and their parents on common root causes such as raw meat and vegetable is necessary. Policy makers also need to initiate prevention and control programs to not only primary children but also pregnant women and immunocompromised patients in particular because they are more severely affected by T. gondii infection.

Assessment of Toxoplasma Seropositivity in Children Suffering from Attention Deficit Hyperactivity Disorder

Background: Toxoplasmosis, a protozoan infection caused by Toxoplasma gondii, is suggested to be a risk factor for many psychological disorders such as schizophrenia and depression. Attention deficit hyperactivity disorder (ADHD) is one of the most common psychiatric disorders among children. Previous studies have assessed the correlation between ADHD and infectious diseases like toxoplasmosis. So we aimed to investigate the possible correlation between Toxoplasma seropositivity and ADHD in children. Materials and Methods: In this cross-sectional study children with signs of ADHD were regarded as patients and underwent clinical assessments. Diagnosis of these patients was based on DSM-IV-TR system which was performed by a pediatric psychiatrist. Forty-eight Children without signs of ADHD or other psychic disorders were considered as control, and 48 patients were considered as case group. Parents were asked to answer a questionnaire including demographic, pregnancy and habitual questions. Blood samples were taken from all individuals and assessed for anti-Toxoplasma IgM and IgG antibodies. Results: Ninety-six male and female patients with a mean age of 8.12 years underwent analysis. Seropositivity rate for anti-T.gondii IgG antibody was 4.2% in the case and 2.1% in control individuals (P=0.92). Anti-T.gondii IgM antibodies were not found in control individuals while it was found in 2.1% of case individuals (P=0.74). There was no statistically significant association between seroprevalence of IgM (P=0.74) and IgG (P=0.92) antibodies and ADHD in study individuals. Conclusion: Our findings showed that the toxoplasmosis seropositivity has no significant difference between children with and without ADHD. Further studies are needed with a larger amount of individuals. [GMJ. 2016;5(4):188-93]

Imunski status trudnica u odnosu na najčešće uzročnike kongenitalnih infekcija u Republici Srpskoj / The immune status of pregnant women in relation to the most common cause of congenital infection in the Republic of Srpska

Obligatory TORCH (Toxoplasma gondii, Rubella virus, Citomegalovirus, Herpes simplex virus) testing is provided to every pregnant woman in developed countries. This test is measuring serums level of IgM antibody (sign of acute infection) and protective IgG antibody. There is no such obligatory testing in Republic of Srpska, although is the only method for early detection and prevention of TORCH infection. Our aim was to determine seroprevalence of Rubella virus, Citomegalovirus i Herpes simplex virus in pregnant women, their vaccinal status and fequence of complications in previuos pregnancies.Sera were collected from pregnant women during their antenatal visit to outpatients departments of gynecology in general hospitals in Doboj, Prijedor and Banja Luka. All pregnant women answer. Sera were frozen and testing was preformed using Elysis Duo (Human, Germany), fully automated ELISA analyzer. Statistical analysis was realized using the SPSS software package, along with the level of statistical significance of 5% (α=0.05).During six-month period (January 2008 to July 2008) 641 pregnant women were examined. Blood samples were obtained from 320, 217 and 158 women in the first, second and third trimesters of gestation, respectively. The IgG seroprevalence was 62.61 % for Rubella virus, 84.87% for CMV and 84.40% for HSV. The IgM seroprevalence was 0.16% for Rubella virus, 1.40% for CMV and 6.24% for HSV.The IgG seroprevalence for Rubella virus is not satisfactory, because of unregular vaccination during the civil war. Seroprevalences for Citomegalovirus and Herpes simplex virus are satifactory, as for seroprevalence in developed countries.

Sociodemographic factors associated with IgG and IgM seroprevalence for human cytomegalovirus infection in adult populations of Pakistan: a seroprevalence survey.

BackgroundThe seroprevalence of human cytomegalovirus (HCMV) infection ranges from 30 to 90 % in developed countries. Reliable estimates of HCMV seroprevalence are not available for Pakistan. This study determined the seroprevalence and sociodemographic factors associated with HCMV infection in adult populations of Karachi, Pakistan.MethodsA seroprevalence survey was conducted on 1000 adults, including residents of two semi-urban communities, and visitors to a government and a private hospital. Questionnaire-based interviews were conducted. Sera were analysed for HCMV-specific IgG and IgM. Chi-square or Fisher’s exact test was used for comparing sociodemographic variables against seropositivity of HCMV-IgG or IgM. Multiple logistic regression modeling was performed for IgG seroprevalence and adjusted odds ratios were computed.ResultsThe seroprevalence of HCMV-IgG and IgM was 93.2 and 4.3 % respectively. 95.3 % of individuals who were IgM seropositive were also seropositive for IgG. Around 6 % (15/250) of women of childbearing age remained uninfected and were therefore susceptible to primary infection. HCMV-IgG seroprevalence was associated with being female (p = 0.001), increasing age (p = 0.002) and crowding index (p = 0.003) and also with lower levels of both education (p < 0.001) and income (p = 0.008). Seroprevalence also differed significantly by marital status (p = 0.008) and sampling location (p < 0.001). A logistic regression model for HCMV-IgG seroprevalence showed associations with being female (OR = 1.89; 95 % CI: 1.10–3.25), increasing age (OR = 3.95; 95 % CI: 1.79–8.71) and decreasing income (OR = 0.72; 95 % CI: 0.54–0.96). A strong association was observed between increased seroprevalence of HCMV-IgM and decreasing household size (p = 0.008).ConclusionsSeroprevalence of HCMV is very high in Pakistan, although 6 % of women of childbearing age remain at risk of primary infection. The IgM seropositivity observed in some individuals living in small household size (1–3 individuals) with persistent HCMV infection could have resulted from a recurrent HCMV infection. Future longitudinal research in pregnant women and neonates is required to study the trends in HCMV seroprevalence over time in Pakistan for the development of a potential HCMV prevention and vaccination programme.

Seroprevalence of Cytomegalovirus Antibodies and Primary Infection among Women and Infants in Iran: A Meta-Analysis

Background: Seroprevalence of Cytomegalovirus infection varies between 40% and 100% worldwide. Different studies carried out in Iran indicate this variation in this country. It is important to estimate the total infection prevalence using a reliable method such as meta- analysis in order to be applied by policymakers. This study aims to estimate the IgG and IgM seroprevalences of CMV infection among Iranian women and neonates. Method: We selected eligible articles for final meta- analysis by searching the national and international databases, excluding duplicates and irrelevant papers from primarily identified studies after abstract/full text review, implementing exclusion/inclusion criteria and quality assessment. Standard error of the prevalence was calculated according to binomial distribution formula. Based on the degree of heterogeneity, fixed or random effects models were applied for estimating the pooled prevalences. Results: In this study, 16 papers providing 20 evidences of CMV prevalence in Iran entered in the meta- analysis. CMV IgG and IgM seroprevalences as well as primary infection rate (95% confidence interval) among pregnant women were 92.8% (90.6 - 94.9), 6.4% (2.8 - 9.9) and 1.1% (0.7 - 1.5) respectively. CMV IgM seroprevalence among neonates were 0.6% (0.09 - 1.2), while CMV IgG and CMV IgM seroprevalences among non-pregnant women were 78.4% (70 - 86.8) and 4.6% (1.5 - 7.6) respectively. Conclusions: This meta- analysis showed that the prevalence of CMV infection among studied population is relatively high. Therefore, mortality, complications, anomalies and injuries among fetuses, neonates and immunocompromized patients can be partially related to the CMV infection.

Study of Anti-Toxoplasma IgG and IgM Seropositivity Among Subjects Referred to the Central Laboratory in Tabriz, Iran, 2013 - 2014

Background: Toxoplasmosis is one of themost widespread parasitic infections worldwide. The infection ismostly benign, although severe disease can be expected in immunocompromised/suppressed individuals and infants who are infected during pregnancy. Objectives: The aim of the present study was to determine the anti-Toxoplasma IgG and IgM seroprevalence in subjects who were referred to the Central Laboratory in Tabriz, Iran, for the diagnosis of toxoplasmosis. Methods: This retrospective cross-sectional study was carried out among 6393 individuals who were referred to the central laboratory, Tabriz University of Medical Sciences, Tabriz, Iran, from March 2013 to January 2014. The subjects were referred from different areas of East Azerbaijan province. The quantitative determination of anti-Toxoplasma IgG and IgM was performed using an antibody capture chemiluminescence immunoassay. The other available variables, including sex and age, were also recorded and analyzed. Results: In total, 1910 (29.87%) out of 6393 subjects were found to be infected with Toxoplasma gondii. In terms of the seropositivity, 1871 (32.42%) out of 5770 and 128 (2.14%) out of 5965 subjects were anti-Toxoplasma IgG and IgM seropositive, respectively. In addition, 103 (1.69%) out of 5828 subjects were equivocal with regard to anti-Toxoplasma IgM, while 57 (0.97%) out of 6068 individuals were equivocal with regard to anti-Toxoplasma IgG. The mean concentration of anti-Toxoplasma IgM was observed to be higher in male subjects than in females (P = 0.016). Conclusions: Based on the findings of the present study, the frequency of Toxoplasma infection is high in the studied population, although it is fairly close to the findings of reports from the other areas of Iran.