IgG1 Allotypes Research Articles

Allelism of genes determining two IgG1 allotypes in cattle

Animal Blood Groups and Biochemical GeneticsVolume 9, Issue 1 p. 59-63 Allelism of genes determining two IgG1 allotypes in cattle Jan Węgrzyn, Jan Węgrzyn Institute of Zootechnics, Department of Immunogenetics, 32–083 Balice near Kraków, PolandSearch for more papers by this authorZuzanna Węgrzyn, Zuzanna Węgrzyn Institute of Zootechnics, Department of Immunogenetics, 32–083 Balice near Kraków, PolandSearch for more papers by this author Jan Węgrzyn, Jan Węgrzyn Institute of Zootechnics, Department of Immunogenetics, 32–083 Balice near Kraków, PolandSearch for more papers by this authorZuzanna Węgrzyn, Zuzanna Węgrzyn Institute of Zootechnics, Department of Immunogenetics, 32–083 Balice near Kraków, PolandSearch for more papers by this author First published: January 1978 https://doi.org/10.1111/j.1365-2052.1978.tb01414.xCitations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume9, Issue1January 1978Pages 59-63 RelatedInformation

Gm Allotype Preference in Erythrocyte IgG Antibodies of Patients With Autoimmune Hemolytic Anemia

Abstract IgG antibodies were isolated from erythrocytes of five patients with autoimmune hemolytic anemia (AHA) and one case of hemolytic anemia of unknown etiology. All six persons were heterozygous for human IgG1 allotypes, and thus their serum immunoglobulins were Gm (a+f+). Quantitative analysis of the antibody preparations disclosed that three patients contained only the Gm(a) allotype; the remaining samples showed a predominance of Gm(a) in comparison to the Gm(f) allele. It was concluded that erythrocyte antibodies of patients with AHA are often restricted in respect to their Gm genetic characters with a preference for the Gm(a) allotype. In a second group of experiments, serums from three cases of hemolytic anemia were incubated with Rh+ erythrocytes from a healthy donor. Subsequently, the red cell IgG coat was eluted and analyzed. One sample had only Gm(a+) antibody; a second case showed Gm(a) predominance. The last antibody preparation varied from a disproportionately high Gm(a) concentration to equal allotype concentrations, depending on the antibody/antigen ratio used during the sensitization of the erythrocytes. The latter data suggested that allelic expression was influenced by the immunologic characteristics of the antibody subpopulation tested. No association could be demonstrated between the Ig allotypes and either the Rh specificity of the eluate, or the presence of complement coating on erythrocytes. These findings are similar to a previous report from this laboratory that noted a preference for the Gm(a) allotype in many isoantibodies with Rh specificity. The data suggest an association between certain erythrocyte antibodies and Ig allotypes.

A quantitative method for determining γG allotype antigens

The sera expression of human immunoglobulin heavy chain genes and gene complexes was analyzed in different ethnic groups by quantitative determination of Gm allotype antigens. In all populations tested, IgG3 subclass heavy chains of Gm(b+) allotype had greater sera expression than the allele Gm(g). The percentage of IgG3 allotype molecules was higher in Chinese and Easter Islanders than in Caucasians due to higher Gm(b) values. In general, IgG1 allotypes did not show quantitative differences correlated with Gm genotype. A possible exception was the Gmza, g gene complex which had a lower Gm(a) concentration than comparable Chinese sera of different genotype. Certain similarities in the regulation of Gm genes were consistently observed among the populations tested; these included the restricted range of percentage of IgG1 allotype antigens, a gene dosage effect, and differential gene activity of Gmb and Gmg IgG3 Gm genes.