This research investigates the impact of disgust towards culture mixing on acculturation and life satisfaction among mainland Chinese sojourners adapting to life in Hong Kong. In Study 1, drawing from previous research, we developed a measurement tool for assessing disgust towards mainland China-Hong Kong culture mixing and validated it in a sample of mainland Chinese students. We then conducted two longitudinal studies (Study 2 and 3) to examine the effects of disgust towards culture mixing on life satisfaction over 3-month and 6-month periods. Consistent with prior findings, our results showed that mainland Chinese sojourners perceived mixed cultural symbols as more disgusting compared to standalone ones. Cross-lagged analyses revealed that disgust towards culture mixing negatively predicted life satisfaction after 3 and 6 months of stay. Our findings indicate that initial disgust reactions towards culture mixing may have a causal influence on subsequent life satisfaction during the acculturation process. In conclusion, our study highlights the negative impact that disgust towards cultural mixing has on the acculturation process for Mainland Chinese immigrants in Hong Kong. The findings underscore the importance of promoting greater cultural awareness, understanding, and inclusiveness for the acculturative well-being of immigrants.