Conditioned pain modulation (CPM) measures the efficiency of endogenous pain inhibition and is regarded as a systemic effect. It is unknown, whether individuals respond to CPM similarly between test sites and the effect of repeated measurements. The aim of this study was to investigate within individuals 1) whether CPM magnitude is consistent between test sites within the same CPM trial, 2) whether CPM responders and non-responders are similar between test sites, and 3) if these relations change with repeated measurement (within and between sessions). Twenty subjects (19.9±1.3 years; 10 females) participated in three experimental sessions separated by approximately one week. CPM was measured 4 times; once in the first and third sessions and twice in the second session (separated by 45-minutes of quiet rest). CPM was measured as the percent change in pressure pain thresholds at the right quadriceps and deltoid muscles (test stimuli) before and during ice-water immersion (conditioning stimulus; 6±0.5°C; 2-minute duration) of the left foot. Intraclass correlations (ICCs(2,k)) were computed between assessment sites in each CPM trial. CPM responders at each assessment site were determined based on a change greater than the standard error of measurement of each assessment site. Cohen's Kappa was computed to determine the level of agreement between the sites in each CPM trial. In the first and third sessions, there was good agreement in CPM magnitude between assessment sites (ICC first session=0.611 [0.008-0.847]; ICC third session=0.535 [-0.093-0.810]) but poor agreement in responders vs. non-responders (κ first session=-0.053; κ third session=-0.098). During the second session, agreement in both CPM trials improved between the sites in terms of magnitude (ICC first trial=0.878 [0.694-0.952]; ICC second trial=0.867 [0.669-0.947]) and responders/non-responders (κ first trial=-0.762; κ second trial=-0.375). The site of the test stimulus may produce opposite outcomes and influence how endogenous pain inhibition is interpreted.