IBA Applications Research Articles

The effect of plant growth regulator bacteria on micro propagation of grapevine rootstock with three different rooting abilities

In the study, 3 American rootstocks (110 R, 1103 P and 5 BB) with low, medium, and high rooting ability were used, 2 different bacterial isolates Bacillus cereus (ZE-7) and Pseudomonas putida (ZE-12) and their binary combination and the bottom of the cuttings. It was aimed to determine the effect of bacteria on rooting by treating the rooting part. During the rooting stage, hormone-free MS medium was treated with PGPRs, and in addition, hormone-free MS medium containing 1 mgl-1 IBA was used to determine the effects of PGPRs strains. At the end of the study, the root ratio of the explants was maintained; root length, number of roots, root fresh weight, root dry weight, shoot length, shoot fresh weight, and shoot dry weight data were examined. It was determined that PGPR applications generally gave higher values than control and IBA applications. Among the rootstocks, it was determined that they gave the best results in terms of root development. The highest values were obtained in the 5BB rootstock, and the lowest values were generally obtained in the 110R rootstock. Shoot development values varied according to the rootstocks and applications. In terms of the effects of the applications on root development, the highest rooting rate was 72.03%, the highest root number ratio was 1.95 on average, and the highest root fresh weight was 39.75 mg from the Bacillus Cereus application; Additionally, the highest root dry weight was obtained from B.cereus+P.putida application with 13.06 mg. The research highlighted this feature of 5 BB rootstock, which is considered among the easily rooting rootstocks, with its PGPR effect. 110 R, which is known as a difficult rooting rootstock and has high resistance to drought and active lime, which are the biggest threats today, showed a lower rooting rate, but when the values were examined, it was above the control and IBA applications.

Differential cross–section measurements for the proton-induced alpha and deuteron reactions and elastic scattering on 9Be, suitable for Ion beam analysis applications

In the current work the differential cross sections for the proton-induced 9Be(p,d0)8Be and 9Be(p,α0)6Li nuclear reactions (Q-values = 559 and 2125 keV respectively), as well as, the 9Be(p,p0)9Be elastic scattering were measured for 6 backscattering detection angles (θ = 120°, 130°, 140°, 150°, 160° and 170°) covering the Ep,lab = 300–3000 keV energy range with an energy step between 10 and 50 keV. The total estimated uncertainties of the determined cross-section values did not exceed 6.4 % for the elastic scattering, 7.0 % for the 9Be(p,α0)6Li and 9.9 % for the 9Be(p,d0)8Be reaction. Benchmarking measurements were also performed with a suitable thick target in order to validate the obtained cross-section values. The present experimental results constitute three coherent differential cross-section datasets suitable for IBA applications. The measurements were performed at the 4 MV Tandem Dynamitron Laboratory of the Ruhr University Bochum in Germany. The experimental, data analysis and benchmarking procedures are presented in detail.

Promoting Rooting and Vegetative Growth in 41 B Vine Rootstock Cuttings with Encapsulated Nano Silver and IBA Applications

Globally, viticulture is carried on by grafting onto vine rootstocks obtained by hybridizing different vitis species or on their own roots due to their tolerance to biotic and abiotic stresses, especially phylloxera (Daktulosphaira vitifoliae). Sustainable regeneration of vineyard areas and new vineyard establishments require significant amounts of vine saplings each year. In addition to hormonal applications, nano products are also tested in the production of high quality vine nursery material. In this study, single nodal cuttings prepared from the vine rootstock 41 B (Vitis vinifera L. cv. Chasselas × Vitis berlandieri) in the resting period were used as plant material. The effects of encapsulated nano silver particles (AgNPs, 0.5 mg L-1 and 1 mg L-1), and IBA (0 and 1 g L-1) applications produced by green synthesis with cv. Öküz Gözü grape seed extract and AgNO3 on promoting rooting and vegetative growth in 41 B vine rootstock cuttings were evaluated in this study. AgNPs and IBA applications promoted rooting of cuttings, and vegetative growth of rooted cuttings. Applications increased shoot length, shoot diameter, number of nodes, fresh and dry weights of roots and shoots, and the highest value among these was achieved by the 1 mg L-1 AgNPs application. Leaf area and chlorophyll content (SPAD value) were determined by 1 g L-1 IBA application. AgNPs and IBA treatments resulted in increased rooting and vegetative growth compared to control. In conclusion, 1 mg L-1 AgNPs was recommended for vine propagation material and sapling producers as nano promotors for rooting and vegetative growth of 41 B rootstock cuttings.

Effect of Different Concentrations of IBA and Time of Taking Cutting on Propagation of Black and White Myrtle (Myrtus communis L.) Cuttings

This study aimed to evaluate the effect of different time of taking cutting and IBA concentrations on the rooting of Myrtus communis L. White and black myrtle types were used as plant materials. The cuttings were obtained from Batı Akdeniz Agricultural Research Institute (BATEM) collection garden. The effects of 0 (control), 500, 1000, 2000, 4000 and 8000 mg L-1 doses of IBA applications and time of taking cutting (February, March, April and May) on the rooting were investigated. The highest rooting percentage (76.67%) in black myrtle was obtained from cuttings taken in April and applied 1000 mg L-1 IBA, while the lowest rooting (10.00%) was obtained from cuttings applied 500 mg L-1 taken in May. White myrtle was rooted at a lower rate than black myrtle. It was determined that the highest rooting percentage (43.33%), shooting percentage (43.33%), rooted-shooted rates (43.33%) and average root number (1.63 pcs) in the white myrtle were 500 mg L-1 IBA dose in April. In addition, the study showed that the best rooting of white myrtle was in the cuttings taken in April and applied 500 mg L-1 IBA. Based on results, it can be concluded that time of taking cuttings in propagation and different concentration of IBA applications had a different impact on the success of black and white myrtle rooting.

Differential cross–section measurements and R-Matrix calculations for proton elastic scattering on natMg in the energy range Ep,lab = 2.70–4.25 MeV, suitable for EBS

In the current work the first coherent set of differential cross section values for the natMg(p,p0)natMg elastic scattering covering the Ep,lab = 2700–4250 keV energy range is presented for 6 backscattering detection angles (120°, 130°, 140°, 150°, 160° and 170°). R-Matrix calculations were implemented using the AZURE 2.0 code [1] in an attempt to reproduce the obtained experimental data, whilst taking into account the 24Mg(p,p1)24Mg reaction channel. Both results are suitable for EBS and other IBA applications and, furthermore, they form a basis for a future expansion of the current SigmaCalc [2] evaluation, once more experimental data become available. The measurements were performed in the 5.5 MV TN11 HV Tandem Accelerator and the high precision goniometer of N.C.S.R. ‘Demokritos’, Athens, Greece. The experimental and data analysis procedures are presented in detail, along with the process behind the implementation of the R-Matrix calculations.

Paclobutrazol Uygulamalarının Karadut (Morus nigra L.) Odun Çeliklerinin Köklenme Performansı Üzerine Etkileri

Cutting reproduction is the cheapest and most practical method for plants capable of clonal regeneration. However, the difficulties in adventitious root formation in black mulberry cuttings create a disadvantage for mulberry growers. This study aimed to investigate the effect of IBA and Paclobutrazol applications on the rooting ability of black mulberry hardwood cuttings. The experiment was carried out in the polyethylene greenhouse of Tokat Gaziosmanpaşa University Agricultural Research and Application Center in February 2022. As a material, hardwood cuttings prepared from one-year-old branches of breeding black mulberry trees within the body of the directorate were used. The study was set up in a randomized plot design with 3 replications and 15 cuttings in each replication. In the study, pure water as a control group, 6000 ppm IBA and doses of 1000 ppm, 2000 ppm and 3000 ppm Paclobutrazol respectively were applied to black mulberry wood cuttings separately and together. After the application, the cuttings were planted in perlite medium. The cuttings were kept in the rooting medium for 90 days and then removed; rooting rate, root number, root length and root diameter values were recorded. As a result of the study, the effect of the applications on the root number of the cuttings was not observed while the rooting rate, root length and root diameter parameters were observed. In terms of rooting rate, the best result was obtained from IBA+PBZ 1000 application (40.00%) compared to the control group (0.0%). The highest root length was determined in IBA 6000 application, and the thickest root diameter values were determined in PBZ 1000 application.

Effect of IBA, Brassinosteroid, and Bacterial Applications on Rooting of Some Rosehip (Rosa canina L.) Genotypes by Hardwood Cuttings

Rosehip is a species whose environmental and economic importance is increasing day by day. This study was carried out to determine the effect of auxin, brassinosteroid and bacteria for the adventitious rooting of hardwood cuttings belonging to the SRG 17 and SRK 26 genotypes, which were previously obtained by selection from the province of Yozgat. In the study, hardwood cuttings taken in February were used with single, double and triple IBA (2000 ppm), 24-eBL (0, 0.25, 0.50 and 1.00 ppm) and a commercial bio prepare containing bacteria species of Pseudomonas fluorescens, Paenibacillus polymyx, Bacillus megaterium and Pantoea agglomerans. After combined treatment, they were planted in perlite+peat medium and removed after three months. While the highest rooting rate of 40% in SRG17 genotype was obtained from the triple combination of Bacteria + 24-eBL (0.50 ppm) + IBA (2000 ppm), it was obtained from Bacteria application with 43.33% in SRK26 genotype. Bacteria + 24-eBL (1.00 ppm) + IBA (2000 ppm) triple combination in SRG17 genotype in terms of root length and root number provided the highest values with 8.38±4.32 (cm) and 21.00±9.00 pcs, respectively. In the SRK26 genotype, 11.18±1.41 cm root length and 36.00±12.25 pcs were obtained from the 24-eBL (0.50 ppm) + IBA (2000 ppm) binary combination. The viability rates were determined three months after the rooted cuttings were taken into the pots, and the SRG17 genotype provided 100% survival in the binary combination Bacteria + 24-eBL (0.50 ppm) and 50% in the SRK26 genotype in the 24-eBL (0.50 ppm) application.

Improving Survival and Orchard Performance of “Costata” Persimmon Seedlings by K-NAA and IBA Applications

The present investigation was conducted during the 2018 and 2019 growing seasons on one-year-old persimmon seedlings cv. “Costata” grafted on “Seedy” and “Tarabouls” rootstocks in order to improve its survival percent and orchard perfoprmance. The Seedlings were treated with K-NAA or IBA either by dipping at 3000 mg l-1 for 5 seconds or soaking at 500 mg l-1 for 24 hours. Obtained results generally showed significant increase in vegetative growth, root biomass and leaf chlorophyll and mineral content by all K-NAA and IBA applications compared to the water treated ones. A highest values of sprouting buds percent (during April, June and August), average shoots number, trunk cross-sectional area, leaf area and canopy volume were recorded with the K-NAA soaking at 500 mg l-1for 24 h. Leaf chlorophyll content was highest with K-NAA and IBA soaking in the first season, while K-NAA soaking at 500 mg l-1 for 24 h gave the highest values of leaf N, P, K, Fe, Mn and Zn in both seasons. Also, the K-NAA soaking or dipping applications recorded the highest values of average shoot length, increment percnts of shoot length and stem diameter and highest seedlings survival in both seasons. The K-NAA soaking at 500 mg l-1 for 24 h gave the highest significant values of root biomass in the first season. Results also showed that seedlings on “Seedy” rootstock surpassed the ones on “Tarabouls” rootstock in all vegetative growth parameters and root biomass, but contained lower leaf N, P, Mn and Zn.

Effects of Bacteria and Iba on the Rooting of Citrange Citrus Rootstocks Cuttings

In this study, the effects of Agrobacterium rubi A-18 and Bacillus OSU-142 bacterial strains alone and in combination with 1000, 2000, 4000 ppm IBA on rooting of Carrizo citrange and Troyer citrange citrus rootstock in softwood, semi-hardwood and hardwood cuttings were investigated. In the case of IBA solution, Agrobacterium rubi A-18 and Bacillus OSU-142 were prepared in solution at a concentration of 1x109 bacteria / ml and applied to cuttings of citrus. Application were performed in the mist propagation system. Cuttings kept in the fogging environment for 3 months removed at the end of this period, and their rooting rates (%), callus formation rates (%) and survival rates (%) determined. Based on the results of the study, the survival rates and callus formation rates of the cuttings of citrus were generally high at the end of the rooting period. The highest rooting rates in Carrizo citrange cuttings were detected in 4000 ppm IBA and OSU-142 + A-18 (13.33%) treatments for softwood cuttings, 4000 ppm IBA + OSU-142 (20.00%) in semi-hardwood and hardwood cuttings. In the Troyer citrange, OSU-142 + A-18 (6.67%) treatments for softwood cuttings, in 1000 ppm IBA for semi-hardwood, and in 4000 ppm IBA (13.33%) and 1000 ppm IBA treatment for hardwood cuttings, 2000 ppm IBA and OSU-142 (13.33%) the highest rooting has been treatments. According to the cuttings pick-up period, the rooting rates of Carrizo citrange are not different, at the Troyer citrange in the semi-hardwood and hardwood cuttings was higher. As a result, it could be state that plant growth promoting bacteria and IBA applications have not effect on rooting in the softwood, semi-hardwood and hardwood cuttings of the Carrizo citrange and Troyer citrange citrus rootstocks.

Automating the setup and control of the pre-sample charge measurement system at the Lund Ion-beam Analysis Facility

In many IBA applications the aim is to obtain quantitative figures characterising the sample, usually requiring a reliable measurement of the beam current for normalisation. To avoid the drawbacks associated with on-sample and post-sample charge measurement systems a pre-sample measurement system can be used.In this work an upgrade to the pre-sample charge measurement system at the Lund Ion-beam Analysis Facility is presented, in which a custom built control module based around a Teensy 3.2 micro-controller has been added to the existing system. The new control module allows for remote handling and monitoring of the deflection system, operated via USB connection to a PC running a graphical user interface. Details of the control modules functionality, design and programming are given, as well as the functionality of the user interface. An example of how the new control module can be used for automated calibration of the deflection system is also presented.

Effects of Bacteria and IBA on the Rooting of Bitter Orange (Citrus aurantium L.) and Trifoliate Orange (Poncirus trifoliata Raf.) Cuttings

In this study, the effects of Agrobacterium rubi A-18 and Bacillus OSU-142 bacterial strains alone and in combination with 1000, 2000, 4000 ppm IBA on rooting of bitter orange and trifoliate orange citrus rootstock in softwood, semi-hardwood and hardwood cuttings were investigated. In the case of IBA solution, Agrobacterium rubi A-18 and Bacillus OSU-142 were prepared in solution at a concentration of 1x10 9 bacteria / ml and applied to steel. Application is made in the mist propagation system, in the perlite are planted in the environment. Steels kept in the fogging environment for 3 months were removed at the end of this period and rooting rates (%), callus formation rates (%) and survival rates (%) were determined. According to the results of the research, the rooting rates of the steels taken at different periods were found to be low. The highest rooting rates of bitter orange softwood cuttings were 2000 ppm IBA + OSU-142 (6.67%), semi-hardwood cuttings OSU-142 (20.00%), and 4000 ppm IBA + OSU-142 (20.00%) in hardwood cuttings. It has been not rooting in trifoliate orange softwood cuttings, the highest rooting was in the case of semi-hardwood cuttings with 4000 ppm IBA + OSU-142 (26.67%), in hardwood cuttings 1000 ppm IBA + OSU-142, 2000 ppm IBA + OSU-142, 4000 ppm IBA + OSU-142, 1000 ppm IBA + A-18, 2000 ppm IBA + A-18 and 4000 ppm IBA + A-18 (20.00%). According to the cuttings pick-up period, the rooting rates of bitter orange are not different between the periods, in the trifoliate orange the hardwood cuttings were found to be higher. As a result, it can be said that plant growth promoting bacteria and IBA applications have no effect on rooting in the softwood, semi-hardwood and hardwood cuttings of the bitter orange and trifoliate orange citrus rootstocks, it can be said that studies on rooting in these rootstocks can be useful in different bacterial breeds

Statistical comparison of leaching behavior of incineration bottom ash using seawater and deionized water: Significant findings based on several leaching methods

Bottom ashes generated from municipal solid waste incineration have gained increasing popularity as alternative construction materials, however, they contains elevated heavy metals posing a challenge for its free usage. Different leaching methods are developed to quantify leaching potential of incineration bottom ashes meanwhile guide its environmentally friendly application. Yet, there are diverse IBA applications while the in situ environment is always complicated, challenging its legislation. In this study, leaching tests were conveyed using batch and column leaching methods with seawater as opposed to deionized water, to unveil the metal leaching potential of IBA subjected to salty environment, which is commonly encountered when using IBA in land reclamation yet not well understood. Statistical analysis for different leaching methods suggested disparate performance between seawater and deionized water primarily ascribed to ionic strength. Impacts of leachant are metal-specific dependent on leaching methods and have a function of intrinsic characteristics of incineration bottom ashes. Leaching performances were further compared on additional perspectives, e.g. leaching approach and liquid to solid ratio, indicating sophisticated leaching potentials dominated by combined geochemistry. It is necessary to develop application-oriented leaching methods with corresponding leaching criteria to preclude discriminations between different applications, e.g., terrestrial applications vs. land reclamation.

Development of a Reference Database for Particle-Induced Gamma-ray Emission spectroscopy

Particle-Induced Gamma-ray Emission (PIGE) is a powerful analytical technique that exploits the interactions of rapid charged particles with nuclei located near a sample surface to determine the composition and structure of the surface regions of solids by measurement of characteristic prompt γ rays. The potential for depth profiling of this technique has long been recognized, however, the implementation has been limited owing to insufficient knowledge of the physical data and lack of suitable user-friendly computer codes for the applications. Although a considerable body of published data exists in the nuclear physics literature for nuclear reaction cross sections with γ rays in the exit channel, there is no up-to-date, comprehensive compilation specifically dedicated to IBA applications. A number of PIGE cross-section data had already been uploaded to the Ion Beam Analysis Nuclear Data Library (IBANDL) (http://www-nds.iaea.org/ibandl) by members of the IBA community by 2011, however a preliminary survey of this body of unevaluated experimental data has revealed numerous discrepancies beyond the uncertainty limits reported by the authors. Using the resources and coordination provided by the IAEA, a concerted effort to improve the situation was made within the Coordinated Research Project on the Development of a Reference Database for PIGE spectroscopy, from 2011 to 2015. The aim of the CRP was to create a data library for Ion Beam Analysis that contains reliable and usable data on charged particle γ-ray emission cross sections that would be made freely available to the user community. As the CRP has reached its completion, we shall present its main achievements, including the results of nuclear cross-section evaluations and the development of a computer code that will become available to the public allowing for the implementation of a standardless PIGE technique.

A simple Bragg detector design for AMS and IBA applications

A new compact Bragg type gas ionization chamber (GIC) has been built for use as particle counter in AMS and IBA applications. The detector stands out due to its simple concept, which does not include a Frisch grid. Test experiments have been performed with ions in the mass range from He to Th and energies ranging from 30keV to 2.5MeV, in order to find optimal measurement conditions and to characterize the detector performance. For projectiles heavier than Al at energies below 2.5MeV the obtained energy resolution is comparable with that of a state-of-the-art GIC with Frisch grid and clearly outperforms solid state detectors. Additionally the operation of this simplified Bragg GIC in the electron multiplication mode was investigated for the first time, which allows the detection of radiocarbon ions at energies below 50keV with an energy resolution of the order of 10keV.

Stopping power of palladium for protons in the energy range 0.300–3.100 MeV

The stopping power of palladium for protons has been measured using the transmission method with an overall uncertainty of around 5% over the energy range Ep=(0.300–3.100)MeV. These stopping power data are then compared to stopping power values calculated by the SRIM-2010 code and to those derived from a model based on the dielectric formalism. Subsequently, and within the framework of the modified Bethe–Bloch theory, this stopping power data were used for extracting Pd target mean excitation and ionization potential, (I=468±5eV), and Barkas effect parameter, (b=1.51±0.06). A good agreement is found between the obtained results and values reported in literature.It is worth mentioning that these are the first reported results for protons on palladium over this energy range, which is often used in IBA applications, such as Rutherford Backscattering Spectrometry (RBS) and Proton Induced X-ray Emission (PIXE).

A pre-sample charge measurement system for quantitative NMP-analysis

In many IBA applications the main aim is to obtain quantitative figures characterizing the sample. Normally charge, i.e. number of probe particles, is used for normalization and is measured either by collecting the charge deposited in the sample or by collecting the particle in a post-sample Faraday cup or in combination. Both these techniques have drawbacks and results can be difficult to compare for samples with different matrix composition. In this work, we present an upgraded design and test results from the Lund NMP pre-sample charge measurement system. The system presented is based on a pre-sample beam deflection controlled by the beam scanning system for the nuclear microprobe. It can be operated in different modes, but during normal operation the beam is blanked once per pixel and the corresponding charge is collected during the beam-off period. The system does not only measure an average of the beam current during data collection, but actually a pixel-by-pixel normalization is possible. Data of the system performance are presented and in addition illustrations of how quantitative measurements both for PIXE and elastic scattering can be made more reliable. (C) 2010 Elsevier B.V. All rights reserved. (Less)

Clonal propagation through air layering in four endemic trees of Western Ghats

The endemic plants of the Western Ghats of Peninsular India are facing severe threat of endangerment due to various physical and biological reasons. Many of these plants especially the trees are lacking any kind of propagation and multiplication strategies and are under brink of depletion even without keeping an alternate genetic stock. In this context, as part of the study on the development of propagation profiles and restoration of economically important endemic plants of the Western Ghats, four economically important endemic trees, viz., Antidesma montanum Blume, Humboldtia vahliana Wt., Lagerstroemia macrocarpa Wt. and Syzygium mundagom (Bourd.) Chithra, were investigated for their clonal rooting ability. Ring air layering on the lateral branches of the young stands of 3-5 year age was achieved with 50-60% success for the first time on NAA and IBA applications at 1000-1500 ppm, during the months of August-September. The success of air layering thus is recommended as a low cost and viable propagation method for multiplication and subsequent restoration and conservation, which in turn ensure the availability of materials for resource base utilization without affecting natural populations of the species in the long run.

The novel HVEE 5 MV Tandetron™

Recently, HVEE has extended the voltage range for its Tandetron™ accelerators from 3 MV terminal voltage to 5 MV terminal voltage with the development of an entirely new coaxial Tandetron™. The new 5 MV system is presently in the final test phase and will shortly be installed at the Universidad Autonoma de Madrid (Spain) as part of their new IBA facility. The all-solid-state power supply (parallel-fed Cockroft–Walton type) is constructed around the high-energy accelerator tubes, thereby avoiding the T-shaped tank that was so far characteristic for the HVEE Tandetrons™. During the design of the system special emphasis has been put to minimize the electrical field strength in the complete structure. Using three-dimensional electrostatic field simulations, we were able to identify possible hot spots and to reduce the maximum field strength to 80% compared to that of older designs. This reduction in field strength guarantees more reliable operation at or even above the guaranteed terminal voltage of 5 MV. The electrical power for beam transport is generated by a 10 kW version of a recently in-house developed range of all-solid-state drivers with output powers of up to 25 kW. Apart from IBA applications like heavy element ERDA and NRA, the system is very well suited for other applications like positron emission tomography, deep implants in semiconductors as well as accelerator mass spectrometry of various elements, including 36 Cl and 41 Ca .

The pulsed beam facility at the 3 MV Van de Graaff accelerator in Florence: Overview and examples of applications

An electrostatic chopper has been installed at the KN 3000 accelerator in Florence to obtain short beam pulses with a number of particles per pulse whose average value can be chosen by varying the current intensity at the deflector plates input. Beam pulses can be obtained containing an average number of particles per pulse from less than one to thousands. The transmitted beam pulses can be as short as 200 ps FWHM, at a repetition rate up to about 100 kHz. Among the many applications of the facility, the direct measurement of energy loss and straggling of protons in Kapton and aluminium is reported. In this measurement, the facility has been tuned for transmission of mainly single-proton pulses; the beam energy is directly measured downstream with a good energy-resolution detector, without and with absorbers in front. In general, measurements of this kind can be directed both to study the basic processes of charged particles interactions in materials, or more practically to obtain the effective values of energy parameters useful in many IBA applications, avoiding the need to rely on simulations or theoretical estimates. Also briefly described is an application to Si-detector testing. In this case, the facility has been tuned for transmission of pulses containing many hundreds of protons of energy E p=2.5 MeV and the detector is directly exposed to the pulses. Spectra containing equally spaced peaks at energies multiple of E p are obtained and the response linearity of the detector plus electronics system can thus be checked.

APPLICATIONS OF ION BEAM ANALYSIS TO THE ELEMENTAL AND ISOTOPIC CHARACTERISATION OF ADVANCED MATERIALS

The accelerator laboratory of the Groupe de Physique des Solides, Paris, has pioneered a number of IBA techniques and applications over the last few decades. In particular, in the 1960's and 1970's, Nuclear Reaction analysis (NRA) including nuclear resonance depth profiling, isotopic tracing combined with NRA, and channelling techniques were developed under the leadership of G. Amsel. In this paper I will present a selection of recent applications from this laboratory of NRA, isotopic tracing with stable isotopes, and ion channelling, intended to illustrate the present status of these techniques in condensed matter physics and materials science, and to act as a backdrop for a discussion of future directions for development of Ion Beam Analysis in condensed matter physics and advanced materials research.