Effects of Bacteria and IBA on the Rooting of Bitter Orange (Citrus aurantium L.) and Trifoliate Orange (Poncirus trifoliata Raf.) Cuttings

Abstract

In this study, the effects of Agrobacterium rubi A-18 and Bacillus OSU-142 bacterial strains alone and in combination with 1000, 2000, 4000 ppm IBA on rooting of bitter orange and trifoliate orange citrus rootstock in softwood, semi-hardwood and hardwood cuttings were investigated. In the case of IBA solution, Agrobacterium rubi A-18 and Bacillus OSU-142 were prepared in solution at a concentration of 1x10 9 bacteria / ml and applied to steel. Application is made in the mist propagation system, in the perlite are planted in the environment. Steels kept in the fogging environment for 3 months were removed at the end of this period and rooting rates (%), callus formation rates (%) and survival rates (%) were determined. According to the results of the research, the rooting rates of the steels taken at different periods were found to be low. The highest rooting rates of bitter orange softwood cuttings were 2000 ppm IBA + OSU-142 (6.67%), semi-hardwood cuttings OSU-142 (20.00%), and 4000 ppm IBA + OSU-142 (20.00%) in hardwood cuttings. It has been not rooting in trifoliate orange softwood cuttings, the highest rooting was in the case of semi-hardwood cuttings with 4000 ppm IBA + OSU-142 (26.67%), in hardwood cuttings 1000 ppm IBA + OSU-142, 2000 ppm IBA + OSU-142, 4000 ppm IBA + OSU-142, 1000 ppm IBA + A-18, 2000 ppm IBA + A-18 and 4000 ppm IBA + A-18 (20.00%). According to the cuttings pick-up period, the rooting rates of bitter orange are not different between the periods, in the trifoliate orange the hardwood cuttings were found to be higher. As a result, it can be said that plant growth promoting bacteria and IBA applications have no effect on rooting in the softwood, semi-hardwood and hardwood cuttings of the bitter orange and trifoliate orange citrus rootstocks, it can be said that studies on rooting in these rootstocks can be useful in different bacterial breeds