Effects of Bacteria and Iba on the Rooting of Citrange Citrus Rootstocks Cuttings

Abstract

In this study, the effects of Agrobacterium rubi A-18 and Bacillus OSU-142 bacterial strains alone and in combination with 1000, 2000, 4000 ppm IBA on rooting of Carrizo citrange and Troyer citrange citrus rootstock in softwood, semi-hardwood and hardwood cuttings were investigated. In the case of IBA solution, Agrobacterium rubi A-18 and Bacillus OSU-142 were prepared in solution at a concentration of 1x109 bacteria / ml and applied to cuttings of citrus. Application were performed in the mist propagation system. Cuttings kept in the fogging environment for 3 months removed at the end of this period, and their rooting rates (%), callus formation rates (%) and survival rates (%) determined. Based on the results of the study, the survival rates and callus formation rates of the cuttings of citrus were generally high at the end of the rooting period. The highest rooting rates in Carrizo citrange cuttings were detected in 4000 ppm IBA and OSU-142 + A-18 (13.33%) treatments for softwood cuttings, 4000 ppm IBA + OSU-142 (20.00%) in semi-hardwood and hardwood cuttings. In the Troyer citrange, OSU-142 + A-18 (6.67%) treatments for softwood cuttings, in 1000 ppm IBA for semi-hardwood, and in 4000 ppm IBA (13.33%) and 1000 ppm IBA treatment for hardwood cuttings, 2000 ppm IBA and OSU-142 (13.33%) the highest rooting has been treatments. According to the cuttings pick-up period, the rooting rates of Carrizo citrange are not different, at the Troyer citrange in the semi-hardwood and hardwood cuttings was higher. As a result, it could be state that plant growth promoting bacteria and IBA applications have not effect on rooting in the softwood, semi-hardwood and hardwood cuttings of the Carrizo citrange and Troyer citrange citrus rootstocks.