Hz Frequency Research Articles

Exploring the AC conductivity and dielectric properties of PVA films doped with Erythrosin B

Erythrosin B (EB) doped in PVA film was processed utilizing the casting procedure, and the structure was investigated via the Fourier Transformation infrared technique (FTIR). FTIR analysis presented a strong formation of intermolecular hydrogen bonds among PVA and EB hydroxyl groups (OH). Characteristic temperature and frequency variation of dielectric behavior and AC electrical conductivity for films of PVA doped with Erythrosin B were analyzed at (293–383 K) temperature and (102–106 Hz) frequency. The AC conductivity dependency indicates that the predominant conduction mechanism in PVA doped with Erythrosin B films follows the correlated barrier hopping (CBH) model. The charge carriers’ hopping involving localized states causes a relaxation process. Estimates were made for the AC conductivity activation energy and the conductivity relaxation energy. Frequency and temperature affect the dielectric constant (ε′) and the dielectric loss (ε″). The complex impedance spectrum (Z′ versus Z″) showed single semicircular arcs with a decreased radius with increasing temperature. The relaxation peaks in the electric modulus representation are clearly defined, and the relaxation period has been calculated.

Fatigue-related changes in intermuscular electromyographic coherence across rotator cuff and deltoid muscles in individuals with and without subacromial pain.

Neuromuscular fatigue induces superior migration of the humeral head in individuals with subacromial pain. This has been attributed to weakness of rotator cuff muscles and overactive deltoid muscles. Investigation of common inputs to motoneuron pools of the rotator cuff and deltoid muscles offers valuable insight into the underlying mechanisms of neuromuscular control deficits associated with subacromial pain. This study aims to investigate intermuscular coherence across the rotator cuff and deltoid muscles during a sustained submaximal isometric fatiguing contraction in individuals with and without subacromial pain. Twenty symptomatic and 18 asymptomatic young adults participated in this study. Surface electromyogram (EMG) was recorded from the middle deltoid (MD) and infraspinatus (IS). Intramuscular EMG was recorded with fine-wire electrodes in the supraspinatus (SS). Participants performed an isometric fatiguing contraction of 30° scaption at 25% maximum voluntary contraction (MVC) until endurance limit. Pooled coherence of muscle pairs (SS-IS, SS-MD, IS-MD) in the 2-5 Hz (delta), 5-15 Hz (alpha), and 15-35 Hz (beta) frequency bands during the initial and final 30 s of the fatigue task were compared. SS-IS and SS-MD delta-band coherence increased with fatigue in the asymptomatic group but not the symptomatic group. In the alpha and beta bands, SS-IS and SS-MD coherence increased with fatigue in both groups. IS-MD beta-band coherence was greater in the symptomatic than the asymptomatic group. Individuals with subacromial pain failed to increase common drive across rotator cuff and deltoid muscles and have altered control strategies during neuromuscular fatigue. This may contribute to glenohumeral joint instability and subacromial pain experienced by these individuals.NEW & NOTEWORTHY Through the computation of shared neural drive across glenohumeral muscles, this study reveals that individuals with subacromial pain were unable to increase shared neural drive within the rotator cuff and across the supraspinatus and deltoid muscles during neuromuscular fatigue induced by sustained isometric contraction. These deficits in common drive across the shoulder muscles likely contribute to the joint instability and pain experienced by these individuals.

Impact of high (1950 MHz) and extremely low (50 Hz) frequency electromagnetic fields on DNA damage caused by occupationally relevant exposures in human derived cell lines

Epidemiological studies indicate that electromagnetic fields (EMF) are associated with cancer in humans. Exposure to mobile phone specific high frequency fields (HF-EMF) may lead to increased glioma risks, while low frequency radiation (LF-EMF) is associated with childhood leukemia. We studied the impact of HF-EMF (1950 MHz, UMTS signal) on DNA stability in an astrocytoma cell line (1321N1), and the effect of LF-EMF (50 Hz) in human derived lymphoma (Jurkat) cells. To find out if these fields affect chemically induced DNA damage, co-exposure experiments were performed. The cells were exposed to HF-EMF or LF-EMF and treated simultaneously and sequentially with mutagens. The compounds cause DNA damage via different molecular mechanisms, i.e. pyrimidine dimers which are characteristic for UV light (4-nitroquinoline 1-oxide, 4NQO), bulky base adducts (benzo[a]pyrene diolepoxide, BPDE), DNA-DNA and DNA-protein cross links and oxidative damage (NiCl2, CrO3). DNA damage was measured in single cell gel electrophoresis (comet) assays. We found a moderate reduction of basal and 4NQO-induced DNA damage in the astrocytoma line, but no significant alterations of chemically induced DNA migration by the HF and LF fields under all other experimental series. The biological consequences of the moderate reduction remain unclear, but our findings indicate that acute mobile phone and power line specific EMF exposures do not enhance genotoxic effects caused by occupationally relevant chemical exposures.

Pulsed-field ablation: an alternative ablative method for gastric electrophysiological intervention.

Pulsed-field ablation (PFA) is an emerging ablative technology that has been used successfully to eliminate cardiac arrhythmias. As a nonthermal technique, it has significant benefits over traditional radiofrequency ablation with improved target tissue specificity and reduced risk of adverse events during cardiac applications. We investigated whether PFA is safe for use in the stomach and whether it could modulate gastric slow waves. Female weaner pigs were fasted overnight before anesthesia was induced using tiletamine hydrochloride (50 mg·mL-1) and zolazepam hydrochloride (50 mg·mL-1) and maintained with propofol (Diprivan 2%, 0.2-0.4 mg·kg-1·min-1). Pulsed-field ablation was performed on their gastric serosa in vivo. Adjacent point lesions (n = 2-4) were used to create a linear injury using bipolar pulsed-field ablation consisting of 40 pulses (10 Hz frequency, 0.1 ms pulse width, 1,000 V amplitude). High-resolution electrical mapping defined baseline and postablation gastric slow-wave patterns. A validated five-point scale was used to evaluate tissue damage in hematoxylin and eosin-stained images. Results indicated that PFA successfully induced complete conduction blocks in all cases, with lesions through the entire thickness of the gastric muscle layers. Consistent postablation slow-wave patterns emerged immediately following ablation and persisted over the study period. Pulsed-field ablation induces rapid conduction blocks as a tool to modulate slow-wave patterns, indicating it may be suitable as an alternative to radiofrequency ablation.NEW & NOTEWORTHY Results show that pulsed-field ablation can serve as a gastric slow-wave intervention by preventing slow-wave propagation across the lesion site. Stable conduction blocks were established immediately following energy delivery, faster than previous examples of radiofrequency gastric ablation. Pulsed-field ablation may be an alternative for gastric slow-wave intervention, and further functional and posthealing studies are now warranted.

Delta waves as a sign of cortical plasticity after full-face transplantation

This study focused on detecting the reflections of healing and change in cortex activation in full-face transplantation and lesions patients on EEG activity. Face transplant patients have facial lesions before transplantation and, to identify pre-face transplant patients' brain activity in the absence of pre-transplant recordings, we used data obtained from pre-transplant facial lesion patients. Ten healthy, four facial lesion and three full-face transplant patients participated in this study. EEG data recorded for four different sensory stimuli (brush from the right face, right hand, left face, and left-hand regions) were analyzed using wavelet packet transform method. EEG waves were analyzed for standard bands. Our findings indicate significant change in the 2–4 Hz frequency range which may be a result of ongoing or previous cortical reorganization for face lesion and transplant patients. Alterations of the delta wave seen in patients with facial lesion and face transplant can also be explained by the intense central plasticity. Our findings show that the delta band differences might be used as a marker in the evaluation of post-transplant cortical plasticity in the future.

How P‐Wave Scattering Throughout the Entire Mantle Mimics the High‐Frequency Pdiff and Its Coda

AbstractWe document the arrival of seismic energy in the core shadow zone up to large distances beyond 150° more than 100 s prior to the core phases. Numerical simulations of the energy transport in an established heterogeneity model show that scattering throughout the entire mantle explains these observations. Diffraction at the core‐mantle boundary is unlikely in our 1–2 Hz frequency band and is not required indicating misleading terminology with reference to Pdiff for the scattered P∗P‐energy. Records of the largest deep earthquakes at low‐noise stations are key to the observation of the faint precursory signal which changes appearance with increasing distance from a coda‐like decay over a constant amplitude level around 130° to an emergent wave train. According to our simulations, different depth layers in the mantle dominate different time‐distance windows of the scattered wave train, providing the opportunity to improve the depth resolution of mantle heterogeneity models.

Sound absorption uncertainty in small reverberation chambers

Manufacturers in the mobility industry typically measure and specify the sound absorption coefficient of materials using a small reverberation chamber according to SAE J2883. These chambers have a 6 to 25 cubic meter volume, use a 1.0 meter x 1.2 meter sample size and report sound absorption in the one-third octave bands from about 400 Hz to 10,000 Hz. This is in contrast to a full-size chamber built to satisfy the requirements of ASTM C423 or ISO 354, which have about a 200 cubic meter volume, use a 2.4 x 2.7 or 3.2 x 3.2 square meter sample size and report sound absorption in one-third octave bands from 100 to 5,000 Hz. Although the room and sample size dimensions scale, many of the factors that contribute to uncertainty in the measurement do not scale. These include relative humidity, temperature, and other physical measurements and if the same instrumentation is used, microphone calibration and measurement uncertainty in the higher frequency range. This paper examines the potential measurement uncertainty in these chambers over the 400 to 10,000 Hz frequency range and compares this to past uncertainty analyses of full-size chambers over a similar frequency range.

Effect of Synthetic Jet on Flow Field over Different Shaped Ribs and Its Impact on Heat Transfer

The present work reports the manipulation of the flow field over different shapes of surface-mounted obstacles using a synthetic jet. A round synthetic jet is placed upstream and downstream of surface-mounted ribs on a flat surface. Particle image velocimetry and hotwire anemometry are used to study the flow field characteristics and features. Heat transfer rate was obtained using transient liquid crystal thermography technique. Experiments are carried out at Reynolds number of 32,000 in a low-speed wind tunnel. Flow field features behind ribs are analyzed using time-averaged velocity, streamline, vorticity Reynolds stress and turbulent intensity. The spanwise average Nusselt number distribution for the downstream rib location is compared for two optimum locations of the synthetic jet relative to the rib. The recirculation bubble length formed downstream of a rib varies with the frequency of the synthetic jet, its location, and the shape of the rib. A drastic reduction in reattachment length is observed at 50 and 80 Hz frequencies for square rib. The highest heat transfer rate is obtained for the square rib at 50 Hz and 4 voltage peak-to-peak excitation amplitude for the upstream location of the synthetic jet relative to the rib.

Self-replenishing lubrication mechanism of nano-silver-reinforced AISI 4140 steel composites under dry sliding for mechanical applications

Wear is one of the most important factors limiting mechanical components' lifespan and durability. In this study, a novel, AISI 4140-Ag steel-based, self-lubricating nanocomposite was manufactured by the spark plasma sintering (SPS) technique. The high-level aim of this investigation is to obtain a deeper understanding of how silver (Ag) at the nanoscale can enhance the self-lubricating layer's replenishment, which aids in maintaining a steady supply of solid lubricant under dry conditions. Tribological experiments were conducted under various loads (30–150 N) at 5 Hz frequency and 300 °C temperature using a Rtec Universal Tribometer (ball-on-disc setup). After that, advanced surface characterization techniques were utilized to illustrate predominant wear modes, lubrication mechanisms, and the chemical identity of the self-lubrication layer. Noticeably, the AISI 4140-Ag nanocomposite reduced the friction coefficient and wear rate by 36.16–51.14 % and 93.40–97.36 %, respectively, compared to the AISI 4140 composite. The excellent self-lubricating performance is mainly attributed to the replenishment of a self-lubricating layer on the substrate material and a transfer layer on the counterbody, composed of AgO and Fe2O3. The results of our study hold considerable promise for the advancement of self-lubricating composites and the enhancement of their durability in mechanical applications.

Beta-frequency sensory stimulation enhances gait rhythmicity through strengthened coupling between striatal networks and stepping movement.

Stepping movement is delta (1-4 Hz) rhythmic and depends on sensory inputs. In addition to delta rhythms, beta (10-30 Hz) frequency dynamics are also prominent in the motor circuits and are coupled to neuronal delta rhythms both at the network and the cellular levels. Since beta rhythms are broadly supported by cortical and subcortical sensorimotor circuits, we explore how beta-frequency sensory stimulation influences delta-rhythmic stepping movement, and dorsal striatal circuit regulation of stepping. We delivered audiovisual stimulation at 10 Hz or 145 Hz to mice voluntarily locomoting, while simultaneously recording stepping movement, striatal cellular calcium dynamics and local field potentials (LFPs). We found that 10 Hz, but not 145 Hz stimulation prominently entrained striatal LFPs. Even though sensory stimulation at both frequencies promoted locomotion and desynchronized striatal network, only 10 Hz stimulation enhanced the delta rhythmicity of stepping movement and strengthened the coupling between stepping and striatal LFP delta and beta oscillations. These results demonstrate that higher frequency sensory stimulation can modulate lower frequency dorsal striatal neural dynamics and improve stepping rhythmicity, highlighting the translational potential of non-invasive beta-frequency sensory stimulation for improving gait.

Electrical, Optical and Thermal Properties of Ge-Si-Sn-O Thin Films.

This work evaluates the electrical, optical and thermal properties of Sn-doped GexSi1-xOy thin films for use as microbolometer sensing materials. The films were prepared using a combination of a radio frequency (RF) magnetron and direct current (DC) sputtering using a Kurt J Leskar Proline PVD-75 series sputtering machine. Thin films were deposited in an O2+Ar environment at a chamber pressure of 4 mTorr. The thicknesses of the thin films were varied between 300 nm-1.2 µm by varying the deposition time. The morphology and microstructure of thin films were investigated by atomic force microscope (AFM) imaging and X-ray diffraction (XRD), while the atomic composition was determined using the energy dispersive spectroscopy (EDS) function of a scanning electron microscope. The thin film with an atomic composition of Ge0.45Si0.05Sn0.15O0.35 was found to be amorphous. We used the Arrhenius relationship to determine the activation energy as well as temperature coefficient of resistance of the thin films, which were found to be 0.2529 eV and -3.26%/K, respectively. The noise voltage power spectral density (PSD) of the film was analyzed using a Primarius-9812DX noise analyzer using frequencies ranging from 2 Hz to 10 kHz. The noise voltage PSD of the film was found to be 1.76 × 10-11 V2/Hz and 2.78 × 10-14 V2/Hz at 2 Hz and 1KHz frequencies, respectively. The optical constants were determined using the ellipsometry reflection data of samples using an RC2 and infrared (IR) VASE Mark-II ellipsometer from J A Woollam. Absorption, transmission and reflection data for a wavelength range of 900 nm-5000 nm were also determined. We also determined the optical constant values such as the real and imaginary parts of refractive index (n and k, respectively) and real and imaginary part of permittivity (ε1 and ε2, respectively) for wavelength ranges between 193 nm to 35 µm. An optical band gap of 1.03 eV was determined from absorption data and using Tauc's equation. In addition, the thermal conductivity of the film was analyzed using a Linseis thin film analyzer employing the 3ω method. The thermal conductivity of a 780 nm thick film was found to be 0.38 Wm-1K-1 at 300 K. From the data, the Ge-Si-Sn-O alloy was found to be a promising material for use as a sensing material for microbolometers.

Efficiently high energy storage density in Ba2+ ion modified K0·5Na0·5NbO3 (KNN) ferroelectric ceramics for super capacitors

In the present work, Perovskites BaxK0.5-xNa0.5NbO3 (x = 0.00, 0.05, 0.10, 0.15, 0.20) were synthesized via solid-state synthesis technique. XRD analysis confirmed the successful phase formation of the synthesized perovskites. Further, Rietveld refinement revealed the presence of coexisting orthorhombic and tetragonal phases. High-resolution transmission electron microscopy (HRTEM) was employed to explore the lattice fringes and SAED patterns at nano scale level of prepared samples. The morphology was investigated using a field emission scanning electron microscope (FESEM). Dielectric measurements indicated that both the dielectric constant (ε′) and loss tangent (tan δ) exhibited significant dispersion at low frequencies and high temperatures. Notably, the Ba-doped perovskite samples demonstrated enhanced dielectric properties compared to the undoped KNN sample, with Ba0·1K0·4Na0·5NbO3 (x = 0.10) showing optimal dielectric performance, characterized by a dielectric constant (ɛ՛⁓4000) and a tanẟ ⁓7.23 at 100 Hz frequency and room temperature.AC conductivity is observed to increase with increasing both frequency and temperature. DC conductivity exhibited positive temperature coefficient indicating the semiconductor-like behavior of the prepared samples. The ferroelectric behavior was confirmed through P-E hysteresis curves and Ba0·1K0·4Na0·5NbO3 sample revealed the highest energy storage density among all prepared samples making it a suitable candidate for energy storage applications.

Novel Phononic-Like Crystal Wave Barrier Structure for Vibration Isolation

As an efficient, environmentally friendly, and economical urban public transportation tool, the subway has significant advantages compared to other urban public transportation tools and plays an irreplaceable role in the urban rail transit system. While the subway brings many conveniences to people, its operation has also brought a series of problems to urban development and residents’ lives, especially the vibration and noise problems generated by the subway have become unavoidable and urgent problems to be solved. The traditional wave barriers have a certain effect on attenuating and suppressing the vibration generated by the subway, but it has shortcomings in terms of the width and quantity of elastic wave bandgap (BG), so its application in low-frequency vibration reduction and isolation of subway systems is greatly limited. In order to broaden the elastic BG width and quantity of the wave barrier, this paper designs a novel phononic-like crystal subway wave barrier (PLCSWB) for subway vibration isolation based on locally resonant theory. Firstly, the dispersion curves of the novel PLCSWB are calculated using the improved plane wave expansion method and finite element method (FEM), and compared with the dispersion curves of traditional phononic crystal (PC) wave barriers. Secondly, the frequency response function of the novel PLCSWB is calculated using FEM to evaluate its attenuation effect on vibration, and the energy distribution characteristics at its BG boundary are analyzed. Then, the main factors affecting the BG of the novel PLCSWB are analyzed, and a spring–mass system equivalent model of the novel PLCSWB is established to theoretically estimate its BG range. Finally, the vibration data of the tunnel wall monitored on site are used to analyze and verify the vibration isolation effect of the novel PLCSWB. The results show that the novel PLCSWB opens five low-frequency BGs in the 200[Formula: see text]Hz frequency band, which increased the total width of the opened BGs by 49% compared to traditional PC wave barriers. In the frequency range of the BG, the attenuation value of the novel PLCSWB to vibration is mostly above 30[Formula: see text]dB, and the energy distribution inside the structure is mainly concentrated in the primitive cell that controls the BG, indicating good attenuation and control effects on vibration. The main factors affecting the BG of the novel PLCSWB are the density of the scatterer material, the elastic modulus, and the thickness of the wrapping layer material. Moreover, during subway operation, the novel PLCSWB has a good attenuation effect on the vibration transmitted from the tunnel wall to the soil. Among them, the maximum vibration acceleration amplitude of the novel PLCSWB with nine rows is reduced by 47%, and the vibration isolation performance is remarkable. The relevant research results of this paper provide a novel approach and method for the study of subway vibration reduction and isolation, and can also provide specific references for the design and development of wave barriers.

The Effect of Rheumatoid Arthritis on Middle and Inner Ear Functions.

To investigate the middle and inner ear functions, and efferent auditory systems in patients with rheumatoid arthritis (RA). Thirty-five RA patients and 40 control subjects participated in the study. Pure-tone audiometry, high-frequency audiometry, multifrequency tympanometry, transient evoked otoacoustic emissions, and contralateral suppression tests were administered to all participants. Pure-tone hearing thresholds of RA patients were significantly higher at all frequencies except for 2000 Hz, 14,000 Hz, and 16,000 Hz in the right ear and 16,000 Hz in the left ear (p<0.05). Resonance frequency values of RA patients were statistically significantly lower than those of the control group (p<0.001). Emission amplitudes obtained with contralateral acoustic stimulation were significantly lower at 1400 Hz frequency in both groups than without contralateral acoustic stimulation (p<0.05). While contralateral suppression was observed at all frequencies in the control group, no suppression occurred at 2800 Hz and 4000 Hz in RA patients. The results obtained in this study demonstrated the presence of hearing dysfunction in patients with RA. When a patient is diagnosed with RA, an audiological evaluation should be made, and the patient should be informed about the possibility of audiological involvement.

Rough bed hydrodynamics under wave-current interactional flow field: double averaging approach

ABSTRACT The effect of surface wave on the double-averaged (DA) current-only flow properties is examined over two different types of rough beds comprising hemispherical elements with regular and staggered arrangements. For each case, the relative roughness spacing of p/r = 4 is maintained, where p = pitch distance and r = hemisphere height. The changes in turbulence characteristics against the water depth are analysed for superimposed surface waves propagating along the current with 1 and 2 Hz frequencies. The effect of regular and staggered roughness arrangements on different turbulence characteristics is explored. Results demonstrate that the DA stream-wise velocity decreases and increases at the outer region and within the roughness region, respectively, due to wave propagation. Owing to the surface wave, the DA intensity and form-induced intensity increase above the roughness layer and decrease within the bottom boundary layer. The values of form-induced intensities are smaller (greater) than the DA turbulence intensities at each considered flow depth within the roughness (outer) layer. For the entire flow depth, the values of DA Reynolds stress decrease due to wave interactions along a steady current. Again, the turbulence is observed to be close to isotropic for the current-only flow at the outer zone, while the flow transits to an anisotropic state with wave imposition on current. Further, the computed values of the turbulence indicator and dispersion functions are used for the identification of the level of turbulence and dispersion at each flow depth for the locations upstream and downstream of a hemisphere in the hemispherical bed.

Low-frequency noise attenuation through hybrid perforated hemispherical shells and membrane-type acoustic metamaterial

We propose a novel hybrid acoustic metamaterial that can achieve broadband sound insulation below 452 Hz and almost perfect sound absorption at 864 Hz. The metastructure was fabricated using additive manufacturing. Finite element method simulations were used to study the acoustic properties of the fabricated metamaterials. An equivalent electrical circuit was built using an electro-acoustic analogy to evaluate the sound absorption coefficient. The unique design of this meta-structure possesses two resonant frequencies. Low-frequency sound insulation is found due to the effective negative density at frequencies lower than the cutoff frequency of the elastic membrane. In contrast, a negative effective bulk modulus is the reason behind the broadband sound absorption. The theoretical and simulation results were validated through experiments. Experiments carried out showed an overall average sound transmission loss of 26.31 dB between 50 and 1600 Hz and 24.72 dB in the low-frequency zone (&amp;lt;400 Hz). Furthermore, over 69% of the sound intensity is absorbed in the 500–1000 Hz frequency range. The designed meta-structure exhibits broadband effective negative density below 452 Hz and effective negative bulk modulus from 864 to 1220 Hz. The design specifies a sample thickness of 3.8 cm, corresponding to a subwavelength thickness of approximately λ/10. As a result, the developed meta-structure can potentially be employed for sound insulation and absorption at low frequencies in the aerospace and automotive industries and in-room acoustic applications.

Detecting Geothermal Phenomena at Sikidang Crater Area Based on Ground Penetrating Radar Method

Abstract Sikidang Crater (2000 masl) is one of the leading tourist sites in the Dieng area. However, not many people realise that Sikidang Crater is located in the Dieng Volcanic Complex where most of the volcanoes belong to the type A category. Sikidang Crater is one of the main geothermal manifestations occupied by mud pools, solfatara, fumaroles, and altered rocks. These manifestations have potential geological hazards such as toxic gases, base surge, and mud explosions. On the other hand, it has a high level of human activity that increases the risk of disasters. As an effort to prevent and mitigate disasters, it is necessary to know the subsurface geological conditions of the crater. This study aims to determine the subsurface geological conditions of the crater. This research uses the methods of detailed geological mapping, manifestation observation, and Ground Penetrating Radar (GPR) geophysical survey. The tool used is Akula Geoscanner 9000C running at 64.75 Hz frequency, capable of penetrating 50 meters deep, and has a vertical resolution of &gt; 0.85 meters. Sikidang Crater is located at the southeastern foot of Mount Pangonan and it forms semicircular like a horseshoe. According to Van Zuidam, the crater is classified as a solfatara and fumarole field geomorphology unit. The lithology of this area is dominated by the eruption products of Mount Pangonan. In general, radargrams resulting from GPR surveys show various amplitudes of reflected electromagnetic waves. This is consistent when correlated with the variation in rock density of the research area. At some points, there is a decrease in electromagnetic wave velocity due to contrast in dielectric constant values, which is interpreted as geothermal fluid flow towards the surface. The potential level of geothermal fluid flow is classified into high, medium, low, and none based on the density of potential points seen in the radargram.

Impact of Cyclic Strain on Elastin Synthesis in a 3D Human Myometrial Culture Model.

The synthesis and assembly of mature, organized elastic fibers remains a limitation to the clinical use of many engineered tissue replacements. There is a critical need for a more in-depth understanding of elastogenesis regulation for the advancement of methods to induce and guide production of elastic matrix structures in engineered tissues that meet the structural and functional requirements of native tissue. The dramatic increase in elastic fibers through normal pregnancy has led us to explore the potential role of mechanical stretch in combination with pregnancy levels of the steroid hormones 17β-estradiol and progesterone on elastic fiber production by human uterine myometrial smooth muscle cells in a three-dimensional (3D) culture model. Opposed to a single strain regimen, we sought to better understand how the amplitude and frequency parameters of cyclic strain influence elastic fiber production in these myometrial tissue constructs (MTC). Mechanical stretch was applied to MTC at a range of strain amplitudes (5%, 10%, and 15% at 0.5 Hz frequency) and frequencies (0.1 Hz, 0.5 Hz, 1 Hz, and constant 0 Hz at 10% amplitude), with and without pregnancy-level hormones, for 6 days. MTC were assessed for cell proliferation, matrix elastin protein content, and expression of the main elastic fiber genes, tropoelastin (ELN) and fibrillin-1 (FBN1). Significant increases in elastin protein and ELN and FBN1 mRNA were produced from samples subjected to a 0.5 Hz, 10% strain regimen, as well as samples stretched at higher amplitude (15%, 0.5 Hz) and higher frequency (1 Hz, 10%); however, no significant effects because of third-trimester mimetic hormone treatment were determined. These results establish that a minimum level of strain is required to stimulate the synthesis of elastic fiber components in our culture model and show this response can be similarly enhanced by increasing either the amplitude or frequency parameter of applied strain. Further, our results demonstrate strain alone is sufficient to stimulate elastic fiber production and suggest hormones may not be a significant factor in regulating elastin synthesis. This 3D culture model will provide a useful tool to further investigate mechanisms underlying pregnancy-induced de novo elastic fiber synthesis and assembly by uterine smooth muscle cells.

Simulation Study on Mechanical Vibration Characteristics of GIS Bus Misalignment

Abstract Gas-insulated switchgear (GIS) has been widely used in power grid systems. The busbar is a crucial element within a GIS system, responsible for facilitating electrical connections between different GIS components. Any defects during manufacturing or improper installation procedures can lead to busbar misalignment, resulting in abnormal vibrations within the GIS. This study focuses on analyzing the mechanical vibration characteristics of GIS bus misalignment using a simulation model based on the finite element method for a 252kV GIS. When the busbar is misaligned and the conductor experiences an unbalanced force, it undergoes Coulomb forces at double the voltage frequency. Under normal or misaligned conditions, the conductor exhibits forced vibrations at the power frequency of 100Hz. However, when misalignment occurs, there is a coupling effect between electrodynamics and vibrations, causing the shell to display integer frequency vibration components.

Optimal design of low frequency rubber vibration isolator

Abstract Low frequency vibration isolator is a common structure vibration control device, widely used in transportation and machinery manufacturing fields. This paper is centered around enhancing the optimization design of rubber isolators operating at low frequencies. In the initial section, the paper introduces the structural framework and operational principles of rubber isolators. Furthermore, it delves into the analysis of their significance and potential applications within engineering contexts. Subsequently, the paper narrows its focus to address the design challenges concerning rubber isolators with low-frequency capabilities. A comprehensive three-dimensional model is constructed using ANSYS software, employing finite element analysis techniques. The paper employs a multi-objective optimization algorithm through a direct optimization module to facilitate the optimization design process. Within this study, the NSGA-II algorithm was employed to drive the optimization of design, with the objective of achieving a 10 Hz frequency for a series of dual rubber isolators, while simultaneously constraining static deformation to within 8 mm. The outcomes of this investigation emphasize the successful attainment of optimization goals by meticulously fine-tuning the structural dimensions of the isolators.