Objectives: to study the markers of endothelial dysfunction (ED): metabolites of Nitric Oxide (NO), endothelin1 (E1), homocysteine (HC), Von Willebrand factor (vWF), tissue plasminogen activator (tPA) in plasma of tobacco smokers and nonsmokers with essential hypertension (EH). Material and Methods: 124 hypertensive were recruited: 45 men and 79 women, with the mean age of 51,4±6,5 yrs and average duration of EH for 8,5±7,6 yrs. The control group included 25 healthy subjects (10 men and 15 women) with mean age of 48,2±7,8 yrs. Plasma NOx levels were determined by spectrometry, E1, HC, Wf, tPA levels by enzyme immunoassay. The results of study were analyzed by using Statistica 10.0. Results: to determine the effects of smoking on level of ED markers, we divided our subjects to subgroups: hypertensive smoker (35%), hypertensive nonsmokers (65%), smokers without hypertension (38%) and healthy nonsmokers (62%). In hypertensive smokers, in comparison to nonsmokers, we found a significant induction in NOx level (48,2±18,8 umol/l and 40,3±21,2 umol/l respectively, p<0,05), E1 (1,2±0,16 and 0,6±0,2 fmol/l respectively, p<0,05), HC (25,7±6,04 and 16,2±6,5 umol/l, respectively, p<0,05), vWF (1,3±0,8 and 1,1±0,6 mg/dl, respectively, p<0,05) and tPA (13,05±6,2 and 8,5±6,2 umol/l respectively, p<0,05). The NOx level correlated with smoking (r=0,46, p<0,05), HC level with smoking (r=0,4, p<0,05), and vWF level with the risk level of SCORE (r=0,43, p<0,05). In a controled group, the smokers, turned out to have significantly higher level of HC (20,7±5,3 and 17,2±4,7 umol.l, respectively, p<0,05), vWF (1,3±0,8 and 0,8±0,6 mg/dl, respectively, p<0,05) and tPA (11,1±6,5 and 6,6±5,2 umol/l, respectively, p<0,05). The changes in NOx and E-1 level were not significant. Conclusion: we observed an increase in level of ED markers in smokers with EH and of the control group. That confirms an important influence of smoking not only on development, but also progression of ED.