Hypericum Sampsonii Research Articles

Hyperisampsins N and O, two new benzoylated phloroglucinol derivatives from Hypericum sampsonii

Twenty-one benzoylated phloroglucinol derivatives bearing homoadamantyl frameworks were isolated from the aerial parts of Hypericum sampsonii, including two new ones named hyperisampsins N and O (1 and 2). The structures of 1 and 2 were elucidated by extensive NMR and mass spectrometric analyses. Their absolute configurations were further determined by using TDDFT ECD calculations. Compounds 2, 7, and 8 were evaluated for their cytotoxic activities against five human cancer cell lines, of which, 2 and 8 exhibited significant cytotoxic activities toward HL-60 cell and moderate activities against others cell lines.

Chiral separation and absolute configurations of two pairs of racemic polyprenylated benzophenones from Hypericum sampsonii

(±) Sampsonins A–B (1–2), two pairs of racemic polyprenylated benzophenones, were isolated from the aerial parts of Hypericum sampsonii and successfully separated by chiral HPLC column. Their structures were elucidated by spectroscopic analyses, X-ray diffraction analysis, and quantum chemical calculation of ECD method. Besides, the plausible biogenetic pathways of 1–2 were proposed, and all of them were evaluated for RXRα transcriptional-inhibitory activities and cytotoxicity against HeLa cells.

ChemInform Abstract: Hyperhexanone A, a Crucial Intermediate from Bicyclo[3.3.1]‐ to Cyclohexanone Monocyclic‐Polycyclic Polyprenylated Acylphloroglucinols.

AbstractTwo novel polycyclic polyprenylated acylphloroglucinol (PPAP) derivatives Hyperhexanone A (I) and B (II) are isolated from Hypericum sampsonii.

Hyperhexanone A, a crucial intermediate from bicyclo[3.3.1]- to cyclohexanone monocyclic-polycyclic polyprenylated acylphloroglucinols

Hyperhexanones A (1) and B (2), two novel polycyclic polyprenylated acylphloroglucinol (PPAPs) derivatives, were isolated from Hypericum sampsonii. Compound 1 possesses a novel 1,2-seco-bicyclo[3.3.1]-PPAP skeleton, which represents a crucial intermediate from bicyclo[3.3.1]-PPAPs to the peculiar cyclohexanone monocyclic-PPAPs (CM-PPAPs). A plausible biogenetic pathway was proposed to discuss the origins of the CM-PPAPs.

Polycyclic polyprenylated acylphloroglucinols: natural phosphodiesterase-4 inhibitors from Hypericum sampsonii

Seven novel PPAPs and 23 known analogs were isolated from Hypericum sampsonii, some of which were identified as potent PDE4 inhibitors.

Hypersampsones S–W, new polycyclic polyprenylated acylphloroglucinols from Hypericum sampsonii

Five new polycyclic polyprenylated acylphloroglucinols (1–5) with a bicyclo[3.3.1]nonane skeleton, along with five known analogues (6–10), were isolated from the aerial parts of Hypericum sampsonii.

Sampbenzophenones A–G, prenylated benzoylphloroglucinol derivatives from Hypericum sampsonii

Seven prenylated benzoylphloroglucinol derivatives, named sampbenzophenones A–G (1–7), together with two known analogues (8 and 9), were isolated from the aerial parts of Hypericum sampsonii.

Hyperisampsins H-M, Cytotoxic Polycyclic Polyprenylated Acylphloroglucinols from Hypericum sampsonii.

Six new polycyclic polyprenylated acylphloroglucinols (PPAPs), named hyperisampsins H–M (1–6), were isolated from the aerial parts of Hypericum sampsonii, together with five known analogs (7–11). The structures of 1–6 were established by extensive spectroscopic analyses, including HRESIMS and NMR. In addition, the absolute configurations of these new compounds were determined by electronic circular dichroism (ECD) calculations. Compounds 1 and 2 represent the first examples of PPAPs possessing a unique γ-lactone ring at C-23, while 3–6 differed from normal PPAPs with an unprecedented 1,2-dioxane ring. Compounds 1–7 were evaluated for their cytotoxic activities against a panel of human cancer cell lines in vitro, of which 3, 4, and 6 exhibited significant cytotoxic activities with IC50 values ranging from 0.56 to 3.00 μM. Moreover, compound 3 induces leukemia cell apoptotic death, evidenced by activation of caspase-3, degradation of PARP, up-regulation of Bax, and down-regulation of Bcl-2 and Bcl-xl.

ChemInform Abstract: Norsampsones A—D, Four New Decarbonyl Polycyclic Polyprenylated Acylphloroglucinols from Hypericum sampsonii.

Norsampsones A–D (1–4), four new decarbonyl polycyclic polyprenylated acylphloroglucinols, together with a new biogenetically related compound hypersampsone M (5), were isolated from the aerial parts of Hypericum sampsonii. Norsampsones A–D featured an unprecedented carbon skeleton with the loss of C-2 carbonyl in the phloroglucinol ring. All structures were determined by extensive NMR spectroscopic methods, ECD calculation, and single-crystal X-ray diffraction.

Dioxasampsones A and B, Two Polycyclic Polyprenylated Acylphloroglucinols with Unusual Epoxy-Ring-Fused Skeleton from Hypericum sampsonii

Dioxasampsones A and B (1 and 2), two new polycyclic polyprenylated acylphloroglucinols with an unusual epoxy-ring-fused skeleton by new ways of cyclization, along with a new nor-PPAPs hypersampson R (3) with the loss of C-31-33 in isopentenyl, were isolated from the aerial parts of Hypericum sampsonii. 1 possessed an unexpected hexacyclic skeleton with a rare 2,7-dioxabicyclo[2.2.1]heptane moiety, and 2 featured a unique tetrahydrofuro[3,4-b]furan-fused tricycle[4.3.1.1(5,7)]undecane skeleton. The gross structures of the new compounds were determined by extensive NMR spectroscopic methods. Their absolute configurations were deduced by single-crystal X-ray diffraction and ECD calculations.

Bioactive acylphloroglucinols with adamantyl skeleton from Hypericum sampsonii.

Hyperisampsins A-D (1-4), with tetracyclo[6.3.1.1(3,10).0(3,7)]tridecane skeletons and seven biogenetically related congeners (5-11), were isolated from Hypericum sampsonii. Their structures were elucidated by comprehensive spectroscopic techniques. The absolute configuration of 1 was established by ECD calculations, and those of 5 and 9 were confirmed by single X-ray crystallographic analyses. Hyperisampsins A and D showed potent anti-HIV activities with EC50 of 2.97 and 0.97 μM and selectivity index of 4.80 and 7.70, respectively.

Novel polyprenylated phloroglucinols from Hypericum sampsonii.

Hypericum sampsonii Hance (Clusiaceae) is a folk medicine used in Taiwan to treat blood stasis, relieve swelling, and as an anti-hepatitis drug. Two new polyprenylated phloroglucinol derivatives, hypersampsone R (1) and hypersampsone S (2), and a known prenylated benzophenone, hyperibone K (3) were isolated from the aerial parts of H. sampsonii. Their structures were determined by extensive 1D and 2D NMR, and MS spectral analyses.

Novel polycyclic polyprenylated acylphloroglucinols from Hypericum sampsonii

Four new polycyclic polyprenylated acylphloroglucinols with a tricyclo[4.3.1.15,7]undecane skeleton, hypersampsones N–Q (1–4), together with four know compounds (5–8), were isolated from the aerial parts of Hypericum sampsonii. Compound 1 bearing a rare 20, 25-epoxy group with the losing of C-21–23 in isopentenyl. An unusual 21, 24-epoxy group was found in the structure of 2. All structures were elucidated by extensive NMR spectroscopic methods. The absolute configurations of new compounds were determined by ECD calculation.

Novel polycyclic polyprenylated acylphloroglucinols from Hypericum sampsonii

Series of polycyclic polyprenylated acylphloroglucinols (PPAPs) have been isolated from the plants of Clusiaceae family, of which possess various biological activity.Hypericum sampsonii belongs to the Hypericum genus of Guttiferae family with abundant complex caged PPAPs, which exhibits anticancer activity, and has been used as a promising anticancer herb in Taiwan. In our efforts for more novel PPAPs, four new decarbonyl PPAPs norsampsones A-D(1 – 4), together with hypersampsone M (5), were isolated from the 60% EtOH extract of the aerial parts of H. sampsonii. Norsampsones A-D featured an unprecedented carbon skeleton with the loss of a carbonyl in phloroglucinol ring. All structures were determined by extensive NMR spectroscopic methods, ECD calculation, and single-crystal X-ray diffraction.Generally, most of the discovered PPAPs isolated from H. sampsonii form a unique family of structurally related caged metabolites, which were probably biosynthesized from a common precursor i. The plausible biosynthetic pathway to 1 – 5 was proposed. Norsampsones A-D (1 – 4) could be considered as the PPAPs with the loss of C-2 carbonyl in phloroglucinol ring. The plausible biogenetic pathway of 1 – 4 was also proposed to be generated from i through the Retro-Claisen and decarboxylation reaction. Hypersampsone M (5) was probably biosynthesized from the same precursor i by epoxidation, followed by intramolecular cyclization, oxidation, dehydration and reduction reaction.In addition, compounds 1, 3 and 4 were investigated for their effects on RXRα transcriptional-inhibitory activities using a reporter gene assay. Besides, their cytotoxicity against Hela cell were also evaluated. Compound 3 showed mild RXRα transcrip-tional-inhibitory activities as well as apopototic effects against Hela cell in a dose dependent manner.

Norsampsones A-D, four new decarbonyl polycyclic polyprenylated acylphloroglucinols from Hypericum sampsonii.

Norsampsones A-D (1-4), four new decarbonyl polycyclic polyprenylated acylphloroglucinols, together with a new biogenetically related compound hypersampsone M (5), were isolated from the aerial parts of Hypericum sampsonii. Norsampsones A-D featured an unprecedented carbon skeleton with the loss of C-2 carbonyl in the phloroglucinol ring. All structures were determined by extensive NMR spectroscopic methods, ECD calculation, and single-crystal X-ray diffraction.

Differential Expression of Benzophenone Synthase and Chalcone Synthase in Hypericum Sampsonii

cDNAs encoding Hypericum sampsonii benzophenone synthase (HsBPS) and chalcone synthase (HsCHS) were isolated and functionally characterized. Differential expressions of HsBPS and HsCHS were monitored using quantitative polymerase chain reaction (PCR). In the vegetative stage, HsBPS was highly expressed in the roots; its transcript level was approx. 100 times higher than that of HsCHS. Relatively high transcript amounts of HsBPS were also detected in older leaves, whereas the youngest leaves contained higher transcript amounts of HsCHS. In the reproductive stage, maximum HsCHS expression was detected in flowers, the transcript level being approx. 5 times higher than that of HsBPS. The inversed situation with a 10-fold difference in the expression levels was observed with fruits. High transcript amounts for both proteins were found in roots.

Prenylated phloroglucinol derivatives from Hypericum sampsonii

Six new acylphloroglucinol derivatives, sampsonols A-F (1–6), were isolated from the petroleum ether extract of the aerial parts of Hypericum sampsonii. The structures and relative configurations of sampsonols A-F were elucidated by extensive spectroscopic analyses. All these compounds were tested for their in vitro cytotoxic and anti-inflammatory activities. Sampsonols A and B (1 and 2) showed significant cytotoxicity against four human tumor cell lines with IC50 values in the range of 13–28μM, whereas sampsonols C and F (3 and 6) showed potent inhibitory activities against LPS-induced NO production in RAW 264.7 macrophages with IC50 values of 27.3 and 29.3μM, respectively.

Four geranyl-bearing polyisoprenylated benzoylphloroglucinol derivatives from Hypericum sampsonii

Four new geranyl-bearing polyisoprenylated benzoylphloroglucinol derivatives, hypersampsone I (1), hypersampsone J (2), and hypersampsone K (3), together with hypersampsone L (4) which possessed a novel skeleton, were isolated from the fruit of Hypericum sampsonii. Their structures were determined on the basis of spectroscopic data, mainly 1D- and 2D-NMR spectroscopy and mass spectrometry. The structures of 1 and 2 were confirmed by X-ray crystallographic analysis.

Two Unusual Phenolic Substances and One New Xanthone from Hypericum sampsonii

AbstractSampsone A (1), a novel prenylated aromatic lactone, and sampsone B (2), an unusual dihydrodibenzodioxinone, together with sampsone C (3), a new xanthone, were isolated from the aerial parts of Hypericum sampsonii. Their structures were determined by spectroscopic methods which were mainly 1D‐ and 2D‐NMR techniques, and the structure of sampsone B (2) was also confirmed by X‐ray crystallographic analysis. All of these compounds were evaluated for in vitro antibacterial activity against methicillin‐resistant Staphylococcus aureus (MRSA). Only sampsone A showed moderate antibacterial activities at a minimum inhibitory concentration (MIC) of 32 μg/ml.

Two New Xanthones from Hypericum sampsonii and biological activity of the isolated compounds

Phytochemical investigation of the CH(2) Cl(2) extract of the aerial part of Hypericum sampsonii yielded two new prenylated xanthones, hypericumxanthone A and B, together with three known xanthones. Their structures were elucidated by analysis of physical and spectral (UV, IR, mass and NMR) data and comparison of spectroscopic data with those reported previously. All these compounds were evaluated for in vitro antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Two new compounds were also tested for their cytotoxicity against human breast (MCF-7), hepatoma (HepG2), colon (HT-29) and lung (A549) tumour cell lines. Two new compounds showed moderate antibacterial activities at minimum inhibitory concentrations (MIC) of 16 and 32 µg/mL, respectively, whereas the positive standard antibacterial drug, vancomycin, showed an MIC of 8 µg/mL. The other compounds were inactive against MRSA. In addition, hypericumxanthone B showed weak inhibitory activities against four human tumour cell lines.