Hypericum Perforatum Extract Research Articles

Protective Effect of Hypericum perforatum Extract on Gentamicin Induced Nephrotoxicity in Mice

Protective Effect of Hypericum perforatum Extract on Gentamicin Induced Nephrotoxicity in Mice

Urtica dioica leaves modulates hippocampal smoothened-glioma associated oncogene-1 pathway and cognitive dysfunction in chronically stressed mice.

The present study was aimed to evaluate the effect of Urtica dioica (UD) extract against chronic unpredictable stress (CUS)-induced associative memory dysfunction and attempted to explore the possible mechanism. Male Swiss albino mice (25-30g) were divided into six groups, viz. group-I received 0.3% carboxymethyl cellulose and served as control (CTRL), group II was exposed to CUS (21days) and received vehicle (CUS), group III was subjected to CUS and received Hypericum perforatum extract (350mg/kg, p.o.) (CUS+HYP), group IV received Hypericum perforatum extract (350mg/kg, p.o.) (CTRL+HYP); group V was subjected to CUS and received UD extract (50mg/kg, p.o.) (CUS+UD), group VI received UD extract (50mg/kg, p.o.) (CTRL+UD). CUS significantly induced body weight loss (p<0.05) and associative memory impairment in step down task (p<0.05) as compared to control mice. CUS significantly downregulated Smo (p<0.05), Gli1 (p<0.01), cyclin D1 (p<0.05), BDNF (p<0.01), TrKB (p<0.01) and MAPK1 (p<0.01) mRNA expression in hippocampus as compared to control mice. CUS significantly increased the levels of TBARS (p<0.01) and nitric oxide (p<0.001), and decreased catalase (p<0.001) and total thiol (p<0.01) in plasma resulting in oxidative stress and inflammation. Chronic UD administration significantly reverted CUS mediated body weight loss (p<0.05) and cognitive impairment (p<0.05). UD administration significantly decreased the levels of TBARS (p<0.01) and nitric oxide (p<0.05), and increased the levels of catalase (p<0.01) and total thiol (p<0.05) in plasma. Chronic UD administration significantly upregulated hippocampal Smo (p<0.05), Gli1 (p<0.001), cyclin D1 (p<0.05), BDNF (p<0.05), TrKB (p<0.05) and MAPK1 (p<0.05) in stressed mice. Further, UD extract did not reverse cyclopamine induced downregulation of Gli1 and Ptch1 mRNA in hippocampal slices. UD modulated Smo-Gli1 pathway in the hippocampus as well as exerted anti-inflammatory and antioxidant effects. UD extract might prove to be effective for stress mediated neurological disorders.

Effects of Hypericum perforatum extract on the endocrine immunenetwork factors in the immunosuppressed Wistar rat

The study aimed to investigate the effects of Hypericum perforatum extract on the endocrine immune network factors in the immunosuppressed rat. Wistar rats were used to establish an immunosuppression model. Immunosuppressed rats received Hypericum perforatum extract suspension for 7 days at doses of 600 mg/kg (containing hyperforin at 7.2mg/kg).Endocrine immune network factors including IL-1, DA, NE, and 5-HT were determined on day 8.The results indicated that Hypericum perforatum extract affects the endocrine immune network through decreasing endogenous IL-1,dopamine (DA), norepinephrine (NE), and 5- hydroxytryptamine (5-HT) levels. Hyperforin reverses immune suppression through enhancing DA levels to inhibit B cells while promoting T cell and macrophage functions to recover immune function.

Evaluation of poly ε-caprolactone electrospun nanofibers loaded with Hypericum perforatum extract as a wound dressing

In this study, for the first time, a biocompatible and non-toxic herbal wound dressing was made by encapsulation of Hypericum perforatum alcoholic extract at different concentrations (10, 30 and 50 % v/v) into poly e-caprolactone electrospun nanofibers to achieve an effective wound dressing. The electrospinning processing parameters such as needle tip to collector distance, applied voltage and flow rate of feed solution were changed until accumulated nano-scale fibers without bead structures were obtained. Then, the morphology of the nanofibrous structures was investigated by scanning electron microscopy. After synthesis of the nanofibers in optimal conditions, the antibacterial activity of the optimized bandages was investigated by the disc diffusion method against strains of Staphylococcus aureus and Escherichia coli. The release content of the herbal drug was tested by the total immersion method in phosphate buffer saline and displayed a constant drug liberation with time. Water vapor transmission rate for the wound dressing was evaluated by pseudo-extra cellular fluid for optimal samples. The crystallinity and thermal behavior of the mats with and without H. perforatum alcoholic extract were studied by X-ray diffraction and differential scanning calorimetry. The results of antibacterial activity, cell culture and In vitro methyl thiazolyl tetrazolium assays demonstrated these unique structures as being very useful as burn and ulcer dressings.

Cytoprotective and anti-inflammatory effects of STW 3-VI (Hypericum perforatum extract) in vitro

Inflammation and glutamate toxicity play an integral role in a variety of disorders as depression. Additionally, a large body of evidence indicates that adaptation to chronic stress involves response from both, the neuroendocrine and immune systems. Depression also has been linked to an inflammatory response since pro-inflammatory cytokines like interleukin 6 (IL-6) and tumor necrosis factor (TNFα) are increased in depressed patients. In this context, the combined antioxidant and anti-inflammatory properties of Hypericum perforatum (St. John's wort) extract is suggested to contribute to the antidepressant effects by normalization of an overactive hypothalamic-pituitary-adrenal axis. Thus, the aim of our in vitro investigations is to determine the effects of STW3-VI on protection of differentiated mouse hippocampal HT22 cells from the cytotoxic effects of glutamate or NMDA and the possible anti-inflammatory properties on LPS-activated macrophages (MΦ).

Antioxidant activity and rosmarinic acid content of ultrasound-assisted ethanolic extracts of medicinal plants

The interest in finding natural antioxidants for use in food, drugs or cosmetic products has increased concerning the replacement of synthetic antioxidants due to their possible undesirable toxic effects on humans long-term exposed.Ethanolic extracts of Hypericum perforatum (Hypericaceae), Lavandula angustifolia (Lamiaceae), Malva sylvestris (Malvaceae), Melissa officinalis (Lamiaceae), Salvia officinalis (Lamiaceae), Rosmarinus officinalis (Lamiaceae) were obtained under sonication with a yield of 5.9%, 3.8%, 5.8%, 3.8%, 4.2% and 2.3% (w/w), respectively and their antioxidant activity assessed by the TLC-DPPH and DPPH assays. With exception of L. angustifolia and M. sylvestris extracts all the others showed antioxidant activity greater than 70% (at 0.1mg/mL).The HPLC analysis shows that all tested extracts contain rosmarinic acid and suggest that H. perforatum extract have the highest concentration (2.76mM) and M. sylvestris extract the lowest one (0.47mM).In this study, we describe a simple laboratory procedure using an ultrasound-assisted ethanolic extraction from six medicinal plants. This potential didactic approach showed to be a rapid and effective process on preserving the antioxidant activity and rosmarinic acid content. The HPLC system was used to evaluate the ability to assess rosmarinic acid content.

Ethanol Extracts of Achillea millefolium and Hypericum perforatum Low Anti-Toxoplasma Activity

Objectives:This study was performed to determine the lethal and the inhibitory effects of ethanol extracts of Achillea millefolium (A. millefolium) and Hypericum perforatum (H. perforatum) on Toxoplasma gondii (T. gondii) RH strain tachyzoites in vitro.Methods:The tachyzoites were treated with concentrations of 10, 50, and 100 mg/mL of A. millefolium and H. perforatum extracts within 10, 30, and 45 minutes in the wells. The mortality rates of tachyzoites treated with extracts were determined by using alkaline methylene blue staining. Also, the tachyzoites in cell cultures were treated with concentrations of 50, 100, and 200 mg/mL of these extracts. The cell viability, inhibition concentration (IC50), and selectivity were determined from MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays.Results:In the cell-free in vitro study, all of tachyzoites were killed at concentrations of 100 mg/mL of both extracts while at concentration 10 mg/mL, the mortality was 4.53% − 5.31%. In the cell culture study, the values of the effective concentration (EC50) were 215 and 153 μg/mL and the selectivities were 0.73 and 0.69 for the A. millefolium and the H. perforatum extracts, respectively.Conclusion:We conclude that neither extracts has any significant effect on the tachyzoites of T. gondii in cell cultures.

Hyperforin production in Hypericum perforatum root cultures

Extracts of the medicinal plant Hypericum perforatum are used to treat depression and skin irritation. A major API is hyperforin, characterized by sensitivity to light, oxygen and temperature. Total synthesis of hyperforin is challenging and its content in field-grown plants is variable. We have established in vitro cultures of auxin-induced roots, which are capable of producing hyperforin, as indicated by HPLC-DAD and ESI–MS analyses. The extraction yield and the productivity upon use of petroleum ether after solvent screening were ∼5mg/g DW and ∼50mg/L culture after six weeks of cultivation. The root cultures also contained secohyperforin and lupulones, which were not yet detected in intact plants. In contrast, they lacked another class of typical H. perforatum constituents, hypericins, as indicated by the analysis of methanolic extracts. Hyperforins and lupulones were stabilized and enriched as dicyclohexylammonium salts. Upon up-scaling of biomass production and downstream processing, H. perforatum root cultures may provide an alternative platform for the preparation of medicinal extracts and the isolation of APIs.

PROTECTIVE EFFECTS OF HYDROALCOHOLIC EXTRACT OF HYPERICUM PERFORATUM AGAINST BLEOMYCIN-INDUCED PULMONARY FIBROSIS IN RATS

BACKGROUND AND OBJECTIVE: Pulmonary fibrosis is a chronic interstitial lung disease caused by parenchymal lung damage due to inflammatory factors and fibrosis. Hypericum perforatum contains various flavonoids with antioxidant properties. This study aimed to evaluate protective effects of hydroalcoholic extract of Hypericum against bleomycin-induced pulmonary fibrosis in rats. METHODS: This empirical study was conducted on 30 Wistar rats weighing 150-180 grams. Animals were randomly divided into five groups of six. Group one received a single dose of normal saline intraperitoneally, and group two was administered with bleomycin (7.5 units per kg) intratracheally. Other groups received daily doses of Hypericum extract (50, 100 and 200 mg/kg) via intraperitoneal injection one week before and two weeks after bleomycin administration. After 21 days, animals were sacrificed, and blood and lungs were collected for histopathological examinations, and measurement of plasma malondialdehyde (MDA) and lung hydroxyproline (HP). FINDINGS: In this study, lung index, HP and plasma MDA in normal saline group were respectively 7.09±0.32 mg per kilogram of body weight, 1.80±0.23 mg per gram of lung tissue, and 1.32±0.27 micromoles per liter of plasma. In rats administered with bleomycin, these values were 9.75±0.90, 5.43±0.7 and 3.04±0.42, respectively. Treatment with Hypericum extract, especially at dosage of 200 mg/kg, resulted in a significant reduction in the aforementioned parameters compared to bleomycin group (p<0.05). CONCLUSION: According to the results of this study, hydroalcoholic extract of Hypericum perforatum could exert protective effects against bleomycin-induced pulmonary fibrosis.

Effect of Hypericum perforatum Aqueous Extracts on Serum Lipids, Aminotransferases, and Lipid Peroxidation in Hyperlipidemic Rats.

Background:Patients with high levels of total cholesterol (TCH), low-density lipoprotein cholesterol (LDL-CH), and triglyceride (TG) are at increased risk of coronary heart disease. Studies have shown that flavonoids and antioxidant compounds have beneficial effects on hyperlipidemia.Objectives:The aim of the present study was to evaluate the effects of extract of Hypericum perforatum (EHP) on the serum lipid profile (TCH, TG, and LDL-CH), aminotransferase, alkaline phosphatase, and lipid peroxidation in hyperlipidemic rats.Materials and Methods:Thirty-two male rats weighting 200 ± 10 g were randomly divided into four experimental groups: 1) control, 2) control + EHP, 3) hyperlipidemia, and 4) hyperlipidemia + EHP. The rats in the hyperlipidemic groups were fed a high-fat diet for 60 days, and EHP (300 mg/kg) was injected intraperitoneally for 2 weeks in the rats in the second and fourth groups. At the end of the experimental period, blood samples from each group were analyzed.Results:There was a significant reduction in LDL-CH in the control + EHP group and the hyperlipidemia + EHP group (P < 0.05). TCH was significantly reduced in the control + EHP group (P < 0.05). There were no significant changes in the levels of TG and HDL-CH. Malondialdehyde, aspartate aminotransferase, and alanine aminotransferase were significantly reduced in the hyperlipidemia + EHP group (P < 0.05), with no significant change in alkaline phosphatase.Conclusions:EHP was able to both reduce LDL-CH and to significantly decrease markers of oxidative stress and lipid peroxidation induced by hyperlipidemia. Therefore, this herb, as a new pharmacological component, could be used to reduce certain blood lipids, lipid peroxidation, and aminotransferase markers.

Effects of Hypericum perforatum extract and its main bioactive compounds on the cytotoxicity and expression of CYP1A2 and CYP2D6 in hepatic cells.

Hypericum perforatum (H. perforatum) is one of the most used medicinal plants. However, it has been associated with relevant interactions with several drugs. This situation is probably mediated by cytochrome P450 enzymes (CYP450), namely the 1A2 (CYP1A2) and 2D6 (CYP2D6) isoforms This study aims to assess the cytotoxic and CYP1A2 and CYP2D6 inductive and/or inhibitory effects of a H. perforatum extract and its main bioactive components in hepatic cell lines. A MTT proliferation assay was performed in WRL-68, HepG2 and HepaRG cells after exposition to different concentrations of H. perforatum extract, hypericin and hyperforin for 24 and 72 h. Then, a real-time PCR analysis was accomplished after incubating the cells with these products evaluating the relative CYP1A2 and CYP2D6 expression. These products have relevant cytotoxicity at a 10 μM concentration and it was also demonstrated for the first time that H. perforatum can lead to a significant CYP1A2 and CYP2D6 induction in all cell lines. Moreover, hypericin seems to induce CYP1A2 in HepG2 cells and to inhibit its expression in HepaRG cells while hyperforin induced CYP1A2 in HepG2 and in WRL-68 cells. Additionally, hypericin and hyperforin induce CYP2D6 in HepG2 cells but inhibits its expression in HepaRG and in WRL-68 cells. This study not only evidenced that H. perforatum extract and two of its bioactive components can have toxic effects in hepatic cell lines but also emphasized the potential risk of the consumption of H. perforatum with CYP1A2- and CYP2D6-metabolized drugs.

Clinical study data on the safety of isopropanolic extract of Cimicifuga racemosa allow for its unlimited use according to ICH-E1

The duration of use of medicinal products intended for non-life-threatening diseases is directly linked to a positive risk/benefit assessment based on clinical data over distinct exposure periods according ICH-E1 [1]. The HMPC monograph on Cimicifuga racemosa (CR) addressing ethanolic and isopropanolic extract of CR [2] states “Cimicifuga should not be taken for more than 6 months without medical advice”. Based on the ICH-E1-criteria, sufficient clinical study data are available to fulfill the ICH-E1-requirements for a safe long-term treatment – i.e. longer than 6 months – at least for the isopropanolic extract (iCR). We provide a reflection according to ICH-E1 on the product safety data from clinical studies on iCR, stratified if alone or in combination with a distinct ethanolic extract of Hypericum perforatum (eHP).

Brain Uptake of Tetrahydrohyperforin and Potential Metabolites after Repeated Dosing in Mice.

Tetrahydrohyperforin (IDN-5706) is a semisynthetic derivative of hyperforin, one of the main active components of Hypericum perforatum extracts. It showed remarkable positive effects on memory and cognitive performances in wild-type mice and in a transgenic mouse model of Alzheimer's disease, but little was known about the concentrations it can reach in the brain. The investigations reported herein show that repeated treatment of mice with tetrahydrohyperforin (20 mg/kg intraperitoneally, twice daily for 4 days and once on the fifth day) results in measurable concentrations in the brain, up to 367 ng/g brain (∼700 nM) 6 h after the last dose; these concentrations have significant effects on synaptic function in hippocampal slices. The other main finding was the identification and semiquantitative analysis of tetrahydrohyperforin metabolites. In plasma, three hydroxylated/dehydrogenated metabolites were the largest (M1-3) and were also formed in vitro on incubation of tetrahydrohyperforin with mouse liver microsomes; the fourth metabolite in abundance was a hydroxylated/deisopropylated derivative (M13), which was not predicted in vitro. These metabolites were all detected in the brain, with peak areas from 10% (M1) to ∼1.5% (M2, M3, and M13) of the parent compound. In summary, repeated treatment of mice with tetrahydrohyperforin gave brain concentrations that might well underlie its central pharmacological effects. We also provide the first metabolic profile of this compound.

The effect of Hypericum perforatum L. (St. John's Wort) on prevention of myringosclerosis after myringotomy in a rat model

ObjectivesThe purpose of this study was to identify the possible effects of Hypericum Perforatum (HP) on the prevention of experimentally induced myringosclerosis (MS). MethodsTwenty eight Wistar Albino rats were used and they were divided into four groups. Tympanic membranes of all animals were perforated and then group I had no treatment as a control group, group II had treated with olive oil only, group III had treated with HP orally and group IV had treated with HP topically. ResultsGroups I and II showed extensive myringosclerosis in contrast to those of Groups III and IV which had significantly less changes (p<0.05). The inflammation and fibrosis in the lamina propria of the tympanic membranes of Groups I and II were found to be significantly more pronounced (p<0.05). The tympanic membranes were found to be significantly thinner in Groups III and IV when compared with Groups I and II (p<0.05). ConclusionsThe results of the present study suggested that oral or topical administration of HP extract after myringotomy suppressed the inflammation and fibroblastic activity in the lamina propria of the myringotomized TMs of the rats. Further clinical studies with larger population using HP and other antioxidants will be essential to provide further evidence for use of antioxidant therapy in patients with myringotomy and ventilation tube insertion for otitis media with effusion.

In vitro Bacteriostatic Effect of a Scaffold with a Mixture of Hypericum perforatum and Azadirachta indica Oil Extracts

In vitro Bacteriostatic Effect of a Scaffold with a Mixture of Hypericum perforatum and Azadirachta indica Oil Extracts

Immunomudulatory effects of hydroalcoholic extract of Hypericum perforatum.

Hypericum perforatum (St. John's Wort) has long been used in traditional medicine to treat a variety of internal and external ailments. The present study was done to evaluate the immumodulatory potentials of the hydroalcoholic extract of H. perforatum. Twenty male BALB/c-mice were randomly allocated in two equal groups and immunized with sheep red blood cells (SRBCs) and complete Freund's adjuvant. Mice in the treatment group orally received hydroalcoholic extract of H. perforatum (110 mg/Kg daily) from the beginning of the study which continued for 2 weeks. The data indicated a significant increase in the level of anti-SRBC antibody and simultaneously a significant decrease in the level of cellular immunity, an enhancement in foot pad thickness, in treatment group compared to control group. The level of the respiratory burst in phagocytic cells and the level of lymphocyte proliferation in splenocytes were significantly decreased in the treatment group compared to control group. Moreover, extract caused a significant reduction in the production of pro-inflammatory IL-17 as well as IFN-γ, parallel to increasing the level of IL-6. The hydroalcoholic extract of H. perforatum may be used as a natural source for treatment of immunopathologic conditions.

EFFECT OF POULTRY FEED SUPPLEMENTED WITH HYPERICUM PERFORATUM EXTRACT AND VIRGINIAMYCINE ON GROWTH PERFORMANCE, SOME IMMUNE RESPONSES AND INTESTINAL MICROBIAL POPULATION OF BROILERS

This study investigated the effect of adding Hypericum perforatum to drinkable water in comparison to virginiamycine on performance of broilers and thus on improvement and increase welfare and growth. A total of 400 , one day old broiler chicks with an average weight of 37±50 g were divided into 5 treatment groups and were further subdivided into 4 replicates. The treatments were divided as basal diet with no Hypericum perforatum extract kept as control, and for others 150 mg/l (S1), 200 mg/l (S2) and 250 mg/l (S3) of herbal extract in their Drinking water and virginiamycine (S4) were used respectively. The live body weight gains and feed consumption of birds were measured individually feed conversion efficiency were calculated weekly. At the end of the trial for investigating the effect of using Hypericum perforatum and virginiamycine on performance of broilers, 3 birds form each replicates were slaughtered and some blood samples were taken for determination HDL, LDL, Triglyceride levels and antibody titer against Newcastle vaccine. Data showed that use of S1, S2 and S3 and S4 increased FI significantly (P<0.05) in comparison to control. Also BW and Pre-slaughter weigh at the end of the experiment were also significantly higher (P<0.05) in experimental groups compared to the control. Liver percentage was significantly induced were broilers fed with experimental diets (p<0.05). There were significant differences (p<0.05) for heart percentage between treatments. Using of herbal plant extract could reduce abdominal fat percentage statistically (p<0.05).Percentage of gizzard was higher in the control group and it was at the lowest in virginiamycine group (p<0.05). triglyceride, cholesterol and LDL level decreased significantly in the treated groups while HDL level increased significantly in S1, S2 ,S3and S4. Antibody titers were significantly higher (p<0.05) when broilers were fed by S1, S2, S3and S4. It was determined that herbal and virginiamycine had significant effects on HI test (p<0.05) that compared to control group. Data showed that E. coli population decreased in S1, S2, S3 and S4 while Lactobacillus population increased significantly in S4, S3,S2 and S1 respectively (p<0.05).

Effects of Hypericum perforatum extract on IgG titer, leukocytes subset and spleen index in rats.

Hypericum perforatum L. is a medicinal plant containing many polyphenolic compounds, ‎including flavonoids and phenolic acids with antidepressant and anti-inflammatory properties. ‎This study was investigated the effects of Hypericum perforatum extract (HPE) on immunity, ‎body weight (BW), and spleen index (SI) in rats.‎ A total of 24 Wistar male rats were randomly received 4 different doses (6 rats each) of HPE ‎‎(0, 100, 200 and 400 mg/kg BW) intraperitoneally for 14 days using a completely ‎randomized design. On days 1 and 7, rats were received 0.5 ml SRBC (10%) injection. Blood ‎samples were collected on day 14 to evaluate IgG titer and leukocyte count. On days 1, 7 and ‎‎14, the BW and on day 14 spleen were weighted for SI. ‎ The IgG titer increased with higher doses of HPE. The HPE increased number of ‎lymphocytes at 200 mg but decreased at 400 mg, number of neutrophils decreased at 200 mg ‎but increased at 400 mg, and number of monocytes increased at 100 mg and 200 mg but ‎decreased at 400 mg (p<0.01). Increasing doses of HPE lowered BW (p<0.01). ‎The HPE increased SI at 100 mg and 200 mg but decreased at 400 mg (p>0.072). ‎ The results showed that HPE slightly improved IgG titer but significantly increased ‎the number of leukocytes and monocytes at 200 mg, and neutrophils at 400 mg. The HPE ‎decreased BW at 100 mg and 200 mg with no damage on spleen. ‎

Protection of human HepG2 cells against oxidative stress by a methanolic extract of Hypericum perforatum elicited with Agrobacterium tumefaciens

Protection of human HepG2 cells against oxidative stress by a methanolic extract of Hypericum perforatum elicited with Agrobacterium tumefaciens

The Healing Effect of Hypericum perforatum Extract on Acetic Acid-Induced Ulcerative Colitis in Rat

Background Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD). There are several chemical and herbal drug regimens for treatment of UC. Objectives The aims of this study were to investigate the effects of Hypericum perforatum on histopathological and tissue malondialdehyde (MDA) level of colonic tissue in rat with induced UC. Materials and Methods Two milliliters of 3% acetic acid was administered into the colon to induce UC. Seventy rats were divided into seven equal groups. Groups I and II received 1 mL of 600 and 300 mg/kg H. perforatum extract orally per day respectively; groups III and IV received 1 mL of 20% and 10% intra-colonic gel form of H. perforatum extract daily respectively; group V as positive control received 2 mL of intra-colonic asacol; group VI was a negative control receiving 0.5 mL/kg of normal saline after induction of UC; group VII received just intra-colonic gel base. All the animals were evaluated for histological changes and tissue MDA level seven days after the treatment. Results H. perforatum extract in the two forms of trans-rectal and oral administration on the seventh day after the therapy could result in a more healing effect on acetic acid-induced damaged colonic tissue with a reduction in the MDA activity. In trans-rectal administration, the 20% gel form had a better healing response than the 10% gel form and was prominently more effect on the seventh day of the therapy. In oral administration of strawberry extract, the 600 mg/kg dosage had a better healing response than the 300 mg/kg and was significantly more effective on the seventh day of therapy. Conclusions So H. perforatum may be considered as a treatment of choice for UC especially in gel form to broaden the current therapy options of the disease.