Mother-pup interactions during early life serve to program the circuitry orchestrating the diverse responses to stress in the offspring [1,2,3]. Long periods of daily separation from the mother (ie, 3–6 h) alter behavioural, neural, and endocrine responses to stress which persist throughout life, owing to altered development and regulation of neurones orchestrating the stress response [3,4]. These neurones include the paraventricular nucleus of the hypothalamus (PVH) and noradrenergic neurones of the locus coeruleus (LC) [3,5]. Because neurones affected by maternal separation also play an important role in ventilatory control, we tested the hypothesis that maternal separation disrupts development of the respiratory control system. This study 1)compared ventilatory responses to hypercapnia between control animals and rats subjected to maternal separation during the neonatal period, and 2)determined whether hypercapnic activation of LC and PVH neurones (as indicated by Fos mRNA expression) is affected by neonatal separation. Newborn rats were separated from their mother 3 h/day for 10 consecutive days (P3–P12). Pups were then reared in standard conditions until adulthood. Ventilatory activity was measured in awake rats using whole body plethysmography under baseline (normoxic normocapnia) and hypercapnic conditions (5% CO2 in air). At the end of the experiment, animals were sacrificed and brains were collected for subsequent quantification of Fos mRNA in the PVH and LC by in situ hybridization. Plethysmographic measurements show that the hypercapnic venti-latory response is 30% lower in separated rats (n = 6) than in controls (n = 13). This attenuation of the response is due exclusively to a reduced increase in tidal volume, as the frequency component of the response was not affected by the neonatal treatment. In separated rats, the blunted responsiveness to hypercapnia was associated with a lack of increase in Fos mRNA levels in the PVH. However, hypercapnic exposure nearly doubled Fos mRNA expression in control rats, thereby suggesting that PVH neurons play a role in the hypercapnic ventilatory response. Conversely, hypercap-nia decreased Fos mRNA in the LC equally in both groups. These data suggest that a neonatal stress, such as disruption of mother-infant interactions, which is not directly relevant to respiratory homeostasis may have a significant impact on development of the hypercapnic ventilatory response. These results may point to a new factor in the etiology of delays in developmental maturation in respiratory control and subsequent of respiratory disorders.