Control of ventilation in the hypercapnic skate Raja ocellata: II. Cerebrospinal fluid and intracellular pH in the brain and other tissues

Abstract

This study examined the possible role(s) of central-base stimuli in the increase in ventilation induced by hypercapnia in the skate, a response that is not due to an O 2 signal (Graham et al., Respir. Physiol., 1990, 80: 251–270). Skate were sampled for cerebrospinal fluid (CSF) acid-base status, intracellular pH of the brain ( 14C-DMO method), and pHi in other tissues throughout 24 h of exposure to Pi CO 2 = 7.5 Torr . CSF P CO 2 rapidly equilibrated with the elevated Pa CO 2 . Despite the much lower non-HCO 3 −1 buffer capacity in the CSF, CSF pH was not depressed to the same extent as blood pHa. CSF pH was also regulated more rapidly, returning to control levels by 8–10 h, whereas pHa remained significantly depressed at 24 h. Similarly, the pHis of the weakly buffered brain and heart ventricle were initially compensated more rapidly than those of more strongly buffered white muscle and red blood cells. However, brain pHi adjustment slowed markedly after 4 h and stabilized at only 70% compensation by 20–24 h, suggesting that brain intracellular acidosis may play a role in the long-term increase in ventilation. CSF and brain were the only compartments which did not exhibit an apparent compounding metabolic acidosis during the initial stages of hypercapnic exposure. While these results illustrate the primacy of central acid-base regulation, they do not support a role for CSF pH in the long-term elevation of ventilation in response to hypercapnia. Depressions in pHa and brain phi appear the two mosly likely candidates for proximate stimuli.