The use of activated carbon in adsorbing organic compounds inherent in human urine and subsequently desorbing them as nutrients for hydroponic solutions has been examined in this study. Human urine was collected for adsorption and desorption. The adsorbent was characterized in terms of porosity, surface area, SEM images, and FTIR spectrum. After being washed, activated carbon was set into columns. Urine was subsequently pumped in with 3 distinct urine concentrations of 100%, 50%, and 20% for adsorption during sixteen hours. Afterward, the activated carbon was removed from the columns and was settled at the ambient temperature in 3 days to decompose adsorbed organic compounds, producing constituents that facilitate absorption in plants. For desorption, the three kinds of dried activated carbon from adsorption was crammed into columns for a desorption process in sixteen hours. To evaluate adsorption and desorption, TOC, IC, NH 4 + , NO 3 − , and P 2 O 5 were the parameters recorded during experimenting and resulted in highest numbers of 1610 mg/l, 326.30 mg/l, 2.61 mg/l, 7.24 mg/l, and 256.87 mg/l, respectively. The study also obtained EC and pH of the columns with the highest numbers of 16630 μ S/cm and 9.48, respectively. Nutrients gained from desorption were tested for the cultivation of Brown mustard (Brassica juncea) in hydroponics. The nutrient resulting from the 50% urine generated the most productive parameters of growth compared to the rest. Through this study, it is clear that the application of adsorption–desorption in agricultural production is completely feasible in terms of practical implementation. • Hydroponic solution was produced from Human urine via adsorption–desorption. • Brassica juncea was grown well in hydroponic solution derived from 50% urine. • EC, pH, TOC, IC, NH 4 + , NO 3 − , and P 2 O 5 were used to evaluated hydroponic solution. • Mechanism of hydroponic solution production involved ion exchange between H + and OH − .