The abundance of atopic dermatitis among children is 5–20 % and among adults is 2–10 %. One of the advanced solutions is the development of drugs, based on phytosubstances. One of the well-known medical plants for skin treatment is Bidens tripartita (Asteraceae). it is known for its anti-inflammatory and regenerating properties. On the base of SPCPA a technology of Bidens tripartita extract obtained by 60 % ethanol extraction and standardized with flavonoids was developed. The aim of this study is to examine the regenerating properties of a hydrophilic gel based on Bidens tripartita extract obtained by 60 % ethanol extraction and compare them with properties of gels, based on Bidens tripartita extracts obtained by another extracting agents. Pharmacological studies were performed on 50 white outbred male mice with weigh of 18–20 g, which were kept on a standard diet in vivarium. The mice got a medical discission of 2 cm long with fixed edges. The mice were devided into 5 groups according to the substance applied. The substances were: 1 — a gel, based on Bidens tripartita extract obtained by 60 % ethanol extraction; 2 — a gel, based on Bidens tripartita extract obtained by 20 % ethanol extraction; 3 — a gel, based on Bidens tripartita extract obtained by water extraction; 4 — a comparator — an ointment “Calendula”; 5 — the control group. These substances were applied on mice’s discissions every day during a week until forming of scars. After that the scars were measured tensile tensiometrically. When comparing the groups of mice upon the mass of the weight at which the scar have broken, it was found out, that the heaviest weight is at the group of the gel, based on Bidens tripartita extract obtained by 60 % ethanol extraction. The weight is 425 g and thus the scars of the first group are the toughest. The other groups were compared with the first one. The order of decreasing of scar toughness is: group 3, group 2, group 4, group 5. The ratio of these groups relatively to the first group is –23,5 %, —33,0 %, —34,1 %, —38,9 % respectively. Statistically significant differences comparing with control group were not observed. Therefore, the gel, based on Bidens tripartita extract obtained by 60 % ethanol extraction has good regenerative properties and accelerates the process of scar-forming. The regenerative ability is regular and stable.