Carboxymethyl Cellulose Hydrogel Research Articles

PH-triggered Release of Boron and Thiamethoxam from Boric Acid Crosslinked Carboxymethyl Cellulose Hydrogel Based Formulations

ABSTRACTBoric acid crosslinked carboxymethyl cellulose hydrogels were synthesized to develop pH-triggered release formulations (pH-TRFs) of boron and thiamethoxam. The developed hydrogels showed pH sensitive water absorption capacity (16.75 to 110.80 g/g xerogel). Entrapment of thiamethoxam was done through an ex-situ loading technique. The boron and thiamethoxam release was studied in pH buffer solutions (4.0, 7.0 and 9.2). Release kinetics analysis using mathematical models showed fast release in high pH solution as compared to acidic pH. These pH-TRFs may find usefulness in selective release of nutrients and pesticides in plant rhizospheric zone of problem soils viz. acidic soils and alkaline soils.

Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials

The mechanical properties of soft materials used in the biomedical field play an important role on their performance. In the field of tissue engineering, it is known that cells sense the mechanical properties of their environment, however some materials, such as Sylard 184 PDMS (poly(dimethylsiloxane)), have failed to elicit such response. It was proposed that differences in the mechanical properties of such soft materials, at different scales, could account for these discrepancies. Indeed, the variation in the elastic moduli obtained for soft materials characterised at different scales can span several orders of magnitude. This called for a side-by-side comparison of the mechanical behaviour of soft materials at different scales. Here we use indentation, rheology and atomic force microscopy nanoidentation (using different tip geometries) to characterise the mechanical properties of PDMS, poly(acrylamide) (PAAm) and carboxymethyl cellulose (CMC) hydrogels at different length scales. Our results highlight the importance of surface adhesion and the resulting changes in contact area, and sample microstructural heterogeneity, in particular for the mechanical characterisation of ultra-soft substrates at the nano- to micro-scale.

Bioengineered carboxymethyl cellulose-doxorubicin prodrug hydrogels for topical chemotherapy of melanoma skin cancer

Melanoma is the most aggressive type of skin cancer with high rates of mortality. Despite encouraging advances demonstrated by anticancer drug carriers in recent years, developing ideal drug delivery systems to target tumor microenvironment by overcoming physiological barriers and chemotherapy side effects still remain intimidating challenges. Herein, we designed and developed a novel carbohydrate-based prodrug composed of carboxymethylcellulose (CMC) polymer bioconjugated with anticancer drug doxorubicin hydrochloride (DOX) by covalent amide bonds and crosslinked with citric acid for producing advanced hydrogels. The results demonstrated the effect of CMC hydrogel network structure with distinct degree of substitution of carboxymethyl groups of the cellulose backbone regarding to the process of bioconjugation and on tailoring the DOX release kinetics in vitro and the cytotoxicity towards melanoma cancer cells in vitro. To this end, an innovative platform was developed based on polysaccharide-drug hydrogels offering promising perspectives for skin disease applications associated with topical chemotherapy of melanoma.

Inhibition of Skin Wound Contraction by Nanofibrillar Cellulose Hydrogel.

Although wound contraction is an essential part of healing, excessive contraction can compromise healing through induction of scarring and fibrosis. This in turn leads to development of wound contractures that limit elasticity and function. Major research efforts have focused on development of novel therapeutic approaches to gain inhibitory control over wound contraction. Despite these efforts, the need for cost-effective, clinically feasible, and effective agents to inhibit wound contraction remains. In this study, the authors investigated the effect of nanofibrillar cellulose hydrogel on wound contraction both in vitro and in vivo. Two different porcine full-thickness wounds (8-mm punch-biopsy wounds and 4 × 4-cm wounds covered with a 1:3-meshed split-thickness skin graft) were treated with or without nanofibrillar cellulose or carboxymethylcellulose (Purilon hydrogel), which was used as a reference treatment. Wound contraction was observed macroscopically, and histologic sections were taken at 14-day follow-up. Nanofibrillar cellulose hydrogel inhibited 70 percent of punch-biopsy wound contraction, whereas the carboxymethylcellulose hydrogel was ineffective. Importantly, application of nanofibrillar cellulose on split-thickness skin grafts did not inhibit epithelialization of the interstices or cell migration from the graft. The authors' results, although preliminary, indicate a potential for nanofibrillar cellulose hydrogel as a novel material for controlling excessive wound contraction.

Biocompatible Magnetic Hydrogel Nanocomposite Based on Carboxymethylcellulose: Synthesis, Cell Culture Property and Drug Delivery

In this report, the synthesis of a novel biocompatible magnetic hydrogel nanocomposite based on carboxymethyl cellulose (CMC), as an eco-friend, biocompatible and green polysaccharide, is described via a facile one pot approach using magnetic iron oxide nanoparticles (MIONs) as a crosslinker. The structure of the prepared MION–CMC hydrogel nanocomposite was examined by various analytical techniques such as FTIR spectroscopy, TGA thermal analysis, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). The MIONs were generated in situ during the hydrogel formation with an average diameter size of 10 nm and a narrow size distribution. The sample was superparamagnetic with large saturation magnetization at room temperature. MION–CMC hydrogel nanocomposite showed a good ability for releasing of doxorubicin as an anticancer drug at pH 7 with case II (relaxational) transport mechanism. This outcome demonstrated that MION–CMC hydrogel nanocompositeis an attractive biocompatible candidate for widespread biomedical applications, particularly in controlled drug-targeting delivery.

Doxorubicin loaded carboxymethyl cellulose/graphene quantum dot nanocomposite hydrogel films as a potential anticancer drug delivery system

Creating anticancer properties in the hydrogel film could make it as a candidate for treating cancer tissues. In this work, a novel hydrogel nanocomposite films with anticancer properties were designed via incorporation of graphene quantum dot (GQD) as a nanoparticle into carboxymethyl cellulose (CMC) hydrogel and using doxorubicin (DOX) as drug model with broad-spectrum anticancer properties. Drug release studies carried out at two different pHs and the MTT assay was evaluated for DOX-loaded CMC/GQD nanocomposite hydrogel films against blood cancer cells (K562). The prepared nanocomposite hydrogel films were characterized using Fourier transform infrared (FT-IR), UV–Vis spectroscopy, scanning electron microscopy (SEM), permeability and mechanical properties. The prepared CMC/GQD nanocomposite hydrogel films showed an improvement in vitro swelling, degradation, water vapor permeability and pH-sensitive drug delivery properties along with not significant toxicity against blood cancer cells (K562). According to the obtained results, this nanocomposite hydrogel films can be proposed to use as an anticancer film and drug delivery system.

Shape-memory and self-healing functions of DNA-based carboxymethyl cellulose hydrogels driven by chemical or light triggers.

Photoresponsive nucleic acid-based carboxymethyl cellulose (CMC) hydrogels are synthesized, and their application as shape-memory and self-healing functional matrices are discussed. One system involves the preparation of a carboxymethyl cellulose hydrogel crosslinked by self-complementary nucleic acid duplexes and by photoresponsive trans-azobenzene/β-cyclodextrin (β-CD) supramolecular complexes. Photoisomerization of the trans-azobenzene to the cis-azobenzene results in a hydrogel exhibiting lower stiffness due to the separation of the azobenzene/β-CD bridging units. The hydrogel is switched between high and low stiffness states by the cyclic and reversible light-induced isomerization of the azobenzene units between the trans and cis states. The light-controlled stiffness properties of the hydrogel are used to develop a shape-memory hydrogel, where the duplex bridging units act as permanent memory in the quasi-liquid shapeless state of the hydrogel. A second system in the study is a carboxymethyl cellulose hydrogel crosslinked by the K+-stabilized G-quadruplex bridging units and by trans-azobenzene/β-CD complexes. The resulting hydrogel includes dual-trigger functionalities, where the trans-azobenzene/β-CD complexes can be reversibly formed and dissociated through the trans and cis photoisomerization of the azobenzene units, and the K+-stabilized G-quadruplexes can be reversibly dissociated and reformed in the presence of 18-crown-6-ether/K+-ions. The signal-responsive crosslinked hydrogel reveals controlled stiffness properties, where the hydrogel crosslinked by the trans-azobenzene/β-CD and K+-ion-stabilized G-quadruplex reveals high stiffness and the hydrogel crosslinked only by the K+-ion-stabilized G-quadruplexes or only by the trans-azobenzene/β-CD complexes reveals low stiffness properties. The controlled stiffness properties of the hydrogel are used to develop shape-memory hydrogels, where the trans-azobenzene/β-CD complexes or the K+-ion-stabilized G-quadruplexes act as permanent memories in the shapeless and quasi-liquid states of the hydrogels. In addition, the hydrogel that includes two types of stimuli-responsive crosslinking units is used as a self-healing matrix, where each of the triggers guides the self-healing processes.

Redox-triggered hydrogels revealing switchable stiffness properties and shape-memory functions

Metal-ion terpyridine-crosslinked acrylamide hydrogels or metal-ion-bridged carboxymethylcellulose hydrogels reveal redox-switchable stiffness and shape-memory properties.

Preparation and Characterization of Nanoporous Sodium Carboxymethyl Cellulose Hydrogel Beads

A series of nanoporous sodium carboxymethyl cellulose (NaCMC) hydrogel beads were prepared using FeCl3 ionic crosslinker by changing polymer and crosslinker percentages (%). Characteristics of the hydrogels were investigated by gel content, swelling test, degradation test, Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR), Scanning Electron Microscope/Energy Dispersive X-ray Analysis (SEM/EDX), and Atomic Force Microscopy (AFM). Swelling experiments were studied by changing time, temperature, and pH. The swelling percentage (S%) regularly decreased with increasing the amounts of polymer and crosslinker, in contrast with gel content results. NaCMC hydrogels were found to be sensitive to pH variations. The degradation test showed that hydrogels had good stability and their degradation period varied from 30 to 36 days. According to SEM analysis, NaCMC hydrogels had mostly nanoporous structure. The average granule and pore sizes of the least swollen NaCMC-12 hydrogel were found to be 13.1±0.3 nm and 82.1±3.2 nm. The elemental compositions of hydrogels were determined with EDX. The minimum average surface roughness (Ra) and root mean square roughness (Rms) parameters were found to be 15.7±1.9 nm and 20.3±2.2 nm for NaCMC-12 hydrogels by AFM. Due to their good morphologies, stabilities, and swelling behaviors, NaCMC hydrogels can be suitable for biomaterial applications.

Hydrogels Containing Antibiofilm and Antimicrobial Agents Beneficial for Biofilm-Associated Wound Infection: Formulation Characterizations and In vitro Study.

Bacterial biofilm which adheres onto wound surface is shown to be impervious to antibiotics and this in turn delays wound healing. Previous studies showed that antibiofilm agents such as xylitol and ethylenediaminetetraacetic acid (EDTA) prevent bacterial adherence onto surfaces. Formulation of a wound dressing containing antibiofilm agents may be a plausible strategy in breaking the biofilm on wound surfaces and at the same time increase the efficacy of the antibiotic. The purpose of this study was to develop hydrogel formulations containing antibiofilm agents along with antibiotic (gentamicin) for bacterial biofilm-associated wound infection. Sodium carboxymethyl cellulose (NaCMC) hydrogels loaded with antibiofilm agents and antibiotic were prepared. The hydrogels were characterized for their physical properties, rheology, Fourier transform infrared spectroscopy (FTIR), drug content uniformity, differential scanning calorimetry (DSC) and in vitro drug release study. The antibiofilm (Crystal Violet staining and XTT assay) and antibacterial performances of the hydrogels against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli were assessed in vitro. The formulated hydrogels showed adequate release of both antibiofilm agents (xylitol and EDTA). Both antimicrobial and antibiofilm tests showed promising results and demonstrated that the combination of xylitol, EDTA, and gentamicin had an additive effect against both Gram-positive and Gram-negative bacteria. In summary, NaCMC (sodium carboxymethyl cellulose) hydrogels containing the combination of antimicrobial and antibiofilm agents were successfully developed and this can be a new strategy in combating biofilm in wound infection which in turn accelerate wound healing.

Easily recoverable Cd x Zn1−x S-Gel photocatalyst with a tunable band structure for efficient and stable H2 production mediated by visible light

A photocatalyst consisting of a cellulose hydrogel loaded with Cd x Zn1−x S (Cd x Zn1−x S-Gel) was prepared, and its photocatalytic water splitting activity was studied. At room temperature, the Cd x Zn1−x S particles with an average size of about 3 nm were loaded in carboxymethyl cellulose hydrogel by an in situ chemical reaction. This synthesis method was simple and repeatable. Diffuse reflectance UV–vis results showed that it is possible to adjust the optical properties of the synthesized photocatalysts by adjusting their composition. Under visible light irradiation, the hydrogen production rate of the photocatalysts was greatly improved. In the absence of a co-catalyst, Cd0.2Zn0.8S-Gel obtained the highest hydrogen production rate of 1762.5 μmol g−1 h−1, which is almost 104 times of that of the pure CdS. Moreover, after four hydrogen production cycles, the hydrogen production rate did not decrease significantly, showing excellent stability. It is noteworthy that the Cd x Zn1−x S-Gel can easily be removed from the aqueous solution after hydrogen production. This is a new approach for manufacturing easy recoverable visible-light-driven photocatalysts, which is vital for hydrogen production through a “green” process.

In Situ Synthesis of Silver Nanoparticles in a Hydrogel of Carboxymethyl Cellulose with Phthalated-Cashew Gum as a Promising Antibacterial and Healing Agent.

Silver nanoparticles have been shown to possess considerable antibacterial activity, but in vivo applications have been limited due to the inherent, but low, toxicity of silver. On the other hand, silver nanoparticles could provide cutaneous protection against infection, due to their ability to liberate silver ions via a slow release mechanism, and their broad-spectrum antimicrobial action. Thus, in this work, we describe the development of a carboxymethyl cellulose-based hydrogel containing silver nanoparticles. The nanoparticles were prepared in the hydrogel in situ, utilizing two variants of cashew gum as a capping agent, and sodium borohydride as the reducing agent. This gum is non-toxic and comes from a renewable natural source. The particles and gel were thoroughly characterized through using rheological measurements, UV-vis spectroscopy, nanoparticles tracking analysis, and transmission electron microscopy analysis (TEM). Antibacterial tests were carried out, confirming antimicrobial action of the silver nanoparticle-loaded gels. Furthermore, rat wound-healing models were used and demonstrated that the gels exhibited improved wound healing when compared to the base hydrogel as a control. Thus, these gels are proposed as excellent candidates for use as wound-healing treatments.

Eco-friendly and biocompatible cross-linked carboxymethylcellulose hydrogels as adsorbents for the removal of organic dye pollutants for environmental applications

ABSTRACTIn this study, new eco-friendly hydrogel adsorbents were synthesized based on carboxymethylcellulose (CMC, degree of substitution [DS] = 0.7) chemically cross-linked with citric acid (CA) using a green process in aqueous solution and applied for the adsorption of methylene blue (MB). Spectroscopic analyses demonstrated the mechanism of cross-linking through the reaction of hydroxyl functional groups from CMC with CA. These CMC hydrogels showed very distinct morphological features dependent on the extension of cross-linking and their nanomechanical properties were drastically increased by approximately 300% after cross-linking with 20% CA (e.g. elastic moduli from 80 ± 15 to 270 ± 50 MPa). Moreover, they were biocompatible using an in vitro cell viability assay in contact with human osteosarcoma-derived cells (SAOS) for 24 h. These CMC-based hydrogels exhibited adsorption efficiency above 90% (24 h) and maximum removal capacity of MB from 5 to 25 mg g−1 depending on the dye concentration (from 100 to 500 mg L−1), which was used as the model cationic organic pollutant. The adsorption of process of MB was well-fit to the pseudo-second-order kinetics model. The desorption of MB by immersion in KCl solution (3 mol L−1, 24 h) showed a typical recovery efficiency of over 60% with conceivable reuse of these CMC-based hydrogels. Conversely, CMC hydrogels repelled methyl orange dye used as model anionic pollutant, proving the mechanism of adsorption by the formation of charged polyelectrolyte/dye complexes.

Superabsorbent crosslinked carboxymethyl cellulose-PEG hydrogels for potential wound dressing applications

This study focused on the synthesis and comprehensive characterization of environmentally friendly hydrogel membranes based on carboxymethyl cellulose (CMC) for wound dressing and skin repair substitutes. These new CMC hydrogels were prepared with two degrees of functionalization (DS=0.77 and 1.22) and chemically crosslinked with citric acid (CA) for tuning their properties. Additionally, CMC-based hybrids were prepared by blending with polyethylene glycol (PEG, 10wt.%). The results demonstrated that superabsorbent hydrogels (SAP) were produced with swelling degree typically ranging from 100% to 5000%, which was significantly dependent on the concentration of CA crosslinker and the addition of PEG as network modifier. The spectroscopical characterizations indicated that the mechanism of CA crosslinking was mostly associated with the chemical reaction with CMC hydroxyl groups and that PEG played an important role on the formation of a hybrid polymeric network. These hydrogels presented very distinct morphological features depended on the degree of crosslinking and the surface nanomechanical properties (e.g., elastic moduli) were drastically affected (from approximately 0.08GPa to 2.0GPa) due to the formation of CMC-PEG hybrid nanostructures. These CMC-based hydrogels were cytocompatible considering the in vitro cell viability responses of over 95% towards human embryonic kidney cells (HEK293T) used as model cell line.

Optimization of carboxymethyl cellulose hydrogels beads generated by an anionic surfactant micelle templating for cationic dye uptake: Swelling, sorption and reusability studies

Carboxymethyl cellulose hydrogels beads were synthesized by ionotropic gelation in Al3+ solutions with or without sodium n-dodecyl sulfate (SDS) surfactant as a pore-forming micelle templating and NaCl as porogen agent. FTIR spectroscopy and EDX analysis evidenced the formation of hydrogels. The morphology observed by Scanning Electron Microscopy (SEM) showed rough porous surfaces. The swelling properties in various media were studied. The addition of SDS optimized the capacity and initial rate of swelling. The porous beads were assessed as adsorbents to remove methylene blue (MB) dye from aqueous solutions by varying parameters: pH medium, adsorbent dose, initial MB concentration, and contact time. The kinetics obeyed well to pseudo-second-order model and the isotherms data followed Langmuir model with a maximum of adsorption capacity of 82mgg−1. Thermodynamic studies showed spontaneous and endothermic nature of the process. From reusability study, all beads are well regenerated and displayed unexpected increase in removal efficiency compared to starting ones, with an optimal capacity of 350mgg−1. This fact is due to dye entrapped in the desorbed beads that favored subsequent dye access to internal sites. In view of effectiveness of adsorption and regeneration, the optimized hydrogels are promising as low cost dyes adsorbents.

Carboxymethylcellulose hydrogels cross-linked with magnetite nanoparticles for the removal of organic and inorganic pollutants from water

Magnetic hydrogels based on carboxymethylcellulose (CMC) were prepared using magnetite (Fe3O4) nanoparticles (NPs) functionalized with 3-aminopropyltrimethoxysilane (APTMS) as cross-linker. In this hybrid organic-inorganic hydrogel, magnetite NPs are covalently bonded to the polymer chains. The CMC-Fe3O4 hydrogel was characterized by means of Attenuated Total Reflection Fourier Transform Infra Red Spectroscopy (ATR-FTIR) and Field Emission Scanning Electron Microscopy (FESEM). The equilibrium swelling degree of the CMC-Fe3O4 hydrogels was measured in deionized water as well as in artificial and natural sea water. Bioassays were performed in order to assess the ecosafety of these magnetic hydrogels by testing any potential effect on the growth rate of marine microalgae Dunaliella tertiolecta. The capability of the CMC-Fe3O4 hydrogel to adsorb organic and inorganic pollutants was tested on solutions of methylene blue (MB) and CdCl2 in deionized and sea water as model systems. The CMC-Fe3O4 hydrogel is capable to adsorb both MB and Cd(II) in deionized water. On the other hand, the adsorption capacity is strongly reduced in sea water, due to the ions in solution which decrease the electrostatic attraction between the carboxylate groups of CMC and the positive charge of MB and Cd(II).

Chestnut Honey Impregnated Carboxymethyl Cellulose Hydrogel for Diabetic Ulcer Healing.

Honey-based wound dressings have attracted a lot of attention from modern scientists owing to their anti-inflammatory and antibacterial effects without antibiotic resistance. Such dressings also promote moist wound healing, and have been considered natural, abundant, and cheap materials for folk marketing. This study investigated the various behaviors and characteristics of chestnut honey-impregnated carboxymethyl cellulose sodium hydrogel paste (CH–CMC) as a therapeutic dressing, such as its moist retention, antibacterial activity for inhibiting the growth of Staphylococcus aureus and Escherichia coli, and the rate of wound healing in db/db mice. The results provide good evidence, suggesting that CH–CMC has potential as a competitive candidate for diabetic ulcer wound healing.

An intratumoral injectable, electrostatic, cross-linkable curcumin depot and synergistic enhancement of anticancer activity

In this study, we describe an intratumoral injectable, electrostatic, cross-linkable curcumin (Cur) drug depot to enhance anticancer activity. The key concept in this work was the preparation of an electrostatic, cross-linked carboxymethyl cellulose (CMC) and chitosan (CHI) hydrogel containing Cur-loaded microcapsules (Cur-M). The CMC and CHI solutions existed as a liquid before mixing and formed a CMC and CHI (CCH) hydrogel as a drug depot after mixing via electrostatic interactions between the anionic CMC and cationic CHI. Compared with the individual CMC and CHI solutions, the electrostatic, cross-linked CCH depot persisted in vivo for an extended period. The prepared Cur-M was easily mixed with the CMC and CHI solutions. Cur-M/CMC and Cur-;M/CHI solutions easily formed Cur-M-loaded CCH depots after simple mixing. The in vitro and in vivo Cur-M-loaded CCH depot was designed with Cur-M dispersed inside an outer shell of electrostatically cross-linked CCH. The Cur-M-loaded CCH depot produced greater inhibition of tumor growth than did Cur-M, whereas single and repeated injections of free Cur had the weakest inhibitory effects. The results of this study indicate that the electrostatic, cross-linked, Cur-M-loaded CCH depot described in this study can synergistically enhance anticancer activity in chemotherapeutic delivery systems.

Strength and sorption capacity modulation of carboxymethylcellulose hydrogels in presence of ester-bonded gemini surfactants

The present work describes strength modulation of carboxymethylcellulose (CMC) hydrogel on addition of biodegradable diester-bonded gemini surfactants. These CMC-surfactant hybrid hydrogels were prepared using carboxymethylcellulose sodium salt and gemini surfactants in urea solution. The structure and morphology of the synthesized hybrid hydrogels were characterised using FT-IR spectroscopy and scanning electron microscopy. The gelling behaviour of synthesized hybrid hydrogels were investigated in distilled water in presence of different salts and differing pH's. The swelling capacity of these hydrogels were found to decrease with an increase in the charge of the metal cation ions (Al3+<Ca2+<Na+). The rheometric observations reveal an increase in the elastic modulus (G) of the CMC hydrogel on addition of surfactant, indicating an increase in the mechanical strength of the hydrogel. However, at higher surfactant concentrations the G value progressively decreased. The effect of added surfactants on gelation temperature of CMC was investigated through oscillating temperature sweep. The gelation temperature of CMC-urea+16-E2-16 surfactant was found to be higher than that of CMC-urea+14-E2-14 gemini surfactant. The encapsulation capacity of synthesized CMC-urea+surfactant hybrid hydrogel systems were explored for sorption capacity of various hazardous polynuclear aromatic hydrocarbons (PAHs) from their aqueous solutions. The observed results suggest that sorption of PAHs using CMC-urea+surfactant hydrogel systems can be a valuable approach towards safer remediation of groundwater contaminated by toxic organic compounds.

A Dual-Enzyme Hydrogen Peroxide Generation Machinery in Hydrogels Supports Antimicrobial Wound Treatment

The aging population and accompanying diseases like diabetes resulted in an increased occurrence of chronic wounds. Topical wound treatment with antimicrobial agents to inhibit bacterial invasion and promote wound healing is often associated with difficulties. Here, we investigated the potential of succinyl chitosan (SC)-carboxymethyl cellulose (CMC) hydrogels which constantly release clinically relevant levels of hydrogen peroxide (H2O2). CMC hydrogel matrix was in situ converted by limited hydrolysis by a cellulase into substrates accepted by cellobiose dehydrogenase (CDH) for continuous production of H2O2 (30 μM over 24 h). This dual-enzyme catalyzed in situ H2O2 generation system proved its antimicrobial activity in a zone of inhibition (ZOI) assay best simulating the application as wound dressing and was found to be biocompatible toward mouse fibroblasts (95% viability). The hydrogels were thoroughly characterized regarding their rheological properties indicating fast gel formation (<3 min) and moderate cross-linking (1.5% strain, G' = 10 Pa). Cooling (fridge conditions) was found to be the simple on/off switch of the enzymatic machinery which is of great importance regarding storage and applicability of the bioactive hydrogel. This robust and bioactive antimicrobial hydrogel system overcomes dosing issues of common topical wound treatments and constitutes a promising wound healing approach for the future.