Hydration Level Research Articles

Contactless Drink Intake Monitoring Using Depth Data

It is important for humans to remain hydrated, particularly for older adults who are at a greater risk of dehydration and may forget to drink. Monitoring liquid intake and getting reminders to drink throughout the day is a useful solution to increase hydration levels. The objective of this paper is to automatically detect drink events from multiple containers in a simulated home environment using a vision-based approach. The proposed work compares the use of depth and RGB (red, green, blue) cameras for this task. In this paper, we compared 2D and 3D Convolutional Neural Networks (CNN) using RGB and depth cameras. We collected data from nine participants performing drinking, eating and other Activities of Daily Living (ADL) in a simulated home environment. We found that for the 3D models, the RGB and depth camera inputs provided very similar F1-scores for both 10-Fold (94.3% vs 93.9%, respectively) and Leave-One-Subject-Out (LOSO) cross validation (84.2% vs 86.2%, respectively). This is a promising result as depth cameras also mitigate the challenges to privacy of RGB-based models. The 3D CNN models outperformed the 2D models, thereby creating a more robust system. Depth cameras are a useful alternative to RGB cameras with equal performance in identifying drinking events.

Rosa gallica and its active compound, cyanidin-3,5-O-diglucoside, improve skin hydration via the GLK signaling pathway.

Rosa gallica has been previously reported to display anti-inflammatory, anti-oxidative, and anti-skin wrinkle activities. However, the effect of Rosa gallica on skin hydration and its active components are largely unknown. Herein, we aimed to investigate the skin hydration effect of rose petal extract (RPE) in humans and elucidate the underlying molecular mechanism. A double-blinded clinical study was performed to investigate the effect of RPE on skin hydration. Stratum corneum moisture analysis demonstrated that RPE treatment significantly improved hydration levels in human skin. Furthermore, HAS2 and hyaluronic acid levels were notably increased by RPE in keratinocytes and 3D human skin equivalent model. By comparing the modulatory effect on HAS2 expression, cyanidin-3,5-O-diglucoside (CDG) was identified as the most potent compound in RPE likely responsible for skin hydration. The kinase activity of GLK, an upstream regulator of MAPK signaling, was increased by CDG in a dose-dependent manner. Importantly, silencing GLK reversed CDG-mediated HAS2 upregulation, further supporting the involvement of GLK in the CDG-mediated effects. Binding of CDG to GLK was confirmed by pull-down assay and computer modeling. These findings suggest that RPE and its active component CDG increases skin hydration by upregulating HAS2 expression through modulating the GLK-MAP2K-MAPK signaling pathway.

Water mobility and microstructure of gluten network during dough mixing using TD NMR

This work aimed to establish the relationships between flour components, dough behaviour and changes in water distribution at mixing. TD NMR was used to track water distribution in dough during mixing for different mixing times and hydration levels. Four commercial wheat flours with distinct characteristics were expressly selected to exhibit various dough behaviours at mixing. TD NMR measurements of mixed dough samples revealed four to five water mobility domains depending on the flour type and the mixing modality. A classification tree procedure was used to identify characteristic patterns of water mobility in dough, called hydration states (HS). The HS changes with experimental conditions are highly dependent on flour characteristics, and HS were assigned to physical/chemical changes in the gluten network during dough formation. This study proposes an interpretation of the water distribution in dough based on gluten network development. This will help to adapt the mixing process to the flour characteristics.

Proton Transfers to DNA in Native Electrospray Ionization Mass Spectrometry: A Quantum Mechanics/Molecular Mechanics Study.

Native electrospray ionization-ion mobility mass spectrometry (N-ESI/IM-MS) is a powerful approach for low-resolution structural studies of DNAs in the free state and in complex with ligands. Solvent vaporization is coupled with proton transfers from ammonium ions to the DNA, resulting in a reduction of the DNA charge. Here we provide insight into these processes by classical molecular dynamics and quantum mechanics/molecular mechanics free energy calculations on the d(GpCpGpApApGpC) heptamer, for which a wealth of experiments is available. Our multiscale simulations, consistent with experimental data, reveal a highly complex scenario. The proton either sits on one of the molecules or is fully delocalized on both, depending on the level of hydration of the analytes and the size of the droplets formed during the electrospray experiments. This work complements our previous study of the intramolecular proton transfer on the same heptamer occurring after the processes studied here, and together, they provide a first molecular view of proton transfer in N-ESI/IM-MS.

The Effects of Dehydration Resulting from Basic Endurance Training (End-1) on Performance and Examination of Body Hydration Level on Swimmers

The purpose of this study, basic endurance training in swimming is the evaluation of the resulting dehydration and its effects on performance. 14 male swimmers from Pamukkale University Swimming Sports Clup team participated voluntarily in this study. 7 athletes took liquid supplement and 7 athletes continued to practice without liquid supplement and the same protocol were repeated after 48hours. Tanita measurements, urine specific gravity, heart rate, blood pressure, body temperature, swimming performance (100m and 400m) measurements was applied. T-test was used for the difference between the pre-test and post-test values in the group, and t-test analysis was used for the differences between the groups in the independent groups. There was no statistically significant difference between in the pre-test values of the swimmers with and without liquid supplementation (p>0.05). There was a statistically significant difference in the urine density values between in the post-test values of the swimmers with and without liquid supplementation (p

New Solid Forms of Nitrofurantoin and 4-Aminopyridine Salt: Influence of Salt Hydration Level on Crystal Packing and Physicochemical Properties.

The crystallization of the poorly soluble drug nitrofurantoin (NFT) with 4-aminopyridine (4AmPy) resulted in three multicomponent solid forms with different hydration levels: anhydrous salt [NFT+4AmPy] (1:1), salt monohydrate [NFT+4AmPy+H2O] (1:1:1), and salt tetrahydrate [NFT+4AmPy+H2O] (1:1:4). Each salt was selectively prepared by liquid-assisted grinding in the presence of acetonitrile or ethanol/water mixture at a specific composition. The NFT hydrated salts were characterized using single crystal X-ray diffraction. The [NFT+4AmPy+H2O] salt (1:1:1) crystallized as an isolated site hydrate, while the [NFT+4AmPy+H2O] salt (1:1:4) crystallized as a channel hydrate. The dehydration processes of the NFT salt hydrates were investigated using differential scanning calorimetry and thermogravimetric analysis. A powder dissolution experiment was carried out for all NFT multicomponent solid forms in pH 7.4 phosphate buffer solution at 37 °C.

Experimental Investigation of Electro-Mechanical Behavior of Silver-Coated Teflon Fabric-Reinforced Nafion Ionic Polymer Metal Composite with Carbon Nanotubes and Graphene Nanoparticles.

Ionic Polymer Metal Composites (IPMCs) are in high demand owing to the ongoing advancements in technology for various applications. New fabrication techniques and a quick retort towards the applied load are the significant reasons for considering IPMCs in smart devices. Here, a Teflon fabric-reinforced Nafion (TFRN) membrane is used to create an IPMC. The materials employed as electrodes are silver and nanofillers. The basement membrane, Nafion 438 (N-438), is sandwiched between the electrodes using a chemical decomposition technique. Subsequently, the electromechanical properties (actuation) of the membrane are tested. The micro and molecular structure of the IPMC membrane coated with Silver (Ag), Ag-Carbon nanotubes (CNTs), and Ag-Graphene nanoparticles samples are examined with the help of SEM and X-ray diffraction (XRD). The membrane scratch test is carried out to evaluate the abrasion and wear resistance of the membrane. The lowest coefficient of friction is shown by N438 + Ag + Graphene (0.05), which increased by 300% when compared to a pure N438 membrane. The hydration and tip deflection test were also performed to understand the water uptake percentage. At 90 °C, the highest water uptake was observed for N438 + Ag + Graphene (0.05), which decreased by 60, 42, 23, 14 and 26% when compared to N438, N438 + Ag, N438 + Ag + CNT (0.01), N438 + Ag + CNT (0.05) and N438 + Ag + Graphene (0.01), respectively. A proportional relationship between hydration level and tip deflection is observed and the highest bending performance is observed for the N438 + Ag + Graphene (0.05) membrane.

Morphological Effect of Side Chain Length in Sulfonated Poly(arylene ether sulfone)s Polymer Electrolyte Membranes via Molecular Dynamics Simulation.

With the recognition of the multiple advantages of sulfonated hydrocarbon-based polymers that possess high chemical and mechanical stability with significant low cost, we employed molecular dynamics simulation to explore the morphological effects of side chain length in sulfonated polystyrene grafted poly(arylene ether sulfone)s (SPAES) proton exchange membranes. The calculated diffusion coefficients of hydronium ions (H3O+) are in range of 0.61-1.15 × 10-7 cm2/s, smaller than that of water molecules, due to the electrical attraction between the oppositely charged sulfonate group and H3O+. The investigation into the radial distribution functions suggests that phase segregation in the SPAES membrane is more probable with longer side chains. As the hydration level of the membranes in this study is relatively low (λ = 3), longer side chains correspond to more water molecules in the amorphous cell, which provides better solvent effects for the distribution of sulfonated side chains. The coordination number of water molecules and hydronium ions around the sulfonate group increases from 1.67 to 2.40 and from 2.45 to 5.66, respectively, with the increase in the side chain length. A significant proportion of the hydronium ions appear to be in bridging configurations coordinated by multiple sulfonate groups. The microscopic conformation of the SPAES membrane is basically unaffected by temperature during the evaluated temperature range. Thus, it can be revealed that the side chain length plays a key role in the configuration of the polymer chain and would contribute to the formation of the microphase separation morphology, which profits proton transport in the hydrophilic domains.

Vacuum and Electromagnetic Fields Treatment to Regenerate a Diffuse Mature Facial Scar Caused by Sulfuric Acid Assault.

Acid attacks are on the rise, and they cause extensive and deep burns, especially on the face. The treatments used to improve the aesthetic, functional and social impact of non-acid scars do not always prove useful for acid scars. This article reports the case of a woman with an extended, mature, acid facial scar, caused by sulfuric acid assault, treated with a recent new procedure that combines the application of vacuum and electromagnetic fields. Before and after the treatment, the aesthetic appearance, and motor function of the face and neck were evaluated, as well as the level of hydration, the amount of sebum, the elasticity, and the pH of the skin. The improvements highlighted after the treatment of the aesthetic and functional characteristics of the face and neck, and of the physical parameters of the skin seemed to indicate that this particular treatment induces tissue regeneration, even in the nerve component. However, it is evident that the rehabilitation pathways of facial wounds and scars must be personalized, and must include continuous psychological support for the patient.

Two Forms of Ice Identified in Mars-like Clay Using Neutron Spectroscopy

The capacity of clay minerals to store large amounts of water is utilized in a number of industrial and environmental applications on Earth, for example, as components in geosynthetic clay liners in landfills or ingredients in water-based drilling fluids, and could prove important on Mars to identify future human landing sites where water could be harvested. The subzero behavior of water interacting within the interlayer space of clay minerals is of particular interest in most applications but remains poorly understood. To better understand the hydrothermal mechanism by which water ice bonds and separates from clay interlayers, we have utilized neutron spectroscopy, spectral analysis, and phonon band assignment. The inelastic neutron scattering from sodium montmorillonite, hydrated at 24, 73, and 166% water content, as well as an oven-dried sample, were measured to assess the vibrational density of states. The water contents studied provide a range of pore dimensions within clay gels that have varying degrees of confinement. The type of ice formed from water held in larger intra- and interparticle pores differs substantially from that confined within the interlayer (pseudo-two-layer hydrate), and the differences vary with hydration level. Spectral subtraction over an energy transfer range 50 < E < 550 cm–1 (8 < E < 70 meV) produces clearly two different forms of ice: hexagonal and cubic in the two wetter samples. A form of interfacial ice, presumably of a lower density, is observed in the vibrational density of states spectrum of the sample hydrated to a pseudo-two-layer hydrate (ie 24% gravimetric water content (GWC), 10 H2O/Na+). No hexagonal or cubic ice is observed in this sample. The four vibrational modes within the translation band of hexagonal ice are apparent within the sample hydrated to 166% gravimetric water content, in which pores greater than 20 nm are largely water-filled. By considering hydrogen bonding of the water to the clay surface, our data indicate an increase in the strength of the H-bond due to a shorter distance to the hydroxyl. We attribute this decrease to the pores in the clay generating a localized negative pressure or “suction” effect, thus attracting the water.

Cervical Tissue Hydration Level Monitoring by a Resonant Microwave Coaxial Probe

Cervical tissue hydration level is one of the most important parameters to monitor in the early diagnosis of preterm birth. Electrical-impedance-spectroscopy-based techniques are often used, but they suffer from limited accuracy. Open microwave coaxial probes have been widely used as a broadband dielectric characterization technique for human tissue samples due to their versatility, but with limited accuracy due to their nonresonant nature. In this work, a resonant microwave open coaxial probe with multiple harmonic resonances is proposed as a sensing platform for tissue-hydration-level monitoring. The mechanical design was analyzed and verified by finite-element full 3D electromagnetic simulation and experiments. Dominant sources of errors and the ways to mitigate them were discussed. In vitro experiments were carried out on human cervix samples to verify the precision and accuracy by comparing the results to a commercial skin-hydration sensor. The proposed sensor shows mean fractional frequency shift of (3.3 ± 0.3) × 10-4 per unit % over the entire data. This translates into an absolute frequency shift (ΔfN) of 252 ± 23 kHz/%, 455 ± 41 kHz/%, and 647 ± 57 kHz/% at second, fourth, and sixth harmonic resonance, respectively.

Innovative Insight into O2/N2 Permeation Behavior through an Ionomer Film in Cathode Catalyst Layers of Polymer Electrolyte Membrane Fuel Cells.

It is crucial to clarify the permeation behavior of O2 through the ionomer film for enhancing local O2 transport in cathodes of fuel cells. However, all existing studies mainly deal with pure O2 rather than air. Herein, the permeation behavior of the O2/N2 mixture through the ionomer film has been well explored in view of molecular bond length variations by molecular dynamics simulations. The bond lengths for O2 and N2 are shortened under a low hydration level when permeating through a dense layer with small free voids while no obvious change occurs at higher hydration. In the bulk ionomer region, O2 molecules residing in water domains are energetically unstable because the bond lengths deviate far from the equilibrium length; thus, O2 diffuses through the interfacial or hydrophobic regions. N2 molecules show similar properties with O2. This study provides a novel perspective on the permeation behavior of O2 and N2 through the ionomer film, which definitely benefits enhancing local O2 transport.

Effectiveness Test of White Skin Extract Cream from Red Watermelon (Citrullus Lanatus) on Increasing Elasticity, Sebum and Hydration in White Mice (Mus Musculus) Skin

Skin moisturizing gel can provide a moisturizing effect on the skin. One of the natural ingredients that can be made into gel preparations is the white peel of watermelon (Citrullus Lanatus) because it contains a lot of citrulline, vitamin A, vitamin B2, vitamin B6, vitamin E, vitamin C, and protein, beta-carotene, lycopene, and water. The purpose of this study was to determine the activity of the ethanol extract of watermelon rind on increasing elasticity, sebum, and hydration in the skin of male white mice. This study used the Pretest-Posttest Control Group Design method. The stages of the research were sample collection, extraction, formulation of various concentrations of moisturizing gel preparations (0, 2.5%, 5%, and 10%). Tests were carried out on the skin of male mice for 4 weeks by measuring levels of elasticity, sebum, and hydration. The results of the phytochemical screening extracts contained flavonoids, saponins, steroids, triterpenoids, and glycosides. The evaluation results showed that all formulas met good preparation parameters and did not irritate the skin. The measurement results in the fourth week of each cream preparation group showed an increase in the levels of elasticity, sebum and hydration and differed significantly from the blank group (p &lt;0.05) and the highest increase occurred in cream with watermelon rind extract concentration 10 %. The results of this study indicate that the cream preparation of ethanol extract of watermelon rind can increase the levels of elasticity, sebum, and hydration of the skin.

Test Of the Effectiveness of Tomato (Lycopersicum Esculentum Mill) Fruit Extract Cream on Increasing Elasticity, Sebum and Hydration in White Mouse Skin (Mus Musculus)

The skin is the outermost organ that acts as a protector from the external environment both in the ligaments, muscles, and internal organs from exposure to direct sunlight (ultraviolet light) and dehydration, also functions as a sense of taste and touch as well as a defense against pressure and infection. The imbalance between oxidants and antioxidants is influenced by external factors and internal factors that will affect wrinkling and aging of the skin. Tomatoes contain substances such as citrulline, vitamin A, vitamin B2, vitamin B6, vitamin E, vitamin C, and protein, beta-carotene, lycopene, and water. The purpose of this study was to determine the activity of tomato fruit ethanol extract to increase elasticity, sebum and hydration in the skin of male white mice. Cream preparations of tomato fruit ethanol extract were formulated with concentrations of 2.5%, 5%, and 10%. The test was carried out on the skin of male mice for 4 weeks by measuring the levels of elasticity, sebum and hydration. The results of phytochemical screening of extracts contained flavonoids, saponins, steroids, triterpenoids, and glycosides. The measurement results in the fourth week of each group of cream preparations showed an increase in the levels of elasticity, sebum and hydration and was significantly different from the blank group (p&lt;0.05) and the highest increase occurred in cream with 10% tomato extract concentration. The results of this study indicate that the preparation of tomato fruit ethanol extract cream can increase the elasticity, sebum and hydration levels of the skin.

Differential Water Deficit in Leaves Is a Principal Factor Modifying Barley Response to Drought Stress

In response to environmental stress, plants activate complex signalling, including being dependent on reactive oxygen-nitrogen-sulphur species. One of the key abiotic stresses is drought. As a result of drought, changes in the level of hydration of the plant occur, which obviously entails various metabolic alternations. The primary aim of this study was to determine the relationship between the response of barley to drought and the intensity of stress, therefore investigations were performed under various levels of water saturation deficit (WSD) in leaves at 15%, 30%, and 50%. In barley subjected to drought, most significant changes occurred under a slight dehydration level at 15%. It was observed that the gene expression of 9-cis-epoxycarotenoid dioxygenases, enzymes involved in ABA biosynthesis, increased significantly, and led to a higher concentration of ABA. This was most likely the result of an increase in the gene expression and enzyme activity of L-cysteine desulfhydrase, which is responsible for H2S synthesis. Our results suggest that the differential water deficit in leaves underlies the activation of an appropriate defence, with ABA metabolism at the centre of these processes. Furthermore, at 15% WSD, a dominant contribution of H2O2-dependent signalling was noted, but at 30% and 50% WSD, significant NO-dependent signalling occurred.

Molecular Hydration Tunes the Cation–π Interaction Strength in Aqueous Solution

AbstractCation–π interactions are considered to be among the most important strong noncovalent interactions in aqueous solutions and as regulators of various biological phenomena, including biological adhesion, signal transduction, and liquid–liquid phase separation (LLPS). Despite their significant roles, cation–π interactions in aqueous environments are not systematically and experimentally understood. Here, we directly experimentally examined cation–π interactions between cationic and aromatic moieties common to biological systems using a surface forces apparatus (SFA) augmented by computational methods. Specifically, we chose cationic and aromatic moieties with different levels of hydration and observed how the molecular hydration of these moieties affects the formation and strengths of cation–π bonds. To date, the charge densities of the interacting moieties are primarily considered to modulate the interaction strength; however, it is experimentally and computationally revealed that the hydration strengths (or hydrophobic properties) of the interacting moieties are more important for controlling in underwater interactions.

The Influence of Carboxytherapy on Scar Reduction.

Although it is not a new method, a carboxytherapy, which is based on intradermal or subcutaneous administration of controlled doses of CO2, is gaining more and more recognition among aesthetic medicine doctors, dermatologists and cosmetologists around the world. The consequence of applying carbon dioxide directly into tissues is associated with an immediate expansion of blood vessels, improvement of local blood supply, and, thus, tissue metabolism. It does also support natural regenerative processes. Oxygen and growth factors released from blood, within the area which undergoes treatment, stimulate fibroblasts to produce collagen and formation of new blood vessels also known as neovascularization. In addition to biochemical mechanisms, CO2 injection into the dermis or subcutaneous tissue leads to a mechanical effect exerted by pressure and flow of CO2 which is injected. It is of particular importance in scar treatments. Twelve mature scars were subjected to the carboxytherapy which was performed in people aged 23-45 years. A small amount of heated, medical CO2 was injected till the moment a scar turns white. The applied flow rate equalled 100 mL/min (cc/min). Before and after a series of four treatments, the level of hydration, elasticity and colour of the skin were measured. A structured-light 3D scanner was used to determine an exact morphology of the examined scars. The 3D scanning device is seen as a sensitive and precise method of qualitative and quantitative assessment of a morphology of scars. The results of the measurements performed showed a reduction in the surface of the analyzed changes, as well as proved the ability of CO2 to rebuild collagen fibres. The decrease in value of parameters, which have been obtained thanks to the kutometric examination, indicates softening and loosening of connective tissue. It does confirm the effectiveness of carboxytherapy.

Surprising Solid-State ESIPT Emission from Apparently Ordinary Salicyliden Glycinates Schiff Bases

Excited-State Intramolecular Photon Transfer (ESIPT) is known for the geometry-related phenolic and imine groups. The Schiff bases formed upon condensation of salicyl aldehyde and glycine led to the formation of ESIPT models. A series of alkali metal salicyliden glycinates were analyzed by X-ray diffraction of their monocrystals and spectroscopy measurements. The X-ray analysis revealed varied hydration levels between the salts. They adapted trans geometry on the imine groups and mostly anticlinal conformation with the neighboring atoms, which is different from the other structurally-related compounds in literature. Fluorescence of these compounds was found for the crystalline forms only. Protonation of the imine nitrogen atom and further proton distribution was consistent with the ESIPT theory, which also explained the observed fluorescence with the highest Stokes shift of 10,181 cm-1 and 10.1% of fluorescence quantum yield for the sodium salt.

Characterisation of likelihood of gas hydrates occurrence in the South China Sea based on Bonferroni mean-based TOPSIS and fuzzy set theory

The efficiency of gas hydrate production depends on the success of gas exploration and occurrence evaluation. The existing evaluation models are generally univariate and only applicable to certain geological settings. This study presents a holistic approach to evaluate the likelihood of gas hydrate occurrence by supplying an index for mapping gas hydrate levels with depth. The approach integrates a generalised TOPSIS method with the fuzzy set theory. An expedition of gas hydrate conducted in the Shenhu area of the South China Sea was adopted as a case study to assess the reliability of the proposed index. As a multivariate model, the proposed approach enables the capture of non-linearity associated with gas hydrates in its entirety. The magnitude of the strength of the influential factor varies substantially from one site to another across the Shenhu area. The results also show that no site achieves the highest likelihood ‘Level V’. These results are consistent with the gas saturation values obtained using Archie’s relationship. For example, at SH4 and SH7, the values of the likelihood index are the highest between 170–185 m and 150–165 m, respectively, and the observed saturation at these locations varies from 20% (SH4) to 43% (SH7). The proposed likelihood index yields a prominent ability to quantify the level of occurrence of gas hydrates with depth at different sites. It appears to be an efficient multicriteria system bound to improve the management of the gas production trial stage.

257 Clinical skin moisturizing property of the AVENE thermal spring water is associated with water capture and higher lipid organization in the stratum corneum

One of the main functions of the skin is to provide an optimal water level for normal physiological function and maintain its biophysical properties, including elasticity. The aim of this study was to demonstrate clinically the moisturizing effect of AVENE thermal spring water (TSW) and explain the mechanism of action involved, using biophysical analysis of the stratum corneum (SC) approaches. Skin electric capacitance was used to assess the hydration level of the skin outermost layers of 24 volunteers, using corneometry, after 6 daily applications of AVENE TSW for 7 days. The persistence of this effect was analyzed for 20 hours after the last application. In addition, ex vivo raman spectroscopy was performed on isolated SC. Hydration level and barrier function were directly monitored by following the different vibrational contributions of water and lipids in the SC Raman spectra. We clinically observed a significant improvement in hydration of the upper layers of the epidermis which persisted for 20 hours after the last application compared to baseline and to untreated area. Indeed, skin was less dehydrated for 20 hours after the last application. In addition, Raman experiments showed that AVENE TSW application on SC contributes to high global water content and retention. Besides the water content, the water structure and mobility were of high importance, and the high relative amount of partially bound water compared to tightly bound and unbound water can be related to a better capacity of the water to interact with SC components. In parallel, it maintains high conformational order of the lipid matrix, beneficial for skin barrier function and hydration. Altogether, these data demonstrated how the high moisturizing properties of AVENE TSW topically applied on skin subjects, is associated with SC hydration and barrier function improvement.