Cell Hybrids Research Articles

Director Distribution in a CLC Hybrid Cell with a Small Helicoidal Pitch

Director Distribution in a CLC Hybrid Cell with a Small Helicoidal Pitch

Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion

BackgroundFolates are crucial for the biosynthesis of nucleotides and amino acids, essential for cell proliferation and development. Folate deficiency induces DNA damage, developmental defects, and tumorigenicity. The obligatory enzyme folylpolyglutamate synthetase (FPGS) mediates intracellular folate retention via cytosolic and mitochondrial folate polyglutamylation. Our previous paper demonstrated the association of the cytosolic FPGS (cFPGS) with the cytoskeleton and various cell protrusion proteins. Based on these recent findings, the aim of the current study was to investigate the potential role of cFPGS at cell protrusions.ResultsHere we uncovered a central role for two G-quadruplex (GQ) motifs in the 3′UTR of FPGS mediating the localization of cFPGS mRNA and protein at cell protrusions. Using the MBSV6-loop reporter system and fluorescence microscopy, we demonstrate that following folate deprivation, cFPGS mRNA is retained in the endoplasmic reticulum, whereas upon 15 min of folate repletion, this mRNA is rapidly translocated to cell protrusions in a 3′UTR- and actin-dependent manner. The actin dependency of this folate-induced mRNA translocation is shown by treatment with Latrunculin B and inhibitors of the Ras homolog family member A (RhoA) pathway. Upon folate repletion, the FPGS 3′UTR GQs induce an amoeboid/mesenchymal hybrid cell phenotype during migration and invasion through a collagen gel matrix. Targeted disruption of the 3′UTR GQ motifs by introducing point mutations or masking them by antisense oligonucleotides abrogated cell protrusion targeting of cFPGS mRNA.ConclusionsCollectively, the GQ motifs within the 3′UTR of FPGS regulate its transcript and protein localization at cell protrusions in response to a folate cue, inducing cancer cell invasive phenotype. These novel findings suggest that the 3′UTR GQ motifs of FPGS constitute an attractive druggable target aimed at inhibition of cancer invasion and metastasis.

Enhancement of polyethylene glycol-cell fusion efficiency by novel application of transient pressure using a jet injector.

Cell-cell fusion involves the fusion of somatic cells into a single hybrid cell. It is not only a physiological process but also an important cell engineering technology which can be applied to various fields, such as regenerative medicine, antibody engineering, genetic engineering, and cancer therapy. There are three major methods of cell fusion: electrical cell fusion, polyethylene glycol (PEG) cell fusion, and virus-mediated cell fusion. Although PEG cell fusion is the most economical approach and does not require expensive instrumentation, it has a poor fusion rate and induces a high rate of cell cytotoxicity. To improve the fusion rate of the PEG method, we combined it with the pyro-drive jet injector (PJI). PJI provides instant pressure instead of cell agitation to increase the probability of cell-to-cell contact and shorten the distance between cells in the process of cell fusion. Here, we report that this improved fusion method not only decreased cell cytotoxicity during the fusion process, but also increased fusion rate compared with the conventional PEG method. Furthermore, we tested the functionality of cells fused using the PJI-PEG method and found them to be comparable to those fused using the conventional PEG method in terms of their application for dendritic cell (DC)-tumor cell fusion vaccine production; in addition, the PJI-PEG method demonstrated excellent performance in hybridoma cell preparation. Taken together, our data indicate that this method improves cell fusion efficiency as compared to the PEG method and thus has the potential for use in various applications that require cell fusion technology.

Generation of Stable Epithelial-Mesenchymal Hybrid Cancer Cells with Tumorigenic Potential.

Cancer progression, invasiveness, and metastatic potential have been associated with the activation of the cellular development program known as epithelial-to-mesenchymal transition (EMT). This process is known to yield not only mesenchymal cells, but instead an array of cells with different degrees of epithelial and mesenchymal phenotypes with high plasticity, usually referred to as E/M hybrid cells. The characteristics of E/M hybrid cells, their importance in tumor progression, and the key regulators in the tumor microenvironment that support this phenotype are still poorly understood. In this study, we established an in vitro model of EMT and characterized the different stages of differentiation, allowing us to identify the main genomic signature associated with the E/M hybrid state. We report that once the cells enter the E/M hybrid state, they acquire stable anoikis resistance, invasive capacity, and tumorigenic potential. We identified the hepatocyte growth factor (HGF)/c-MET pathway as a major driver that pushes cells in the E/M hybrid state. Herein, we provide a detailed characterization of the signaling pathway(s) promoting and the genes associated with the E/M hybrid state.

High energy-power characteristics of microstructurally engineered sodium vanadium phosphate in full cell level

The demand for hybrid devices which can deliver high power with large storage capabilities are increasing day-by-day. Sodium based hybrid devices are an appropriate choice to meet the current necessities by considering the abundance, cost and performance. Sodium vanadium phosphate nanocomposite (NC-NVP) has been engineered by making the particles embedded within nitrogen doped mesoporous carbon matrix. Using NC-NVP, a hybrid cell has been devised with activated carbon in an asymmetric cell fashion and compared it with symmetric cell. Asymmetric and symmetric cells have demonstrated cell level energy densities of 77 & 65 and 59 & 46 Wh kg−1 at 0.1 and 1 A g−1 respectively, which are much higher than many reported values. At 2 A g−1, both configurations delivered high power (3722 and 3750 W kg−1) within 1.4 and 1.5 min at retentions of 63 and 51 % after 14,000 cycles respectively. Excellent electrochemical performance suggests that dual mechanism of intercalation in NC-NVP and surface/sub-surface charge storage in nitrogen-doped mesoporous carbon are playing major role. The obtained results are superior to different hybrid devices reported earlier which paves a way for demonstrating sodium-ion device for practical applications.

Computational design of custom therapeutic cells to correct failing human cardiomyocytes.

Myocardial delivery of non-excitable cells-namely human mesenchymal stem cells (hMSCs) and c-kit+ cardiac interstitial cells (hCICs)-remains a promising approach for treating the failing heart. Recent empirical studies attempt to improve such therapies by genetically engineering cells to express specific ion channels, or by creating hybrid cells with combined channel expression. This study uses a computational modeling approach to test the hypothesis that custom hypothetical cells can be rationally designed to restore a healthy phenotype when coupled to human heart failure (HF) cardiomyocytes. Candidate custom cells were simulated with a combination of ion channels from non-excitable cells and healthy human cardiomyocytes (hCMs). Using a genetic algorithm-based optimization approach, candidate cells were accepted if a root mean square error (RMSE) of less than 50% relative to healthy hCM was achieved for both action potential and calcium transient waveforms for the cell-treated HF cardiomyocyte, normalized to the untreated HF cardiomyocyte. Custom cells expressing only non-excitable ion channels were inadequate to restore a healthy cardiac phenotype when coupled to either fibrotic or non-fibrotic HF cardiomyocytes. In contrast, custom cells also expressing cardiac ion channels led to acceptable restoration of a healthy cardiomyocyte phenotype when coupled to fibrotic, but not non-fibrotic, HF cardiomyocytes. Incorporating the cardiomyocyte inward rectifier K+ channel was critical to accomplishing this phenotypic rescue while also improving single-cell action potential metrics associated with arrhythmias, namely resting membrane potential and action potential duration. The computational approach also provided insight into the rescue mechanisms, whereby heterocellular coupling enhanced cardiomyocyte L-type calcium current and promoted calcium-induced calcium release. Finally, as a therapeutically translatable strategy, we simulated delivery of hMSCs and hCICs genetically engineered to express the cardiomyocyte inward rectifier K+ channel, which decreased action potential and calcium transient RMSEs by at least 24% relative to control hMSCs and hCICs, with more favorable single-cell arrhythmia metrics. Computational modeling facilitates exploration of customizable engineered cell therapies. Optimized cells expressing cardiac ion channels restored healthy action potential and calcium handling phenotypes in fibrotic HF cardiomyocytes and improved single-cell arrhythmia metrics, warranting further experimental validation studies of the proposed custom therapeutic cells.

Obtaining and characterization of the spermatogenic cell culture of males from interspecific hybrids of domestic sheep and argal

Relevance. The creation of biological material cryobanks is one of the key methods for the conservation and maintenance of the biodiversity of animal genetic resources. The biomaterial widely used for preservation in cryobank conditions are mature germ cells of males – spermatozoa. As a promising alternative for these purposes is considered the use of testis stem cells – spermatogonia which makes it possible to select biomaterial from immature animals with a valuable genotype. The article presents data on obtaining a culture of spermatogonia of males of interspecific hybrids of domestic sheep with argali. Methods. The object of research was spermatogenic cells of sheep's interspecific hybrids from the Romanov breed with argali. The testes of hybrid males served as a material for obtaining a spermatogenic cells culture. The conditions for isolating and maintaining spermatogonia in culture in vitro were optimized using histological, cytological, immunohistochemical and cultural methods. Results. It has been established that the effectiveness of obtaining a spermatogenic cells culture, maximally enriched with spermatogonia, are affected by the age of the males from which the biomaterial is taken, the preliminary purification of spermatogonia from other types of spermatogenic and somatic testicular cells, the growth medium and the type of feeder layer used for the cultivation of spermatogonia. It is shown that the optimal age of males for the selection of biomaterial is the age period from birth to 4 months. During this period, the cells of the epitheliospermatogenic layer in the seminiferous tubules of the testes from hybrid males are mainly represented by one type of spermatogenic cells – spermatogonia (92–100 %). The maximum purification of spermatogonia from other types of cells is achieved by separating them according to adhesion. High intensity of growth and formation of spermatogonia colonies is observed when they are cultivated on the feeder layer formed by the primary culture of own Sertoli cells, as well as Sertoli cells from another rams. Under these conditions, the attachment of spermatogonia to the cells of the feeder layer is noted on the 1st – 2nd day of cultivation, the formation of colonies – on the 6th day of cultivation.

Endothelial-to-osteoblast transition in normal mouse bone development

Metastatic prostate cancer (PCa) in bone induces bone-forming lesions. We have previously shown that PCa-induced bone originates from endothelial cells (ECs) that have undergone EC-to-osteoblast (OSB) transition. Here, we investigated whether EC-to-OSB transition also occurs during normal bone formation. We developed an EC and OSB dual-color reporter mouse (DRM) model that marks EC-OSB hybrid cells with red and green fluorescent proteins. We observed EC-to-OSB transition (RFP and GFP co-expression) in both endochondral and intramembranous bone formation during embryonic development and in adults. Co-expression was confirmed in cells isolated from DRM. Bone marrow- and lung-derived ECs underwent transition to OSBs and mineralization in osteogenic medium. RNA-sequencing revealed GATA family transcription factors were upregulated in EC-OSB hybrid cells and knockdown of GATA3 inhibited BMP4-induced mineralization. Our findings support that EC-to-OSB transition occurs during normal bone development and suggest a new paradigm regarding the endothelial origin of OSBs.

Comprehensive potentiodynamic analysis of electrodes performance in hybrid capacitors

A methodology which assesses the potentiodynamic characteristics of individual electrodes in hybrid capacitors (HCs) is introduced, using a Li-ion capacitor (LIC) as an example. Each electrode contribution was calculated from cyclic voltammetry (CV) measurements on a cell equipped with a reference electrode by adapting the procedure for electrical double-layer capacitors. It has been confirmed that, during the potentiodynamic analysis of a hybrid capacitor, the scan rate of the electrodes (dE+dt and dE-dt) is not constant in time. Furthermore, dE-dt tends to 0, while dE+dt aims to reach the rate of the voltage sweep (dUdt). By contrast, when the electrodes are analyzed separately at a constant potential scan rate, thevoltammogram of the battery-type negative electrode does not provide the same current response as ahybrid capacitor, where only the voltage ramp is controlled. The proposed methodology is fundamental for revealing any imbalance in the contributions of the electrodes and providing a correct representation of their current profiles during the potentiodynamic analysis of a hybrid cell.

DIRECTOR DISTRIBUTION IN A CLC HYBRID CELL WITH A SMALL HELICOIDAL PITCH

Laser light emission in dye-doped chiral liquid crystals (CLCs) has been experimentally demonstrated for the homeotropic–planar (hybrid) orientation. A numerical simulation of this structure showed that the helicoid (helix) pitch period in CLC in the hybrid cell and the director distribution depend significantly on the anchoring energy at the homeotropic sample boundary. The anchoring energy plays the role of a factor facilitating the CLC helix unwinding. The lower anchoring energy, the smaller the pitch is and the closer to the natural CLC pitch it is. In this case, the length of the “screw"-type structure near the homeotropic cell boundary decreases. Thus, as the anchoring energy at the hybrid-cell homeotropic boundary decreases, the director distribution in the cell approaches the distribution in the planar (Grandjean) cell. This is confirmed by the laser light emission in the same spectral range as in the planar cell.

Obtaining and investigating immunochemical properties of monoclonal antibodies against rCTLA-4 protein

Monoclonal antibodies are used to block control points of the tumor development of many oncological pathologies. One of the critical control points of tumor development of several oncological pathologies is the receptor for cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Monoclonal antibodies against the CTLA-4 receptor are laboratory-derived humanized antibodies. An essential step in the humanization of antibodies is the production of murine hybrid cells producing monoclonal antibodies. This article describes studies of mice monoclonal antibodies against a recombinant human CTLA-4 receptor (rCTLA-4) expressed in Escherichia coli. To obtain strains of hybrid cells producing monoclonal antibodies were used methods of hybridoma technology. As a result, hybrid cells producing monoclonal antibodies to CTLA-4 were obtained. Strains of hybrid cells have high productive activity in vitro and in vivo. Monoclonal antibodies react with rCTLA-4 protein, belong to the class of IgG1, and have a high binding constant. They efficiently bind to the rCTLA-4 receptor and block the interaction of rCTLA-4 with the commercial recombinant human B7-1 Fc and rhesus monkey PD-1 hFc proteins. These monoclonal antibodies to rCTLA-4 can be used to obtain recombinant humanized monoclonal antibodies to the human CTLA-4 receptor.

A cell-surface-anchored DNA probe coupled with hybridization chain reaction enzyme-free dual signal amplification for sensitive electrochemical detection of the cellular microenvironment.

The cellular microenvironment plays key roles in regulating physiological processes. However, it is still a challenge to detect it with quantification. Here, a simple, biocompatible, and universal strategy based on cell surface-anchored specific DNAzymes and hybridization chain reaction enzyme-free signal amplification for cellular microenvironment electrochemical detection is presented. In this strategy, the cell could be captured on the surface of the electrode via aptamer-target recognition. On the other hand, the DNAzyme hybridized with the substrate strand as a metal ion probe was anchored on the surface of the cell. In the presence of metal ions, the substrate strand could be cleaved into two fragments by the DNAzyme and released from the cell surface. Then, the DNA modified gold nanoparticles (AuNPs) could be captured on the electrode. Subsequently, an alternative hybridization reaction of two hairpin probes was triggered by the carried initiators forming nicked double helices. For signal readout, hemin could be inserted into the double-helix DNA long chain via electrostatic interaction, which could electro-reduce hydrogen peroxide to generate an electrochemical signal. Based on the intrinsic advantages of DNAzymes, including rapid kinetics, high sensitivity, and high selectivity, and the signal amplification strategy, this method should be able to monitor and semi-quantify target metal ions in the cellular microenvironment. Furthermore, this method shows potential for various targets by employing different DNA probes in the cellular microenvironment, providing a platform for bioanalysis.

Establishment of the National Centre for E-Vehicle and Sustainable Technology (EVST)

The UK government has set ambitious targets to reduce carbon emissions and combat climate change, with a focus on the widespread adoption of electric vehicles (EVs). Alongside, the government has announced plans to phase out the sale of new petrol and diesel cars by 2030, and to invest in infrastructure such as EV charging stations to support this transition. Additionally, financial incentives, such as grants and tax breaks, are being offered to encourage individuals and businesses to switch to EVs. The adoption of EVs is seen as a key step in achieving the UK's goal of becoming a net-zero carbon economy by 2050. A recent major initiative of the University of Bolton is the establishment of the National Centre for e-Vehicle and Sustainable Technology (EVST). This Centre – established in 2022 – is a collaborative venture between the university group and key partners from industry. Its principal purpose is to provide education and training at a range of levels to support the electric, hybrid and hydrogen cell powered vehicles (both lightweight and heavy) maintenance sector. Whilst all vehicle manufacturers have developed and launched various electric and sustainable models to market, there is a well-recognised skills shortage in the maintenance of these vehicles. A market research exercise was carried out to establish the existing provision of education and training in the UK higher and further education sectors to inform the work in developing the Centre. This exercise provided valuable insight into where the opportunities and gaps existed in this important aspect of enabling widespread adoption of electric and sustainable vehicles to happen in the UK. This paper sets out how EVST will provide the educational base and industry-focussed training for a range of technician and engineer roles to address this skills shortage, initially in the North-West of England and growing to cover the rest of Great Britain.

Multifunctional metal selenide-based materials synthesized via a one-pot solvothermal approach for electrochemical energy storage and conversion applications.

Highly-efficient electroactive materials with distinctive electrochemical features, along with suitable strategies to prepare hetero-nanoarchitectures incorporating two or more transition metal selenides, are currently required to increase charge storage ability. Herein, a one-pot solvothermal approach is used to develop iron-nickel selenide spring-lawn-like architectures (FeNiSe SLAs) on nickel (Ni) foam. The porous Ni foam scaffold not only enables the uniform growth of FeNiSe SLAs but also serves as an Ni source. The effect of reaction time on their morphological and electrochemical properties is investigated. The FeNiSe-15 h electrode shows high areal capacity (493.2 μA h cm-2) and superior cycling constancy. The as-assembled aqueous hybrid cell (AHC) demonstrates high areal capacity and a decent rate capability of 59.4% (50 mA cm-2). The AHC exhibits good energy and power densities, along with excellent cycling stability. Furthermore, to confirm its practicability, the AHC is employed to drive portable electronic appliances by charging it with wind energy. The electrocatalytic activity of FeNiSe-based materials to complete the oxygen evolution reaction (OER) is explored. Among them, the FeNiSe-15 h catalyst shows good OER performance at a current density of 50 mA cm-2. This general synthesis approach may initiate a strategy of advanced metal selenide-based materials for multifunctional applications.

Multicentre experience with Optimus balloon-expandable cobalt–chromium stents in congenital heart disease interventions

ObjectivesTo evaluate bare-metal Optimus and polytetrafluoroethylene (PTFE)-covered Optimus-CVS balloon-expandable, cobalt–chromium, hybrid cell–designed stents in congenital heart disease (CHD) interventions.MethodsRetrospective multicentre review of patients with CHD receiving Optimus stents. Stent mechanical...

Sustainable and effective approach to recover antioxidant compounds from purple tea (Camellia sinensis var. assamica cv. Zijuan) leaves

Camellia sinensis var. assamica cv. Zijuan (purple tea) is known for its content of anthocyanins, flavan-3-ols, and bioactivities. This study aimed to verify the influence of solvent polarity, in a solid–liquid extraction, on the content of phenolic compounds and chlorophylls, instrumental color, and antioxidant activity. Different proportions of water and ethanol (0:100, 25:75, 50:50, 75:25, and 100:0 v/v) were used for extraction. The results showed that the hydroalcoholic extract (75 % ethanol + 25 % water) had the highest contents of total flavonoids, total anthocyanins, chlorophyll A, and total carotenoids, as well as presenting the highest color intensity, proportion of yellow pigments, and antioxidant activity (total reducing capacity and scavenging of the DPPH free radical). Twenty-two compounds were identified, with chlorogenic acid, hesperidin, (-)-epicatechin, (-)-epigallocatechin gallate, and isoquercitrin being the main phenolics. This phenolic-rich extract inhibited lipoperoxidation induced in egg yolk homogenate (IC50 = 455 mg/L), showed no hemolytic behavior when human erythrocytes were subjected to osmotic stress, and exerted in vitro cytotoxic effects against cancer and hybrid cells. The extract obtained with the mixture of non-toxic solvents presented critical bioactivities, as well as a comprehensive identification of phenolic compounds in the cultivar, and has potential to be used in technological applications.

Perspectives of using of somatic cells fusion process in some aspects of car¬cino¬ge-nesis

Normal somatic cells sensitive to carcinogenic effects and capable of proliferation form firstly a binuclear cells (dikaryons) and then hybrid cells (mononucleated synkaryons) by means of fusion with another cells of the same organism, in particular with differentiated and non-differentiated cells of corresponding tissue or with cells capable to migrate. In all probability, during the perforation of the plasma membrane, i.e., after the formation of po­res, induced by different carcinogenic (and noncarcinogenic) agents and factors, the total negative charge of plasma membrane changes (decreases) and the cells develop the ability to come closer to each other, whi­ch will probably be the prerequisite to a fusion process. On the other hand, it is not excluded that perforation can assist the fusion of only neighboring cells. Initially, a set of chromosomes in a precancerous cell retains, even for a short time, the condition of tetraploidy. Because of cell fusion generates tetraploidy, it potentially might cause chromo­somal instability. The article emphasizes a possibility of using the fusion process in different aspects of carcino­genesis (therapy, prevention, etc.).

Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier

Septate junctions (SJs) evolved as cell-cell junctions that regulate the paracellular barrier and integrity of epithelia in invertebrates. Multiple morphological variants of SJs exist specific to different epithelia and/or phyla but the biological significance of varied SJ morphology is unclear because the knowledge of the SJ associated proteins and their functions in non-insect invertebrates remains largely unknown. Here we report cell-specific expression of nine candidate SJ genes in the early life stages of the sea urchin Strongylocentrotus purpuratus. By use of in situ RNA hybridization and single cell RNA-seq we found that the expression of selected genes encoding putatively SJ associated transmembrane and cytoplasmic scaffold molecules was dynamically regulated during epithelial development in the embryos and larvae with different epithelia expressing different cohorts of SJ genes. We focused a functional analysis on SpMesh, a homolog of the Drosophila smooth SJ component Mesh, which was highly enriched in the endodermal epithelium of the mid- and hindgut. Functional perturbation of SpMesh by both CRISPR/Cas9 mutagenesis and vivo morpholino-mediated knockdown shows that loss of SpMesh does not disrupt the formation of the gut epithelium during gastrulation. However, loss of SpMesh resulted in a severely reduced gut-paracellular barrier as quantitated by increased permeability to 3–5 ​kDa FITC-dextran. Together, these studies provide a first look at the molecular SJ physiology during the development of a marine organism and suggest a shared role for Mesh-homologous proteins in forming an intestinal barrier in invertebrates. Results have implications for consideration of the traits underlying species-specific sensitivity of marine larvae to climate driven ocean change.

Trans-differentiation of trophoblast stem cells: implications in placental biology.

Trophoblast invasion is a hallmark of hemochorial placentation. Invasive trophoblast cells replace the endothelial cells of uterine spiral arteries. The mechanism by which the invasive trophoblast cells acquire this phenotype is unknown. Here, we demonstrate that, during differentiation, a small population of trophoblast stem (TS) cells trans-differentiate into a hybrid cell type expressing markers of both trophoblast (TC) and endothelial (EC) cells. In addition, a compendium of EC-specific genes was found to be associated with TS cell differentiation. Using functional annotation, these genes were categorized into angiogenesis, cell adhesion molecules, and apoptosis-related genes. HES1 repressed transcription of EC genes in TS cells. Interestingly, differentiated TCs secrete TRAIL, but its receptor DR4 is expressed only in ECs and not in TCs. TRAIL induced apoptosis in EC but not in TC. Co-culture of ECs with TC induced apoptosis in ECs via extrinsic apoptotic pathway. These results highlight that (a) TS cells possess the potential to trans-differentiate into "trophendothelial" phenotype, regulated by HES1 and (b) trophoblast differentiation-induced TRAIL secretion directs preferential demise of ECs located in their vicinity.

Electroactive CTAB/PVDF composite film based photo-rechargeable hybrid power cell for clean energy generation and storage

Herein, electroactive polymer based photo-induced hybrid power cell has been developed using CTAB/PVDF composite film in a sustainable manner. First high dielectric polymer film has been prepared by doping CTAB in PVDF matrix via solution casting method. In the basic configuration of this hybrid power cell, aqueous electrolyte solution of PVA-MnO2-Eosin Y has been utilized as solar light absorber and photo-electron generator whereas the high dielectric CTAB/PVDF (~ 400) is used as dielectric separator cum storage part in a very transparent way. The cell shows maximum voltage sim 1.1 V with short-circuit current density ~ 7.83 mA/cm2 under ~ 110 mW/cm2 normal light illumination. The device reveal almost same performance for a long time (30 days). The high storage impact of the hybrid cell is investigated by its promising conversion efficiency sim 4.48 % with energy density and power density sim 26.9 mWh/m2 and sim 5.5 W/m2 respectively.