Humans In Europe Research Articles

Animal Welfare in Predator Control: Lessons from Land and Sea. How the Management of Terrestrial and Marine Mammals Impacts Wild Animal Welfare in Human-Wildlife Conflict Scenarios in Europe.

Simple SummaryBoth marine and terrestrial mammal predators come into conflict with humans in Europe and yet their situations are rarely compared. Areas of conflict include the predation of livestock and farmed fish, and also perceived competition for wild prey (for example wolves competing with hunters for deer and seals competing with fishermen for salmon). A lethal method (shooting) and non-lethal methods of conflict reduction used for terrestrial large carnivores (e.g., bear, wolf, wolverine, lynx) and marine mammals (seals) are discussed and their potential impacts on predator welfare are considered. The importance of carrying out an animal welfare assessment when choosing a control method is emphasized along with possible assessment methods. Recommendations for future work are also made.The control of predators, on land and in the sea, is a complex topic. Both marine and terrestrial mammal predators come into conflict with humans in Europe in many ways and yet their situations are rarely compared. Areas of conflict include the predation of livestock and farmed fish, and the perceived competition for wild prey (for example wolves competing with hunters for deer and seals competing with fishermen for salmon). A lethal method (shooting) and non-lethal methods of conflict reduction (including enclosures, guarding, and aversion) used for terrestrial large carnivores (e.g., bear, wolf, wolverine, lynx) and marine mammals (seals) are discussed. Control measures tend to be species- and habitat-specific, although shooting is a widely used method. Potential impacts on predator welfare are described and welfare assessments which have been developed for other wildlife control scenarios, e.g., control of introduced species, are considered for their potential use in assessing predator control. Such assessments should be applied before control methods are chosen so that decisions prioritizing animal welfare can be made. Further work needs to be carried out to achieve appropriate and widely-accepted animal welfare assessment approaches and these should be included in predator management planning. Future research should include further sharing of approaches and information between terrestrial and marine specialists to help ensure that animal welfare is prioritized.

Tick-borne encephalitis in domestic animals.

Tick-borne encephalitis (TBE), a disease caused by tick-borne encephalitis virus (TBEV), represents a serious neural infection of humans in Europe and Asia. The main reservoir hosts of TBEV are ticks, rodents and insectivores, but domestic animals may also be infected with the virus. This review summarizes what is known about TBE in domestic species (e.g., dogs, horses), in which infection may manifest with clinical signs similar to those seen in severe human cases. We also focus on TBE in ruminants where TBE infections are typically asymptomatic and do not cause health problems in the infected hosts. However, the risk to human health is the main problem of asymptomatic infection, because the presence of TBEV in the milk of infected ruminants can serve as a source of TBE infection via the alimentary route. An experimental veterinary vaccine was developed recently, and future vaccination of selected domestic animals is proposed to avoid the development of severe TBE symptoms in sensitive animals (e.g., dogs, horses) or to decrease the risk of alimentary infection in humans (e.g., goats and sheep). Keywords: tick-borne encephalitis; dog; goat; sheep; cow; horse.

Monitoring of vector-borne diseases in the west part of Ukraine

During recent years, infectious and invasive animal diseases caused by viruses, bacteria, and protozoa and transmitted by ticks have been a new problem in medical and veterinary practice. Many of these diseases are zoonoses and lead to the disability and mortality of humans and animals. Ixodes ricinus and Dermacentor reticulatus are species of ticks that are commonly attack animals and humans in Europe. These ticks are spread throughout the EU and are involved in the transmission of a large number of vector-borne diseases. To date, relatively limited data on the circulation of tick-borne diseases and their transmission in western Ukraine are presented. The purpose of this study was to monitor the types of ixodid ticks, as well as to identify pathogens in ticks collected from the environment in urban parks and from dogs and cats. A total of 215 ticks (22 Ixodes ricinus and 193 Dermacentor reticulatus) collected in two regions of Lviv and Ivano-Frankivsk were investigated. Pathogens, namely, Rikettsia spp., Candidatus Neoehrlichia mikurensis, Anaplasma phagocytophilum and Bartonella spp., were detected in 64.0% of the I. ricinus ticks. Overall, 18.2% of I. ricinus ticks were positive for Rikettsia spp., 54.5% for Candidatus Neoehrlichia mikurensis, 9.1% for A. phagocytophilum and Bartonella spp. confirmed in 4.5% of the studied ticks. Mixed infestations were found in 5 samples of I. ricinus DNA, representing 22.7%. In addition, 74.6% of D. reticulatus ticks were infested with these pathogens. Overall, 30.0% of D. reticulatus ticks were positive for Rikettsia spp., Candidatus Neoehrlichia mikurensis was confirmed in 54.9%, A. phagocytophilum in 1.6%, and Bartonella spp. in 6.2% of the studied ticks. Mixed infestations were found in 35 D. reticulatus DNA samples, representing 18.1%. The prevalence of Rikettsia spp. in two regions of Lviv and Ivano-Frankivsk, among the studied ticks was 28.8%, Bartonella spp. – 6.0%, A. phagocytophilum – 2.3%, Candidatus Neoehrlichia mikurensis – 54.9%. Our data indicate the presence of pathogens in urban populations of ticks of I. ricinus and D. reticulatus in west part of Ukraine. Monitoring of tick-borne diseases is an important tool in the prevention and control of infections transmitted to humans and animals.

Lithic techno-complexes in Italy from 50 to 39 thousand years BP: An overview of lithic technological changes across the Middle-Upper Palaeolithic boundary

Defining the processes involved in the technical/cultural shifts from the Late Middle to the Early Upper Palaeolithic in Europe (~50-39 thousand years BP) is one of the most important tasks facing prehistoric studies. Apart from the technological diversity generally recognised as belonging to the latter part of the Middle Palaeolithic, some assemblages showing original technological traditions (i.e. Initial Upper Palaeolithic: Bohunician, Bachokirian; so called transitional industries: Châtelperronian, Szeletian, Lincombian-Ranisian-Jerzmanowician, Uluzzian; Early Upper Palaeolithic: Protoaurignacian, Early Aurignacian) first appear during this interval.Explaining such technological changes is a crucial step in order to understand if they were the result of the arrival of new populations, the result of parallel evolution, or of long-term processes of cultural and biological exchanges.In this debate Italy plays a pivotal role, due to its geographical position between eastern and western Mediterranean Europe as well as to it being the location of several sites showing Late Mousterian, Uluzzian and Protoaurignacian evidence distributed across the Peninsula.Our study aims to provide a synthesis of the available lithic evidence from this key area through a review of the evidence collected from a number of reference sites. The main technical features of the Late Mousterian, the Uluzzian and the Protoaurignacian traditions are examined from a diachronic and spatial perspective.Our overview allows the identification of major differences in the technological behaviour of these populations, making it possible to propose a number of specific working hypotheses on the basis of which further studies can be carried out.This study presents a detailed comparative study of the whole corpus of the lithic production strategies documented during this interval, and crucial element thus emerge: 1. In the Late Mousterian tools were manufactured with great attention being paid to the production phases and with great investment in inizializing and managing core convexities; 2. In contrast, Uluzzian lithic production proceeded with less careful management of the first phases of debitage, mainly obtaining tool morphologies by retouching. 3. In the Protoaurignacian the production is carefully organized and aimed at obtaining laminar blanks (mainly bladelets) usually marginally retouched.These data are of primary importance in order to assess the nature of the "transition" phenomenon in Italy, thus contributing to the larger debate about the disappearance of Neandertals and the arrival of early Modern Humans in Europe.

Prehistoric babies drank animal milk

Around 9,000 years ago, humans in Europe started to settle down, plant crops, and raise animals. They also had a baby boom. Chemists led by Julie Dunne of the University of Bristol have now found evidence that by the first century BCE parents were weaning their children with animal milk (Nature 2019, DOI: 10.1038/s41586-019-1572-x). “This is the first direct evidence that babies were bottle-fed ruminant milk in prehistoric Europe,” says Siân Halcrow, an expert in childhood archaeology at the University of Otago who wasn’t involved in the study In 2016, Dunne was scrolling through Facebook when a post caught her eye. The post was from the “Motherhood in Prehistory” page, run by Katharina Rebay-Salisbury at the Institute for Oriental and European Archaeology, and it described prehistoric feeding vessels. These vessels are small and have a spout that someone could use to pour liquid into a mouth or that a child

Direct radiocarbon dates of mid Upper Palaeolithic human remains from Dolní Věstonice II and Pavlov I, Czech Republic

The ritual human burials and scattered fragments of human bones excavated from Dolní Věstonice II and Pavlov I (Czech Republic) in the 20th century provide a large body of evidence on morphology and funerary practices in the Gravettian as well as the population history of European Homo sapiens during the Upper Palaeolithic. A series of radiocarbon dates on charcoal and animal bone places the occupation of the sites predominantly between 31,000–29,000 cal BP (Early-Evolved Pavlovian) but direct radiocarbon dating of the human remains has not been previously undertaken. In 2013, human bones from Dolní Věstonice II and Pavlov I were sampled for aDNA analysis, including three skeletons from a triple burial (DV13, DV14, DV15), two skeletons from single burials (Pav1, DV16) and two unarticulated human bones (DV42, DV43). Small amounts of bone material were left over from the aDNA sampling, providing the first opportunity to directly date seven of the human individuals. Non-destructive pre-screening with near-infrared (NIR) spectroscopy indicated that sufficient collagen was preserved in the bone material for radiocarbon dating. We sampled very small amounts (32–202 mg) of bone material for collagen extraction, ultrafiltration and accelerator mass spectrometer (AMS) dating. Each collagen extract was dated multiple times using both graphite targets (ca. 800 μg C) and the gas ion source (<100 μg C) of the AixMICADAS to obtain accurate and precise radiocarbon ages. The direct dates confirm the Pavlovian origin of the human remains and indicate that several of the radiocarbon dates carried out in the 1980s on associated charcoals were likely affected by low-level contamination of modern carbon. The results add seven individuals to the small collection of reliably dated Upper Palaeolithic humans in Europe.

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Notes on Contributors

Large-scale mitogenomic analysis of the phylogeography of the Late Pleistocene cave bear

The cave bear (Ursus spelaeus) is one of the Late Pleistocene megafauna species that faced extinction at the end of the last ice age. Although it is represented by one of the largest fossil records in Europe and has been subject to several interdisciplinary studies including palaeogenetic research, its fate remains highly controversial. Here, we used a combination of hybridisation capture and next generation sequencing to reconstruct 59 new complete cave bear mitochondrial genomes (mtDNA) from 14 sites in Western, Central and Eastern Europe. In a Bayesian phylogenetic analysis, we compared them to 64 published cave bear mtDNA sequences to reconstruct the population dynamics and phylogeography during the Late Pleistocene. We found five major mitochondrial DNA lineages resulting in a noticeably more complex biogeography of the European lineages during the last 50,000 years than previously assumed. Furthermore, our calculated effective female population sizes suggest a drastic cave bear population decline starting around 40,000 years ago at the onset of the Aurignacian, coinciding with the spread of anatomically modern humans in Europe. Thus, our study supports a potential significant human role in the general extinction and local extirpation of the European cave bear and illuminates the fate of this megafauna species.

Detection of CTX-M-1 extended-spectrum beta-lactamase among ceftiofur-resistant Salmonella enterica clinical isolates of poultry.

Salmonella enterica resistance to extended-spectrum cephalosporins (ESC) conferred by cefotaximases (blaCTX-M) is a growing concern in the United States. Among food-producing animals, poultry are a major reservoir of ESC-resistant Salmonella. A retrospective study was carried out to further characterize 38 ceftiofur-resistant clinical Salmonella enterica isolates obtained from poultry during 2007-2018. Of the isolates tested, 31 displayed resistance to ceftriaxone and harbored blaCMY-2, whereas 7 isolates demonstrated resistance or reduced susceptibility to cefepime in addition to ceftriaxone resistance. These 7 isolates displayed extended-spectrum β-lactamase activity, harbored blaCTX-M-1, and were recovered only from recent poultry diagnostic submissions made in 2011-2018 as opposed to the 31 isolates that were recovered in 2007-2018. Further characterization of the blaCTX-M-1 gene determined that it was located on conjugative IncN/ST1 and IncI1/ST87 plasmids in the isolates from commercial turkeys and broilers, respectively. These plasmids have been responsible for extensive spread of blaCTX-M-1 in livestock, poultry, and humans in Europe. Potential transfer of IncN and IncI1 plasmids and/or nontyphoidal Salmonella carrying these plasmids through the food chain, or by other means to humans, may result in treatment failures. Our study demonstrates the importance of further characterization of ceftiofur-resistant S. enterica isolates detected by veterinary diagnostic laboratories to identify the sources of blaCTX-M-1 and to mitigate the spread of ESC-resistant Salmonella in the poultry production pyramid.

Comparing fitness and drift explanations of Neanderthal replacement.

There is a general consensus among archaeologists that replacement of Neanderthals by anatomically modern humans in Europe occurred around 40-35 ka. However, the causal mechanism for this replacement continues to be debated. Proposed models have featured either fitness advantages in favour of anatomically modern humans or invoked neutral drift under various preconditions. Searching for specific fitness advantages in the archaeological record has proven difficult, as these may be obscured, absent or subject to interpretation. To bridge this gap, we rigorously compare the system-level properties of fitness- and drift-based explanations of Neanderthal replacement. Our stochastic simulations and analytical predictions show that, although both fitness and drift can produce replacement, they present important differences in (i) required initial conditions, (ii) reliability, (iii) time to replacement, and (iv) path to replacement (population histories). These results present useful opportunities for comparison with archaeological and genetic data. We find greater agreement between the available empirical evidence and the system-level properties of replacement by differential fitness, rather than by neutral drift.

Reconciling the Greenland ice-core and radiocarbon timescales through the Laschamp geomagnetic excursion

Cosmogenic radionuclides, such as 10Be and 14C, share a common production signal, with their formation in the Earth's upper atmosphere modulated by changes to the geomagnetic field, as well as variations in the intensity of the solar wind. Here, we use this common production signal to compare between the radiocarbon (IntCal) and Greenland ice-core (GICC05) timescales, utilising the most pronounced cosmogenic production peak of the last 100,000 years – that associated with the Laschamp geomagnetic excursion circa 41,000 years ago. We present 54 new 14C measurements from a peat core (‘TP-2005’) from Tenaghi Philippon, NE Greece, contiguously spanning between circa 47,300 and 39,600 cal. BP, demonstrating a distinctive tripartite structure in the build up to the principal Laschamp production maximum that is not present in the consensus IntCal13 calibration curve. This is the first time that a continuous, non-reservoir corrected 14C dataset has been generated over such a long time span for this, the oldest portion of the radiocarbon timescale. This period is critical for both palaeoenvironmental and archaeological applications, with the replacement of Neanderthals by anatomically modern humans in Europe around this time. By placing our Tenaghi Philippon 14C dataset on to the Hulu Cave U-series timescale of Cheng et al. (2018) via Bayesian statistical modelling, the comparison of TP-2005 14C with Greenland 10Be fluxes also implicitly relates the underlying U-series and GICC05 timescales themselves. This comparison suggests that whilst these two timescales are broadly coherent, the IntCal13 timescale contains erroneous structure circa 40,000 cal. BP.

The effect of sublethal concentrations of deltamethrin and alphacypermethrin on the fecundity and development of Ixodes ricinus (Acari: Ixodidae) eggs and larvae

Two pyrethroids, deltamethrin (D) and alphacypermethrin (AC), have been used as commercial products for tick control worldwide. However, the effects of sublethal doses of these compounds on various tick species and their developmental stages have not been fully explored yet, although such knowledge could contribute to a more effective application of both pyrethroids and simultaneous reduction of their costs and undesirable side effects, including the harmful impact on other organisms and environmental contamination. This study investigates the effect of sublethal concentrations of D and AC applied to engorged females on the fecundity, development of eggs and larvae, and the number of offspring in the Ixodes ricinus (L.) tick, which is the most important vector of tick-borne diseases of humans in Europe. After detachment from rabbit’s skin, fully engorged I. ricinus females were treated with 20 μl of pyrethroid solutions at five concentrations from 0.01562 to 0.25% and kept at 28 °C and 75% relative humidity. The impact of the pyrethroids on maturation and development of eggs as well as development of larvae was assessed based on parameters of the preoviposition and oviposition periods and the course of embryogenesis and egg hatch. The investigations have shown that both tested acaricides reduce the reproductive rate in I. ricinus females by inhibition of egg development and disturb embryonic development and larval hatch. Females did not lay eggs at concentrations higher than 0.0625% AC and 0.125% D. The lower concentrations of both pyrethroids disturbed or inhibited the embryogenesis and egg hatch in I. ricinus.

Seropositivity to Midichloria mitochondrii (order Rickettsiales) as a marker to determine the exposure of humans to tick bite

ABSTRACTIxodes ricinus is the most common tick species parasitizing humans in Europe, and the main vector of Borrelia burgdorferi sensu lato, the causative agent of Lyme disease in the continent. This tick species also harbors the endosymbiont Midichloria mitochondrii, and there is strong evidence that this bacterium is inoculated into the vertebrate host during the blood meal. A high proportion of tick bites remains unnoticed due to rarity of immediate symptoms, implying the risk of occult tick-borne infections in turn a potential risk factor for the onset of chronic-degenerative diseases. Since suitable tools to determine the previous exposure to I. ricinus bites are needed, this work investigated whether seropositivity toward a protein of M. mitochondrii (rFliD) could represent a marker for diagnosis of I. ricinus bite. We screened 274 sera collected from patients from several European countries, at different risk of tick bite, using an ELISA protocol. Our results show a clear trend indicating that positivity to rFliD is higher where the tick bite can be regarded as certain/almost certain, and lower where there is an uncertainty on the bite, with the highest positivity in Lyme patients (47.30%) and the lowest (2.00%) in negative controls. According to the obtained results, M. mitochondrii can be regarded as a useful source of antigens, with the potential to be used to assess the exposure to ticks harboring this bacterium. In prospect, additional antigens from M. mitochondrii and tick salivary glands should be investigated and incorporated in a multi-antigen test for tick bite diagnosis.

Stable isotopes reveal patterns of diet and mobility in the last Neandertals and first modern humans in Europe

Correlating cultural, technological and ecological aspects of both Upper Pleistocene modern humans (UPMHs) and Neandertals provides a useful approach for achieving robust predictions about what makes us human. Here we present ecological information for a period of special relevance in human evolution, the time of replacement of Neandertals by modern humans during the Late Pleistocene in Europe. Using the stable isotopic approach, we shed light on aspects of diet and mobility of the late Neandertals and UPMHs from the cave sites of the Troisième caverne of Goyet and Spy in Belgium. We demonstrate that their diet was essentially similar, relying on the same terrestrial herbivores, whereas mobility strategies indicate considerable differences between Neandertal groups, as well as in comparison to UPMHs. Our results indicate that UPMHs exploited their environment to a greater extent than Neandertals and support the hypothesis that UPMHs had a substantial impact not only on the population dynamics of large mammals but also on the whole structure of the ecosystem since their initial arrival in Europe.

New evidence of broader diets for archaic Homo populations in the northwestern Mediterranean.

Investigating diet breadth is critical for understanding how archaic Homo populations, including Neanderthals, competed for seasonally scarce resources. The current consensus in Western Europe is that ungulates formed the bulk of the human diet during the Lower and Middle Paleolithic, while small fast prey taxa were virtually ignored. Here, we present a multisite taphonomic study of leporid assemblages from Southern France that supports frequent exploitation of small fast game during marine isotope stages 11 to 3. Along with recent evidence from Iberia, our results indicate that the consumption of small fast game was more common prior to the Upper Paleolithic than previously thought and that archaic hominins from the northwestern Mediterranean had broader diets than those from adjacent regions. Although likely of secondary importance relative to ungulates, the frequent exploitation of leporids documented here implies that human diet breadths were substantially more variable within Europe than assumed by current evolutionary models.

Usutu Virus Isolated from Rodents in Senegal

Usutu virus (USUV) is a Culex-associated mosquito-borne flavivirus of the Flaviviridae family. Since its discovery in 1959, the virus has been isolated from birds, arthropods and humans in Europe and Africa. An increasing number of Usutu virus infections in humans with neurological presentations have been reported. Recently, the virus has been detected in bats and horses, which deviates from the currently proposed enzootic cycle of USUV involving several different avian and mosquito species. Despite this increasing number of viral detections in different mammalian hosts, the existence of a non-avian reservoir remains unresolved. In Kedougou, a tropical region in the southeast corner of Senegal, Usutu virus was detected, isolated and sequenced from five asymptomatic small mammals: Two different rodent species and a single species of shrew. Additional molecular characterization and in vivo growth dynamics showed that these rodents/shrew-derived viruses are closely related to the reference strain (accession number: AF013412) and are as pathogenic as other characterized strains associated with neurological invasions in human. This is the first evidence of Usutu virus isolation from rodents or shrews. Our findings emphasize the need to consider a closer monitoring of terrestrial small mammals in future active surveillance, public health, and epidemiological efforts in response to USUV in both Africa and Europe.

Exceptionally high δ15N values in collagen single amino acids confirm Neandertals as high-trophic level carnivores

Isotope and archeological analyses of Paleolithic food webs have suggested that Neandertal subsistence relied mainly on the consumption of large herbivores. This conclusion was primarily based on elevated nitrogen isotope ratios in Neandertal bone collagen and has been significantly debated. This discussion relies on the observation that similar high nitrogen isotopes values could also be the result of the consumption of mammoths, young animals, putrid meat, cooked food, freshwater fish, carnivores, or mushrooms. Recently, compound-specific C and N isotope analyses of bone collagen amino acids have been demonstrated to add significantly more information about trophic levels and aquatic food consumption. We undertook single amino acid C and N isotope analysis on two Neandertals, which were characterized by exceptionally high N isotope ratios in their bulk bone or tooth collagen. We report here both C and N isotope ratios on single amino acids of collagen samples for these two Neandertals and associated fauna. The samples come from two sites dating to the Middle to Upper Paleolithic transition period (Les Cottés and Grotte du Renne, France). Our results reinforce the interpretation of Neandertal dietary adaptations as successful top-level carnivores, even after the arrival of modern humans in Europe. They also demonstrate that high δ15N values of bone collagen can solely be explained by mammal meat consumption, as supported by archeological and zooarcheological evidence, without necessarily invoking explanations including the processing of food (cooking, fermenting), the consumption of mammoths or young mammals, or additional (freshwater fish, mushrooms) dietary protein sources.

A quantitative risk assessment for human Taenia solium exposure from home slaughtered pigs in European countries

BackgroundTaenia solium, a zoonotic tapeworm, is responsible for about a third of all preventable epilepsy human cases in endemic regions. In Europe, adequate biosecurity of pig housing and meat inspection practices have decreased the incidence of T. solium taeniosis and cysticercosis. Pigs slaughtered at home may have been raised in suboptimal biosecurity conditions and slaughtered without meat inspection. As a result, consumption of undercooked pork from home slaughtered pigs could pose a risk for exposure to T. solium. The aim of this study was to quantify the risk of human T. solium exposure from meat of home slaughtered pigs, in comparison to controlled slaughtered pigs, in European countries. A quantitative microbial risk assessment model (QMRA) was developed and porcine cysticercosis prevalence data, the percentage of home slaughtered pigs, meat inspection sensitivity, the cyst distribution in pork and pork consumption in five European countries, Bulgaria, Germany, Poland, Romania and Spain, were included as variables in the model. This was combined with literature about cooking habits to estimate the number of infected pork portions eaten per year in a country.ResultsThe results of the model showed a 13.83 times higher prevalence of contaminated pork portions from home slaughtered pigs than controlled slaughtered pigs. This difference is brought about by the higher prevalence of cysticercosis in pigs that are home raised and slaughtered. Meat inspection did not affect the higher exposure from pork that is home slaughtered. Cooking meat effectively lowered the risk of exposure to T. solium-infected pork.ConclusionsThis QMRA showed that there is still a risk of obtaining an infection with T. solium due to consumption of pork, especially when pigs are reared and slaughtered at home, using data of five European countries that reported porcine cysticercosis cases. We propose systematic reporting of cysticercosis cases in slaughterhouses, and in addition molecularly confirming suspected cases to gain more insight into the presence of T. solium in pigs and the risk for humans in Europe. When more data become available, this QMRA model could be used to evaluate human exposure to T. solium in Europe and beyond.

Limits of long-term selection against Neandertal introgression

Several studies have suggested that introgressed Neandertal DNA was subjected to negative selection in modern humans. A striking observation in support of this is an apparent monotonic decline in Neandertal ancestry observed in modern humans in Europe over the past 45,000 years. Here, we show that this decline is an artifact likely caused by gene flow between modern human populations, which is not taken into account by statistics previously used to estimate Neandertal ancestry. When we apply a statistic that avoids assumptions about modern human demography by taking advantage of two high-coverage Neandertal genomes, we find no evidence for a change in Neandertal ancestry in Europe over the past 45,000 years. We use whole-genome simulations of selection and introgression to investigate a wide range of model parameters and find that negative selection is not expected to cause a significant long-term decline in genome-wide Neandertal ancestry. Nevertheless, these models recapitulate previously observed signals of selection against Neandertal alleles, in particular the depletion of Neandertal ancestry in conserved genomic regions. Surprisingly, we find that this depletion is strongest in regulatory and conserved noncoding regions and in the most conserved portion of protein-coding sequences.

Current threat of rabies in Europe and in the world

Rabies still remains one of the most serious diseases of mankind and poses a serious worldwide public health problem. The introduction of dog vaccination as well as oral vaccination of wildlife in many countries has proved to be an effective method of eradication of rabies in non-flying animals. As a result of these actions many countries have been freed from rabies. However, the illegal movement of pet animals poses a serious risk of reintroducing the virus into the rabies-free area. Although, the prevalence of rabies in humans in Europe is relatively low, half of the world's population lives in endemic areas. A number of international organisations are working together on a global framework for eliminating dog-mediated human rabies.