Purpose: Manual procurement processes are expensive in terms of human resource cost, purchasing cost, wastage due to errors of commission or omissions, cause of delays in delivery of goods, services, and works which accounts for 40% of losses due to inefficiencies, and attracts corruption in the system amongst others. The study aimed to assess the effect of e-tendering on performance of humanitarian United Nations agencies and non-governmental organizations in South Sudan. Methodology: The study methodology adopted an explanatory research design of cross-sectional type for the population under study. There were over 235 National Non-governmental organizations, 150 International organizations in addition to over 15 United Nations agencies, bringing the total target population to 400 organizations. This study targeted 400 humanitarian organizations operating in South Sudan. Therefore, the sample size of 200 humanitarian organizations operating in South Sudan was selected using a stratified random sampling technique. Data was collected using structured questionnaires and analyzed using descriptive and inferential statistics. Results: Reliability findings indicated that e-tendering had a Cronbach Alpha Coefficient of 0.873, and performance had 0.859. The study established that e-tendering had a positive and significant relationship with performance significant (β=0.282, P=0.000). Conclusion: The study concluded that e-tendering has a positive and significant effect on performance of humanitarian United Nations agencies and non-governmental organizations. This study hence suggested that all NGOs and humanitarian agencies should ensure they adopt the use of e-tendering in their solicitation and sourcing processes in conducting procurement activities. The study cited advantages such saving time, wide coverage of markets and reaching more potential suppliers and finally save environment from printing more paper works attaining sustainable procurement practice.
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