Response Of Human Organism Research Articles

Solubility and ADMET profiles of short oligomers of lactic acid.

Polylactic acid (PLA) is a polymer with an increased potential to be used in different medical applications, including tissue engineering and drug-carries. The use of PLA in medical applications implies the evaluation of the human organism's response to the polymer inserting and to its degradation products. Consequently, within this study, we have investigated the solubility and ADMET profiles of the short oligomers (having the molecular weight lower than 3000 Da) resulting in degradation products of PLA. There is a linear decrease of the molar solubility of investigated oligomers with molecular weight. The results that are obtained also reveal that short oligomers of PLA have promising pharmacological profiles and limited toxicological effects on humans. These oligomers are predicted as potential inhibitors of the organic anion transporting peptides OATP1B1 and OATP1B3, they present minor probability to affect the androgen and glucocorticoid receptors, have a weak potential of hepatotoxicity, and may produce eye injuries. These outcomes may be used to guide or to supplement in vitro and/or in vivo toxicity tests such as to enhance the biodegradation properties of the biopolymer.

Influence of honeybee sting on peptidome profile in human serum.

The aim of this study was to explore the serum peptide profiles from honeybee stung and non-stung individuals. Two groups of serum samples obtained from 27 beekeepers were included in our study. The first group of samples was collected within 3 h after a bee sting (stung beekeepers), and the samples were collected from the same person a second time after at least six weeks after the last bee sting (non-stung beekeepers). Peptide profile spectra were determined using MALDI-TOF mass spectrometry combined with Omix, ZipTips and magnetic beads based on weak-cation exchange (MB-WCX) enrichment strategies in the mass range of 1–10 kDa. The samples were classified, and discriminative models were established by using the quick classifier, genetic algorithm and supervised neural network algorithms. All of the statistical algorithms used in this study allow distinguishing analyzed groups with high statistical significance, which confirms the influence of honeybee sting on the serum peptidome profile. The results of this study may broaden the understanding of the human organism’s response to honeybee venom. Due to the fact that our pilot study was carried out on relatively small datasets, it is necessary to conduct further proteomic research of the response to honeybee sting on a larger group of samples.

Evidence for proangiogenic cellular and humoral systemic response in patients with acute onset of spinal cord injury

Context/objectiveTraumatic spinal cord injury (SCI) leads to disruption of local vasculature inducing secondary damage of neural tissue. Circulating endothelial progenitor cells (EPCs) play an important role in post-injury regeneration of vasculature, whereas endothelial cells (ECs) reflect endothelial damage.MethodsTwenty patients with SCI were assessed during the first 24 hours, at day 3, and day 7 post-injury and compared to 25 healthy subjects. We herein investigated EPC and EC counts by flow cytometry as well as the levels of soluble factors (SDF-1, HGF, VEGF, Ang2, EGF, endoglin, PLGF, FGF-2, ET-1, BDNF, IGF-1) regulating their migration and proangiogenic function. To better characterize peripheral blood (PB) cells, global gene expression profiles of PB-derived cells were determined using genome-wide RNA microarray technology.ResultsWe found significantly higher EPC (CD34+/CD133+/VEGFR2+) as well as EC (VEGFR2+) count in PB of patients with SCI within 7 days post-injury and the increased HGF, ET-1, Ang2, EGF, and PLGF plasma levels. Global gene expression analysis revealed considerably lower expression of genes associated with both innate and adaptive immune response in PB cells in patients.ConclusionCollectively, our findings demonstrate that SCI triggers bone marrow-derived EPC mobilization accompanied by increased circulating EC numbers. Significant changes in both chemoattractive and proangiogenic cytokines plasma levels occurring rapidly after SCI suggest their role in SCI-related regenerative responses to injury. Broadened knowledge concerning the mechanisms governing of human organism response to the SCI might be helpful in developing effective therapeutic strategies.

Effects of high altitude exposure on plasma and urinary digoxin-like immunoreactive substance.

Six young healthy subjects underwent a 20 day exposure to altitude, at 4930 m (16,174 ft), to evaluate possible plasma and urine digoxin-like immunoreactive substance (DLIS) changes accompanying the altered water and electrolyte balance induced by hypoxia. We studied DLIS, plasma renin activity (PRA), aldosterone, atrial natriuretic peptide (ANP), and arginine vasopressin (ADH) in serial blood and urine samples. An increase in DLIS in plasma (P less than .005) and urine (P less than .01) was found, while aldosterone was decreased (P less than .02). PRA, ADH, and ANP did not change significantly. A trend to a greater loss of sodium through urinary excretion, correlated with urinary DLIS values (r = 0.47, P less than .01), was observed. Data suggest a possible important role of DLIS in adaptive response of human organism to high altitude.

Low levels effect of nitrogen oxides emitted by power station

Abstract In some people exposed to the long‐lasting activity of compounds contained in the “ chimney trail “ of power station the increasel level of MetHb was found but no increased values of SHb were observed Human organism responds to low concentrations of nitrogen oxides contained in the atmosphere by reaction to increased level of MetHb as well as the increase of GSH concentration in erythro‐cytes found near the upper limits of norm, and the increase of activity of G‐6‐PD and first of all the activity of MetHb‐Red. In the examined cases the Hb moleculary structure was of A type≪ The mechanism of human organism's response to the low concentrations of nitrogen oxides in inhaled air may be examined in two aspects, namely as the subtoxic effect with the increase of MetHb and as hormetic activity, stimulating the increase of reduction system activity.

The current status of hypothermia.

THERE are rumbles in the background suggesting that a storm is brewing—that a new concept of the significance of the human organism's response to trauma is evolving which might lead to new techniques for solving many major problems in the management of the sick patient. From France one hears of artificial hibernation, of slow-motion life, of sympathetic neuroplegia; in Britain hypotensive anesthesia has become common practice; in the United States general hypothermia is creeping into the armamentarium of the cardiac surgeon. What is the conceptual relationship among these techniques, if any, and what is the evidence to support them? What biological kinship exists between the pharmacologic lytic cocktail and the physical agent ice-water? Herein, at the present time, lies the confusion of a welter of undocumented theories. Yet an underlying hypothesis of great attractiveness appears dimly, seeking to be established or refuted, not by claims and rash clinical trial,