Abstract
The aim of this study was to explore the serum peptide profiles from honeybee stung and non-stung individuals. Two groups of serum samples obtained from 27 beekeepers were included in our study. The first group of samples was collected within 3 h after a bee sting (stung beekeepers), and the samples were collected from the same person a second time after at least six weeks after the last bee sting (non-stung beekeepers). Peptide profile spectra were determined using MALDI-TOF mass spectrometry combined with Omix, ZipTips and magnetic beads based on weak-cation exchange (MB-WCX) enrichment strategies in the mass range of 1–10 kDa. The samples were classified, and discriminative models were established by using the quick classifier, genetic algorithm and supervised neural network algorithms. All of the statistical algorithms used in this study allow distinguishing analyzed groups with high statistical significance, which confirms the influence of honeybee sting on the serum peptidome profile. The results of this study may broaden the understanding of the human organism’s response to honeybee venom. Due to the fact that our pilot study was carried out on relatively small datasets, it is necessary to conduct further proteomic research of the response to honeybee sting on a larger group of samples.
Highlights
The diagnostic algorithm for Hymenoptera venom hypersensitivity is a serious issue in allergological practice
The first group of samples was collected within 3 h after a bee sting
MALDI-TOF MS combined with Omix, ZipTips and magnetic beads based on weak-cation exchange (MB-WCX) enrichment strategies were used in the study to detect LMW protein/peptide profile spectra in the mass range of 1–10 kDa
Summary
The diagnostic algorithm for Hymenoptera venom hypersensitivity (insect sting allergy) is a serious issue in allergological practice. Measurement of specific IgE-antibodies’ (sIgE) concentration and skin tests represent the routinely used methods to demonstrate the response of the organism to the honeybee sting [1,2]. These tests are not sufficient for a proper diagnosis, because of their non-specificity. A number of prospective studies analyzing the biochemistry response of the organism to the venom have been published in the last decade [1,2,3,4,5,6]. There is still a lack of work analyzing the human organism’s response to Hymenoptera sting at the proteomic or peptidomic level
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