Objective: Most studies have conducted human gait analysis using expensive and invasive photogrammetric systems. The objective of this study was to demonstrate that non-invasive and cost-effective systems based on depth cameras may be able to retrieve relevant features of human gait patterns. We aimed to prove this by solving the problem of gait classification by gender. Methods: 81 participants (40 female and 41 male) walked at a self-selected speed across a 4.8-meter walkway. Gait data was recorded using multiple depth sensors. Analysis in time domain included joint excursions by gait phases, range of movement (ROM), central tendency and dispersion measures, spatial variables, and center of mass (COM) position. The spectral analysis included principal frequency, magnitude, and phase shift during walking. Only features with significant differences by gender were used to train a support vector machine (SVM) classifier. Results: A total of 108 features presented significant differences by gender (p<; 0.05). On this basis, the accuracy of the chosen model was 96.7%. Trunk rotation, trunk sway, knee abduction/adduction, and pelvic obliquity were the most differentiated between the groups. The COM position shown a significant difference by gender (p=0.0065) with 51.7% and 51.0% for men and women respectively. Women proved to have significantly shorter normalized step width than men (p=0.0472). Conclusion: The proposed method was able to retrieve most of human gait features correctly, including differences in gait pattern by gender. Significance: Depth cameras represent a cost-effective system that could be used for a deeper biomechanical human gait analysis.