Various factors including vibration levels influenced ride comfort recognized by passengers during transits by high-speed trains. The vibration level was measured on the floor of the train. The measurement on the surface on the seat cushion and the seatback is also being considered for evaluations. The frequency weightings were applied to evaluate the ride comfort. In this study, three axis vibration on the passenger body was measured to analyze the human body vibration and its effect on the ride comfort. The vibration levels were measured for passengers in the seated and the standing positions. The vibrations of the seat and the human body were measured at constant speed of 300 ㎞/h. The frequency weightings defined in ISO 2631-1 were applied to the body vibration levels for finding the ride comfort. The ride comfort in the seated position was compared to that in the standing position using the proposed level. The comparison shows information about the importance of the vibration measurement locations for ride comfort evaluation.