Responding to thermal comfort based on physiological indicators is a broad and reliable research method. How to use skin temperature to predict thermal comfort in a non-uniform environment is of great significance. 24 volunteers were selected for testing with local radiant heating in Kitakyushu, Japan. It was found that the most obvious skin temperature difference value occurred between left and right thighs while heating after 30 min, which would exceed 5 °C. The asymmetric thermal sensation votings (ATSV) and the skin temperature difference were significantly influenced while changing radiation intensity and radiation distance, the ATSV of the subjects in the small space was about 0.3 lower than that of the subjects in the large space. Increasing the radiation intensity, the TSV of the small space decreased by about −0.5, which can be seen that higher radiation intensity may bring lower value of the TSV. A prediction model of the ATSV and skin temperature difference was established and the sensitivity analysis was performed on the different body parts that significantly affected the ATSV, and the dominant factors affecting the ATSV were obtained. This paper complements the non-uniform thermal environment prediction method.